LOCUS       NM_019715               3251 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus potassium channel modulatory factor 1 (Kcmf1),
            transcript variant 1, mRNA.
ACCESSION   NM_019715 XM_001477544
VERSION     NM_019715.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Beilke,S., Oswald,F., Genze,F., Wirth,T., Adler,G. and Wagner,M.
  TITLE     The zinc-finger protein KCMF1 is overexpressed during pancreatic
            cancer development and downregulation of KCMF1 inhibits pancreatic
            cancer development in mice
  JOURNAL   Oncogene 29 (28), 4058-4067 (2010)
   PUBMED   20473331
  REMARK    GeneRIF: KCMF1 mediates pro-oncogenic functions in vitro and in
            vivo and downregulation of KCMF1 results in the inhibition of
            pancreatic cancer formation in mice
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Guo,G., Huss,M., Tong,G.Q., Wang,C., Li Sun,L., Clarke,N.D. and
            Robson,P.
  TITLE     Resolution of cell fate decisions revealed by single-cell gene
            expression analysis from zygote to blastocyst
  JOURNAL   Dev Cell 18 (4), 675-685 (2010)
   PUBMED   20412781
REFERENCE   3  (bases 1 to 3251)
  AUTHORS   Sato,A., Sekine,Y., Saruta,C., Nishibe,H., Morita,N., Sato,Y.,
            Sadakata,T., Shinoda,Y., Kojima,T. and Furuichi,T.
  TITLE     Cerebellar development transcriptome database (CDT-DB): profiling
            of spatio-temporal gene expression during the postnatal development
            of mouse cerebellum
  JOURNAL   Neural Netw 21 (8), 1056-1069 (2008)
   PUBMED   18603407
REFERENCE   4  (bases 1 to 3251)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   5  (bases 1 to 3251)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3251)
  AUTHORS   Li,Z., Stuart,R.O., Eraly,S.A., Gittes,G., Beier,D.R. and
            Nigam,S.K.
  TITLE     Debt91, a putative zinc finger protein differentially expressed
            during epithelial morphogenesis
  JOURNAL   Biochem Biophys Res Commun 306 (3), 623-628 (2003)
   PUBMED   12810064
  REMARK    GeneRIF: Data suggest that Debt91 is an immediate early gene
            up-regulated during growth factor-induced branching tubulogenesis.
REFERENCE   7  (bases 1 to 3251)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 3251)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153613.9, AK142436.1 and CX734813.1.
            
            On or before Oct 23, 2008 this sequence version replaced
            XM_001477544.1, NM_019715.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.67876.1,
                                           SRR1660811.148328.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-244               AC153613.9         45704-45947
            245-2963            AK142436.1         2-2720
            2964-3251           CX734813.1         143-430
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 32.3 cM"
     gene            1..3251
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /note="potassium channel modulatory factor 1"
                     /db_xref="GeneID:74287"
                     /db_xref="MGI:MGI:1921537"
     exon            1..366
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /inference="alignment:Splign:2.1.0"
     CDS             351..1496
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     potassium channel modulatory factor DEBT-91; E3
                     ubiquitin-protein ligase KCMF1; debt-91; differentially
                     expressed in branching tubulogenesis 91; clone DEBT-91;
                     RING-type E3 ubiquitin transferase KCMF1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase KCMF1 isoform 1"
                     /protein_id="NP_062689.2"
                     /db_xref="CCDS:CCDS39518.1"
                     /db_xref="GeneID:74287"
                     /db_xref="MGI:MGI:1921537"
                     /translation="MSRHEGVSCDACLKGNFRGRRYKCLICYDYDLCASCYESGATTT
                     RHTTDHPMQCILTRVDFDLYYGGEAFSVEQPQSFTCPYCGKMGYTETSLQEHVTSEHA
                     ETSTEVICPICAALPGGDPNHVTDDFAAHLTLEHRAPRDLDESSGVRHVRRMFHPGRG
                     LGGPRARRSNMHFTSSSTGGLSSSQSSYSPSSREAMDPIAELLSQLSGVRRSAGGQLN
                     SSGPSASQLQQLQMQLQLERQHAQAARQQLETARNASRRTNTSSVTTTITQATATANT
                     ANTENSPQALHNSQFLLTRLNDPKMSEAERQSMESERADRSLFVQELLLSTLVREESS
                     SSDEDDRGEMADFGAMGCVDIMPLDVALENLNLKESNKGNEPPPPPL"
     exon            367..534
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /inference="alignment:Splign:2.1.0"
     exon            535..674
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /inference="alignment:Splign:2.1.0"
     exon            675..776
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /inference="alignment:Splign:2.1.0"
     exon            777..951
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1234
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..3251
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1828..1833
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /note="hexamer: AATAAA"
     polyA_site      1856
                     /gene="Kcmf1"
                     /gene_synonym="1700094M07Rik; Debt91; Pmcf"
                     /note="major polyA site"
ORIGIN      
        1 gaggggacga gcgggatttg cggacgccgc gccgctgccg ggagccggcg ggggcccgag
       61 agtgaccgag tcacggcggg cgccggcgga gccgcggccg cgtcggaccc gcctcctgga
      121 ggagctcagc cccggccagg cccggcccca ttcccgcccc gcgccgcctc cccgccgccg
      181 ccgccgccgc cgccgcggga gcgctcccct gcccaccccg cccccgcggc cgagcccggg
      241 agtcgagtgg gagtcggccg gccgggcgcg ggcagcgccg ggagcccgcg gtgggggcac
      301 actgcagccg ggagcccggg aggggccgcg ccgccagcgt ctgaactagg atgtcccgac
      361 atgaaggtgt cagctgtgat gcatgtttaa aaggaaattt tcgaggtcgc agatataagt
      421 gtttaatttg ctacgattac gatctttgtg catcttgtta tgaaagcggg gcaacgacaa
      481 caaggcatac aactgaccac ccgatgcagt gcatattaac aagggtagac tttgatttgt
      541 actatggtgg ggaagctttc tctgtagagc agccacagtc ttttacttgt ccctattgtg
      601 gaaaaatggg ctacacggaa acatctcttc aagaacatgt tacttctgaa cacgcagaaa
      661 catcaacaga agtgatttgt cccatatgtg cagcgttacc tggaggagat cctaatcatg
      721 tcacagatga ctttgcagct catctgacac ttgaacacag agcccctaga gatttagatg
      781 aatcgagtgg tgttcgacat gtacgtagaa tgtttcaccc tgggcggggg ctaggaggcc
      841 ctcgtgctcg gagatcaaac atgcacttta ctagcagttc tactggtgga ctctcttctt
      901 ctcagagttc atactctcca agcagtaggg aagccatgga tcctatagct gagctgttat
      961 ctcagttatc aggagtgaga cgttctgcag ggggacagct taactcttca ggcccttccg
     1021 cttctcagtt gcaacaactg cagatgcagc tgcagcttga gcgacagcat gcacaggcag
     1081 cccggcaaca actggagact gctcgcaacg caagtcggcg cactaacaca agcagcgtca
     1141 ccactacgat cacacaggcc acggccacag ccaacacagc caacacagag aacagcccgc
     1201 aggccctcca caactcccag ttcctgttaa caaggttgaa tgatcccaaa atgtctgaag
     1261 cagagcgcca gtccatggag agcgagcgtg cggaccgcag cctgtttgtc caggagctcc
     1321 ttctttccac gttagtgcgc gaagagagct cgtcctcaga tgaggatgat cggggggaga
     1381 tggcagattt tggtgctatg ggctgtgtag atattatgcc tttagatgtt gctttagaaa
     1441 acctaaattt aaaagagagt aataaaggaa atgagcctcc accacctcct ctttgatgac
     1501 ctccccactc gaagacaatg tcctctgtgc tgtatttgcc aatgaaagtg gacaacagct
     1561 atcctgggtt tgtgtggtga ttgtaatttc aggtctgtca ctcttgttac attgtgtaca
     1621 ttcaaaagga agagagaaat atatatgata atcatttcca cttaactaat ttttacttct
     1681 agcaggtaaa tgtaggtagc agtgcagggt gatctctgct tcctgtacct tgacatgcaa
     1741 aaggctctcc taatactcca cattcaaact gaagaggaaa attgaaatct ctaatgaagc
     1801 tgctgtgtgt atttatgaat attaatgaat aaaaactgct tggatggttt acctaactac
     1861 tgcatgaggt tttttgcagc gtgcatgagt ttcagtggcc ttgttattta aaaattaaat
     1921 acaaggagta aaacttaaaa aaataaaatt taaaaataaa aataaaatac aaagcccaaa
     1981 gctttcccag cgttatccaa tggttactgg cagagtagct tttgaataat gtctgcctga
     2041 ctcaccctta tccatgtgcc tcctacgcac aaagccagct ctgcagtgga acccaggggg
     2101 cctagccagc tacagcgctc cactgtatga ctgtcctgtc gccctgttaa ccagtccagc
     2161 ctgtctctgt ctcttgtctc accagaagac acaccagtaa aggcggggtc ctctgcaggg
     2221 cgactgtgtg ccctttagac aaatcctgtt tgtgtttgtt ggaagttttt actttttgtg
     2281 gttgtttaaa attttttgat tgttaaacat gttcattcag gtgtagatgg atttcattta
     2341 ttcactgttc agtgcagtta acctgaatta tgttgtcttg ttttttaaaa tctcaccacc
     2401 tcaatcatac tttgtgccgt ttatgtgtgt gtgtggtttg ctgagacaga tcagtatcag
     2461 gggagccttc ccacacctta ccaaccaaat tcttaatgct tattttagca ccttgtattt
     2521 attgtttttc ccctcctggc ataaaaagta aagtttttta attgattcat gccacccata
     2581 tgcctatgtt atcaatcctg tgtgttgaaa aaagaaaaaa gtagagctgc cttggtagtt
     2641 agggtgtgga ggatgggtgc tttctgtttg tctccaggtt tcacactggc tccagggagg
     2701 ggcagaggct ctgcgggggt ggggcagttt ttgaaatgta aatttagaac ttttaaaaaa
     2761 gatgcgcagc ttctgatcaa tttctatgag acttaatcta atcatgtctg gctccggccc
     2821 tcttccctgt gagccgtccg tcctctgttg gttctctctc ccgccatctt gtcctcccct
     2881 ctgtttttca tgacaagcat acagacttga acacccctgg gtgctgtcaa gaactgtgaa
     2941 gtccatggtc atccaaatct accaaaaaaa gaagtcaccc tccatgtcta aaaaaactgt
     3001 cgcttctcct cggaaacttg agactccacc agcttccaga tacagtagct gttttcttta
     3061 gtctttgatg gaaatcttct gaaggaaccg ccctcgctgg gctcctggta caccagaggt
     3121 caaggccggt ggggttttct ttttttaatt ggttgttgag aagtttcaga agttcgtttt
     3181 ccagctgtgg tcatttcaga cacatctgca ccataactca cacatttcaa taaagcattt
     3241 tcaagactgt t
//
