LOCUS       NM_053092               2045 bp    mRNA    linear   ROD 19-AUG-2024
DEFINITION  Mus musculus lysyl-tRNA synthetase 1 (Kars1), transcript variant 2,
            mRNA.
ACCESSION   NM_053092
VERSION     NM_053092.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Yu,L., Chen,Z., Zhou,X., Teng,F., Bai,Q.R., Li,L., Li,Y., Liu,Y.,
            Zeng,Q., Wang,Y., Wang,M., Xu,Y., Tang,X. and Wang,X.
  TITLE     KARS Mutations Impair Brain Myelination by Inducing Oligodendrocyte
            Deficiency: One Potential Mechanism and Improvement by Melatonin
  JOURNAL   J Pineal Res 76 (5), e12998 (2024)
   PUBMED   39087379
  REMARK    GeneRIF: KARS Mutations Impair Brain Myelination by Inducing
            Oligodendrocyte Deficiency: One Potential Mechanism and Improvement
            by Melatonin.
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2045)
  AUTHORS   Sleigh,J.N., Mech,A.M., Aktar,T., Zhang,Y. and Schiavo,G.
  TITLE     Altered Sensory Neuron Development in CMT2D Mice Is Site-Specific
            and Linked to Increased GlyRS Levels
  JOURNAL   Front Cell Neurosci 14, 232 (2020)
   PUBMED   32848623
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2045)
  AUTHORS   Jiang,D., Burger,C.A., Casasent,A.K., Albrecht,N.E., Li,F. and
            Samuel,M.A.
  TITLE     Spatiotemporal gene expression patterns reveal molecular
            relatedness between retinal laminae
  JOURNAL   J Comp Neurol 528 (5), 729-755 (2020)
   PUBMED   31609468
REFERENCE   5  (bases 1 to 2045)
  AUTHORS   Lim,H.X., Jung,H.J., Lee,A., Park,S.H., Han,B.W., Cho,D. and
            Kim,T.S.
  TITLE     Lysyl-Transfer RNA Synthetase Induces the Maturation of Dendritic
            Cells through MAPK and NF-kappaB Pathways, Strongly Contributing to
            Enhanced Th1 Cell Responses
  JOURNAL   J Immunol 201 (9), 2832-2841 (2018)
   PUBMED   30275047
  REMARK    GeneRIF: these results indicated that KRS effectively induced the
            maturation and activation of dendritic cells
REFERENCE   6  (bases 1 to 2045)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   7  (bases 1 to 2045)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   8  (bases 1 to 2045)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   9  (bases 1 to 2045)
  AUTHORS   Maas,S., Kim,Y.G. and Rich,A.
  TITLE     Genomic clustering of tRNA-specific adenosine deaminase ADAT1 and
            two tRNA synthetases
  JOURNAL   Mamm Genome 12 (5), 387-393 (2001)
   PUBMED   11331948
REFERENCE   10 (bases 1 to 2045)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114648.25, AK167693.1 and W77297.1.
            
            On Nov 3, 2013 this sequence version replaced NM_053092.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon compared to variant 4. The resulting isoform
            (2) is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036289.1, AK167693.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                AC114648.25        65381-65456         c
            77-1993             AK167693.1         2-1918
            1994-2045           W77297.1           388-439
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 58.27 cM"
     gene            1..2045
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /note="lysyl-tRNA synthetase 1"
                     /db_xref="GeneID:85305"
                     /db_xref="MGI:MGI:1934754"
     exon            1..145
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1874
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /EC_number="6.1.1.6"
                     /note="isoform 2 is encoded by transcript variant 2;
                     lysine--tRNA ligase"
                     /codon_start=1
                     /product="lysine--tRNA ligase isoform 2"
                     /protein_id="NP_444322.1"
                     /db_xref="CCDS:CCDS40481.1"
                     /db_xref="GeneID:85305"
                     /db_xref="MGI:MGI:1934754"
                     /translation="MATLQESEVKVDGEQKLSKNELKRRLKAEKKLAEKEAKQKELSE
                     KQLNQTASAPNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVTGEDPYPHKFHVDIS
                     LTQFIQEYSHLQPGDHLTDVTLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRN
                     YKSEEEFVHINNKLRRGDIIGVEGNPGKTKKGELSIIPQEITLLSPCLHMLPHLHFGL
                     KDKETRYRQRYLDLILNDFVRQKFIVRSKIITYIRSFLDELGFLEIETPMMNIIPGGA
                     VAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFT
                     TCEFYMAYADYHDLMEITEKMLSGMVKSITGSYKITYHPDGPEGQAYEVDFTPPFRRI
                     SMVEELEKALGVKLPETSLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFL
                     EVTCISPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPVRQRQ
                     LFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVL
                     LFPAMKPEDKKETAATTETPESTEASPSV"
     exon            146..302
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            303..468
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            469..562
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            563..749
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            750..875
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            876..995
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1158
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1332
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1418
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1504
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1631
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1775
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..2043
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2019..2024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /note="hexamer: AATAAA"
     polyA_site      2039
                     /gene="Kars1"
                     /gene_synonym="D8Ertd698e; D8Wsu108e; Kars; LysRS;
                     mKIAA0070"
                     /note="major polyA site"
ORIGIN      
        1 gaggcaggac ttagagccac gacctggaag ttctagttct acgctagtca taattttttt
       61 ttttctgggt cgggccagtg gggaagatgg ccacgctgca ggagagtgaa gtcaaagtgg
      121 atggcgagca gaaactaagc aagaatgagc tgaagaggcg tctgaaagct gagaagaaac
      181 tggcagagaa ggaggccaag cagaaagagc tgagtgagaa acagctaaac cagactgctt
      241 ccgctcccaa ccacacggct gacaatggcg tgggtgctga ggaggagact ctggacccaa
      301 atcaatacta caagatccga agtcaagccg tccagcagct gaaggtcact ggggaggatc
      361 cgtacccaca caagttccac gtggacatct cactcactca gttcatccaa gaatatagtc
      421 acctgcagcc tggggaccac ctgactgatg tcaccctcaa agtggcaggc cgcatccacg
      481 ccaagagggc ctctggaggg aagctcatct tctatgacct gcgaggagag ggggtcaagt
      541 tacaagtcat ggccaactcc aggaattaca aatcagagga ggaatttgtt catatcaata
      601 acaaactgcg ccggggagac ataattggag ttgagggcaa tcccgggaaa accaagaagg
      661 gcgagctgag catcatcccc caggagatca cgctgctgtc cccctgcttg cacatgctgc
      721 ctcatcttca ctttggcctc aaagacaagg aaacacggta tcgtcaaaga tacttggact
      781 tgatcctgaa cgactttgtg aggcagaaat ttatcgtccg ctctaagatc atcacataca
      841 taagaagttt cttggatgag ctgggcttcc tagagatcga aactcccatg atgaacatca
      901 ttcccggggg agctgtggcc aagcctttca tcacctatca caatgagctg gacatgaact
      961 tgtatatgag aattgctcca gaactctacc acaagatgct ggtggttggt ggcattgacc
     1021 gggtttatga aattgggcgc cagttccgga acgaagggat tgatttgact cacaatcctg
     1081 agttcaccac ctgtgagttc tacatggcct atgcagacta tcatgacctc atggagatca
     1141 cagagaagat gctgtcaggg atggtgaaga gcattacagg cagttacaag atcacctacc
     1201 acccagatgg gccggaaggc caagcctacg aggtcgactt caccccaccc ttccgaagaa
     1261 tcagcatggt agaagagctt gagaaagccc taggtgtgaa gctgccagaa accagtctct
     1321 ttgaaactga agaaactcgg aaaattcttg atgatatttg tgttgcaaaa gctgttgaat
     1381 gccccccacc tcggaccaca gccaggctcc ttgataagct cgttggcgag ttcctcgaag
     1441 tcacatgcat cagccctacc ttcatctgtg atcacccaca gatcatgagt cctttggcca
     1501 aatggcaccg ctccaaagag ggtctcacgg agcgctttga gctgtttgtc atgaagaagg
     1561 agatatgcaa tgcctatact gagctgaatg accccgtgcg gcagaggcag ctgtttgagg
     1621 agcaggccaa ggccaaggct gccggtgatg acgaggccat gttcatagac gagaacttct
     1681 gtactgccct ggaatacggg ctgcctccca cagcgggctg gggcatgggc attgatcggc
     1741 tcaccatgtt tctcaccgat tccaacaata tcaaggaagt acttctgttt cctgccatga
     1801 agccagagga caagaaggaa actgcagcga ccactgaaac cccagagagc acagaggcca
     1861 gcccctctgt ctagaaggca catgcaacta tggtgtctgc atctcagcag atctggtctg
     1921 agggagcgat tcctgtgtgg cgccgtccat ctggccactg cagtccaacc tccacaagag
     1981 agaagaacta aagaagttct ttttattcct cgttaccaaa taaatcattt ctctctttac
     2041 ccaaa
//
