LOCUS       NM_001418984            1355 bp    mRNA    linear   ROD 14-OCT-2024
DEFINITION  Mus musculus izumo sperm-egg fusion 1 (Izumo1), transcript variant
            2, mRNA.
ACCESSION   NM_001418984 XM_036153469
VERSION     NM_001418984.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Brukman,N.G., Nakajima,K.P., Valansi,C., Flyak,K., Li,X.,
            Higashiyama,T. and Podbilewicz,B.
  TITLE     A novel function for the sperm adhesion protein IZUMO1 in cell-cell
            fusion
  JOURNAL   J Cell Biol 222 (2) (2023)
   PUBMED   36394541
  REMARK    GeneRIF: A novel function for the sperm adhesion protein IZUMO1 in
            cell-cell fusion.
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Contreras,W., Wiesehofer,C., Schreier,D., Leinung,N., Peche,P.,
            Wennemuth,G., Gentzel,M., Schroder,B. and Mentrup,T.
  TITLE     C11orf94/Frey is a key regulator for male fertility by controlling
            Izumo1 complex assembly
  JOURNAL   Sci Adv 8 (32), eabo6049 (2022)
   PUBMED   35960805
REFERENCE   3  (bases 1 to 1355)
  AUTHORS   Matsumura,T., Noda,T., Satouh,Y., Morohoshi,A., Yuri,S., Ogawa,M.,
            Lu,Y., Isotani,A. and Ikawa,M.
  TITLE     Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma
            in Mice and Rats
  JOURNAL   Front Cell Dev Biol 9, 810118 (2022)
   PUBMED   35096839
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1355)
  AUTHORS   Inoue,N., Hagihara,Y. and Wada,I.
  TITLE     Evolutionarily conserved sperm factors, DCST1 and DCST2, are
            required for gamete fusion
  JOURNAL   Elife 10, e66313 (2021)
   PUBMED   33871360
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1355)
  AUTHORS   Inoue,N., Saito,T. and Wada,I.
  TITLE     Unveiling a novel function of CD9 in surface compartmentalization
            of oocytes
  JOURNAL   Development 147 (15) (2020)
   PUBMED   32665248
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1355)
  AUTHORS   Wang,M., Lv,Z., Shi,J., Hu,Y. and Xu,C.
  TITLE     Immunocontraceptive potential of the Ig-like domain of Izumo
  JOURNAL   Mol Reprod Dev 76 (8), 794-801 (2009)
   PUBMED   19288544
  REMARK    GeneRIF: Data indicate that the Ig-like domain of Izumo plays an
            important role in the fertilization process, as verified by the
            dose-dependent reduction in fertilization rates in mouse IVF trials
            and mouse mating assay.
            Erratum:[Mol Reprod Dev. 2009 Aug;76(8):802]
REFERENCE   7  (bases 1 to 1355)
  AUTHORS   Miranda,P.V., Allaire,A., Sosnik,J. and Visconti,P.E.
  TITLE     Localization of low-density detergent-resistant membrane proteins
            in intact and acrosome-reacted mouse sperm
  JOURNAL   Biol Reprod 80 (5), 897-904 (2009)
   PUBMED   19144954
REFERENCE   8  (bases 1 to 1355)
  AUTHORS   An,G., Huang,T.H., Wang,D.G., Xie,Q.D., Ma,L. and Chen,D.Y.
  TITLE     In vitro and in vivo studies evaluating recombinant plasmid
            pCXN2-mIzumo as a potential immunocontraceptive antigen
  JOURNAL   Am J Reprod Immunol 61 (3), 227-235 (2009)
   PUBMED   19239425
  REMARK    GeneRIF: pCXN2-mIzumo plasmid possesses appreciable anti-fertility
            potential.
REFERENCE   9  (bases 1 to 1355)
  AUTHORS   Inoue,N., Ikawa,M. and Okabe,M.
  TITLE     Putative sperm fusion protein IZUMO and the role of N-glycosylation
  JOURNAL   Biochem Biophys Res Commun 377 (3), 910-914 (2008)
   PUBMED   18952059
  REMARK    GeneRIF: These data suggest that glycosylation is not essential for
            the function of IZUMO, but has a role in protecting it from
            fragmentation in cauda epididymis.
REFERENCE   10 (bases 1 to 1355)
  AUTHORS   Inoue,N., Ikawa,M., Isotani,A. and Okabe,M.
  TITLE     The immunoglobulin superfamily protein Izumo is required for sperm
            to fuse with eggs
  JOURNAL   Nature 434 (7030), 234-238 (2005)
   PUBMED   15759005
  REMARK    GeneRIF: identification of a mouse sperm fusion-related antigen and
            show that the antigen is a novel immunoglobulin superfamily protein
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149057.7.
            
            On Mar 27, 2023 this sequence version replaced XM_036153469.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: LC426749.1, SRR5189685.4213.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AC149057.7         46667-46731         c
            66-375              AC149057.7         45751-46060         c
            376-450             AC149057.7         45414-45488         c
            451-537             AC149057.7         44561-44647         c
            538-558             AC149057.7         44100-44120         c
            559-639             AC149057.7         43773-43853         c
            640-740             AC149057.7         43560-43660         c
            741-894             AC149057.7         43087-43240         c
            895-1153            AC149057.7         42501-42759         c
            1154-1355           AC149057.7         41553-41754         c
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.39 cM"
     gene            1..1355
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /note="izumo sperm-egg fusion 1"
                     /db_xref="GeneID:73456"
                     /db_xref="MGI:MGI:1920706"
     exon            1..65
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..1334
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /note="isoform 2 is encoded by transcript variant 2; izumo
                     sperm-egg fusion protein 1; OBF; oocyte binding/fusion
                     factor; sperm-specific protein Izumo"
                     /codon_start=1
                     /product="izumo sperm-egg fusion protein 1 isoform 2"
                     /protein_id="NP_001405913.1"
                     /db_xref="GeneID:73456"
                     /db_xref="MGI:MGI:1920706"
                     /translation="MLKFFQRPPPCPLFGVRGSSGGAGKLCVLAFMGPHFTLLLAALA
                     NCLCPGRPCIKCDQFVTDALKTFENTYLNDHLPHDIHKNVMRMVNHEVSSFGVVTSAE
                     DSYLGAVDENTLEQATWSFLKDLKRITDSDLKGELFIKELLWMLRHQKDIFNNLARQF
                     QKEVLCPNKCGVMSQTLIWCLKCEKQLHICRKSLDCGERHIEVHRSEDLVLDCLLSWH
                     RASKGLTDYSFYRVWENSSETLIAKGKEPYLTKSMVGPEDAGNYRCVLDTINQGHATV
                     IRYDVTVLPPKHSEENQPPNIITQEEHETPVHVTPQTPPGQEPESELYPELHPELYPE
                     LIPTVAQNPEKKMKTRLLILLTLGFVVLVASIIISVLHFRKVSAKLKNASDEVKPTAS
                     GSKSDQSLSQQMGLKKASQADFNSDYSGDKSEATEN"
     exon            66..375
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            376..450
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            451..537
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            538..558
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            559..639
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            640..740
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            741..894
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1153
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1355
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1333..1338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /note="hexamer: AATAAA"
     polyA_site      1355
                     /gene="Izumo1"
                     /gene_synonym="1700058F15Rik; Izumo"
                     /note="major polyA site"
ORIGIN      
        1 ggaagagtag acaggtaccc cagagactgg aggatggctt tggaggcatg ctgaagtttt
       61 tccagcgccc tcccccctgc cctctgtttg gggtgagagg atcctctggc ggtgctggca
      121 agctgtgcgt tctggccttc atggggccgc attttacact cttgctggca gctcttgcca
      181 actgcctgtg tccagggagg ccctgcatca aatgtgacca gtttgtgaca gatgcgctaa
      241 agactttcga aaacacttac ctgaatgacc acctgccaca cgacattcac aaaaatgtaa
      301 tgaggatggt gaaccatgaa gtatcgagct tcggcgtagt cacttcggct gaggattcct
      361 atttgggggc cgtggacgag aacacactgg aacaagcaac ctggagtttt ctgaaggatc
      421 tgaagcgtat tacagacagt gacttaaaag gagagctctt tataaaggaa ctattgtgga
      481 tgcttcgtca tcaaaaggac atctttaaca atcttgctag acagttccaa aaggaagttc
      541 tttgtcccaa caaatgcgga gtgatgtcgc agactttgat ctggtgtctt aagtgcgaaa
      601 agcagttgca catttgtcgg aaatccctag attgtggaga gcgccacata gaggtacatc
      661 gctcggaaga cctggtcctg gactgtctgc tcagttggca tcgtgcttct aagggactta
      721 cagattacag tttttacagg gtttgggaga acagttctga gaccttgatt gccaagggga
      781 aagaaccata tctgaccaag tcgatggtgg gtccagagga tgctggcaac taccgctgtg
      841 tgctagatac catcaaccaa ggtcatgcca ccgtcatccg ctacgatgtc acagtattgc
      901 ccccaaagca ttcagaggaa aaccaaccac cgaacatcat aacccaagag gagcacgaga
      961 ctcctgtcca cgtgactcca cagacaccac cggggcagga gccagagtcg gagctgtacc
     1021 cggagctgca cccagagttg tacccggagc tcatacctac agtcgctcaa aatccagaga
     1081 aaaaaatgaa aactcgcctc ttaatactgt tgacccttgg ctttgtggtt cttgtggcca
     1141 gcatcattat ctcggtactt cactttagga aagtcagcgc taaattgaag aatgcaagtg
     1201 acgaagtcaa acccaccgcg tcaggatcca agtcagatca gagtttgtcc caacagatgg
     1261 gattgaagaa ggcctctcag gcagatttta actctgacta ctctggagat aagagcgagg
     1321 caacagaaaa ctaataaaga tttgtatttg actta
//
