LOCUS       NM_011365               6001 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus intersectin 2 (Itsn2), transcript variant 2, mRNA.
ACCESSION   NM_011365
VERSION     NM_011365.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Vollweiter,D., Shergill,J.K., Hilse,A., Kochlamazashvili,G.,
            Koch,S.P., Mueller,S., Boehm-Sturm,P., Haucke,V. and Maritzen,T.
  TITLE     Intersectin deficiency impairs cortico-striatal neurotransmission
            and causes obsessive-compulsive behaviors in mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (35), e2304323120 (2023)
   PUBMED   37603735
REFERENCE   3  (bases 1 to 6001)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6001)
  AUTHORS   Locard-Paulet,M., Voisinne,G., Froment,C., Goncalves Menoita,M.,
            Ounoughene,Y., Girard,L., Gregoire,C., Mori,D., Martinez,M.,
            Luche,H., Garin,J., Malissen,M., Burlet-Schiltz,O., Malissen,B.,
            Gonzalez de Peredo,A. and Roncagalli,R.
  TITLE     LymphoAtlas: a dynamic and integrated phosphoproteomic resource of
            TCR signaling in primary T cells reveals ITSN2 as a regulator of
            effector functions
  JOURNAL   Mol Syst Biol 16 (7), e9524 (2020)
   PUBMED   32618424
  REMARK    GeneRIF: LymphoAtlas: a dynamic and integrated phosphoproteomic
            resource of TCR signaling in primary T cells reveals ITSN2 as a
            regulator of effector functions.
REFERENCE   5  (bases 1 to 6001)
  AUTHORS   Nishino,T., Tamada,K., Maeda,A., Abe,T., Kiyonari,H., Funahashi,Y.,
            Kaibuchi,K., Takumi,T. and Konishi,H.
  TITLE     Behavioral analysis in mice deficient for GAREM2 (Grb2-associated
            regulator of Erk/MAPK subtype2) that is a subtype of highly
            expressing in the brain
  JOURNAL   Mol Brain 12 (1), 94 (2019)
   PUBMED   31718706
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6001)
  AUTHORS   Choi,Y.S. and Sinha,S.
  TITLE     Determination of the consensus DNA-binding sequence and a
            transcriptional activation domain for ESE-2
  JOURNAL   Biochem J 398 (3), 497-507 (2006)
   PUBMED   16704374
  REMARK    GeneRIF: The isolation and characterization of ESE2 is reported.
REFERENCE   7  (bases 1 to 6001)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   8  (bases 1 to 6001)
  AUTHORS   Yoshizawa,M., Sone,M., Matsuo,N., Nagase,T., Ohara,O., Nabeshima,Y.
            and Hoshino,M.
  TITLE     Dynamic and coordinated expression profile of dbl-family guanine
            nucleotide exchange factors in the developing mouse brain
  JOURNAL   Gene Expr Patterns 3 (3), 375-381 (2003)
   PUBMED   12799088
REFERENCE   9  (bases 1 to 6001)
  AUTHORS   Okamoto,M., Schoch,S. and Sudhof,T.C.
  TITLE     EHSH1/intersectin, a protein that contains EH and SH3 domains and
            binds to dynamin and SNAP-25. A protein connection between
            exocytosis and endocytosis?
  JOURNAL   J Biol Chem 274 (26), 18446-18454 (1999)
   PUBMED   10373452
REFERENCE   10 (bases 1 to 6001)
  AUTHORS   Sengar,A.S., Wang,W., Bishay,J., Cohen,S. and Egan,S.E.
  TITLE     The EH and SH3 domain Ese proteins regulate endocytosis by linking
            to dynamin and Eps15
  JOURNAL   EMBO J 18 (5), 1159-1171 (1999)
   PUBMED   10064583
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC241534.2, BC138262.1 and BC037119.1.
            
            On Mar 23, 2012 this sequence version replaced NM_011365.3.
            
            Transcript Variant: This variant (2) lacks an in-frame alternate
            exon in the 5' coding region, compared to variant 1, which results
            in a shorter isoform (2), compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.95301.1,
                                           SRR10223971.12485.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-195               AC241534.2         204244-204438
            196-2123            BC138262.1         173-2100
            2124-5549           BC138262.1         2182-5607
            5550-6001           BC037119.1         1176-1627
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 2.09 cM"
     gene            1..6001
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="intersectin 2"
                     /db_xref="GeneID:20403"
                     /db_xref="MGI:MGI:1338049"
     exon            1..225
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            226..288
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     CDS             258..5234
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="isoform 2 is encoded by transcript variant 2; EH
                     and SH3 domains protein 2; SH3 domain-containing protein
                     1B; EH domain and SH3 domain regulator of endocytosis 2;
                     SH3 domain protein 1B; Eh domain, SH3 domain regulator of
                     endocytosis 2"
                     /codon_start=1
                     /product="intersectin-2 isoform 2"
                     /protein_id="NP_035495.2"
                     /db_xref="CCDS:CCDS36398.1"
                     /db_xref="GeneID:20403"
                     /db_xref="MGI:MGI:1338049"
                     /translation="MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQ
                     ARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPI
                     MKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLV
                     PSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSS
                     SSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQS
                     NLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRG
                     GKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQR
                     EAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREA
                     AKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISG
                     RLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERI
                     KNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKEL
                     RESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESI
                     RKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFS
                     SGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLS
                     ATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLK
                     AQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQ
                     RGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVT
                     QKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGT
                     EQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFH
                     AVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTT
                     DSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESG
                     FLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAY
                     IRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPL
                     LIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGL
                     AEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHE
                     KLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERT
                     AWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSN
                     PYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLG
                     RTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL"
     exon            289..381
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            382..445
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            446..609
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            610..816
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            817..913
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1053
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1117
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1255
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1341
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1604
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1754
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1895
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..1983
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2123
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2260
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2397
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2495
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            2496..2671
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2801
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..3007
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3008..3093
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3094..3260
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3261..3306
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3307..3428
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3429..3525
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3526..3717
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3718..3839
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3840..3946
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            3947..4130
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            4131..4229
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            4230..4397
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            4398..4613
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            4614..4735
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            4736..4818
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            4819..4902
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            4903..5076
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            5077..5989
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5963..5968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="hexamer: AATAAA"
     polyA_site      5989
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="major polyA site"
ORIGIN      
        1 ttcctgcgga ggccgcggcc gccatgttgt tgtggggctg aggcggcgcc ggctgagagc
       61 ccagcagccg tgacaggctg cgcaacaggt tcgctgcggc cggcctgacg actgacccgg
      121 cggcggcggc cgcggcacgg cagggtcttc ccggagcttg gccgcgccca cgcgccggtg
      181 tcgaggagcg cgcggggtcg cgccgggacg tgcgcgaggc gccagatggc tgagagctgc
      241 aagaagaagt caggatcatg atggctcagt ttcccacagc gatgaatgga gggccaaata
      301 tgtgggctat tacatctgaa gaacgtacta agcatgataa acagtttgat aacctcaaac
      361 cttcaggagg ttacataaca ggtgatcaag cccgtacttt tttcctacag tcaggtctgc
      421 cggccccggt tttagctgaa atatgggcct tatcagatct gaacaaggat gggaagatgg
      481 accagcaaga gttctctata gctatgaaac tcatcaagtt aaagttgcag ggccaacagc
      541 tgcctgtagt cctccctcct atcatgaaac aaccccctat gttctctcca ctaatctctg
      601 ctcgttttgg gatgggaagc atgcccaatc tgtccattca tcagccattg cctccagttg
      661 cacctatagc aacacccttg tcttctgcta cttcagggac cagtattcct cccctaatga
      721 tgcctgctcc cctagtgcct tctgttagta catcctcatt accaaatgga actgccagtc
      781 tcattcagcc tttatccatt ccttattctt cttcaacatt gcctcatgca tcatcttaca
      841 gcctgatgat gggaggattt ggtggtgcta gtatccagaa ggcccagtct ctgattgatt
      901 taggatctag tagctcaact tcctcaactg cttccctctc agggaactca cctaagacag
      961 ggacctcaga gtgggcagtt cctcagcctt caagattaaa gtatcggcaa aaatttaata
     1021 gtctagacaa aggcatgagc ggatacctct caggttttca agctagaaat gcccttcttc
     1081 agtcaaatct ctctcaaact cagctagcta ctatttggac tctggctgac atcgatggtg
     1141 acggacagtt gaaagctgaa gaatttattc tggcgatgca cctcactgac atggccaaag
     1201 ctggacagcc actaccactg acgttgcctc ccgagcttgt ccctccatct ttcagagggg
     1261 gaaagcaagt tgattctgtt aatggaactc tgccttcata tcagaaaaca caagaagaag
     1321 agcctcagaa gaaactgcca gttacttttg aggacaaacg gaaagccaac tatgaacgag
     1381 gaaacatgga gctggagaag cgacgccaag tgttgatgga gcagcagcag agggaggctg
     1441 aacgcaaagc ccagaaagag aaggaagagt gggagcggaa acagagagaa ctgcaagagc
     1501 aagaatggaa gaagcagctg gagttggaga aacgcttgga gaaacagaga gagctggaga
     1561 gacagcggga ggaagagagg agaaaggaga tagaaagacg agaggcagca aaacaggagc
     1621 ttgagagaca acgccgttta gaatgggaaa gactccgtcg gcaggagctg ctcagtcaga
     1681 agaccaggga acaagaagac attgtcaggc tgagctccag aaagaaaagt ctccacctgg
     1741 aactggaagc agtgaatgga aaacatcagc agatctcagg cagactacaa gatgtccaaa
     1801 tcagaaagca aacacaaaag actgagctag aagttttgga taaacagtgt gacctggaaa
     1861 ttatggaaat caaacaactt caacaagagc ttaaggaata tcaaaataag cttatctatc
     1921 tggtccctga gaagcagcta ttaaacgaaa gaattaaaaa catgcagctc agtaacacac
     1981 ctgattcagg gatcagttta cttcataaaa agtcatcaga aaaggaagaa ttatgccaaa
     2041 gacttaaaga acaattagat gctcttgaaa aagaaactgc atctaagctc tcagaaatgg
     2101 attcatttaa caatcagctg aaggaactca gagaaagcta taatacacag cagttagccc
     2161 ttgaacaact tcataaaatc aaacgtgaca aattgaagga aatcgaaaga aaaagattag
     2221 agcaaattca aaaaaagaaa ctagaagatg aggctgcaag gaaagcaaag caaggaaaag
     2281 aaaacttgtg gagagaaagt attagaaagg aagaagagga aaagcaaaaa cgactccagg
     2341 aagaaaagtc acaggacaaa actcaagaag aggaacgaaa agctgaggca aaacaaagtg
     2401 agacagccag tgctttggtg aattacagag cactgtaccc ttttgaagca agaaaccatg
     2461 atgagatgag ttttagttct ggggatataa ttcaggttga tgaaaaaact gtaggagagc
     2521 ctggttggct ttatggtagt tttcagggaa agtttggctg gttcccgtgc aactatgtag
     2581 aaaaagtgct gtcaagtgaa aaagctctgt ctcctaagaa ggccttactt cctcctacag
     2641 tgtctctctc tgctacctca acttcttccc agccaccagc atcagtgact gattatcaca
     2701 atgtatcctt ctcaaacctt actgttaata caacatggca gcagaagtca gcttttaccc
     2761 gcactgtgtc ccctggatct gtgtccccca ttcacggaca ggggcaggct gtagaaaacc
     2821 tgaaagccca ggccctttgt tcctggacgg caaagaagga gaaccacctg aacttctcaa
     2881 agcacgacgt catcactgtc ctggagcagc aggaaaactg gtggtttggg gaggtgcacg
     2941 gaggaagagg atggttcccc aagtcttatg tcaagctcat tcctgggaat gaagtacagc
     3001 gaggagagcc agaagctttg tatgcagctg tgactaagaa acctacctcc acagcctatc
     3061 cagttacctc cacagcctat ccagttggag aagactacat tgcactttat tcatactcaa
     3121 gtgtagagcc cggggatttg actttcactg aaggtgaaga aattctagtg acccagaaag
     3181 atggagagtg gtggacagga agtattggag agagaactgg aatcttcccg tccaactacg
     3241 tcagaccaaa ggatcaagag aattttggga atgctagcaa atctggagca tcaaacaaaa
     3301 aacccgagat cgctcaagta acttcagcat atgctgcttc agggactgag cagctcagcc
     3361 ttgcgccagg acagttaata ttaatcttaa agaaaaacac aagcgggtgg tggcaaggag
     3421 agctacaggc cagagggaag aaacgacaga agggatggtt tcctgccagc catgtaaagc
     3481 tgctaggtcc aagcagtgaa agaaccatgc ctacttttca cgctgtatgt caagtgattg
     3541 ctatgtatga ctacatggcg aataacgaag atgagctcaa tttctccaaa ggacagctga
     3601 ttaatgttat gaacaaagat gaccctgact ggtggcaagg agaaaccaat ggtctgactg
     3661 gtctctttcc ttcaaactat gttaagatga caacagactc agatccaagt caacagtggt
     3721 gtgctgacct ccaagccctg gacacaatgc agcctacgga gaggaagcga cagggctaca
     3781 ttcacgagct cattcagaca gaggagcggt acatggacga cctgcagctg gtcatcgagg
     3841 tcttccagaa acggatggct gagtcaggct tcctcactga agcagacatg gctctgatct
     3901 ttgtgaactg gaaagagctc atcatgtcca acacgaagct gctgagggcc ttgcgggtga
     3961 ggaagaagac tgggggtgag aagatgccag ttcagatgat tggagacatc ctggcggcag
     4021 agctgtccca catgcaggcc tacatccgct tctgcagctg tcagcttaat ggggcaaccc
     4081 tgttacagca gaagacagac gaggacacgg acttcaagga atttctaaag aagttggcat
     4141 cagacccacg atgcaaaggg atgcccctct ccagcttcct gctgaagccc atgcagagga
     4201 tcactcgcta cccgctgctc atccgaagta tcctggagaa cactccacag agtcatgttg
     4261 accactcctc cctgaagctg gccctagaac gtgctgagga gctgtgctct caggtgaacg
     4321 agggagtccg ggagaaggaa aattcagacc ggctggagtg gatccaggca cacgtgcagt
     4381 gcgaaggctt ggcagagcaa cttattttca actccctcac caactgcctg ggcccccgga
     4441 agcttctgca cagcgggaag ctgtacaaga ccaagagcaa taaggagctg cacgccttcc
     4501 tcttcaacga cttcctgctg ctcacctacc tggtcaggca gtttgccgcc gcctctggcc
     4561 acgagaagct cttcaactcc aagtccagtg ctcagttccg gatgtacaaa acgcccattt
     4621 tcctgaatga agtgttggtg aaacttccca cagacccttc cagcgatgag cccgtcttcc
     4681 acatttccca cattgatcgt gtgtacacac tccgaacaga caacatcaac gagaggacgg
     4741 cctgggtcca gaagatcaag ggtgcctcag agcagtacat cgacactgag aagaagaaac
     4801 gggaaaaggc ttaccaagcc cgttctcaaa agacttcagg tattgggcgt ctgatggtgc
     4861 atgtcattga agctacagaa ttaaaagcct gcaaaccaaa cgggaaaagt aatccatact
     4921 gtgaagtcag catgggctcc caaagctata ccaccaggac cctgcaggac acactaaacc
     4981 ccaagtggaa cttcaactgc cagttcttca tcaaggatct ttaccaggac gttctgtgtc
     5041 tcactatgtt tgacagagac cagttttctc cagatgactt cttgggtcgt actgaagttc
     5101 cagtggcaaa aatccgaaca gaacaggaaa gcaaaggccc caccacccgc cgactactac
     5161 tgcacgaagt ccccactgga gaagtctggg tccgctttga cctgcaactt tttgaacaaa
     5221 aaactctcct ttgagggcct ggggaagcca gaaccagggg agctgcccac aaggctgggt
     5281 ctaaagacag attttgctct cccaggacag aggagcatca catggcttca tccatcaaac
     5341 agccacactc gctgggcctg tattttattg cacactaaat tgctagcaat ctatgcaaac
     5401 atgatctttt aaacaaacgc cacagcacag tgccttgtac tagtgttaac ctgttcagct
     5461 gtgttagatg ccagggtttc cattttcagg gctataaaag tattatgtgg aaatgaggca
     5521 tcagaccacc ggacgttacc acttggcaaa tctgtccact gtggagttgg tgatgttgga
     5581 accattccac actatgtgac ctctgctggg tcacacactc aggaggtgaa gggctgagat
     5641 gaaatgctgc agccttgggg cttgtgcagc ctgatactga aatagcatcc acttgtgcac
     5701 tgaataaata gaaacttgat cgttttattc tgactagata ttatcattct ctgctaagac
     5761 aatatagttt gaaatattat agtttgaata taaggaggaa agcttgatgt actttaaata
     5821 tactgtgaac tctaataatg tggggatatt tttcaacttt aattttctta agtataaatt
     5881 atttatgtaa attctttgtt ttgcatattt catagaacat gcatctttaa gctttatcat
     5941 tgccaacaat gtacagaaag agaataaaag tataagttta tgaatgtaaa aaaaaaaaaa
     6001 a
//
