LOCUS       NM_001198969            2350 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus intersectin 2 (Itsn2), transcript variant 3, mRNA.
ACCESSION   NM_001198969
VERSION     NM_001198969.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Vollweiter,D., Shergill,J.K., Hilse,A., Kochlamazashvili,G.,
            Koch,S.P., Mueller,S., Boehm-Sturm,P., Haucke,V. and Maritzen,T.
  TITLE     Intersectin deficiency impairs cortico-striatal neurotransmission
            and causes obsessive-compulsive behaviors in mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (35), e2304323120 (2023)
   PUBMED   37603735
REFERENCE   3  (bases 1 to 2350)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2350)
  AUTHORS   Locard-Paulet,M., Voisinne,G., Froment,C., Goncalves Menoita,M.,
            Ounoughene,Y., Girard,L., Gregoire,C., Mori,D., Martinez,M.,
            Luche,H., Garin,J., Malissen,M., Burlet-Schiltz,O., Malissen,B.,
            Gonzalez de Peredo,A. and Roncagalli,R.
  TITLE     LymphoAtlas: a dynamic and integrated phosphoproteomic resource of
            TCR signaling in primary T cells reveals ITSN2 as a regulator of
            effector functions
  JOURNAL   Mol Syst Biol 16 (7), e9524 (2020)
   PUBMED   32618424
  REMARK    GeneRIF: LymphoAtlas: a dynamic and integrated phosphoproteomic
            resource of TCR signaling in primary T cells reveals ITSN2 as a
            regulator of effector functions.
REFERENCE   5  (bases 1 to 2350)
  AUTHORS   Nishino,T., Tamada,K., Maeda,A., Abe,T., Kiyonari,H., Funahashi,Y.,
            Kaibuchi,K., Takumi,T. and Konishi,H.
  TITLE     Behavioral analysis in mice deficient for GAREM2 (Grb2-associated
            regulator of Erk/MAPK subtype2) that is a subtype of highly
            expressing in the brain
  JOURNAL   Mol Brain 12 (1), 94 (2019)
   PUBMED   31718706
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2350)
  AUTHORS   Choi,Y.S. and Sinha,S.
  TITLE     Determination of the consensus DNA-binding sequence and a
            transcriptional activation domain for ESE-2
  JOURNAL   Biochem J 398 (3), 497-507 (2006)
   PUBMED   16704374
  REMARK    GeneRIF: The isolation and characterization of ESE2 is reported.
REFERENCE   7  (bases 1 to 2350)
  AUTHORS   Rush,J., Moritz,A., Lee,K.A., Guo,A., Goss,V.L., Spek,E.J.,
            Zhang,H., Zha,X.M., Polakiewicz,R.D. and Comb,M.J.
  TITLE     Immunoaffinity profiling of tyrosine phosphorylation in cancer
            cells
  JOURNAL   Nat Biotechnol 23 (1), 94-101 (2005)
   PUBMED   15592455
REFERENCE   8  (bases 1 to 2350)
  AUTHORS   Yoshizawa,M., Sone,M., Matsuo,N., Nagase,T., Ohara,O., Nabeshima,Y.
            and Hoshino,M.
  TITLE     Dynamic and coordinated expression profile of dbl-family guanine
            nucleotide exchange factors in the developing mouse brain
  JOURNAL   Gene Expr Patterns 3 (3), 375-381 (2003)
   PUBMED   12799088
REFERENCE   9  (bases 1 to 2350)
  AUTHORS   Okamoto,M., Schoch,S. and Sudhof,T.C.
  TITLE     EHSH1/intersectin, a protein that contains EH and SH3 domains and
            binds to dynamin and SNAP-25. A protein connection between
            exocytosis and endocytosis?
  JOURNAL   J Biol Chem 274 (26), 18446-18454 (1999)
   PUBMED   10373452
REFERENCE   10 (bases 1 to 2350)
  AUTHORS   Sengar,A.S., Wang,W., Bishay,J., Cohen,S. and Egan,S.E.
  TITLE     The EH and SH3 domain Ese proteins regulate endocytosis by linking
            to dynamin and Eps15
  JOURNAL   EMBO J 18 (5), 1159-1171 (1999)
   PUBMED   10064583
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC241534.2, BC138262.1 and AK031390.1.
            
            On Mar 7, 2012 this sequence version replaced NM_001198969.1.
            
            Transcript Variant: This variant (3) lacks multiple 3' exons and
            has a novel 3' terminus, compared to variant 1. It encodes an
            isoform (3) with a shorter and distinct C-terminus, compared to
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK034367.1, ERR3363660.576212.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-195               AC241534.2         204244-204438
            196-1232            BC138262.1         173-1209
            1233-2350           AK031390.1         1-1118
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 2.09 cM"
     gene            1..2350
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="intersectin 2"
                     /db_xref="GeneID:20403"
                     /db_xref="MGI:MGI:1338049"
     exon            1..225
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            226..288
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     CDS             258..2216
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="isoform 3 is encoded by transcript variant 3; EH
                     and SH3 domains protein 2; SH3 domain-containing protein
                     1B; EH domain and SH3 domain regulator of endocytosis 2;
                     SH3 domain protein 1B; Eh domain, SH3 domain regulator of
                     endocytosis 2"
                     /codon_start=1
                     /product="intersectin-2 isoform 3"
                     /protein_id="NP_001185898.1"
                     /db_xref="GeneID:20403"
                     /db_xref="MGI:MGI:1338049"
                     /translation="MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQ
                     ARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPI
                     MKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLV
                     PSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSS
                     SSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQS
                     NLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRG
                     GKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQR
                     EAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREA
                     AKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISG
                     RLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERI
                     KNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCG
                     SMDDCVLQCLLSLLSCLNNLFLLLKVIF"
     exon            289..381
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            382..445
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            446..609
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            610..816
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            817..913
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1053
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1117
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1255
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1341
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1604
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1754
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1895
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..1983
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2123
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2350
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2317..2322
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="hexamer: AATAAA"
     polyA_site      2349
                     /gene="Itsn2"
                     /gene_synonym="Ese2; mKIAA1256; Sh3d1B; Sh3p18"
                     /note="major polyA site"
ORIGIN      
        1 ttcctgcgga ggccgcggcc gccatgttgt tgtggggctg aggcggcgcc ggctgagagc
       61 ccagcagccg tgacaggctg cgcaacaggt tcgctgcggc cggcctgacg actgacccgg
      121 cggcggcggc cgcggcacgg cagggtcttc ccggagcttg gccgcgccca cgcgccggtg
      181 tcgaggagcg cgcggggtcg cgccgggacg tgcgcgaggc gccagatggc tgagagctgc
      241 aagaagaagt caggatcatg atggctcagt ttcccacagc gatgaatgga gggccaaata
      301 tgtgggctat tacatctgaa gaacgtacta agcatgataa acagtttgat aacctcaaac
      361 cttcaggagg ttacataaca ggtgatcaag cccgtacttt tttcctacag tcaggtctgc
      421 cggccccggt tttagctgaa atatgggcct tatcagatct gaacaaggat gggaagatgg
      481 accagcaaga gttctctata gctatgaaac tcatcaagtt aaagttgcag ggccaacagc
      541 tgcctgtagt cctccctcct atcatgaaac aaccccctat gttctctcca ctaatctctg
      601 ctcgttttgg gatgggaagc atgcccaatc tgtccattca tcagccattg cctccagttg
      661 cacctatagc aacacccttg tcttctgcta cttcagggac cagtattcct cccctaatga
      721 tgcctgctcc cctagtgcct tctgttagta catcctcatt accaaatgga actgccagtc
      781 tcattcagcc tttatccatt ccttattctt cttcaacatt gcctcatgca tcatcttaca
      841 gcctgatgat gggaggattt ggtggtgcta gtatccagaa ggcccagtct ctgattgatt
      901 taggatctag tagctcaact tcctcaactg cttccctctc agggaactca cctaagacag
      961 ggacctcaga gtgggcagtt cctcagcctt caagattaaa gtatcggcaa aaatttaata
     1021 gtctagacaa aggcatgagc ggatacctct caggttttca agctagaaat gcccttcttc
     1081 agtcaaatct ctctcaaact cagctagcta ctatttggac tctggctgac atcgatggtg
     1141 acggacagtt gaaagctgaa gaatttattc tggcgatgca cctcactgac atggccaaag
     1201 ctggacagcc actaccactg acgttgcctc ccgagcttgt ccctccatct ttcagagggg
     1261 gaaagcaagt tgattctgtt aatggaactc tgccttcata tcagaaaaca caagaagaag
     1321 agcctcagaa gaaactgcca gttacttttg aggacaaacg gaaagccaac tatgaacgag
     1381 gaaacatgga gctggagaag cgacgccaag tgttgatgga gcagcagcag agggaggctg
     1441 aacgcaaagc ccagaaagag aaggaagagt gggagcggaa acagagagaa ctgcaagagc
     1501 aagaatggaa gaagcagctg gagttggaga aacgcttgga gaaacagaga gagctggaga
     1561 gacagcggga ggaagagagg agaaaggaga tagaaagacg agaggcagca aaacaggagc
     1621 ttgagagaca acgccgttta gaatgggaaa gactccgtcg gcaggagctg ctcagtcaga
     1681 agaccaggga acaagaagac attgtcaggc tgagctccag aaagaaaagt ctccacctgg
     1741 aactggaagc agtgaatgga aaacatcagc agatctcagg cagactacaa gatgtccaaa
     1801 tcagaaagca aacacaaaag actgagctag aagttttgga taaacagtgt gacctggaaa
     1861 ttatggaaat caaacaactt caacaagagc ttaaggaata tcaaaataag cttatctatc
     1921 tggtccctga gaagcagcta ttaaacgaaa gaattaaaaa catgcagctc agtaacacac
     1981 ctgattcagg gatcagttta cttcataaaa agtcatcaga aaaggaagaa ttatgccaaa
     2041 gacttaaaga acaattagat gctcttgaaa aagaaactgc atctaagctc tcagaaatgg
     2101 attcatttaa caatcagctg aagtgtggga gtatggatga ctgtgtcctt cagtgccttt
     2161 tgtctctgct gagctgtctc aacaacctct tcctcttact taaggttatt ttctaatatc
     2221 tagcttcctc tttttaagtt actttactgt tttctttttt taaactgaac tattctttgc
     2281 ctttatctga ttatatttgt ttggaataac gtatacaata aaaccttttt ttccttctgt
     2341 ttgctctaat
//
