LOCUS       NM_010587              13976 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus intersectin 1 (SH3 domain protein 1A) (Itsn1),
            transcript variant 1, mRNA.
ACCESSION   NM_010587 XM_358886
VERSION     NM_010587.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 13976)
  AUTHORS   Vollweiter,D., Shergill,J.K., Hilse,A., Kochlamazashvili,G.,
            Koch,S.P., Mueller,S., Boehm-Sturm,P., Haucke,V. and Maritzen,T.
  TITLE     Intersectin deficiency impairs cortico-striatal neurotransmission
            and causes obsessive-compulsive behaviors in mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (35), e2304323120 (2023)
   PUBMED   37603735
REFERENCE   3  (bases 1 to 13976)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 13976)
  AUTHORS   Qin,S., Predescu,D., Carman,B., Patel,P., Chen,J., Kim,M., Lahm,T.,
            Geraci,M. and Predescu,S.A.
  TITLE     Up-Regulation of the Long Noncoding RNA X-Inactive-Specific
            Transcript and the Sex Bias in Pulmonary Arterial Hypertension
  JOURNAL   Am J Pathol 191 (6), 1135-1150 (2021)
   PUBMED   33836164
REFERENCE   5  (bases 1 to 13976)
  AUTHORS   Malakooti,N., Pritchard,M.A., Chen,F., Yu,Y., Sgambelloni,C.,
            Adlard,P.A. and Finkelstein,D.I.
  TITLE     The Long Isoform of Intersectin-1 Has a Role in Learning and Memory
  JOURNAL   Front Behav Neurosci 14, 24 (2020)
   PUBMED   32161523
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13976)
  AUTHORS   Reymond,A., Marigo,V., Yaylaoglu,M.B., Leoni,A., Ucla,C.,
            Scamuffa,N., Caccioppoli,C., Dermitzakis,E.T., Lyle,R., Banfi,S.,
            Eichele,G., Antonarakis,S.E. and Ballabio,A.
  TITLE     Human chromosome 21 gene expression atlas in the mouse
  JOURNAL   Nature 420 (6915), 582-586 (2002)
   PUBMED   12466854
REFERENCE   7  (bases 1 to 13976)
  AUTHORS   Jenna,S., Hussain,N.K., Danek,E.I., Triki,I., Wasiak,S.,
            McPherson,P.S. and Lamarche-Vane,N.
  TITLE     The activity of the GTPase-activating protein CdGAP is regulated by
            the endocytic protein intersectin
  JOURNAL   J Biol Chem 277 (8), 6366-6373 (2002)
   PUBMED   11744688
REFERENCE   8  (bases 1 to 13976)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 13976)
  AUTHORS   Okamoto,M., Schoch,S. and Sudhof,T.C.
  TITLE     EHSH1/intersectin, a protein that contains EH and SH3 domains and
            binds to dynamin and SNAP-25. A protein connection between
            exocytosis and endocytosis?
  JOURNAL   J Biol Chem 274 (26), 18446-18454 (1999)
   PUBMED   10373452
REFERENCE   10 (bases 1 to 13976)
  AUTHORS   Sengar,A.S., Wang,W., Bishay,J., Cohen,S. and Egan,S.E.
  TITLE     The EH and SH3 domain Ese proteins regulate endocytosis by linking
            to dynamin and Eps15
  JOURNAL   EMBO J 18 (5), 1159-1171 (1999)
   PUBMED   10064583
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC062938.1, AF132481.1, AK082606.1 and AC126053.3.
            
            On Mar 30, 2007 this sequence version replaced NM_010587.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence data because no single transcript
            from the same strain was available for the full length of the gene.
            The extent of this transcript is supported by transcript
            alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF132481.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN01164131, SAMN01164132 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2447              BC062938.1         1-2447
            2448-2855           AF132481.1         2187-2594
            2856-3901           BC062938.1         2855-3900
            3902-5244           AK082606.1         765-2107
            5245-5406           AF132481.1         4984-5145
            5407-13976          AC126053.3         126628-135197       c
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 53.28 cM"
     gene            1..13976
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /note="intersectin 1 (SH3 domain protein 1A)"
                     /db_xref="GeneID:16443"
                     /db_xref="MGI:MGI:1338069"
     exon            1..229
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            230..289
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            290..382
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            383..446
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            447..607
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            608..787
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            788..884
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            885..985
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1049
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1187
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1300
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1563
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1713
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1854
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1942
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2082
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2210
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2437
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            2438..2559
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            2560..2807
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2967
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            2968..3173
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            3174..3256
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            3257..3423
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3469
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            3470..3591
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            3592..3709
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            3710..3901
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            3902..4023
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            4024..4130
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            4131..4314
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            4315..4413
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            4414..4581
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            4582..4794
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            4795..4916
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            4917..4999
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            5000..5083
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            5084..5257
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     exon            5258..13976
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5720..5725
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /note="hexamer: TATAAA"
     polyA_site      5745
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /note="major polyA site"
     regulatory      13955..13960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /note="hexamer: ATTAAA"
     polyA_site      13973
                     /gene="Itsn1"
                     /gene_synonym="Ehsh1; Ese1; Itsn; Sh3p17"
                     /note="major polyA site"
ORIGIN      
        1 aggagggcga gaggagtgga gcggcgcggg gagggcgcgc agcttggttg ctccgtagta
       61 cggcggctcg caagggagca tcccgagcgg gctccgggac ggccgggagg caggcaggcg
      121 ggcgggcggg gatggtgtgc gcggctgcgg actcggcgtt cctccgcgcg gcgtgcgggc
      181 tgcactgatt tgtgtgaggg gcggccgcgc gcacccgccc ggagatgagg cgtcgatcag
      241 caaggtgaac gtaatagaac catggctcag tttcccacac ctttcggtgg tagcctggat
      301 gtctgggcca taactgtgga ggaaagggcc aagcatgacc agcagttcct tagcctgaag
      361 ccgatagcgg gatttattac tggtgatcaa gcgaggaact tttttttcca atctgggtta
      421 cctcagcctg tcttagcaca aatatgggcg ctagcggaca tgaataacga tggaaggatg
      481 gatcaagtgg aattttccat agccatgaag cttatcaaac tgaagctaca aggatatcag
      541 ctcccctcca cacttccccc tgtcatgaaa cagcaaccag tggctatttc cagtgcacca
      601 gcatttggta taggagggat tgctagcatg ccaccactca cagctgttgc tcctgtgcca
      661 atgggctcca ttccagttgt tggaatgtct ccacccttag tatcttctgt ccctccagca
      721 gcagtgcctc ccctggctaa cggggctcct cccgtcatac agcctctgcc tgcgtttgcg
      781 catcctgcag ccacattgcc aaagagttct tccttcagca gatctggtcc agggtcacaa
      841 ttaaacacta agttacagaa ggcacaatca ttcgatgtcg ccagcgcccc tccagcagca
      901 gaatgggctg tgcctcagtc atcaaggctg aaatacaggc agttattcaa cagccacgac
      961 aaaactatga gtggacactt aacaggtccc caggcaagaa ctattctcat gcaatcaagt
     1021 ttaccccagg ctcagctggc ttcaatatgg aatctttctg acattgatca agatggaaaa
     1081 ctcactgcag aagaatttat cctagctatg cacctaattg atgttgccat gtctggtcag
     1141 ccactgccgc ccgtcctgcc tccagaatac atccctcctt ccttcagaag agttcgctcc
     1201 ggcagtggga tgtccgtcat aagctcttct tctgtggatc agaggctgcc tgaggagccg
     1261 tcgtcagagg atgagcagca gccagagaag aaactgcctg tgacatttga agataagaag
     1321 cgggagaact tcgagcgagg cagtgtggag ctggagaagc gccgccaagc gctcttggag
     1381 cagcagcgca aagagcagga gcggttggct cagctggagc gcgccgagca ggagaggaaa
     1441 gagcgggagc gccaggagca ggagcgcaag cggcagctgg agctggagaa gcagctggag
     1501 aagcagcggg agctggagcg gcagcgagag gaggagagga ggaaggagat cgagaggcgc
     1561 gaggccgcaa aacgggaact ggaaaggcag cgacaacttg aatgggaacg gaaccggaga
     1621 caggaactcc tgaatcagag gaacaaggag caggagggca ccgtggtcct gaaggcaagg
     1681 aggaagactc tggagtttga gttagaagct ctgaatgaca aaaagcatca gctagaagga
     1741 aaacttcagg atatcaggtg tcgactggca acccagaggc aagaaattga gagcacgaac
     1801 aagtctagag agctaagaat tgctgaaatc acccacttac agcagcagtt gcaggaatct
     1861 cagcaaatgc ttggaagact tattccagag aaacagatac tcagtgacca gttaaaacaa
     1921 gtccagcaga acagtttgca tagagactcg cttcttaccc tcaaaagagc cttggaagca
     1981 aaggagctgg cccggcagca gctccgggag cagctggacg aggtggagag agagaccagg
     2041 tcaaagctgc aggagattga tgttttcaac aaccagctga aggaactgag agagatacat
     2101 agcaaacagc aactccagaa gcagaggtcc ctggaggcag cgcgactgaa gcagaaagag
     2161 caggagagga agagcctgga gttagagaag caaaaggaag acgctcagag acgagttcag
     2221 gaaagggaca agcaatggct ggagcatgtg cagcaggagg agcagccacg cccccggaaa
     2281 ccccacgagg aggacagact gaagagggaa gacagtgtca ggaagaagga ggcggaagag
     2341 agagccaagc cggaaatgca agacaagcag agtcggcttt tccatccgca tcaggagcca
     2401 gctaagctgg ccacccaggc accctggtct accacagaga aaggcccgct taccatttct
     2461 gcacaggaga gtgtaaaagt ggtatattac cgagcgctgt acccctttga atccagaagt
     2521 cacgatgaga tcaccatcca gccaggagat atagtcatgg tggatgaaag ccagactgga
     2581 gagccaggat ggcttggagg agagctgaaa gggaagacgg gatggttccc tgcaaactat
     2641 gcagaaaaga ttccagaaaa tgaggttccc actccagcca aaccagtgac cgatctgaca
     2701 tctgcccctg cccccaaact ggctctgcgt gagacccctg ctcctttgcc agtgacctct
     2761 tctgagccct ccacaacccc caacaactgg gcagacttca gttccacgtg gcccagcagc
     2821 tcaaacgaga agccagaaac ggacaactgg gatacatggg cggctcagcc ttctctgacc
     2881 gtacctagtg ctggccagtt acggcagaga tcagccttta ccccagccac agccactggc
     2941 tcctccccat ctcccgtcct gggccagggt gaaaaggtgg aagggctaca agcgcaagcc
     3001 ctgtatccct ggagagccaa aaaagacaac cacttaaatt ttaacaaaag tgacgtcatc
     3061 accgttctgg aacagcaaga catgtggtgg tttggagaag ttcaaggtca gaagggttgg
     3121 ttccccaagt cttacgtgaa actcatttca gggcccgtaa ggaaatccac aagcatcgat
     3181 actggcccta ctgaaagtcc tgctagtcta aagagagtgg cttccccggc cgccaagcca
     3241 gccattcccg gagaagagtt tattgccatg tacacatacg agagttctga gcaaggagat
     3301 ttaacctttc agcaagggga tgtgattgtg gttaccaaga aagatggtga ctggtggacg
     3361 ggaacggtgg gcgacaagtc cggagtcttc ccttctaact atgtgaggct taaagattca
     3421 gagggctctg gaactgctgg gaaaacaggg agtttaggaa aaaaacctga aattgcccag
     3481 gttattgctt cctacgctgc tactggtccc gaacaactca ccctggctcc tgggcagctg
     3541 attctgatcc ggaaaaagaa cccaggtgga tggtgggaag gagaactgca agctcgaggg
     3601 aaaaagcgcc agatagggtg gtttccagca aattatgtca aacttctaag ccccggaaca
     3661 agcaaaatca ccccaactga gctacccaag accgcagtgc agccagcagt gtgccaggtg
     3721 atcgggatgt acgattacac cgcccagaac gatgacgaac tagccttcag caaaggccag
     3781 atcatcaacg tcctcaacaa ggaggacccg gactggtgga aaggagaagt cagtgggcaa
     3841 gttgggctct tcccatccaa ttatgtaaag ctgaccacag acatggaccc cagccagcaa
     3901 tggtgctcag acctgcatct cttagatatg ctgaccccga ctgagaggaa gcggcaaggc
     3961 tacatccatg aactcattgt cacggaggag aactacgtga acgacttgca gctggtcaca
     4021 gagatctttc agaaacccct gacggagtct gagctgctga cagaaaaaga ggttgctatg
     4081 atttttgtta actggaagga gctgatcatg tgtaatatca aactgctgaa agcgctgaga
     4141 gtccgcaaga agatgtctgg ggagaagatg ccggtgaaga tgattggcga catcctgagc
     4201 gcccagctgc cgcacatgca gccttacatc cgcttctgca gctgccagct caatggggct
     4261 gccctcatcc agcagaagac ggacgaggct ccagacttca aggagttcgt caaaagactg
     4321 gcaatggacc ctcggtgcaa aggaatgcct ctgtccagct ttatactgaa gcctatgcag
     4381 cgtgtcacaa gatacccgct gatcattaaa aacatcctgg aaaacactcc cgagaaccat
     4441 ccagaccaca gccacctgaa gcatgccctg gaaaaggcgg aggagctatg ctcccaggtg
     4501 aacgagggag ttcgagagaa ggagaactca gaccggctgg agtggatcca agcccacgtg
     4561 cagtgtgaag gcctttctga gcaactggtg ttcaattcag tgaccaactg cttgggacca
     4621 cgcaagtttc tgcacagcgg gaagctctac aaggccaaga gcaacaaaga actgtatggc
     4681 ttcctcttca acgacttcct cctgctgacc caaatcacaa agcccttagg ctcttccggc
     4741 accgacaaag tcttcagccc caaatctaac cttcagtata aaatgtacaa aacgcccatt
     4801 ttcttaaatg aggttctagt aaaattgccc acggaccctt ctggagatga gcctatcttc
     4861 cacatttccc acatcgaccg ggtctacacc ctccgagcag agagcataaa tgagaggact
     4921 gcctgggtgc agaaaatcaa ggcggcgtct gagctctaca tagagacgga gaaaaagaag
     4981 cgagagaagg cgtacctggt ccgttcccag cgggcgaccg gtattggaag gttgatggtg
     5041 aacgtggtag aaggcattga gctgaagccc tgtcggtcac atggaaagag caacccgtac
     5101 tgtgaggtga ccatgggctc tcagtgccac atcaccaaga caatccagga cacgctaaac
     5161 cccaagtgga attctaactg ccagttcttc atcagagacc tggagcagga ggttctctgc
     5221 atcacagtgt ttgagaggga ccagttctcg cctgatgatt ttttgggtcg gacagagatc
     5281 cgagtggccg acatcaagaa agaccagggc tccaaggggc cggttacgaa gtgtctcctg
     5341 ctgcatgagg tccccacggg agagattgtg gtccgccttg acctgcagtt gtttgatgag
     5401 ccgtagcagc cctgcgatga tcgtagatga cttcctcctc aaggccccgt gcgggcgtgc
     5461 tgtctggtgg tcagcctcag agcaacgggg atgaagcaaa gacgaagccc ctcgaggctg
     5521 ctaggagtcg ttctcgacaa tcctgccctt caaaccatgt ctcattttat gaatccaaat
     5581 tctcttttcc tttgctctcc ctatggtctc atcatggctt ctagagtctc tgaaatctgt
     5641 gacctttaac taggttccat tgggagcctg gctccttccc tgggctggag gtgtgggtct
     5701 gattcctata aaatagattt ataaacacag tgactgtatt ttggagaact gtgtaggcct
     5761 cctatttctc tcctctacca gcccttcgta gactacttgg cctataatat cgtgtgtgtg
     5821 tgtccaatta tgacggaatt gtggcttcgg agcccagccc tccctgggag atggggccct
     5881 agattgaatg gctctggagc agacaccaac gtacttaacc ctctggagag ctgtcgctta
     5941 gaagacactc ccatgtgtcg ttagagctgt accattcccc tctagacttc cttagtgccc
     6001 atcagtggaa acacggtggt ggcatgctac agggtgattc cgttccgatt ctgggtgagc
     6061 ttggaggatc ctccaaaagc tcattatctg caaggttctg tcctgatgag ttaggtcctg
     6121 ccgtgggtca ctgcgggtca ctgggatgac cttggcttgg ttattgtcag agcatcctct
     6181 ttgggcttgg agaccctgga atgtttcatg agaagttctg tctggagacc agtcctgtct
     6241 gagcccattc tgtgcttgaa agacttgtga gaagtaacaa caacaatcct tagtggtcac
     6301 cgcaccccca aaccccacca caccaggaag tcatgcctta gatggatgaa gttcaaaaaa
     6361 gaaagaattc aagatagaat aggtagtgtg gagaaacgct aagaaaatcc attactggtt
     6421 tgagggaaag acctttggga acccgtgagc cccacatgtg gggtgggaaa atgggcgatg
     6481 ctcctgggca tacaacaacc tcttagtcat aggaacacac gcactttttg cgagaatcag
     6541 gagtgtatga ccataggcca ggcttttttg tctgagttct ttgggttttc tctcccttaa
     6601 atgctaaaag ataggctggt tatctagata acttgatttg ctagtgagaa gtgcaggctg
     6661 ttttggtccc tcgtttgcat ctccagtctg tggtcatggc ctggcctttg catgtctcag
     6721 gctcctgggc tcaggctttc ctgtgttggg tggcctggcc tttgcatgtc tcaggctcct
     6781 gggctcaggc tctcctgtgt tgggtggtgt tcctttttat ggtaggttct cagattttaa
     6841 ctaggtttct agaagtactt tgtaaatggt agtgtctcgg tgacagaata gccactctgc
     6901 tgaaatctga atgctccatg tgaccccaga aggtccctct ccgtgtgtgt gtgtgtgtgt
     6961 gtgtgtgtgt ttcaagtgat ttctcaaaac gtgtatgggg caaaggacaa cagtttacat
     7021 ttcatacttc caagaagcac ttagagttta tttattgaag atagtgttga cttttgtatc
     7081 aagaacaaca gacacaccta ttttttgaaa caagccaata aactcttcag gtagcagctt
     7141 tcagctgaac atgaggtaga ctttaacttc gagcatgcgt tggcttacca ctttcccagc
     7201 agaaaacact atcaaaacac tacacattca tgtttctgtt gttgttaaga aactgtttta
     7261 aatgatacct ataactagat ctgtgaccca tggtttcttg gttctggaac caggggggct
     7321 ttgttcggca tgttgataaa ggacatgctc ccatactgtg aaacacagac attctgtgtg
     7381 caaatggcca gtatagggtc accattacaa tcccttcccc tcccccttcc aagttgaaca
     7441 tgaacatgat gttgaacatc agtgttcctg taggaaggat gaggcatgca tggtgacccc
     7501 ataggttctg ggttacctac tcatagcaag caccccatga ttgtagtatg tgaatgcagt
     7561 ctgtgagtct ttcccccagg tgaccctact agacacccct gggttgtcct gaggggtgac
     7621 agatctggat tggtgtcatt tgttgtgatt aattgtattt ctggatgagc tgggctgacc
     7681 ctcagaccca gctgtgactc ctggtttctg ttgagatgga gtcctattgt gacccatggg
     7741 tgacacacag agtctcaaac cttgattctc tcctccttga ctctactttt ttgaccagct
     7801 agccttcagt tcctacaaat ggtaggtcct tgccaacgag gaatctgagg ctcagatgtc
     7861 tgctttgtaa atgcttgccc aggctgcacc acctggcacc ttgatgccag ctgttcagat
     7921 accccctgaa ggcatccctc ttctatgggc cacactgcca gtacctccca cactctgacc
     7981 tcctctcact caggaaggca ctttcctgga gcctggctat gctctgaccc tcagtcaccc
     8041 ctgagaatta tcccgagagc tctgaggaca gaagcccctg actgggcttc cttcatgggc
     8101 cacttgatcc aaagggtcag tccttgtctc tggggaagcg tccatgtttt gtcacccagc
     8161 agatggatgg atagctttat ctcttgggac cagatttgct gtgtccacaa gagtttggag
     8221 acatatgtca atcattaagg tccagatctg ccctcgatgg aacagagagc cttctagatg
     8281 agagtcagca ggaatgctgg agaaactggg caagggtgct gggttcaatt ttgtaatgat
     8341 atgtctctgt cattggacag caaataagaa acttctctcc ctaaagaacc tttcttcctt
     8401 attttcagac aggacctgac tccatagccc aagatgtcct tgaacttagg gtcctcctgt
     8461 ctcagcctcc cgtgtgctgg aatttcaagt gtgtcccgct gggctcagct gtcttcattt
     8521 ttataaaagg tggatcaatg ggccgctcat actgcctgtt atcttcactc agtgccaacg
     8581 tgtgttctag acgtgtgtca cgaggtcaca ggcgtctgga aagggtgatc gcaggttggt
     8641 cttccagggt gggacagcag ctacctcccc aggagctgct gggcaggaga gagctgggca
     8701 ggagagagct ggtgaccata tacagtccag ggtgtcctag aatccattaa tcaaggacac
     8761 atggacgggc ccctctggct ttcatttgac aggtggagaa cctgagccag ctgagaccgc
     8821 agggaggatt tgcaccccca agggctttgt ttctagctgt tcggacctgg cctgattcct
     8881 aggaagaagc caaacctctg atggattctt ttcctctttt agaccctgtc agaatccaga
     8941 tgcacgagag ttagagacag ttcctcctcc tcttcctcct ccccctcctc cctctcctct
     9001 tcctcctccc cctcctccct ctcctcctct tcctcctctc cctccccctc ctcctcctct
     9061 tccctggatt ggccccatgg gcccaaattc ttgtttttct ttgtttcccc cttattctcc
     9121 aaagacacat tgaagctgac atttcaggga atgccttatt gggcaggcct acagaggctg
     9181 gcttgtatga gtggtgggaa cctcctcaga agagcccaat cttgctgact agtaattgac
     9241 agtaaggagt ccccaacatg ggtcctaaga tgccaccccc tcctcccctg tatctccccg
     9301 ctcctcccct gcacagtctc ctttaaaggg gggtcagcac ctgacctttt cccaactcca
     9361 ttgcttgcct acctgccaag ggaccctgtt ctgcagggac gtgggggtgt gagggtcacc
     9421 gggccaaggt aatgcaggag aaaaaaggag ggaaggaaaa aggaagagag aaagaaggaa
     9481 ggatggatgt aaggatagaa gggatggagg aaggagggag acagggcagg tggtcaaaga
     9541 gaacgttaaa gaggcttcct cgcaggttat gaaggtaagg gctcactcag tgccacgtac
     9601 tccccgtggg ggtttagggg accgcatctc tggagaagag actcgcaaac tgaagatggg
     9661 acttacccct gccctccttg tcatggagac ttagacctct agtgtagcag cccctagaac
     9721 catcacggct tcctttcttt tttatttctt ttttaaagat ttatttatta tacttaaaaa
     9781 acagacacac cagaagaggg tgtcaaatct cattacggat ggttgtgagc caccatgtgg
     9841 ttgctgggat ttgaactcag gatcttcaga agagcagtca gtgctcttaa ccactgagcc
     9901 atctctccag cccccaccat cacggctttc tgagtggcaa attcccagag gcccttgggc
     9961 atagcattct cttttgaatg actcctatta atgaacattg tcctttgaag ggctattcaa
    10021 ggaaataatg aagacttctt gttagtttgg acttgcaata aataactggc tggttgccac
    10081 agggactcct ctttccagtt ccctgtttcc tgatacctag aaagtagctg agctagagga
    10141 agagaaagtc ccggagaccg tttcctcaca ggacattgca cgaggtccct gtggtcccaa
    10201 ggatccacta atgtgtataa tgacttcgtg tctgtaagcg ccaggaaagg ttctgtcctt
    10261 gagaagcaca ggaaaataca acccgtgaga aagatggcca ggatctcagg ctctctaagt
    10321 tgagagcatt gaaactccta tgtcagcacc ctgggcagac tgcagtcagg aacagcaaca
    10381 cagtgagcaa tgctgacagg atgctgttcc ttcagggggg ctctatcctg tccaggccgc
    10441 ctgttggggg ctgggaggca gagaagggac ctcctttgtg gcttttaagc caacttgtag
    10501 atctaatttt aagtcacttt aataacataa aattcctgct cagtggctgg gtagatcgct
    10561 gacaaagtgc tagcccggca aacctgaggc cctgagtttg atccccagcc ctttcgtaaa
    10621 gaaaccagat tcagcatgcg tagctgtagt accagagctg gggatgtgga gacaggggga
    10681 ttgttggact tgtttcttgg tgccgtggct cacaggggtt gggactgggg atgtcttgaa
    10741 ggataaggta cagaaaactc agtacggaaa cattagtgtt tatctccacc aaacaactaa
    10801 ctcagttcta ctcagaccta actagttcct ttagtaaaag gagctaatag tttatatttt
    10861 ggcaaataat tctcctagca ctgtgtgtgg ctgtcctatt tcagggtgaa ttgcccctac
    10921 gctgtgtcag gtcctatgct atattttaat cggatctgtt ctgttttctg gagcagccta
    10981 gagtctcgct gttattacag tgatgagtat tttagtgttc gaagtccacc gaggagagtt
    11041 tcattcagaa gcgggcttat tccgtcagcc ttcagattgc ccagcgtccc tccgggattt
    11101 ctggggagct ggccagtgac caaggctcct ttttacgctg tggaaattcc tcccttcttc
    11161 ttttagaacc atcgtacccg tatcctgtgc gagagctgcg cctgtggttt tctgcctccc
    11221 cggctatact cttttgtctt tcttgtctta tctcctgttc tcggtgaaaa caagagtggg
    11281 gaccttagac aaataggagc caaaggcagc gtggtggctt gtgtggccat ttccgcactg
    11341 tggggctctt cctacctaga tggttctatt ggcagggtca aggtgctggc gccccctgct
    11401 gcatcccagc agcattgctc ttgtggaact tgctagtgag aggggtgggg gtagggtcag
    11461 ggctagaacc cgactgtagt gctccccgac tctcccagct gagccgtgag caaagaagga
    11521 aggtgtgagt gaacccagcg ctgggcacct actgaaatgg cgttcctgcc tgctggggtg
    11581 gacagagacc ctcctcagct tccctgcctt ttctgtgcct tcagagggga atgttgcaat
    11641 tcagttctcc acagaaaagg actggcttcc tgaagtaggc ctctgtccct gcgagacagg
    11701 gtagccctac ttggtaggat agcagggtgt ctacctgaag acttcgactt tggacgtttt
    11761 aagtggcaaa tggtttgatg cagcccttgg atgcccggag tgtgtctgtg agtgagtgtg
    11821 tatgtgtgta ccaagcattg ggggatggct gtgtgtccca ctgaaggaga caggagatgt
    11881 tttgctctac ccttccccaa gctgcattca ggaccagctg gtaatgtgtg gaccaagtgc
    11941 aaaaataaaa atggcctcct ccttgctcca aatgattagg gatttcagca ggggcagcag
    12001 agaactgagc tgagtttggg ccctgctgtt tggaggggct ccaacaccct ggggctggcc
    12061 ttgactacac tgtgaatgga gcagggaggg ccttgttatg tctttcttct ttggaaaggt
    12121 ctccctggca ggctgcgcgg agctgagatg gaaacacatc gtcccttctc cctctctccc
    12181 ccctccctcc cttgttttct tttcctttcc ttgcagtgct aattatcaaa gccagggcct
    12241 cgtgcattct tagaaagtga ggtgcttttc aaaactctac tcttttatct cttaaaagta
    12301 caaacagtgc agaataatta cagcgcccga acccccggct aacacctgga agcgccctgg
    12361 atggacgggg aatggtcaac ataggttgat ggtttctcaa gtctccgtcc agggtgtgtc
    12421 tctggatatc aagtggggcc tttgagtctt caaagttgaa ttttctgctt tgaaatgaaa
    12481 cctttcccta ttcaagggtt ggtctcccgg tttctggtgt cacagaggag attctaaact
    12541 actttaatgg agaagagcct agaagagcac agggtccctg tctgacatcg agaggtcctt
    12601 tgttccttta gatactgctt tctggagaga gagtgggcgg agccagatgc ctctctgccc
    12661 tagaggccaa gtgtctgaga gtttactcct gggaggcagg gctctcacgg gatagatcct
    12721 ccatgtgtct ttgggtgttc gcaaagaaga aaggctctgc tgtgtgtgta acctctgcat
    12781 tctggaagcc cacagctctg gggaggccag aagttaggaa gccgctcctc aaaggtgtgt
    12841 gtgtgtgtgt gtgtgtgtgt gtgtgttgct ttgttgtgca gcctgtgttg tgttggatgc
    12901 tccaagccct ttgaggctct gtttgccgtt tgcagatgtg gacccatgtc cacacatgca
    12961 gagcaataat ctttctcgag tttcccgatt ccactcctgt aatcccagcg ctccagagat
    13021 agaagcagga gaatcaggag tttaaggcca gtctgggcta cgtgagaccc tctttcaaaa
    13081 ctagaaacac agaaactgag aagggaaaac ccacagaagg ctcacctgcc tcagctttaa
    13141 atcaccttaa atgggtcatg atccctagag ggacctgacc actactctgc cagcatccat
    13201 tttaatgagt gaagattttg gatacccttc atcaattaac attctctaag cgttacttgc
    13261 tgaatattgc tgtcagatcg ttctctcaaa tgatgctcaa tggtaagaca tgcatttaac
    13321 tgagcatttg ttgtcaatgg agctgagagg tcagcctcag ctctggtcct tggcagccca
    13381 caagctaaag tctccttgcc cctccgtctg ccgcggtgca cacaggggcg caggctacca
    13441 ttcccctgtg ctgtactgtc ttcgctcttc tgctctgcaa cattccacac gtcgatggtg
    13501 aacataggtg aacatatctg tacagaggcg agaaacctcc gccaaatgtc ctgtgtctga
    13561 cagtcagctc acagctcctg ggtttctgta ttttgtgcat tttccaacaa aggaaaaaga
    13621 cggaaatcca ctacataatg tgaaaagatt gtgttctgta tgtggttcgt caggaaatct
    13681 ttgctgtatg tttttggcaa tgatgattat atgtgtatac agtatgtaag aggctatgct
    13741 gttcaatgtt gcataaactt aacagtctct agtctgaata tctaacttgt tattgtaaaa
    13801 tgtgatttct catcgagaga ttatttttct gtatgtatat gagagtattt taggaatcta
    13861 acttaagtgc ataaactata gaagaaactt aaaatcatat cccaggagta gaaatgcgtg
    13921 atcaggtcac ttgggttcaa cccatctaaa ttttattaaa aaacaaatcc aaagtg
//
