LOCUS       NM_010583               4274 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus IL2 inducible T cell kinase (Itk), transcript variant
            2, mRNA.
ACCESSION   NM_010583
VERSION     NM_010583.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Anannya,O., Huang,W. and August,A.
  TITLE     The kinase ITK controls a Ca2+-mediated switch that balances TH17
            and Treg cell differentiation
  JOURNAL   Sci Signal 17 (846), eadh2381 (2024)
   PUBMED   39042726
  REMARK    GeneRIF: The kinase ITK controls a Ca[2+]-mediated switch that
            balances TH17 and Treg cell differentiation.
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4274)
  AUTHORS   Algahtani,M.M., Alshehri,S., Alqarni,S.S., Ahmad,S.F.,
            Al-Harbi,N.O., Alqarni,S.A., Alfardan,A.S., Ibrahim,K.E.,
            Attia,S.M. and Nadeem,A.
  TITLE     Inhibition of ITK Signaling Causes Amelioration in
            Sepsis-Associated Neuroinflammation and Depression-like State in
            Mice
  JOURNAL   Int J Mol Sci 24 (9), 8101 (2023)
   PUBMED   37175808
  REMARK    GeneRIF: Inhibition of ITK Signaling Causes Amelioration in
            Sepsis-Associated Neuroinflammation and Depression-like State in
            Mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4274)
  AUTHORS   Elmore,J., Carter,C., Redko,A., Koylass,N., Bennett,A., Mead,M.,
            Ocasio-Rivera,M., Huang,W., Singh,A. and August,A.
  TITLE     ITK independent development of Th17 responses during
            hypersensitivity pneumonitis driven lung inflammation
  JOURNAL   Commun Biol 5 (1), 162 (2022)
   PUBMED   35210549
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4274)
  AUTHORS   Thelen,F., Wissmann,S., Ruef,N. and Stein,J.V.
  TITLE     The Tec Kinase Itk Integrates Naive T Cell Migration and In Vivo
            Homeostasis
  JOURNAL   Front Immunol 12, 716405 (2021)
   PUBMED   34566971
  REMARK    GeneRIF: The Tec Kinase Itk Integrates Naive T Cell Migration and
            In Vivo Homeostasis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4274)
  AUTHORS   August,A., Gibson,S., Kawakami,Y., Kawakami,T., Mills,G.B. and
            Dupont,B.
  TITLE     CD28 is associated with and induces the immediate tyrosine
            phosphorylation and activation of the Tec family kinase ITK/EMT in
            the human Jurkat leukemic T-cell line
  JOURNAL   Proc Natl Acad Sci U S A 91 (20), 9347-9351 (1994)
   PUBMED   7524075
REFERENCE   7  (bases 1 to 4274)
  AUTHORS   Yao,L., Kawakami,Y. and Kawakami,T.
  TITLE     The pleckstrin homology domain of Bruton tyrosine kinase interacts
            with protein kinase C
  JOURNAL   Proc Natl Acad Sci U S A 91 (19), 9175-9179 (1994)
   PUBMED   7522330
REFERENCE   8  (bases 1 to 4274)
  AUTHORS   Janis,E.M., Siliciano,J.D., Isaac,D.D., Griffin,C.A., Hawkins,A.L.,
            Kozak,C.A. and Desiderio,S.
  TITLE     Mapping of the gene for the tyrosine kinase Itk to a region of
            conserved synteny between mouse chromosome 11 and human chromosome
            5q
  JOURNAL   Genomics 23 (1), 269-271 (1994)
   PUBMED   7829087
REFERENCE   9  (bases 1 to 4274)
  AUTHORS   Siliciano,J.D., Morrow,T.A. and Desiderio,S.V.
  TITLE     itk, a T-cell-specific tyrosine kinase gene inducible by
            interleukin 2
  JOURNAL   Proc Natl Acad Sci U S A 89 (23), 11194-11198 (1992)
   PUBMED   1280821
REFERENCE   10 (bases 1 to 4274)
  AUTHORS   Bernards,A. and de la Monte,S.M.
  TITLE     The ltk receptor tyrosine kinase is expressed in pre-B lymphocytes
            and cerebral neurons and uses a non-AUG translational initiator
  JOURNAL   EMBO J 9 (7), 2279-2287 (1990)
   PUBMED   2357970
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB843988.1, D14042.1, AK153613.1 and BY589043.1.
            
            On Aug 13, 2013 this sequence version replaced NM_010583.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the central coding region, compared to variant 1.
            The encoded isoform (2) is shorter, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D14042.1, L05631.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                BB843988.1         1-60
            61-2230             D14042.1           1-2170
            2231-4265           AK153613.1         2187-4221
            4266-4274           BY589043.1         352-360
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 27.75 cM"
     gene            1..4274
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /note="IL2 inducible T cell kinase"
                     /db_xref="GeneID:16428"
                     /db_xref="MGI:MGI:96621"
     exon            1..243
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..1965
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /EC_number="2.7.10.2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     tyrosine-protein kinase ITK/TSK; interleukin-2-inducible T
                     cell kinase; kinase TLK; T-cell-specific kinase"
                     /codon_start=1
                     /product="tyrosine-protein kinase ITK/TSK isoform 2"
                     /protein_id="NP_034713.2"
                     /db_xref="CCDS:CCDS24575.2"
                     /db_xref="GeneID:16428"
                     /db_xref="MGI:MGI:96621"
                     /translation="MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDR
                     HGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDCESRQRW
                     VLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPT
                     PEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHE
                     GYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVS
                     VFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLR
                     YPVCSWRQKAPVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTI
                     QEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQR
                     GLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLD
                     DQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEV
                     VEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL"
     exon            244..348
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            349..430
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            431..559
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            560..600
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            601..752
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            753..818
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            819..873
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            874..956
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1087
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1162
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1334
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1551
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1616
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1735
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1893
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..4274
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4248..4253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /note="hexamer: TATAAA"
     polyA_site      4272
                     /gene="Itk"
                     /gene_synonym="Emt; Tcsk; Tsk"
                     /note="major polyA site"
ORIGIN      
        1 gactctctaa agatcaactg catcctgtct cagtctccgc tgctcctctc cttcagtcgc
       61 actgcggggt gatgcccaag gcgcatcact tttcagaact ggaccatgaa caacttcatc
      121 ctcctggaag aacagctgat caagaagtcc caacagaaga gaaggacttc tccctcgaat
      181 tttaaagttc gtttctttgt cttaacgaaa gccagcctgg cctactttga ggaccgccac
      241 gggaagaagc gcacgttgaa gggctccatt gaactctcca gaatcaagtg tgtggagatt
      301 gtcaagagtg acattagcat cccgtgccac tataaatacc cttttcaggt cgtgcatgac
      361 aactatctcc tgtatgtgtt tgctccagac tgtgagagtc ggcagcgctg ggtgctgacc
      421 cttaaagaag aaacgaggaa taacaacagc ctggtatcca agtatcaccc taatttctgg
      481 atggatgggc ggtggaggtg ctgctcccag ctggagaagc ctgctgtagg ctgtgctccc
      541 tacgacccat ccaagaatgc ttcaaagaag cctcttcctc ctactcctga agacaacagg
      601 cggtcatttc aggaacctga agaaaccctg gtcattgcct tgtacgacta ccaaaccaac
      661 gaccctcagg agctcgcact gcggtgtgat gaagagtact acctgctgga cagctccgag
      721 atccactggt ggagggttca agacaaaaat gggcatgaag gatatgcacc aagcagttac
      781 ctggtagaaa aatctccaaa taaccttgaa acctatgagt ggtacaataa aagcatcagc
      841 cgcgacaaag ctgaaaaact tcttttggac acaggtaaag aaggagcttt catggtccga
      901 gattccagga cacccgggac atacacagtc tctgttttca ccaaggccat cataagtgag
      961 aacccctgta taaaacatta tcacatcaaa gaaacaaatg acagccccaa gcgctactac
     1021 gtggctgaga agtatgtgtt tgactccatc cctctcctca tccagtatca ccagtacaat
     1081 ggaggaggtt tggtcactcg actccgctat ccagtttgct cctggagaca aaaagcccct
     1141 gtcacagcag ggctaagata tgggaagtgg gtgatccaac cctcagagct aacgttcgtg
     1201 caggagattg gcagcgggca gtttgggctg gtgcatctcg gctactggct caacaaggac
     1261 aaggtggcca tcaagaccat tcaggaaggg gcgatgtcag aagaagactt tatcgaggag
     1321 gcggaagtca tgatgaaact ctctcacccc aaactggtgc agctctatgg ggtgtgcctg
     1381 gagcaagccc ccatctgcct ggtgtttgag ttcatggagc acggctgcct gtcggattac
     1441 cttcgaagtc agcggggtct ctttgctgcg gagaccctgc tgggcatgtg cctggatgtg
     1501 tgtgagggca tggcctacct ggaaaaagct tgtgtcatcc acagagacct ggcagccaga
     1561 aactgtttgg tgggagaaaa ccaggtcatc aaggtgtccg actttgggat gacaagattt
     1621 gtccttgatg atcaatatac cagctccacg ggcaccaaat tcccagtgaa gtgggcatcc
     1681 ccagaggtgt tctcctttag tcgctatagc agcaagtcag atgtgtggtc gtttggtgta
     1741 ctgatgtggg aagtcttcag tgaaggcaaa atcccatacg aaaaccgaag caattccgag
     1801 gtcgtggaag atatcagcac tggctttcgg ttatacaagc cccgcctggc ctcctgtcat
     1861 gtctaccaga tcatgaatca ttgctggaaa gagaaaccag aagaccggcc acccttctcc
     1921 cagctgttaa gccagctggc tgaaatcgca gaagctgggc tttagcaggg actcattgac
     1981 cagacacagc cataaatcct gagtgaagga acaatgtcct ttttccagag cattaaaagc
     2041 tgccaccagt ccagaaaccc ccacaggtgc ctggaccatg gtgccagcac ctatgcagcc
     2101 acaactgcag catcatagaa aagggtggag ggccggccac tgagccaaca gctgaaccac
     2161 aactggagca gtatctgcgt gtccacccca tcccagcctc ttgtcgtatg ctgtactcaa
     2221 agctccattg cccactctcg ggagcatccc ctccccctct tagcaatcag gagaggcaga
     2281 cgtaggccac actcttcttt attattactt ttaagaataa ttcaagtaac ctttaatttg
     2341 gcccaacgag acattgtgcc cattttatgg atggaagagc gggaaagcat cattcttttt
     2401 ctaagaaatg caagaggtga ttaaaccagg gtgttctact acttcaaggc tgtcagccac
     2461 agcttcctgc ctcacatgag gcggagcgga tgcttgtgcc agaggccgga tgccataagg
     2521 tttcagagca cactgccacc tctctttgat ggggcacaga gagctccacc gggtggatag
     2581 ggcagggtga cacattcaga ccccacggca ggaccacagg atgtctgagg tcggtcagga
     2641 atgagagggc ctctgatggt ggaagtaaag agctgcaaaa gaagcctttg ggttgtccct
     2701 gacccagcgg ccctgtttca cggtgagtca tgtgtgtgac tggatctcct gttctgtgca
     2761 ggatgctgaa gcagtgacta cagaacggtc atcattccag cctctcagga gcggcatcca
     2821 ctcagcactg tcatgatcct tgtgactttg agactttggg ttctttcaag cctttacggg
     2881 ttcagcagtc ttaaggttgc catgtaaggc tctcaagagc ccagcaaagg aagaaggcgt
     2941 ttgctcttgc ccaaactatc aggaaggaaa gaggcaagca ctgggcggtg cttccaaaat
     3001 gcacaggatg tctcctactg attggcactc gcacaggtga catgtgtata tcctgcacac
     3061 aggtcatccc tggcgaatca cagttcatac caacccaacc acgaggtatt attcatgtct
     3121 tacagaaagt ggacttggtc ttttaaaagc tggatagctg ccccagtgac agaactggga
     3181 aggacggaga ttgagatgac acccgaatat aactccagtg tagcctctta gctcttctca
     3241 atgctgcctc ctactgaggt tgggccgctg agcgcataac acaggaaagt ctgggttaat
     3301 gggcacccca ccccacagcc aagttgctcc tactcagagc actggggggc ccagtgggac
     3361 cctccaaatt gaaataggct gttctctttc ttcaccctca cctggatctg agttcacaac
     3421 cctggagcca ctcaaaatta accctttcat tctcccaacc taccagagtc acattgcctg
     3481 aggccattta tactagagct tcaaaggtgc tttcaactag tctcctgcag actgtttcct
     3541 gtaattatcc cttcaaccta gagctggctc tcttgtgaga aggaggattc cctggtgcca
     3601 ggcacactgg attttcctgc ggtgttcaga caaagcccct agaaaaatga tcagagtcac
     3661 agatccctct tcctaagtca cgtggcattt aggctggctt gaggctcaaa tgcatcactt
     3721 ccaactgtaa tcactgtgtc cagataaaac tctgtcattc agaaaatgaa cttcctcaac
     3781 tggtccacaa tcatctctgt ttattctctt ttgggaaacc ttaattaaga gacgatttct
     3841 gggacatcca atctggaaga taaacactgg aaccgagaga agtctgtggt gtggtttagg
     3901 agactggccc ccatacggtg gtattggctc tcttaacctc acatgcacga aggctttgat
     3961 ggacgcgtgt gcactgctta ctccagtgtc ctgcttggga agatgggatt ctcttctgct
     4021 ggtttctgtc ttacattttt tttgtaaatg gtgccctgtg ttcacagaaa ggttttggtt
     4081 tgaaatgtat tttgtaggcc ttgcaattca taatgtttgg aactatgtat ttatgcctag
     4141 atcaggagag tatttattgc ttgtcttcat gataaaaaat actgcaggta tgtcttaccc
     4201 tctgtggggg ccacaagtca atcattgctt atggaaaaaa atatatatat aaagctacat
     4261 gttttacaag tata
//
