LOCUS       NM_018746               3164 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus inter alpha-trypsin inhibitor, heavy chain 4 (Itih4),
            transcript variant 1, mRNA.
ACCESSION   NM_018746
VERSION     NM_018746.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ravindran,A., Holappa,L., Niskanen,H., Skovorodkin,I., Kaisto,S.,
            Beter,M., Kiema,M., Selvarajan,I., Nurminen,V., Aavik,E.,
            Aherrahrou,R., Pasonen-Seppanen,S., Fortino,V., Laakkonen,J.P.,
            Yla-Herttuala,S., Vainio,S., Ord,T. and Kaikkonen,M.U.
  TITLE     Translatome profiling reveals Itih4 as a novel smooth muscle
            cell-specific gene in atherosclerosis
  JOURNAL   Cardiovasc Res 120 (8), 869-882 (2024)
   PUBMED   38289873
  REMARK    GeneRIF: Translatome profiling reveals Itih4 as a novel smooth
            muscle cell-specific gene in atherosclerosis.
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3164)
  AUTHORS   Iwai,T., Ohyama,A., Osada,A., Nishiyama,T., Shimizu,M., Miki,H.,
            Asashima,H., Kondo,Y., Tsuboi,H., Mizuno,S., Takahashi,S.,
            Ishigami,A. and Matsumoto,I.
  TITLE     Role of inter-alpha-trypsin inhibitor heavy chain 4 and its
            citrullinated form in experimental arthritis murine models
  JOURNAL   Clin Exp Immunol 215 (3), 302-312 (2024)
   PUBMED   38190323
  REMARK    GeneRIF: Role of inter-alpha-trypsin inhibitor heavy chain 4 and
            its citrullinated form in experimental arthritis murine models.
REFERENCE   4  (bases 1 to 3164)
  AUTHORS   Goulding,D.R., Nikolova,V.D., Mishra,L., Zhuo,L., Kimata,K.,
            McBride,S.J., Moy,S.S., Harry,G.J. and Garantziotis,S.
  TITLE     Inter-alpha-inhibitor deficiency in the mouse is associated with
            alterations in anxiety-like behavior, exploration and social
            approach
  JOURNAL   Genes Brain Behav 18 (1), e12505 (2019)
   PUBMED   29987918
REFERENCE   5  (bases 1 to 3164)
  AUTHORS   Shi,X., Ohta,Y., Liu,X., Shang,J., Morihara,R., Nakano,Y., Feng,T.,
            Huang,Y., Sato,K., Takemoto,M., Hishikawa,N., Yamashita,T. and
            Abe,K.
  TITLE     Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice Brain of a
            Novel Alzheimer's Disease Model
  JOURNAL   J Alzheimers Dis 68 (4), 1667-1675 (2019)
   PUBMED   30958362
  REMARK    GeneRIF: Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice
            Brain of a Novel Alzheimer's Disease Model.
REFERENCE   6  (bases 1 to 3164)
  AUTHORS   Bhanumathy,C.D., Tang,Y., Monga,S.P., Katuri,V., Cox,J.A.,
            Mishra,B. and Mishra,L.
  TITLE     Itih-4, a serine protease inhibitor regulated in
            interleukin-6-dependent liver formation: role in liver development
            and regeneration
  JOURNAL   Dev Dyn 223 (1), 59-69 (2002)
   PUBMED   11803570
  REMARK    GeneRIF: role in liver development and regeneration
REFERENCE   7  (bases 1 to 3164)
  AUTHORS   Weinstein,M., Monga,S.P., Liu,Y., Brodie,S.G., Tang,Y., Li,C.,
            Mishra,L. and Deng,C.X.
  TITLE     Smad proteins and hepatocyte growth factor control parallel
            regulatory pathways that converge on beta1-integrin to promote
            normal liver development
  JOURNAL   Mol Cell Biol 21 (15), 5122-5131 (2001)
   PUBMED   11438667
REFERENCE   8  (bases 1 to 3164)
  AUTHORS   Cai,T., Yu,P., Monga,S.P., Mishra,B. and Mishra,L.
  TITLE     Identification of mouse itih-4 encoding a glycoprotein with two
            EF-hand motifs from early embryonic liver
  JOURNAL   Biochim Biophys Acta 1398 (1), 32-37 (1998)
   PUBMED   9602042
REFERENCE   9  (bases 1 to 3164)
  AUTHORS   Jean,L., Smih,F., Olivier,E., Soury,E., Simon-Chazottes,D.,
            Guenet,J.L., Mattei,M.G. and Salier,J.P.
  TITLE     Comparative assignments of the genes of the inter-alpha-inhibitor
            family in human and mouse: ITIH4 is close to ITIH1 and ITIH3, on
            HSA 3 and MMU 14
  JOURNAL   Genomics 41 (1), 139-140 (1997)
   PUBMED   9126497
REFERENCE   10 (bases 1 to 3164)
  AUTHORS   Heller,D.L., Gianola,K.M. and Leinwand,L.A.
  TITLE     A highly conserved mouse gene with a propensity to form pseudogenes
            in mammals
  JOURNAL   Mol Cell Biol 8 (7), 2797-2803 (1988)
   PUBMED   3405219
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY788238.1, AK050270.1 and
            BB809402.1.
            
            On Jan 24, 2014 this sequence version replaced NM_018746.3.
            
            Summary: This gene encodes a member of the inter-alpha trypsin
            inhibitor (IaI) family of plasma serine protease inhibitors with
            diverse functions as anti-apoptotic and matrix stabilizing
            molecules during development. This gene is predominantly expressed
            in the liver and the encoded protein was found to be a plasma
            kallikrein-sensitive glycoprotein. This gene is located in a
            cluster of related inter alpha trypsin inhibitor genes on
            chromosome 14. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Oct
            2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050016.1, AK050270.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BY788238.1         2-27
            27-2933             AK050270.1         2-2908
            2934-3164           BB809402.1         146-376
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.09 cM"
     gene            1..3164
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /note="inter alpha-trypsin inhibitor, heavy chain 4"
                     /db_xref="GeneID:16427"
                     /db_xref="MGI:MGI:109536"
     exon            1..143
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..2882
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; ITI heavy chain H4; inter-alpha-inhibitor heavy
                     chain 4"
                     /codon_start=1
                     /product="inter alpha-trypsin inhibitor, heavy chain 4
                     isoform 1 precursor"
                     /protein_id="NP_061216.2"
                     /db_xref="CCDS:CCDS26901.1"
                     /db_xref="GeneID:16427"
                     /db_xref="MGI:MGI:109536"
                     /translation="MKSPAPAHMWNLVLFLPSLLAVLPTTTAEKNGIDIYSLTVDSRV
                     SSRFAHTVVTSRVVNRADAVQEATFQVELPRKAFITNFSMIIDGVTYPGVVKEKAEAQ
                     KQYSAAVGRGESAGIVKTTGRQTEKFEVSVNVAPGSKITFELIYQELLQRRLGMYELL
                     LKVRPQQLVKHLQMDIYIFEPQGISILETESTFMTPELANALTTSQNKTKAHIRFKPT
                     LSQQQKSQSEQDTVLNGDFIVRYDVNRSDSGGSIQIEEGYFVHHFAPENLPTMSKNVI
                     FVIDKSGSMSGKKIQQTREALVKILKDLSPQDQFNLIEFSGEANQWKQSLVQATEENL
                     NKAVNYASRIRAHGGTNINNAVLLAVELLDRSNQAELLPSKSVSLIILLTDGDPTVGE
                     TNPTIIQNNVREAINGQYSLFCLGFGFDVNYPFLEKMALDNGGLARRIYEDSDSALQL
                     QDFYHEVANPLLSSVAFEYPSDAVEEVTRYKFQHHFKGSEMVVAGKLQDQGPDVLLAK
                     VSGQMHMQNITFQTEASVAQQEKEFKSPKYIFHNFMERLWALLTIQQQLEQRISASGA
                     ELEALEAQVLNLSLKYNFVTPLTHMVVTKPEGQEQFQVAEKPVEVGDGMQRLPLAAQA
                     HPFRPPVRGSKLMTVLKGSRSQIPRLGDAVRASRQYIPPGFPGPPGPPGFPAPPGPPG
                     FPAPPGPPLASGSDFSLQPSYERMLSLPSVAAQYPADPHLVVTEKSKESTIPEESPNP
                     DHPQVPTITLPLPGSSVDQLCVDILHSEKPMKLFVDPSQGLEVTGKYENTGFSWLEVT
                     IQKPHLQVHATPERLVVTRGRKNTEYKWKKTLFSVLPGLKMTMNMMGLLQLSGPDKVT
                     IGLLSLDDPQRGLMLLLNDTQHFSNNVKGELGQFYRDIVWEPPVEPDNTKRTVKVQGV
                     DYLATRELKLSYQEGFPGAEISCWTVEI"
     exon            144..304
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            305..409
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            410..572
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            573..683
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            684..812
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            813..929
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1098
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1224
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1406
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1592
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1732
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1887
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..1917
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..1977
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2190
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2241
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2274
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2391
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            2392..2560
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2715
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2812
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     exon            2813..3164
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3004..3009
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /note="hexamer: AATAAA"
     polyA_site      3028
                     /gene="Itih4"
                     /gene_synonym="ITI-HC4; Itih-4; PK-120"
                     /note="major polyA site"
ORIGIN      
        1 ataacccacc tgctccaggg ctcagaagca gtctggcggg caactgaagg cagatgaaga
       61 gccctgcccc tgcccacatg tggaaccttg tgctgttctt gccttcactg ttggctgtgc
      121 ttccgaccac tactgccgag aagaatggca tcgatatcta cagcctcacg gtggactccc
      181 gggtctcttc ccgatttgcc catactgttg tcaccagccg ggtggtcaac agagccgatg
      241 ctgttcaaga agcgaccttc caagtagagc tacccaggaa agccttcatc accaactttt
      301 ccatgatcat cgatggcgtg acctacccag gggttgtcaa agagaaggcc gaagcccaga
      361 aacaatacag tgccgccgtg ggcaggggag agagtgctgg catcgtcaag accactggga
      421 ggcagacaga gaagtttgaa gtgtcagtca acgtggcccc tggttccaag attaccttcg
      481 aactcatata ccaggaactg ctccaaaggc gactgggaat gtatgagcta ctcctcaaag
      541 tgaggcctca gcagctggtg aagcaccttc agatggacat ctacatcttt gagcctcagg
      601 gtattagcat cctggagaca gagagcacct tcatgacccc ggagctggca aatgccctta
      661 ccacttcaca gaacaagacc aaggctcata tccggttcaa gccgacgctc tcccagcaac
      721 agaagtctca gagtgagcag gacacggtgc tgaatgggga cttcatcgtc cgctatgatg
      781 tcaaccggtc tgactctggg ggctccattc agattgagga aggctacttt gtgcaccact
      841 ttgctccaga gaaccttcct acaatgtcca agaatgtgat ctttgtcatt gataaaagcg
      901 gatctatgtc aggcaagaaa atccagcaga cccgagaagc cctagtcaag atcttgaaag
      961 acctcagccc ccaagaccag ttcaacctca ttgagttcag tggggaagca aaccaatgga
     1021 agcagtcact ggtgcaagcg acagaagaga atttgaacaa ggctgtaaac tatgcttcca
     1081 ggatccgggc tcacggaggg accaacatca ataatgcagt gctgttggct gtggagctgc
     1141 tggacagaag caaccaagct gagctactgc cctcgaagag cgtctccctt atcatcctgc
     1201 tcacggacgg tgaccccact gtgggagaaa ccaaccccac gattatccag aacaacgtgc
     1261 gggaagccat caatgggcag tatagcctct tctgcctggg gttcggcttt gatgtgaact
     1321 atcctttcct ggagaagatg gcactggaca atggtggcct ggccaggcgc atctatgagg
     1381 attcagactc tgcactgcag cttcaggatt tctaccacga agtagccaat ccactgctct
     1441 catcagtggc cttcgaatac cccagtgatg ctgtggagga agtcactcgg tacaagttcc
     1501 aacaccactt taagggctca gagatggtgg tggctgggaa gctccaggac cagggtcctg
     1561 atgtcctctt agccaaagtc agtgggcaga tgcacatgca gaacatcact ttccaaacgg
     1621 aggccagcgt agcccaacaa gagaaggagt ttaagagccc caagtacatc tttcacaact
     1681 ttatggagag actgtgggca ctgctgacta tacagcaaca gctggagcag aggatttcag
     1741 cgtcaggtgc cgaattagag gccctcgagg cccaagttct gaacttgtca ctcaagtaca
     1801 attttgtcac ccctctcacg cacatggtgg tcaccaaacc tgaaggtcaa gaacaattcc
     1861 aagttgctga gaagcctgtg gaagtcggtg atggcatgca gagactcccc ttagcagctc
     1921 aagcccaccc cttcaggcct cctgtcagag gatctaaact gatgaccgtg ctgaaaggaa
     1981 gcaggtccca gatacccaga ctcggtgatg ccgttagggc atctaggcaa tacattcctc
     2041 ccggattccc cggacctcct ggacctcccg gatttcctgc acctcctgga cctcctggat
     2101 ttcctgcacc ccctggacct cctcttgctt ctggctctga cttcagcctt cagccttcct
     2161 atgaaaggat gctaagcctg ccctccgttg cagcacaata tcctgctgac ccacatctgg
     2221 ttgtgacgga aaaaagtaaa gaaagcacca taccagagga atccccaaac ccagaccacc
     2281 cccaggttcc tactattacc ttgccgcttc cgggatccag tgtggaccag ctctgtgtgg
     2341 atatcttaca ttctgagaag cccatgaagc tgttcgtaga ccccagtcag ggtctggagg
     2401 tgactggtaa gtatgagaat actgggttct cgtggctcga agtgaccatc cagaagcctc
     2461 acctgcaggt ccatgcaacc cctgaacgac tggtggtgac acgaggcaga aaaaacactg
     2521 aatacaagtg gaagaagacg ctgttctctg tgttacctgg cttgaagatg accatgaata
     2581 tgatgggact cctacagctc agtggcccag acaaagtcac catcggcctc ctgtccctgg
     2641 atgaccctca gagaggacta atgctgcttt tgaatgacac ccagcacttc tccaacaacg
     2701 ttaaagggga gcttggtcag ttttaccggg acatcgtctg ggagccaccc gtcgagccag
     2761 ataatacaaa acggacagtc aaagttcaag gagttgacta cctggctacc agagagctca
     2821 agttgagtta ccaagaaggg ttcccaggag cagagatttc ctgctggaca gtggagatat
     2881 agaactgtta ggagcgccac tccctgccat gttgtcctcg tacgcaggca gatgacacct
     2941 tatgccaaca gggacgcctg tgaggccgag accttgatgg gaagaggatg ctcccttgtt
     3001 acaaataaag aagggcagtg tgaacccagg cagttgatgt catgattcca tgtggccagt
     3061 cctgtgggac ttagggtccc aacctgtata ttctgtctca aagaacatta aactcttctg
     3121 gaactggccc ttgacagcct gggaccaggg aaacttttca cacc
//
