LOCUS       NM_177290               8402 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus integrin beta 8 (Itgb8), mRNA.
ACCESSION   NM_177290 XM_484197 XM_901353 XM_901358 XM_906066 XM_919658
            XM_919665 XM_986358 XM_986389 XM_986421
VERSION     NM_177290.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Yang,J., Zhang,Y.N., Wang,R.X., Hao,C.Z., Qiu,Y., Chi,H.,
            Luan,W.S., Tang,H., Zhang,X.J., Sun,X., Sheps,J.A., Ling,V., Cao,M.
            and Wang,J.S.
  TITLE     ZFYVE19 deficiency: a ciliopathy involving failure of cell
            division, with cell death
  JOURNAL   J Med Genet 61 (8), 750-758 (2024)
   PUBMED   38816193
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 8402)
  AUTHORS   Cao,Y., Su,H., Zeng,J., Xie,Y., Liu,Z., Liu,F., Qiu,Y., Yi,F.,
            Lin,J., Hammes,H.P. and Zhang,C.
  TITLE     Integrin beta8 prevents pericyte-myofibroblast transition and renal
            fibrosis through inhibiting the TGF-beta1/TGFBR1/Smad3 pathway in
            diabetic kidney disease
  JOURNAL   Transl Res 265, 36-50 (2024)
   PUBMED   37931653
  REMARK    GeneRIF: Integrin beta8 prevents pericyte-myofibroblast transition
            and renal fibrosis through inhibiting the TGF-beta1/TGFBR1/Smad3
            pathway in diabetic kidney disease.
REFERENCE   4  (bases 1 to 8402)
  AUTHORS   Yin,Z., Rosenzweig,N., Kleemann,K.L., Zhang,X., Brandao,W.,
            Margeta,M.A., Schroeder,C., Sivanathan,K.N., Silveira,S.,
            Gauthier,C., Mallah,D., Pitts,K.M., Durao,A., Herron,S., Shorey,H.,
            Cheng,Y., Barry,J.L., Krishnan,R.K., Wakelin,S., Rhee,J., Yung,A.,
            Aronchik,M., Wang,C., Jain,N., Bao,X., Gerrits,E., Brouwer,N.,
            Deik,A., Tenen,D.G., Ikezu,T., Santander,N.G., McKinsey,G.L.,
            Baufeld,C., Sheppard,D., Krasemann,S., Nowarski,R., Eggen,B.J.L.,
            Clish,C., Tanzi,R.E., Madore,C., Arnold,T.D., Holtzman,D.M. and
            Butovsky,O.
  TITLE     APOE4 impairs the microglial response in Alzheimer's disease by
            inducing TGFbeta-mediated checkpoints
  JOURNAL   Nat Immunol 24 (11), 1839-1853 (2023)
   PUBMED   37749326
REFERENCE   5  (bases 1 to 8402)
  AUTHORS   Akagbosu,B., Tayyebi,Z., Shibu,G., Paucar Iza,Y.A., Deep,D.,
            Parisotto,Y.F., Fisher,L., Pasolli,H.A., Thevin,V., Elmentaite,R.,
            Knott,M., Hemmers,S., Jahn,L., Friedrich,C., Verter,J., Wang,Z.M.,
            van den Brink,M., Gasteiger,G., Grunewald,T.G.P., Marie,J.C.,
            Leslie,C., Rudensky,A.Y. and Brown,C.C.
  TITLE     Novel antigen-presenting cell imparts Treg-dependent tolerance to
            gut microbiota
  JOURNAL   Nature 610 (7933), 752-760 (2022)
   PUBMED   36070798
REFERENCE   6  (bases 1 to 8402)
  AUTHORS   McCarty,J.H., Lacy-Hulbert,A., Charest,A., Bronson,R.T.,
            Crowley,D., Housman,D., Savill,J., Roes,J. and Hynes,R.O.
  TITLE     Selective ablation of alphav integrins in the central nervous
            system leads to cerebral hemorrhage, seizures, axonal degeneration
            and premature death
  JOURNAL   Development 132 (1), 165-176 (2005)
   PUBMED   15576410
REFERENCE   7  (bases 1 to 8402)
  AUTHORS   Zhu,J., Motejlek,K., Wang,D., Zang,K., Schmidt,A. and
            Reichardt,L.F.
  TITLE     beta8 integrins are required for vascular morphogenesis in mouse
            embryos
  JOURNAL   Development 129 (12), 2891-2903 (2002)
   PUBMED   12050137
  REMARK    GeneRIF: integrin beta8 is required for vascular morphogenesis by
            providing proper cues for capillary growth in both yolk sac and
            embryonic brain (integrin beta8)
REFERENCE   8  (bases 1 to 8402)
  AUTHORS   Mu,D., Cambier,S., Fjellbirkeland,L., Baron,J.L., Munger,J.S.,
            Kawakatsu,H., Sheppard,D., Broaddus,V.C. and Nishimura,S.L.
  TITLE     The integrin alpha(v)beta8 mediates epithelial homeostasis through
            MT1-MMP-dependent activation of TGF-beta1
  JOURNAL   J Cell Biol 157 (3), 493-507 (2002)
   PUBMED   11970960
REFERENCE   9  (bases 1 to 8402)
  AUTHORS   Stepp,M.A.
  TITLE     Alpha9 and beta8 integrin expression correlates with the merger of
            the developing mouse eyelids
  JOURNAL   Dev Dyn 214 (3), 216-228 (1999)
   PUBMED   10090148
REFERENCE   10 (bases 1 to 8402)
  AUTHORS   Nishimura,S.L., Boylen,K.P., Einheber,S., Milner,T.A., Ramos,D.M.
            and Pytela,R.
  TITLE     Synaptic and glial localization of the integrin alphavbeta8 in
            mouse and rat brain
  JOURNAL   Brain Res 791 (1-2), 271-282 (1998)
   PUBMED   9593935
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140349.4 and AC142263.3.
            
            On Feb 25, 2022 this sequence version replaced NM_177290.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC125343.1, SRR1660815.282081.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1130              AC140349.4         102041-103170       c
            1131-1216           AC140349.4         72888-72973         c
            1217-1391           AC140349.4         66776-66950         c
            1392-1638           AC140349.4         56450-56696         c
            1639-1804           AC140349.4         54869-55034         c
            1805-1963           AC140349.4         54188-54346         c
            1964-2059           AC142263.3         178549-178644       c
            2060-2149           AC142263.3         174628-174717       c
            2150-2284           AC142263.3         170919-171053       c
            2285-2687           AC142263.3         168280-168682       c
            2688-2913           AC142263.3         165632-165857       c
            2914-3020           AC142263.3         164388-164494       c
            3021-3184           AC142263.3         160970-161133       c
            3185-8402           AC142263.3         155655-160872       c
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 63.48 cM"
     gene            1..8402
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /note="integrin beta 8"
                     /db_xref="GeneID:320910"
                     /db_xref="MGI:MGI:1338035"
     exon            1..1130
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     CDS             1004..3307
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /codon_start=1
                     /product="integrin beta-8 precursor"
                     /protein_id="NP_796264.2"
                     /db_xref="CCDS:CCDS36580.1"
                     /db_xref="GeneID:320910"
                     /db_xref="MGI:MGI:1338035"
                     /translation="MCGSALAFLTAALLSLHNCQRGPALVLGAAWVFSLVLGLGQSEH
                     NRCGSANVVSCARCLQLGPECGWCVQEDFVSGGSGSERCDTVSSLISKGCPVDSIEYL
                     SVHVVTSSENEINTQVTPGEVSVQLHPGAEANFMLKVRPLKKYPVDLYYLVDVSASMH
                     NNIEKLNSVGNDLSKKMALYSRDFRLGFGSYVDKTVSPYISIHPERIHNQCSDYNLDC
                     MPPHGYIHVLSLTENITEFEKAVHRQKISGNIDTPEGGFDAMLQAAVCESHIGWRKEA
                     KRLLLVMTDQTSHLALDSKLAGIVVPNDGNCHLKNNVYVKSTTMEHPSLGQLSEKLID
                     NNINVIFAVQGKQFHWYKDLLPLLPGAIAGEIESKAANLNNLVVEAYKKIISEVKVQL
                     ENQVHGVHFNITAICPDGARKPGISGCGNVTSNDEVLFNVTVVMKTCDIMGGKNYAII
                     KPIGFNETTKVHIHRSCSCQCENHRGLKGQCAEAAPDPKCPQCDDSRCHFDEDQFPSE
                     TCKPQEDQPVCSGRGVCICGKCLCHKTKLGRVYGQYCEKDDFSCPYLHGDVCAGHGEC
                     EGGRCQCFSGWEGDRCQCPSASAQHCVNSKGQVCSGRGTCVCGRCECTDPRSIGRLCE
                     HCPTCHLSCSENWNCLQCLHPHNLSQAALDQCKSSCAVMEQHRMDQTSECLSGPSYLR
                     IFFIIFIVTFLIGLLKVLIIRQVILQWNNNKIKSSSDYRMSASKKDKLILQSVCTRAV
                     TYRREKPEEIKMDISKLNAQEAFRCNF"
     exon            1131..1216
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1391
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1638
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1804
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1963
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2059
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2149
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2284
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2687
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            2688..2913
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            2914..3020
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3184
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..8402
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8377..8382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /note="hexamer: AATAAA"
     regulatory      8385..8390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /note="hexamer: CATAAA"
     polyA_site      8397
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
                     /note="major polyA site"
     polyA_site      8402
                     /gene="Itgb8"
                     /gene_synonym="4832412O06Rik; D630049N15"
ORIGIN      
        1 actgcgcttg ctggcgaccc aagcaaggag cactgtagcg tggggaggtt tcctagcaac
       61 tctcagctcg gagcacgccc agccacccga aagctgggag cccccacggg ggaccacaga
      121 actagatcac cccatccctg cccatcgcct gcctctcttc cctctcatcc cagaacgcat
      181 cagctgccag ggttctgggt ggccagacgc ctgagccgac agagtccgag aaaggacaag
      241 ggtacgcagc tccctcccag cgaggtgcat ggaacatccc cttctagccc agtaagcaac
      301 tgggatgcct tgttggttgc ccgcgagtgc agacaggagc ttctagggag ctcgcggagc
      361 tgcttcccca agctgctggc acccgtgtct cctctcccgc caatcggaac gctgccgcct
      421 gaacctctgc tagctgcgac taagtggagt tggcctccct gcccacctgt ggaagaagct
      481 agcaccgact gatgcgccag caactccccc acccccttgg ccttctagcc gtcccctccc
      541 actaaagcag catcaccctg tgaattacat tagggtggtt ttccccccag cctcaggctt
      601 tgtttgggtt tgattgtgtt tggctcttcg ctaagctgat ttatgcagca gagccccatc
      661 ggctggggag aaacaaaagc tcttttcttt gtctgggagc ggactgtgga gcccgtactt
      721 gcttcgcccc cgcgggccat cggctgtcca aggaggtgct ttttgttgag actgcaagcc
      781 ccgccgtgcg gagctgggag ggccttaagg gccctgagat gcagagagct gccctggacc
      841 gaatacctgt atcgcctact gagagcaact gggtcggcca acccggcctg tcgaaaaggt
      901 cctagggact cgcagcccac gggcctcaag gtgcgctctc aagtcgggag agcgcatcag
      961 gaaagcaact aggcagtagg caccgggcgg gctgctttgc attatgtgcg gctcggccct
     1021 ggcttttctg actgctgcac tgctctctct gcacaactgc cagcgaggtc cagccttggt
     1081 tcttggggca gcctgggtat tttcacttgt tctcggactg ggccaaagtg aacacaatag
     1141 atgtggctct gcaaatgtgg tctcctgtgc caggtgcctc cagctgggtc cggaatgcgg
     1201 atggtgtgtt caagaggatt ttgtttcagg tggatcagga agtgaacgtt gtgatactgt
     1261 ttccagtttg ataagcaaag gctgtccagt tgattcaata gaatacctgt ctgtgcatgt
     1321 tgtaacgtca agtgagaatg aaatcaatac ccaggtgaca ccgggagaag tctcagtcca
     1381 actgcatcca ggagctgaag ctaattttat gttaaaagtc cgtcctctga agaaataccc
     1441 cgtggatctt tattatctgg ttgatgtgtc agcatcaatg cacaataata tagaaaaatt
     1501 gaattccgtt ggaaatgatt tatctaaaaa aatggccctt tattcccgtg acttccgtct
     1561 tggttttggc tcatatgtgg ataaaactgt ctcgccatac attagtatcc acccggaaag
     1621 gatccacaat cagtgcagtg actacaattt agactgtatg cctccccatg gatacattca
     1681 tgtgctgtct ctgacagaaa acatcactga gtttgaaaaa gcagtccaca gacaaaagat
     1741 ctctggaaac atagacaccc ctgaaggagg ttttgatgcc atgcttcagg ctgccgtctg
     1801 tgagagtcat attggatggc gaaaagaagc taaaagattg ctgctggtga tgacagacca
     1861 gacatcacat cttgctcttg acagcaaatt ggcaggcata gtggtgccga atgacggaaa
     1921 ctgccatctc aaaaacaatg tctacgtcaa atcgacaacc atggaacatc cgtcattagg
     1981 ccaactttct gaaaagttaa tagacaacaa cataaatgtc atttttgcag ttcaaggaaa
     2041 gcagtttcat tggtacaagg accttctgcc ccttttgcct ggtgccattg ctggtgaaat
     2101 agaatccaaa gctgcaaatc tcaacaattt agtagtagaa gcctacaaga agattatctc
     2161 agaagtaaaa gtgcagctgg aaaaccaggt acacggtgtc cattttaaca tcactgccat
     2221 ctgtccagat ggggccagaa agccaggcat aagtgggtgt ggaaacgtga caagcaatga
     2281 cgaagttctt ttcaatgtaa ctgttgtgat gaaaacatgt gatatcatgg gaggaaaaaa
     2341 ctatgcaata atcaagccta ttggtttcaa tgaaaccact aaagtccaca tacacagaag
     2401 ctgcagttgc cagtgtgaga accacagagg actcaaagga cagtgtgcgg aagctgcccc
     2461 agaccccaag tgtccacagt gtgatgacag cagatgtcat tttgatgaag accagtttcc
     2521 ttctgaaact tgcaagccac aggaggatca acctgtctgc agtggccgag gtgtttgcat
     2581 ctgtgggaaa tgtttatgtc acaaaaccaa gctaggaaga gtgtatggcc agtactgtga
     2641 gaaggatgac ttctcctgtc cctatctcca tggggatgtg tgtgctgggc atggggagtg
     2701 tgaaggtggc agatgccagt gctttagtgg ctgggaagga gatcggtgtc agtgcccatc
     2761 agcctcagca cagcactgtg tcaactccaa gggccaagtt tgcagcggga gaggcacctg
     2821 cgtgtgtggc aggtgtgagt gtactgatcc cagaagcatt ggccgtctct gtgagcactg
     2881 cccaacctgc catctctcct gcagtgaaaa ctggaattgt ctgcagtgcc ttcaccctca
     2941 caatctgtct caggctgccc ttgatcagtg taaatcctcc tgtgctgtca tggaacagca
     3001 tcgcatggac caaacatcag aatgtttatc tggcccaagc tatctgcgaa tatttttcat
     3061 catattcata gtcacattct tgatcgggtt gcttaaagtt cttatcatta gacaagtgat
     3121 actacaatgg aataataata aaataaagtc ctcatcagat tatagaatgt ctgcttctaa
     3181 aaaggataaa ctgattctgc aaagtgtttg cacgagagct gtaacctacc gacgagaaaa
     3241 gcctgaggaa ataaaaatgg atatcagcaa actaaatgct caggaggcct tcaggtgcaa
     3301 cttctaaata agattaacaa acaaacaaaa actgttggaa gctggatggt tttacaattt
     3361 attctagcaa gtataatata aagtcacagg agaagacaaa ttatattgaa gagtgtgcca
     3421 tgaagactgg caagtgtctt cacaaccata tgactcactc agctgctgaa gttttcagtg
     3481 aactatgtgt cttactactg ttggagacta gtgttgtagc actttattgt aatatataac
     3541 ttatttagat cagcgtagaa tgtagatcat ctgggaaagc actgattaca ctttacaggt
     3601 acctgtcagc cctgagcttc acagacaaac acgaggctat ggagcaggtt gcagggtgtc
     3661 accaccacag actggtgatc cttctgtaca acctgctgat gaaaagcatc caggagtagt
     3721 tgaaagcagt caaccacacc cggcaattgg cattgtcgag ctgtgtacaa ccagctgagt
     3781 aagggttagg ctttcatttc agtttgggtg aacagatgaa ctttaatcta agcggtgttg
     3841 cttttgaagc tgtgcagttt tgtgcatgca cgggtctgtt ttcttctcct ttgaagctgc
     3901 gtggctttgt gcatgtacaa atctgctttc ttctcttttc caaaatggat tttgactcca
     3961 acatcttctc ctttttgcat ttaaattctg tttttcttcc tttggaataa gtttataatg
     4021 tcacaaatga ctggattaaa taagtgctaa gttactactg ccatagaacc tatatgacaa
     4081 tgtcacttta ctagaatact ggttttaaag ctgaatgtta ataagggtca ctgtaaggtg
     4141 tctaaggtca tctaagtcca tagcttcttt gtctgaagat agtttatata ttcttacctg
     4201 tcaaactgga tggattgttt cactctgtca ttgatctgac ctgtagcccg aaatccaaaa
     4261 ggtgtctaca aaactgcttc gagcagagta ccctcttttc tgagtgtgtt ctcttgattt
     4321 ttactccaag atgatgtcaa cagttttcaa atggaaagct atattattct tgccctcctg
     4381 tgttccctaa gggcattcct tgaatcccaa ccctactcat aagaattcac aaaggtataa
     4441 aattggacac atgagggtaa tatcctggaa acaagctaat ctcccctttt tgttagccag
     4501 gaaatggagg ctcagaaaat taaaacgatt ttacaaaaca cagacgcgga gaaagtgaca
     4561 ataccagaga agagctgcct tccaattaca ggacagcatt ggacaataat actctacctc
     4621 ttgaatctgt aaaacatcca tggcaaaatt taaaatacga tttattcaag tcttgaatat
     4681 ttttcttgag aatatttcaa tgcaaagtag aaaagggtac tgtgcataag cagcttctag
     4741 ttttccccat tagccacctt ctgataaggc tttggtgcag cccttccctc cacagtgact
     4801 acaagaacaa gcctcttgga gctggccatg ctttgatcca gaggctcgtg acttggattc
     4861 taaattctaa ctgtcctttg tgtagcaagt gtcttacctg tgaaaacaga ggaatctaaa
     4921 gagtgtctct cctcagtttg ctgctctcca gcacttgaaa gttattagat attagcatgt
     4981 cgatgaacac tagcctctat taggtattat actctggggg ctggaacgcc tgtaaattat
     5041 tctctagtca gaaaacatga tgtgtccctt agcagagcta tgacaatgct cttgacttga
     5101 gtgtttactc aagttgagat gggcattgtc aaaatactgt gactgcacct aaagaaactg
     5161 tgctcactaa agctgatgtg aaaatcctac tttgctgctc agaatagggt ccttttataa
     5221 atatacatat taatggatgc ttggcagtcc ctcctcccaa aggaagatat ccattaatta
     5281 aatgctagca actcaaaagt gagcagtgtt tatgaacaat gatttccaca tgaaatgagc
     5341 aaaagagcat aatcaaagag aagccttgaa aaaaaatgat ttgtacattt ttaggccctc
     5401 actagtttgc actaatttta agactgacca aggctgttgc catgggaaag atgaatttcc
     5461 ttgccaagaa ttcagtgctg gatgataata tgtacttaca tgtggaatgt cttgtagcct
     5521 ttctttctct tctttttgtt aacttaaaaa gctaattttt aaaagattgt agggcttata
     5581 ttttacttga ataattgttc tgtttaatag tttgtgagaa gagaattaat atttactagg
     5641 tagaattaat taacagtaag gggaaacagg gtattcttag attaaataat ttatgtattt
     5701 tagactattt tcaaatgcta tgatcacatg agttatttga gttgttgata gtaaatgcaa
     5761 ttaattgaat tttaaattgt tagcaaaatg aataacgagt gataattttt ttggcctgtc
     5821 agcattttgt tccctggtgt gtaattattc aaaactgtcc catatttcca ctacataagc
     5881 aactatatac aagttatttc ttctgagaca tttttttaca ctgcagtaaa catgtattta
     5941 taaacatttg atacaatttt ttaggtattt tctactattt attaagccat atccattctc
     6001 taagagacat gttcaattta acttagttta taaaaataac aaaaacaaac taggtatctc
     6061 tgggggcaaa gttttcaaaa ttgacaaatt aaaattgtgt atatatcaaa tgcaaaaccg
     6121 ccaaagaata aaattcaaat atcttttaat atttactaaa cctccattaa ggatattctt
     6181 ttatatatca acaaacatct gtgatacact gaaatcatta ttcttgctag attatgaatg
     6241 tttttctcag tagaattata tttcttctgg cacttggtag acgagcttat actcaatgac
     6301 tcttccttcc ttcccgtctc ctgtttgtcc cattcacatt tctctagaaa ttgcccataa
     6361 gcttcaagcc aataagccta attttgaaaa ataacagctt aacaatataa ctaagttctt
     6421 catttccaaa aaaaatctct tattaatgtc ggcactcatt tggacaaata cagcccttct
     6481 gaaaacaaag aattttaaat gttcacaatg ttgtgattta tgttttgtaa aggtagcagc
     6541 tcattaggag gttcaatact gtattctcct tgtttactct tgatacttag cttacagcta
     6601 tccttatgtc cttaggactg taaaaacttc attttatgta atgcaaacgt tttcaaggga
     6661 tagcactaat attccttcaa atcctttcct tttcatcttc cataatccct gcatggcaaa
     6721 ttagttgtgg ataattgatt agacatacct actgtgtgtc ttgctgattc tagaagacat
     6781 ttttttttca atttagcatc ccccaaattg ggacatctct tgaaattgtc atctgcagtg
     6841 tagtgtttaa aactgtgttg agataaatac tacagtaatt aaatattcat ggatacagtt
     6901 ctttccatat aaatgtttat gaattcgttt ttgatgacat gtgaagccag tttttaatac
     6961 agctgaaccg catactactc aatgcaaacc taactggcta ttagctcgga tcctttgaat
     7021 ctttgagtta caaaagctct ttcaggctcc catttatact gtctgctaac agcagtgatt
     7081 taagcaaact gaagctaata aaagtgtgga gtttcttgag ctttcattta aggatcttta
     7141 gcattcccat tcctagttaa gggattgttt aaaatgatca tctcactttt aaataagtta
     7201 actggaaaaa agattacatg aaaacatcat agttagattt cacagcactt tgtaagtttg
     7261 atgccagtta ccagagacta aaatgatgca accagctagt cttgaaaaga tttcctctga
     7321 gatctcgcaa ctttctcatt tctccttatc ctttactttc ttttgatgtt ttggttggct
     7381 caagtaactt ttcctctaca gcccatgtga tgactgttat aaaaaaaaaa ctgcagttaa
     7441 aatatagtac tttatgtgaa acaattgttc aactctttag gcagcttttg atcgaatatg
     7501 tgagagtaat aacactactc tgtctacctg caataccttc ctgtatagaa tgtatctttg
     7561 taagctaact ctttcaacca ggtgtctatt cttcacaacc aatgtcagta caactgtgta
     7621 actcatcagt ataggagaaa gaaaataaaa aaccacattg caattatcaa ggatagaaat
     7681 gtgatttctg tagagaactt tactgacttt atacatataa atgtgagtct gaaaacatgt
     7741 ctgcacaatg acaagtatgg tacacagatt tttattgaaa tgttgagcaa aactattttc
     7801 attctaaatt ataagtacca atttgcacat tataatatag aaatccattt tgtatatatt
     7861 taacatatgt ttaaggctgt tttacatcta ctaccttctt tatgaaggtt tgtaaatctt
     7921 tgcaaaagca attagacatg ttttaaaatc aataacagtc tttgtttgac ttaacaaata
     7981 atgcctttgt aagaaacatt taatatagaa tgtattttta aatcaagaca tttgaatatg
     8041 aaatagctca aagaaaaaca aattcttcat gaacacattt tatgttgttt ctggctttga
     8101 ttatataaaa ggtgaaattg ccttaaagac agaagcttgg taaatatgac aatttcacgt
     8161 actatactat gaatctcctt gcataactgg ttgactacta acttctcttt tatgtattta
     8221 ttaaaagctg acactgtaga ttgttgtgag atgtttattt ttctaacaga gcttataact
     8281 gttgtgacac agcatttaat tgatatacca gtctgtttaa atgtaggtgt taatctatat
     8341 aaaattctat gttttaaaat aaaactttaa agatctaata aaaacataaa gaatatataa
     8401 aa
//
