LOCUS       NM_008395               5187 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus itchy, E3 ubiquitin protein ligase (Itch), transcript
            variant 1, mRNA.
ACCESSION   NM_008395 XM_001005744 XM_192925 XM_895069 XM_902524 XM_902525
            XM_902526 XM_902527 XM_902529 XM_907054 XM_976340
VERSION     NM_008395.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Lim,K., Rutherford,E.N., Delpiano,L., He,P., Lin,W., Sun,D., Van
            den Boomen,D.J.H., Edgar,J.R., Bang,J.H., Predeus,A.,
            Teichmann,S.A., Marioni,J.C., Matesic,L.E., Lee,J.H., Lehner,P.J.,
            Marciniak,S.J., Rawlins,E.L. and Dickens,J.A.
  TITLE     A novel human fetal lung-derived alveolar organoid model reveals
            mechanisms of surfactant protein C maturation relevant to
            interstitial lung disease
  JOURNAL   EMBO J 44 (3), 639-664 (2025)
   PUBMED   39815007
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5187)
  AUTHORS   Takahashi,S., Ochiai,S., Jin,J., Takahashi,N., Toshima,S.,
            Ishigame,H., Kabashima,K., Kubo,M., Nakayama,M., Shiroguchi,K. and
            Okada,T.
  TITLE     Sensory neuronal STAT3 is critical for IL-31 receptor expression
            and inflammatory itch
  JOURNAL   Cell Rep 42 (12), 113433 (2023)
   PUBMED   38029739
  REMARK    GeneRIF: Sensory neuronal STAT3 is critical for IL-31 receptor
            expression and inflammatory itch.
REFERENCE   4  (bases 1 to 5187)
  AUTHORS   Chowdhury,S., Singh,A.K., Srivastava,S., Upadhyay,V., Sethi,A.,
            Siddiqui,S. and Trivedi,A.K.
  TITLE     AIP4 regulates adipocyte differentiation by targeting C/EBPalpha
            for ubiquitin-mediated proteasomal degradation
  JOURNAL   J Cell Biochem 124 (7), 961-973 (2023)
   PUBMED   37204112
  REMARK    GeneRIF: AIP4 regulates adipocyte differentiation by targeting
            C/EBPalpha for ubiquitin-mediated proteasomal degradation.
REFERENCE   5  (bases 1 to 5187)
  AUTHORS   Ye,W., Miao,Q., Xu,G., Jin,K., Li,X., Wu,W., Yu,L. and Yan,M.
  TITLE     CircRNA itchy E3 ubiquitin protein ligase improves mitochondrial
            dysfunction in sepsis-induced acute kidney injury by targeting
            microRNA-214-3p/ATP-binding cassette A1 axis
  JOURNAL   Ren Fail 45 (2), 2261552 (2023)
   PUBMED   37782276
  REMARK    GeneRIF: CircRNA itchy E3 ubiquitin protein ligase improves
            mitochondrial dysfunction in sepsis-induced acute kidney injury by
            targeting microRNA-214-3p/ATP-binding cassette A1 axis.
REFERENCE   6  (bases 1 to 5187)
  AUTHORS   Traweger,A., Fang,D., Liu,Y.C., Stelzhammer,W., Krizbai,I.A.,
            Fresser,F., Bauer,H.C. and Bauer,H.
  TITLE     The tight junction-specific protein occludin is a functional target
            of the E3 ubiquitin-protein ligase itch
  JOURNAL   J Biol Chem 277 (12), 10201-10208 (2002)
   PUBMED   11782481
REFERENCE   7  (bases 1 to 5187)
  AUTHORS   Harvey,K.F., Shearwin-Whyatt,L.M., Fotia,A., Parton,R.G. and
            Kumar,S.
  TITLE     N4WBP5, a potential target for ubiquitination by the Nedd4 family
            of proteins, is a novel Golgi-associated protein
  JOURNAL   J Biol Chem 277 (11), 9307-9317 (2002)
   PUBMED   11748237
REFERENCE   8  (bases 1 to 5187)
  AUTHORS   Fang,S., Ferrone,M., Yang,C., Jensen,J.P., Tiwari,S. and
            Weissman,A.M.
  TITLE     The tumor autocrine motility factor receptor, gp78, is a ubiquitin
            protein ligase implicated in degradation from the endoplasmic
            reticulum
  JOURNAL   Proc Natl Acad Sci U S A 98 (25), 14422-14427 (2001)
   PUBMED   11724934
REFERENCE   9  (bases 1 to 5187)
  AUTHORS   Qiu,L., Joazeiro,C., Fang,N., Wang,H.Y., Elly,C., Altman,Y.,
            Fang,D., Hunter,T. and Liu,Y.C.
  TITLE     Recognition and ubiquitination of Notch by Itch, a hect-type E3
            ubiquitin ligase
  JOURNAL   J Biol Chem 275 (46), 35734-35737 (2000)
   PUBMED   10940313
REFERENCE   10 (bases 1 to 5187)
  AUTHORS   Hustad,C.M., Perry,W.L., Siracusa,L.D., Rasberry,C., Cobb,L.,
            Cattanach,B.M., Kovatch,R., Copeland,N.G. and Jenkins,N.A.
  TITLE     Molecular genetic characterization of six recessive viable alleles
            of the mouse agouti locus
  JOURNAL   Genetics 140 (1), 255-265 (1995)
   PUBMED   7635290
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ122836.1, BC062934.1 and BF453521.1.
            
            On Aug 20, 2011 this sequence version replaced NM_008395.2.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062934.1, AF037454.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                CJ122836.1         1-51
            52-5186             BC062934.1         1-5135
            5187-5187           BF453521.1         385-385
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 76.94 cM"
     gene            1..5187
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /note="itchy, E3 ubiquitin protein ligase"
                     /db_xref="GeneID:16396"
                     /db_xref="MGI:MGI:1202301"
     exon            1..105
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            106..182
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            183..273
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             204..2798
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /EC_number="2.3.2.26"
                     /note="HECT-type E3 ubiquitin transferase Itchy homolog"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Itchy"
                     /protein_id="NP_032421.2"
                     /db_xref="CCDS:CCDS38293.1"
                     /db_xref="GeneID:16396"
                     /db_xref="MGI:MGI:1202301"
                     /translation="MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVE
                     VTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDI
                     YETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSEST
                     TQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRP
                     PPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEGATSGLIIPLTISGGS
                     GPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGR
                     IYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQN
                     KEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFT
                     VDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQH
                     IKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVL
                     NPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPF
                     YKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLK
                     PNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELE
                     VLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRL
                     PVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIE
                     ETEGFGQE"
     exon            274..415
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            416..540
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            541..678
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            679..724
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            725..882
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1078
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1174
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1349
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1419
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1504
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1633
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1706
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1778
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1867
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..2027
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2028..2161
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2302
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2423
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2528
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2625
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2698
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2699..5187
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3529..3534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3549
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
     regulatory      5166..5171
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5187
                     /gene="Itch"
                     /gene_synonym="6720481N21Rik; 8030492O04Rik; A130065M08;
                     AIP4; C230047C07Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcctgcgcac taggtgtgaa gtagctctag tcgctgtagt cggggctcgg gaccggctgc
       61 catcttagcg gagggactga ggagtcgtcg ccgccccgag tcccggtaac atgcatttca
      121 cagtggcctt ctggaaacaa cgccttaacc ctaagaagtg actcagccat tgagaacttc
      181 aggtttgctg tcttattgat ggtatgtctg acagtggacc acagcttgat tccatgggta
      241 gtctgaccat gaaatctcaa cttcagatca ctgtcatctc agccaaactt aaagaaaata
      301 aaaagaattg gtttggacca agtccttatg tagaagtcac agtagatgga cagtcaaaga
      361 agacagaaaa atgcaataat acaaacagtc ccaagtggaa gcagcccctc acagttattg
      421 ttacccctac gagtaaatta tgttttcgtg tgtggagtca ccagaccctg aagtctgatg
      481 ttttattggg aactgctgga ttagatattt atgaaacatt aaagtcaaac aatatgaaac
      541 ttgaagaagt agttatgact ttgcagcttg taggtgacaa agagccaaca gagacgatgg
      601 gagatttgtc agtttgtctt gatgggctgc aagtagaagc tgaggttgtt actaacggtg
      661 aaacgtcatg ctccgagagt actacacaga atgatgatgg ctgcagaacc agagatgata
      721 caagagtgag cacaaatgga tcagaggacc ctgaggttgc agcgtcaggg gaaaacaaga
      781 gggccaatgg gaacaattct ccgtcccttt caaatggtgg ttttaagcct tctagacctc
      841 ctagaccttc gagaccacct ccacccaccc cacgaagacc agcttctgtc aatggctcac
      901 catccacgaa ttctgacagt gatggatcta gtacaggctc cttgccacca acaaatacaa
      961 atgtaaatac aagtacatct gaaggagcaa catctggatt aataattcct cttactatat
     1021 ctggaggctc gggccctagg cctctgaata ctgtaagcca agctccccta ccacctgggt
     1081 gggagcagag agtagaccag catgggcgtg tttactatgt agaccatgtt gaaaagcgaa
     1141 caacatggga tagaccagaa cctctacctc ctggctggga acggcgtgtg gacaatatgg
     1201 gacgtattta ttatgttgat catttcacaa gaaccacaac atggcagagg ccaaccttgg
     1261 aatctgtccg gaactatgaa cagtggcagc tacagcgtag tcagcttcag ggagcaatgc
     1321 agcagtttaa ccagagattc atctatggga atcaagattt gtttgctaca tcacaaaaca
     1381 aagaatttga tccgcttggt ccgttgcccc ctggatggga gaagagaact gatagcaacg
     1441 gcagagtgta ttttgtcaac cacaacactc ggattactca gtgggaagac cccagaagcc
     1501 aaggtcagtt aaatgaaaag cccttaccag aaggctggga aatgagattc acagtggatg
     1561 gaattccata ttttgtggac cacaatagaa gagcaactac ttatatagat ccacgaacgg
     1621 gaaaatcagc cttagacaat gggccccaga tagcctatgt gcgggacttc aaggcaaaag
     1681 ttcagtattt ccggttctgg tgccagcaac tggccatgcc acagcatata aagatcacag
     1741 tgacaagaaa aacattgttt gaggattcct ttcagcagat catgagcttc agcccgcaag
     1801 acctgagaag acgtttgtgg gtgattttcc caggagaaga aggtttagat tatggaggtg
     1861 tagcaagaga atggttcttt cttttgtcac atgaagtgtt gaacccaatg tattgcctgt
     1921 ttgaatatgc agggaaggat aactactgcc tgcagataaa ccccgcttct tacatcaatc
     1981 cagaccacct gaaatacttt cgttttattg gcagatttat tgccatggct ctgtttcatg
     2041 ggaaattcat agataccgga ttttctttac cattctataa acgtatcctg aataaaccag
     2101 ttggactgaa ggatttagaa tctattgatc cagaatttta taattcgctc atctgggtta
     2161 aagaaaacaa cattgaagaa tgtggtttgg aaatgtactt ctcagttgat aaagaaattc
     2221 taggtgaaat taagagtcat gatttgaaac ccaatggtgg caatattctt gtgacagaag
     2281 aaaacaagga ggaatacatc aggatggtag ctgagtggag gttgtctcga ggtgttgaag
     2341 aacagacaca agctttcttt gagggcttta atgaaattct tcctcagcaa tatttgcaat
     2401 attttgatgc gaaggaatta gaggttcttc tgtgtgggat gcaggagatt gatttgaatg
     2461 actggcagag acatgccatc taccgccact acaccagaac aagcaagcag atcatgtggt
     2521 tttggcagtt tgttaaagaa attgataatg agaagaggat gagacttctg cagtttgtta
     2581 ctggaacctg ccgattgcca gtgggaggat ttgctgacct tatggggagt aatggaccac
     2641 agaagttctg catcgaaaaa gttggcaaag aaaattggtt acccagaagc catacttgtt
     2701 ttaaccgcct ggacctccca ccttacaaga gctatgagca actgaaggaa aagctgttat
     2761 ttgccattga agaaactgaa ggatttggac aagagtaatt gtttagacct taagccacca
     2821 gaggccaggg acttgtctgc agtgtctgtc cccttccttc tctgcctgtt gcacatctta
     2881 ttgtaaactt ggactgtgac tagagagtta cctgagagta agtaaattaa tgttatcgtt
     2941 gagatcttcc agtcattctg gatttctact caacgtttcc agaaagcaga tctacaaact
     3001 gtagtcagaa acttaacatg agatttaaca cagcgaggaa atttccctcg tcttattcca
     3061 ctagattgtt cccttaacaa cagtttgctg tatgtccgaa gtctcacttg ggcataggtc
     3121 tcttagtggg tattgtaaag gattctgcag ggacatctcg gaaacgcaat acacaaggtc
     3181 ttcctgtaaa gcctcgctca gaggtgcaag cttaactcag tgtctgggca catgagcagc
     3241 tgctgcttgt gctctccgct cgtcccagcc tggcctgact gttgtttttc attcctggag
     3301 catgaatcag tgcaccattc tgatgctatt cctggaagtg atgcaggact tgcatttttc
     3361 tacaaagtag ttttgtctat ttgaattcag ggaaaacttg gtccccgcca acatatgacc
     3421 atacctctct tcattcagtt tgtaattgtt ctattgcctt gcaaatacta aactttacaa
     3481 acagtatcag cactgtggtt ccttcacgtt tttagaaatt aacctagaat taaaataagt
     3541 tcagatgaat tcatctaaga tcattcttga tttttttaaa tcctacacat aaattgaagc
     3601 tttatggtgt tgaagtaatc caacacctta gttacatttc atttttgcta aactattcaa
     3661 gttgcaacca tttcctaaca caaatggagg tcagtggtgc agactctgca gtcacaacag
     3721 cgtccagcag ccacacagct gcgctgtctt cacactccac tcctgacaca ggagcagccg
     3781 tgtctaccct cttacatggt cctgatctac aatatttttc tctcccctaa aaattgtaga
     3841 tttccctttt ctttccatga ctcctttatc ttaggctgct cctttagtaa ctgatgaatg
     3901 cactttgacc ttctctgtag gtcatttacc ttggcttagc attagactgt gaggatggtt
     3961 gctgcccaga ctttatgggc agcaagatga aacctcagcc aggtgagtcc ctattgtagc
     4021 caaatcccaa aattagcctt atttctctaa tggggacctt gaaatgtgtt ggtggcatag
     4081 cataaagcca agaacaccaa gtgtgacaaa cccctgtccc ataggaaaga aagtgtttct
     4141 gtgtccctag ctgctcatcc tgttctcatc ctgggacagt ttgaactcgt gcagctgtgc
     4201 tccttaacac tgccatcctc agcagagacg tgacagacgg gttgcctcat gaagaagtga
     4261 tcagtgaagg gagagctcgg ctcctcccca gccctctttt tacccttata ccacaagaaa
     4321 tacatgtcat ctctagaagc cctagttctt tccaaagtag gaaatctctg cctttttgtt
     4381 ggtagggtta aaaaaaaatg tatatgctaa ttgctttgtc ttacagagcg aatgtctgcc
     4441 aactatccat tcactacata ggcctgaact ttggtaataa atggctataa atattaagcc
     4501 ttctacaaag acttcctatt gaatccaatt cttgtactaa aacatagtta tctaccatat
     4561 tcactagaga tttatgagat taaattaaga gataatgtaa gaaaatagac tttgttgatt
     4621 ctgcataatg cttcatgatt catttaggga cctagaaata ttatgtgaaa atgtataaat
     4681 atcatccaaa aggctttctg ccctatattt ttaaaataca gaagttatat ttgaagtagt
     4741 cctggcccta gttctatagg gcttggctat ttaatatttt tatggaagaa acgtttagtt
     4801 ctggaaaagg taaatgcttg tatatatttt tgcagccctg gagtctccca actccatttc
     4861 ttcctttaat ttaattgatc acatgtatat gtcttccctg ctgtgttaga aaaacggggg
     4921 ctggatatcc caggagtcag agcgtatata agacaatgca cctaaacagc aggcacattg
     4981 accagatgct ctcaaatttt ggtccaaaat gagtatttgg gaatagattt attgtgaatg
     5041 tttaattagc gattttcctt aaatctggat ggactaagtt gcttttaaga cctttttctc
     5101 tgtaagcatc gtaaatgcta atcaacctgt taagtttttt tttttcttac tgcatgttac
     5161 attgaaataa aaacagagta aaatgag
//
