LOCUS       NM_028018               2352 bp    mRNA    linear   ROD 28-AUG-2024
DEFINITION  Mus musculus increased sodium tolerance 1 homolog (yeast) (Ist1),
            transcript variant 1, mRNA.
ACCESSION   NM_028018
VERSION     NM_028018.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Tang,Q., Fang,Z., Liao,H., Zhang,Y., Li,C., Zhou,C., Liu,F. and
            Shen,J.
  TITLE     Reduced circ_lrrc49 in trigeminal ganglion contributes to
            neuropathic pain in mice by downregulating Ist1 and impairing
            autophagy
  JOURNAL   J Neurochem 168 (7), 1265-1280 (2024)
   PUBMED   38348636
  REMARK    GeneRIF: Reduced circ_lrrc49 in trigeminal ganglion contributes to
            neuropathic pain in mice by downregulating Ist1 and impairing
            autophagy.
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2352)
  AUTHORS   Hermosilla Aguayo,V., Martin,P., Tian,N., Zheng,J., Aho,R., Losa,M.
            and Selleri,L.
  TITLE     ESCRT-dependent control of craniofacial morphogenesis with
            concomitant perturbation of NOTCH signaling
  JOURNAL   Dev Biol 503, 25-42 (2023)
   PUBMED   37573008
REFERENCE   4  (bases 1 to 2352)
  AUTHORS   Feng,Q., Luo,Y., Zhang,X.N., Yang,X.F., Hong,X.Y., Sun,D.S.,
            Li,X.C., Hu,Y., Li,X.G., Zhang,J.F., Li,X., Yang,Y., Wang,Q.,
            Liu,G.P. and Wang,J.Z.
  TITLE     MAPT/Tau accumulation represses autophagy flux by disrupting
            IST1-regulated ESCRT-III complex formation: a vicious cycle in
            Alzheimer neurodegeneration
  JOURNAL   Autophagy 16 (4), 641-658 (2020)
   PUBMED   31223056
REFERENCE   5  (bases 1 to 2352)
  AUTHORS   Nakahara,M., Tateyama,H., Araki,M., Nakagata,N., Yamamura,K. and
            Araki,K.
  TITLE     Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from
            MSM/Ms mice
  JOURNAL   Mamm Genome 24 (5-6), 228-239 (2013)
   PUBMED   23604909
REFERENCE   6  (bases 1 to 2352)
  AUTHORS   Hanson,P.I., Roth,R., Lin,Y. and Heuser,J.E.
  TITLE     Plasma membrane deformation by circular arrays of ESCRT-III protein
            filaments
  JOURNAL   J Cell Biol 180 (2), 389-402 (2008)
   PUBMED   18209100
REFERENCE   7  (bases 1 to 2352)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   8  (bases 1 to 2352)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   9  (bases 1 to 2352)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2352)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY160969.1, AK010262.1 and BE947178.1.
            
            On Jul 18, 2009 this sequence version replaced NM_028018.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017605.1, AK010262.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BY160969.1         2-31
            31-2334             AK010262.1         1-2304
            2335-2352           BE947178.1         7-24                c
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 57.18 cM"
     gene            1..2352
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /note="increased sodium tolerance 1 homolog (yeast)"
                     /db_xref="GeneID:71955"
                     /db_xref="MGI:MGI:1919205"
     exon            1..111
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            112..214
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..1215
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /note="isoform 1 is encoded by transcript variant 1; IST1
                     homolog; charged multivesicular body protein 8"
                     /codon_start=1
                     /product="IST1 homolog isoform 1"
                     /protein_id="NP_082294.1"
                     /db_xref="CCDS:CCDS22655.1"
                     /db_xref="GeneID:71955"
                     /db_xref="MGI:MGI:1919205"
                     /translation="MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYL
                     AAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVST
                     LIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKI
                     LVERYLIEIAKNYNVPYEPDSVVMAEAPVGVETDLIDVGFTDDVKKGGPGRGGGGGFT
                     APVGGPDGIVPMPMPMPMPSPNAPFAYPLPKGPSDFSGLPVGTYQAFPNIHPPQIPAT
                     PPSYESVDDINGDKTVSSAQIVGPKPEAPAKPPSRPVDNYNTFVLPELPSVPDTLPTA
                     SAGASTSASEDIDFDDLSRRFEELKKKT"
     exon            215..395
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            396..483
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            484..567
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            568..678
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            679..879
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            880..972
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1021
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..2342
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2314..2319
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /note="hexamer: AATAAA"
     polyA_site      2342
                     /gene="Ist1"
                     /gene_synonym="2400003C14Rik; CHMP8; mKIAA0174"
                     /note="major polyA site"
ORIGIN      
        1 ggattcctcc gctcgagtcc gcggtgggga ggtcgaggga gtcgccattt tggatgccga
       61 gcggctcctc ggaagtcggc ttcttccgtt ttcacgtccg gatccctcca ggaggagcag
      121 gacaacatgc tgggctctgg gttcaaagct gagcgcttac gagtcaattt gagattagtt
      181 ataaatcgtc tcaaactgtt ggagaaaaaa aaaacggaac tggctcagaa ggcaaggaag
      241 gagattgctg actacctggc cgccggaaag gatgagcgag cacgcatccg tgtggagcac
      301 ataatccggg aggactacct cgtggaggcc atggagatcc tggagctgta ttgtgacctg
      361 ctgctggcgc gctttggcct tatccagtct atgaaggagc tagattctgg tctagctgaa
      421 tccgtgtcta cactgatctg ggctgcgcct cggcttcagt cagaagtggc tgagttgaaa
      481 atagttgccg atcagctctg tgccaaatac agcaaggaat atggcaagtt gtgcaggacc
      541 aaccagatcg ggactgtgaa tgataggcta atgcacaaac tgagcgtaga agccccaccc
      601 aaaatcctag tggagagata cctgattgaa attgccaaga attacaatgt accctacgaa
      661 cctgactctg tggtcatggc agaagctcct gttggggtgg agacagatct tattgatgtt
      721 ggatttacgg acgatgtgaa gaaaggtggt cctggaagag gaggaggggg cgggttcaca
      781 gcccccgttg ggggacctga tgggattgtg cccatgccca tgcctatgcc catgccatct
      841 ccaaatgctc ccttcgcata cccactgcca aagggaccat cggatttcag tggattgcca
      901 gtggggactt accaggcctt tcctaacatt cacccacctc agataccagc aactccccca
      961 tcctatgaat ctgttgatga cattaatggt gataaaactg tttcttctgc acagattgtt
     1021 ggacccaagc cagaagctcc tgcaaagccc ccttccagac ctgtggataa ttacaacacc
     1081 tttgtactgc cagagttgcc atcggtgcca gacacacttc caactgcatc tgctggagcc
     1141 agcacctcag catcagaaga catagacttt gatgatcttt cccggagatt tgaggaactg
     1201 aaaaagaaaa cataggtctc ttaaaccagg cagcatccac attctggaag ttgatagtga
     1261 acagttctcc gtgtaacagt gactctccat gaaattccgt ttcctctgtt aaccgtcact
     1321 gagcactctt cctcggggct ctctgctgct ctaccaattg tgttgctttc cacttcttca
     1381 gtgattcttc agtgatcctg agagactaac agctggagac tctagctgga gcagttggct
     1441 cctattggct gtgtttgcct gtctgtcttc ctgtcacgtg gatccttggt tctttcccgg
     1501 ccattccatt cccagggagt gagaagtatt gtgggtagag ttgccaaaga tggctggacc
     1561 atggaagaga gtaccctatg atgggtttca accttgactc gtaaatactt ccctctgcat
     1621 gggggtgatc gtagagagaa tcaaggctac catgtgtttt ctcatgagtc agaagatggc
     1681 tcaggaagcc actgtcacgg ttcctggagg ctgggccctc tcattgacat gtacactact
     1741 cctcgctgca gggcactgtt ccacctggat ccagttgcaa agtttggaaa agttctctta
     1801 gttttactgg attctcaggg agccctctgt ggccttttcc tttattttga gtgctgtttc
     1861 ctttttagga aagaacagca ctgtggaaat ttgcttttcc ccatagcatg ttaggaagga
     1921 agtacaggtc gttacctaag ctagcgtggg cttccagttg ccttaataga agtactcaag
     1981 tctcttgggt agagatctgg aagcctgcag gagaggaggg gtacttgttt tcattggaaa
     2041 acttttatga ttaagggact ctttacaaac tggcatctta agttttagtg ccatgtcgct
     2101 gtagtctgct gctttcctgg gtggatgaac ctctttatta tggtaacttc tgtgctgctt
     2161 tgcccctagc caaggctctc cttccccaag aggttagctt tggcattgga agatgagatt
     2221 tttgcttacc tgtagtattt ctgtacatac ctgtttccag caacttcagt gtcaatgttt
     2281 ttgatttatt tgtgtcttat agctctagct gctaataaag atcccatgtc ctgctttctc
     2341 taaaaaaaaa aa
//
