LOCUS       NM_001290302            2526 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus isthmin 2 (Ism2), mRNA.
ACCESSION   NM_001290302 XM_006516432 XM_006542998
VERSION     NM_001290302.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Du,J., Takeuchi,H., Leonhard-Melief,C., Shroyer,K.R., Dlugosz,M.,
            Haltiwanger,R.S. and Holdener,B.C.
  TITLE     O-fucosylation of thrombospondin type 1 repeats restricts
            epithelial to mesenchymal transition (EMT) and maintains epiblast
            pluripotency during mouse gastrulation
  JOURNAL   Dev Biol 346 (1), 25-38 (2010)
   PUBMED   20637190
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT030249.7, AC151967.6, BU054611.1 and BQ032954.1.
            
            On or before Mar 12, 2014 this sequence version replaced
            XM_006516432.1, XM_006542998.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The exon combination of this
            RefSeq is inferred based on partial mouse transcript alignments,
            RNAseq data, and full-length orthologous transcript alignments.
            
            CCDS Note: The exon combination of this CCDS representation is
            inferred due to a lack of full-length transcript support for this
            gene. It is supported by partial mouse transcript alignments,
            RNA-seq data, and full-length orthologous transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.933903.1,
                                           SRR14995048.478730.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-132               CT030249.7         2942-3073           c
            133-242             AC151967.6         180557-180666       c
            243-772             BU054611.1         48-577
            773-877             AC151967.6         176175-176279       c
            878-961             AC151967.6         175829-175912       c
            962-2015            AC151967.6         172678-173731       c
            2016-2522           BQ032954.1         16-522              c
            2523-2526           AC151967.6         172167-172170       c
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 41.49 cM"
     gene            1..2526
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /note="isthmin 2"
                     /db_xref="GeneID:217738"
                     /db_xref="MGI:MGI:2685110"
     CDS             1..1482
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /note="thrombospondin, type I, domain containing 3;
                     isthmin 2 homolog"
                     /codon_start=1
                     /product="isthmin-2 precursor"
                     /protein_id="NP_001277231.1"
                     /db_xref="CCDS:CCDS70410.1"
                     /db_xref="GeneID:217738"
                     /db_xref="MGI:MGI:2685110"
                     /translation="MRPPPSRARFPRALMLLAALLATGRGLPLRKRGPGPHGHSRMAE
                     ASPAPDLSSPKEKNTTPGFPGDHLQTEPCQPGCQTIPEPAALTLGTATSPRTLEHTPL
                     LLELQKLPGLANTDLSAPNPNIQVTIEVVQDPQTEVEMDLPSEPSNLWPLHAPSWLPT
                     KEFFWPLFWGYQEGEEGATSLRDRAPGEAEVEGGKDYAVEYGEGEDQRGTEEGEAEEP
                     WSSGAIDNWDYGWLGPQEQDFQQPDSYDCEPQEEWSTWSPCNGSCGSGYQQRTRPCGY
                     ACTATESRVCDLAPCPGTAEENTLGFPSEGWQPLAHNATDMLDPDVDSCEKWLNCKSD
                     FLANYLSQVLKDLPSCPCAYPLEAVYSAAVSLQDEHQGRSFQWRDASSPQERLDIYQP
                     TARYCLRSMLSSESSTLAAQHCCYDEGSRLLTRGKGAGAPDLVSTDFSPELHFKVDRL
                     PWILCKGDWSRYHTVRPPNNGRACTDNPPEEEYLAQLQEAKEY"
     exon            1..132
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /inference="alignment:Splign:2.1.0"
     exon            133..375
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /inference="alignment:Splign:2.1.0"
     exon            376..736
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /inference="alignment:Splign:2.1.0"
     exon            737..877
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /inference="alignment:Splign:2.1.0"
     exon            878..961
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /inference="alignment:Splign:2.1.0"
     exon            962..2526
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2501..2506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /note="hexamer: AATAAA"
     polyA_site      2520
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /note="major polyA site"
ORIGIN      
        1 atgcggccac cgcccagccg agcacggttc ccgcgcgcct tgatgctgct ggccgccctg
       61 ctggccactg gccgagggct cccactgagg aagcgaggtc ccgggcccca cggccactcg
      121 aggatggcgg aggcctcccc tgccccagat ctcagctccc caaaggagaa gaacacaaca
      181 ccaggattcc ctggagacca tctgcagaca gaaccatgtc agcccgggtg ccagaccatc
      241 cctgagccag cagctctgac tctgggtaca gccacatctc ccaggaccct ggaacacacc
      301 cctctgctgc tggagctgca gaagctacca gggttggcca acacagactt gagcgcacca
      361 aaccccaaca tccaggtgac catcgaggtg gtgcaggacc ctcagactga ggtagagatg
      421 gacctgcctt ctgagcccag caatctctgg cccctgcatg cccctagctg gttgcccacc
      481 aaggaattct tttggcccct cttctggggc taccaggaag gtgaagaagg agccaccagc
      541 ctcagagaca gggctccagg ggaagcagag gtggaaggag gaaaggacta tgctgtggag
      601 tatggcgagg gtgaggacca aaggggaact gaagaaggcg aggctgagga gccttggtcc
      661 agcggggcca tagacaactg ggactatggc tggttgggtc ctcaggaaca agacttccaa
      721 caaccagaca gctatgactg tgaaccccag gaggagtgga gcacctggtc tccctgcaac
      781 gggagctgcg gcagcggata ccagcagaga acgcgaccct gcggctatgc ctgcactgcc
      841 acagagtccc gagtctgcga ccttgccccc tgtcctggca ccgcagagga gaacacgttg
      901 ggctttccca gtgaggggtg gcagcccctt gcccacaatg caacggatat gcttgatcca
      961 gatgtggaca gctgtgagaa gtggctgaac tgcaagagtg acttcctagc taactacctg
     1021 agccaagtgc tcaaggacct gcccagttgc ccgtgtgcat accctctgga ggcagtgtac
     1081 agcgccgccg tgagcctgca ggacgaacat cagggtcgca gcttccagtg gcgggatgcg
     1141 agtagcccac aagagcgctt ggacatctac cagcctactg cgcgctactg cctgcgttcc
     1201 atgctgtcta gtgagagcag caccttggct gcccagcact gctgctatga tgaaggcagc
     1261 cggttgctga cccgtggcaa aggggcaggg gccccggact tagtcagcac tgacttttca
     1321 cctgagcttc acttcaaggt agataggctg ccctggatcc tgtgtaaggg ggactggagc
     1381 cgctaccaca ctgtgcgccc ccccaacaat ggccgggctt gcacagacaa cccaccagag
     1441 gaagagtacc tggctcaatt gcaggaggcc aaagagtact aaagaggaat tagagcatgc
     1501 tccttctgcc ctttaccctg cccctgctca gactgggcaa ctattagggc tcatctgaga
     1561 ggccgtcagg catgcatatc tccctcttat gaccctgagt caggagaact caagcgtctc
     1621 agggtctggt gagaaggttg gagggtgtgt caggcacaag gcctctggga tgtgttggga
     1681 aggggaggag ctatcccagt ttggccatgg gtctttccct ctctctagga atggggccct
     1741 tctgcctttc cctctgttcg ggattgagcc aaggtggtcc agacacatcc cagttcccag
     1801 gatctgttgt gaatccctta gacccccacc ccacccaccc atctacccta gggccagttg
     1861 tggaagaaca cagggagggg tggggcaaga taggacagtc ctttcatctg accctaaagc
     1921 tgctttttgc tgggctggca gaaccagggt gaccacccct ccttgtggca cacagcttct
     1981 accctgtccc atgtggtgag gcggtcagcg gagctgaggg gagattgctt ccagtcaggg
     2041 aaccaccagc tgtttcccgt ccccagatga tagacatgcg caggctacca ccgccatggc
     2101 agccaggctt gggttgaact cagtactaag ggtgggttca ggagggatca tgcacctgtc
     2161 gcaagtgtga cagggcagag ggtcccgata ttggatgtgg gccatttatg gtgttggtac
     2221 ccacaccagt tgcccactta acgccccccc gcccccccac cccgtgtggc acccctgaaa
     2281 gcactggact tggacttggg agatgggtgc tttggctccc agcacactgt gccttttccc
     2341 ggacacctct catccccaaa ccatactttc ttggtttctc tggagctgaa atattgtaga
     2401 ctttctgttt ctattcacaa atctccgata ggggcttggt agggaacttg agttccagcc
     2461 ccatccacag ctgtccagaa gctgtcctgg gatcctcagc aataaagcac tttattttta
     2521 cataca
//
