LOCUS       NP_001277231             493 aa            linear   ROD 30-APR-2024
DEFINITION  isthmin-2 precursor [Mus musculus].
ACCESSION   NP_001277231 XP_006516495 XP_006543061
VERSION     NP_001277231.1
DBSOURCE    REFSEQ: accession NM_001290302.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 493)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 493)
  AUTHORS   Du,J., Takeuchi,H., Leonhard-Melief,C., Shroyer,K.R., Dlugosz,M.,
            Haltiwanger,R.S. and Holdener,B.C.
  TITLE     O-fucosylation of thrombospondin type 1 repeats restricts
            epithelial to mesenchymal transition (EMT) and maintains epiblast
            pluripotency during mouse gastrulation
  JOURNAL   Dev Biol 346 (1), 25-38 (2010)
   PUBMED   20637190
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT030249.7, AC151967.6, BU054611.1 and BQ032954.1.
            
            On or before Mar 12, 2014 this sequence version replaced
            XP_006516495.1, XP_006543061.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The exon combination of this
            RefSeq is inferred based on partial mouse transcript alignments,
            RNAseq data, and full-length orthologous transcript alignments.
            
            CCDS Note: The exon combination of this CCDS representation is
            inferred due to a lack of full-length transcript support for this
            gene. It is supported by partial mouse transcript alignments,
            RNA-seq data, and full-length orthologous transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.933903.1,
                                           SRR14995048.478730.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 41.49 cM"
     Protein         1..493
                     /product="isthmin-2 precursor"
                     /note="thrombospondin, type I, domain containing 3;
                     isthmin 2 homolog"
                     /calculated_mol_wt=51728
     sig_peptide     1..26
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2820
     Region          252..292
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          321..481
                     /region_name="AMOP"
                     /note="AMOP domain; cl02758"
                     /db_xref="CDD:470667"
     CDS             1..493
                     /gene="Ism2"
                     /gene_synonym="Gm264; Thsd3"
                     /coded_by="NM_001290302.1:1..1482"
                     /db_xref="CCDS:CCDS70410.1"
                     /db_xref="GeneID:217738"
                     /db_xref="MGI:MGI:2685110"
ORIGIN      
        1 mrpppsrarf pralmllaal latgrglplr krgpgphghs rmaeaspapd lsspkekntt
       61 pgfpgdhlqt epcqpgcqti pepaaltlgt atsprtleht plllelqklp glantdlsap
      121 npniqvtiev vqdpqtevem dlpsepsnlw plhapswlpt keffwplfwg yqegeegats
      181 lrdrapgeae veggkdyave ygegedqrgt eegeaeepws sgaidnwdyg wlgpqeqdfq
      241 qpdsydcepq eewstwspcn gscgsgyqqr trpcgyacta tesrvcdlap cpgtaeentl
      301 gfpsegwqpl ahnatdmldp dvdscekwln cksdflanyl sqvlkdlpsc pcaypleavy
      361 saavslqdeh qgrsfqwrda sspqerldiy qptaryclrs mlssesstla aqhccydegs
      421 rlltrgkgag apdlvstdfs pelhfkvdrl pwilckgdws ryhtvrppnn gractdnppe
      481 eeylaqlqea key
//
