LOCUS       NM_015783                756 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus ISG15 ubiquitin-like modifier (Isg15), mRNA.
ACCESSION   NM_015783 XM_001471685 XM_001471686
VERSION     NM_015783.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Wei,W., Gao,X., Qian,J., Li,L., Zhao,C., Xu,L., Zhu,Y., Liu,Z.,
            Liu,N., Wang,X., Jin,Z., Liu,B., Xu,L., Dong,J., Zhang,S., Wang,J.,
            Zhang,Y., Yu,Y., Yan,Z., Yang,Y., Lu,J., Fang,Y., Yuan,N. and
            Wang,J.
  TITLE     Beclin 1 prevents ISG15-mediated cytokine storms to secure fetal
            hematopoiesis and survival
  JOURNAL   J Clin Invest 135 (3), e177375 (2025)
   PUBMED   39589832
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  AUTHORS   Zerad,L., Gacem,N., Gayda,F., Day,L., Sinigaglia,K., Richard,L.,
            Parisot,M., Cagnard,N., Mathis,S., Bole-Feysot,C., O'Connell,M.A.,
            Pingault,V., Dambroise,E., Keegan,L.P., Vallat,J.M. and
            Bondurand,N.
  TITLE     Overexpression of Egr1 Transcription Regulator Contributes to
            Schwann Cell Differentiation Defects in Neural Crest-Specific Adar1
            Knockout Mice
  JOURNAL   Cells 13 (23), 1952 (2024)
   PUBMED   39682701
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 756)
  AUTHORS   Cui,N., Liu,C., Tang,X., Song,L., Xiao,Z., Wang,C., Wu,Y., Zhou,Y.,
            Peng,C., Liu,Y., Zheng,L., Liu,X., Huang,K. and Chen,H.
  TITLE     ISG15 accelerates acute kidney injury and the subsequent AKI-to-CKD
            transition by promoting TGFbetaR1 ISGylation
  JOURNAL   Theranostics 14 (11), 4536-4553 (2024)
   PUBMED   39113797
  REMARK    GeneRIF: ISG15 accelerates acute kidney injury and the subsequent
            AKI-to-CKD transition by promoting TGFbetaR1 ISGylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 756)
  AUTHORS   Homma,H., Yoshioka,Y., Fujita,K., Shirai,S., Hama,Y., Komano,H.,
            Saito,Y., Yabe,I., Okano,H., Sasaki,H., Tanaka,H. and Okazawa,H.
  TITLE     Dynamic molecular network analysis of iPSC-Purkinje cells
            differentiation delineates roles of ISG15 in SCA1 at the earliest
            stage
  JOURNAL   Commun Biol 7 (1), 413 (2024)
   PUBMED   38594382
  REMARK    GeneRIF: Dynamic molecular network analysis of iPSC-Purkinje cells
            differentiation delineates roles of ISG15 in SCA1 at the earliest
            stage.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 756)
  AUTHORS   Chu,L., Qian,L., Chen,Y., Duan,S., Ding,M., Sun,W., Meng,W.,
            Zhu,J., Wang,Q., Hao,H., Wang,C. and Cui,S.
  TITLE     HERC5-catalyzed ISGylation potentiates cGAS-mediated innate
            immunity
  JOURNAL   Cell Rep 43 (3), 113870 (2024)
   PUBMED   38421872
REFERENCE   6  (bases 1 to 756)
  AUTHORS   Liu,M., Reimschuessel,R. and Hassel,B.A.
  TITLE     Molecular cloning of the fish interferon stimulated gene, 15 kDa
            (ISG15) orthologue: a ubiquitin-like gene induced by nephrotoxic
            damage
  JOURNAL   Gene 298 (2), 129-139 (2002)
   PUBMED   12426101
REFERENCE   7  (bases 1 to 756)
  AUTHORS   Ritchie,K.J., Malakhov,M.P., Hetherington,C.J., Zhou,L.,
            Little,M.T., Malakhova,O.A., Sipe,J.C., Orkin,S.H. and Zhang,D.E.
  TITLE     Dysregulation of protein modification by ISG15 results in brain
            cell injury
  JOURNAL   Genes Dev 16 (17), 2207-2212 (2002)
   PUBMED   12208842
  REMARK    GeneRIF: UBP43 plays a critical role in maintaining the homeostatic
            balance of ISG15-conjugated protein, and that regulation of
            cellular levels of ISG15 protein modification is essential for
            brain cell function.
REFERENCE   8  (bases 1 to 756)
  AUTHORS   Malakhova,O., Malakhov,M., Hetherington,C. and Zhang,D.E.
  TITLE     Lipopolysaccharide activates the expression of ISG15-specific
            protease UBP43 via interferon regulatory factor 3
  JOURNAL   J Biol Chem 277 (17), 14703-14711 (2002)
   PUBMED   11854279
  REMARK    GeneRIF: lipopolysaccharide activation of expression via interferon
            regulatory factor 3
REFERENCE   9  (bases 1 to 756)
  AUTHORS   Malakhov,M.P., Malakhova,O.A., Kim,K.I., Ritchie,K.J. and
            Zhang,D.E.
  TITLE     UBP43 (USP18) specifically removes ISG15 from conjugated proteins
  JOURNAL   J Biol Chem 277 (12), 9976-9981 (2002)
   PUBMED   11788588
  REMARK    GeneRIF: UBP43 (USP18) specifically removes ISG15 from conjugated
            proteins
REFERENCE   10 (bases 1 to 756)
  AUTHORS   Hamerman,J.A., Hayashi,F., Schroeder,L.A., Gygi,S.P., Haas,A.L.,
            Hampson,L., Coughlin,P., Aebersold,R. and Aderem,A.
  TITLE     Serpin 2a is induced in activated macrophages and conjugates to a
            ubiquitin homolog
  JOURNAL   J Immunol 168 (5), 2415-2423 (2002)
   PUBMED   11859133
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT998563.5.
            
            On or before Apr 18, 2009 this sequence version replaced
            XM_001471685.1, XM_001471686.1, NM_015783.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AI117472.1, BC031424.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               CT998563.5         25261-25373         c
            114-756             CT998563.5         23979-24621         c
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 88.61 cM"
     gene            1..756
                     /gene="Isg15"
                     /gene_synonym="100038882; G1p2; IGI15; IP17; Irfp; UCRP"
                     /note="ISG15 ubiquitin-like modifier"
                     /db_xref="GeneID:100038882"
                     /db_xref="MGI:MGI:1855694"
     exon            1..113
                     /gene="Isg15"
                     /gene_synonym="100038882; G1p2; IGI15; IP17; Irfp; UCRP"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..596
                     /gene="Isg15"
                     /gene_synonym="100038882; G1p2; IGI15; IP17; Irfp; UCRP"
                     /note="interferon, alpha-inducible protein;
                     interferon-induced 15-KDa protein; ubiquitin
                     cross-reactive protein; interferon-stimulated protein 15;
                     interferon-induced 15 kDa protein; interferon-induced 17
                     kDa protein; interferon-stimulated protein (15 kDa)"
                     /codon_start=1
                     /product="ubiquitin-like protein ISG15"
                     /protein_id="NP_056598.2"
                     /db_xref="CCDS:CCDS51405.1"
                     /db_xref="GeneID:100038882"
                     /db_xref="MGI:MGI:1855694"
                     /translation="MAWDLKVKMLGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQR
                     LAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTV
                     DTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGLKPQCTVIKHLRLRGGGGDQC
                     A"
     exon            114..756
                     /gene="Isg15"
                     /gene_synonym="100038882; G1p2; IGI15; IP17; Irfp; UCRP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      734..739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Isg15"
                     /gene_synonym="100038882; G1p2; IGI15; IP17; Irfp; UCRP"
                     /note="hexamer: AATAAA"
     polyA_site      754
                     /gene="Isg15"
                     /gene_synonym="100038882; G1p2; IGI15; IP17; Irfp; UCRP"
                     /note="major polyA site"
ORIGIN      
        1 agtcgaccca gtctctgact ctgagagtcg atccagtctc tgactgtgag agcaagcagc
       61 cagaagcaga ctccttaatt ccaggggacc tagagctaga gcctgcagca atggcctggg
      121 acctaaaggt gaagatgctg gggggtaacg atttcctggt gtccgtgact aactccatga
      181 cggtgtcaga actgaagaag cagattgccc agaagattgg tgtgccggct ttccagcagc
      241 gcctggccca ccaaactgca gtgctccagg acggtcttac cctttccagt ctgggtctgg
      301 gtcccagcag cacagtgatg ctagtggtac agaactgcag cgagcctctg agcatcctgg
      361 tgaggaacga aaggggccac agcaacatct atgaggtctt tctgacgcag actgtagaca
      421 cgcttaagaa gaaggtgtcc cagcgggaac aagtccacga agaccagttc tggctgagct
      481 tcgagggaag gcccatggag gacaaggagc tgctggggga gtatggccta aagccccagt
      541 gcacagtgat caagcatttg cgcctgaggg gtgggggagg ggaccagtgt gcctaaggcc
      601 cccatccaga gccagtgttg gttataaaag atgatggggg ggggggtatg gaggactttt
      661 gggatagcat tggaaatgta aacgaggaaa atacctaatt agaaaaaata tcaaaaaaaa
      721 aaaaagatga tgcaataaaa agctctgcct cttgtt
//
