LOCUS       NM_025526                904 bp    mRNA    linear   ROD 06-FEB-2025
DEFINITION  Mus musculus iron-sulfur cluster assembly enzyme (Iscu), transcript
            variant 1, mRNA.
ACCESSION   NM_025526
VERSION     NM_025526.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Roy,A.R., Ahmed,A., DiStefano,P.V., Chi,L., Khyzha,N., Galjart,N.,
            Wilson,M.D., Fish,J.E. and Delgado-Olguin,P.
  TITLE     The transcriptional regulator CCCTC-binding factor limits oxidative
            stress in endothelial cells
  JOURNAL   J Biol Chem 293 (22), 8449-8461 (2018)
   PUBMED   29610276
REFERENCE   2  (bases 1 to 904)
  AUTHORS   Boniecki,M.T., Freibert,S.A., Muhlenhoff,U., Lill,R. and Cygler,M.
  TITLE     Structure and functional dynamics of the mitochondrial Fe/S cluster
            synthesis complex
  JOURNAL   Nat Commun 8 (1), 1287 (2017)
   PUBMED   29097656
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 904)
  AUTHORS   Gakh,O., Ranatunga,W., Smith,D.Y. 4th, Ahlgren,E.C.,
            Al-Karadaghi,S., Thompson,J.R. and Isaya,G.
  TITLE     Architecture of the Human Mitochondrial Iron-Sulfur Cluster
            Assembly Machinery
  JOURNAL   J Biol Chem 291 (40), 21296-21321 (2016)
   PUBMED   27519411
REFERENCE   4  (bases 1 to 904)
  AUTHORS   Funauchi,Y., Tanikawa,C., Yi Lo,P.H., Mori,J., Daigo,Y., Takano,A.,
            Miyagi,Y., Okawa,A., Nakamura,Y. and Matsuda,K.
  TITLE     Regulation of iron homeostasis by the p53-ISCU pathway
  JOURNAL   Sci Rep 5, 16497 (2015)
   PUBMED   26560363
  REMARK    GeneRIF: ISCU expression was decreased in the majority of human
            liver cancer tissues, and its reduced expression was significantly
            associated with p53 mutation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 904)
  AUTHORS   Parent,A., Elduque,X., Cornu,D., Belot,L., Le Caer,J.P.,
            Grandas,A., Toledano,M.B. and D'Autreaux,B.
  TITLE     Mammalian frataxin directly enhances sulfur transfer of NFS1
            persulfide to both ISCU and free thiols
  JOURNAL   Nat Commun 6, 5686 (2015)
   PUBMED   25597503
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 904)
  AUTHORS   Nordin,A., Larsson,E., Thornell,L.E. and Holmberg,M.
  TITLE     Tissue-specific splicing of ISCU results in a skeletal muscle
            phenotype in myopathy with lactic acidosis, while complete loss of
            ISCU results in early embryonic death in mice
  JOURNAL   Hum Genet 129 (4), 371-378 (2011)
   PUBMED   21165651
  REMARK    GeneRIF: Data show that complete loss of ISCU results in early
            embryonic death and confirm a fundamental role for ISCU in mammals.
REFERENCE   7  (bases 1 to 904)
  AUTHORS   Chan,S.Y., Zhang,Y.Y., Hemann,C., Mahoney,C.E., Zweier,J.L. and
            Loscalzo,J.
  TITLE     MicroRNA-210 controls mitochondrial metabolism during hypoxia by
            repressing the iron-sulfur cluster assembly proteins ISCU1/2
  JOURNAL   Cell Metab 10 (4), 273-284 (2009)
   PUBMED   19808020
  REMARK    GeneRIF: Results identify the iron-sulfur cluster assembly proteins
            (ISCU1/2) as direct targets for repression by the hypoxia-induced
            microRNA-210.
REFERENCE   8  (bases 1 to 904)
  AUTHORS   Martelli,A., Wattenhofer-Donze,M., Schmucker,S., Bouvet,S.,
            Reutenauer,L. and Puccio,H.
  TITLE     Frataxin is essential for extramitochondrial Fe-S cluster proteins
            in mammalian tissues
  JOURNAL   Hum Mol Genet 16 (22), 2651-2658 (2007)
   PUBMED   17597094
REFERENCE   9  (bases 1 to 904)
  AUTHORS   Canal,F., Fosset,C., Chauveau,M.J., Drapier,J.C. and Bouton,C.
  TITLE     Regulation of the cysteine desulfurase Nfs1 and the scaffold
            protein IscU in macrophages stimulated with interferon-gamma and
            lipopolysaccharide
  JOURNAL   Arch Biochem Biophys 465 (1), 282-292 (2007)
   PUBMED   17603005
  REMARK    GeneRIF: While IFN-gamma alone induced Nfs1 protein instability,
            LPS triggered a delayed decline of Nfs1, rather involving
            transcriptional events or mRNA instability.
REFERENCE   10 (bases 1 to 904)
  AUTHORS   Rubtsov,A., Strauch,P., Digiacomo,A., Hu,J., Pelanda,R. and
            Torres,R.M.
  TITLE     Lsc regulates marginal-zone B cell migration and adhesion and is
            required for the IgM T-dependent antibody response
  JOURNAL   Immunity 23 (5), 527-538 (2005)
   PUBMED   16286020
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132939.23.
            
            On May 17, 2018 this sequence version replaced NM_025526.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CK791593.1, BC048409.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               AC132939.23        43171-43314         c
            145-258             AC132939.23        41739-41852         c
            259-369             AC132939.23        40787-40897         c
            370-448             AC132939.23        39266-39344         c
            449-904             AC132939.23        37827-38282         c
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 55.55 cM"
     gene            1..904
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /note="iron-sulfur cluster assembly enzyme"
                     /db_xref="GeneID:66383"
                     /db_xref="MGI:MGI:1913633"
     exon            1..144
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /inference="alignment:Splign:2.1.0"
     exon            145..258
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /inference="alignment:Splign:2.1.0"
     exon            259..369
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /inference="alignment:Splign:2.1.0"
     exon            370..448
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /inference="alignment:Splign:2.1.0"
     exon            449..904
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /inference="alignment:Splign:2.1.0"
     regulatory      870..875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /note="hexamer: AATAAA"
     polyA_site      904
                     /gene="Iscu"
                     /gene_synonym="2310020H20Rik; ISCU2; Nifu; Nifun"
                     /note="major polyA site"
ORIGIN      
        1 gaggtcgacg cagctcaagc cggcaggatg gcggcggcca cgggagctgg ccgtctgagg
       61 cgggcggcgt cggcgctgct gctgcggagc ccgcgcctgc ccgcccggga gctgtcggct
      121 ccggccaggc tctaccacaa gaaggttgtg gatcattatg aaaaccctcg gaacgtggga
      181 tcccttgaca agacatctaa aaatgttgga accggattgg tgggggctcc ggcatgtggt
      241 gacgtcatga aactgcagat ccaggtggat gaaaagggga agattgtgga cgccagattc
      301 aaaacatttg gctgcggctc cgccattgcc tccagctcct tagccacaga gtgggtaaag
      361 gggaaaacgg tggaggaagc cctgaccatc aaaaacaccg acatcgccaa ggagctctgc
      421 ctgccgcctg tgaaactgca ctgctccatg ctggcagaag acgccatcaa ggccgccctg
      481 gctgactaca aactgaagca agagtccaag aaggaggagc cagagaagca gtgagccctg
      541 gagacactcc agccagtcac agcagctgct tcctgccacc ctgcacatca aagaagctac
      601 gcaaaacgca cactattcac cacttgcaca aaatgtgccg ttgattacac ctgatcccgt
      661 gttcctttca gctcacgtgt gtccttagtg cagttagtgt cttgatttag tttgtggcct
      721 gggactgaaa tcagcaccga agtcttaagc tctggagagt gacaaggact tgtacctgac
      781 ctgaggtaga ttgccagcag agcgccttat gctggctatc tgataggatc tattactgag
      841 tatttagcag atctgcatat atatattata ataaaaatgt gagacaatgg ttttctgtac
      901 tgaa
//
