LOCUS       NM_028863                868 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus iron-sulfur cluster assembly 2 (Isca2), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_028863 XM_203592 XM_908230
VERSION     NM_028863.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Beilschmidt,L.K., Ollagnier de Choudens,S., Fournier,M.,
            Sanakis,I., Hograindleur,M.A., Clemancey,M., Blondin,G.,
            Schmucker,S., Eisenmann,A., Weiss,A., Koebel,P., Messaddeq,N.,
            Puccio,H. and Martelli,A.
  TITLE     ISCA1 is essential for mitochondrial Fe4S4 biogenesis in vivo
  JOURNAL   Nat Commun 8, 15124 (2017)
   PUBMED   28492233
  REMARK    GeneRIF: The data suggest that cellular processes with different
            requirements for ISCA1, ISCA2 and ISCA1-ISCA2 complex seem to
            exist.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  AUTHORS   He,M., Lu,Y., Xu,S., Mao,L., Zhang,L., Duan,W., Liu,C., Pi,H.,
            Zhang,Y., Zhong,M., Yu,Z. and Zhou,Z.
  TITLE     MiRNA-210 modulates a nickel-induced cellular energy metabolism
            shift by repressing the iron-sulfur cluster assembly proteins
            ISCU1/2 in Neuro-2a cells
  JOURNAL   Cell Death Dis 5 (2), e1090 (2014)
   PUBMED   24577088
  REMARK    GeneRIF: Data suggest that miR-210 induced repression of ISCU1/2
            may contribute to HIF-1alpha-triggered alterations in neuronal
            energy metabolism after nickel exposure.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK002936.1, BY125913.1 and BY577917.1.
            
            On or before Jul 18, 2009 this sequence version replaced
            XM_908230.2, XM_203592.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK002936.1, BU938850.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AK002936.1         1-17
            18-205              BY125913.1         11-198
            206-864             AK002936.1         205-863
            865-868             BY577917.1         386-389
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 39.38 cM"
     gene            1..868
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /note="iron-sulfur cluster assembly 2"
                     /db_xref="GeneID:74316"
                     /db_xref="MGI:MGI:1921566"
     exon            1..102
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..496
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /note="HESB like domain containing 1; iron-sulfur cluster
                     assembly 2 homolog, mitochondrial; HESB-like
                     domain-containing protein 1"
                     /codon_start=1
                     /product="iron-sulfur cluster assembly 2 homolog,
                     mitochondrial precursor"
                     /protein_id="NP_083139.1"
                     /db_xref="CCDS:CCDS49111.1"
                     /db_xref="GeneID:74316"
                     /db_xref="MGI:MGI:1921566"
                     /translation="MAASRALSLTAEAVRAVIPRRSGRLLAVFPRLLTRWETTSSIPE
                     AGEGQIRLTDSCVQRLLEITEGSEFLRLQVEGGGCSGFQYKFSLDTVINPDDRVFEQG
                     GARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSVKV"
     exon            103..205
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /inference="alignment:Splign:2.1.0"
     exon            206..321
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /inference="alignment:Splign:2.1.0"
     exon            322..868
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      845..850
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /note="hexamer: TATAAA"
     polyA_site      867
                     /gene="Isca2"
                     /gene_synonym="0710001C05Rik; 5730594E03Rik; Hbld1"
                     /note="major polyA site"
ORIGIN      
        1 accatgctag gggcggagct tgcggagtaa gatggcggcc tccagggcct tgtccctaac
       61 tgccgaggcg gtcagggctg tcattcctag gcgctcggga aggctcctcg ccgtctttcc
      121 caggcttttg acccgctggg aaacaacatc ttccattcca gaggctggcg agggacagat
      181 ccgcctcaca gacagctgcg tccagaggct tctggaaatc accgaagggt cagaattcct
      241 caggctgcaa gtagagggag gtggatgctc cggattccaa tacaaatttt cactggatac
      301 agttattaac cccgacgaca gggtatttga acagggtggg gcaagagtgg tggttgactc
      361 tgatagcttg gccttcgtta agggagccca ggtggacttc agccaagaac tgatccgaag
      421 ctcattccaa gtgttgaata accctcaagc ccagcaaggc tgctcctgtg ggtcatcctt
      481 ctctgtcaaa gtctgacaca atgacggatg accctgagat tgccaccact gcaccagtgt
      541 gaacaaccta gaatagtaga attctagagg tttctttctc atcatgtccg gctctcatct
      601 tatttaattt aatccagaag tgtcttattc taccactgta aattatggaa tgtgaagctt
      661 ttaactcttg gccgaaatac tctcccctcc cccagtacaa tgtcaaggcc acatccaaag
      721 gctgttacct cacctgtaat cactggttgg aacccagtat aatttgtagc acctctagga
      781 ctccaaaatt tggagttaac ctctctagcc ttcagaattg tttatatatc tgtgtatagc
      841 tatatataaa acatcattat aaactaag
//
