LOCUS       NM_026921               1915 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus iron-sulfur cluster assembly 1 (Isca1), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_026921
VERSION     NM_026921.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Cheong,A., Archambault,D., Degani,R., Iverson,E., Tremblay,K.D. and
            Mager,J.
  TITLE     Nuclear-encoded mitochondrial ribosomal proteins are required to
            initiate gastrulation
  JOURNAL   Development 147 (10) (2020)
   PUBMED   32376682
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1915)
  AUTHORS   Beilschmidt,L.K., Ollagnier de Choudens,S., Fournier,M.,
            Sanakis,I., Hograindleur,M.A., Clemancey,M., Blondin,G.,
            Schmucker,S., Eisenmann,A., Weiss,A., Koebel,P., Messaddeq,N.,
            Puccio,H. and Martelli,A.
  TITLE     ISCA1 is essential for mitochondrial Fe4S4 biogenesis in vivo
  JOURNAL   Nat Commun 8, 15124 (2017)
   PUBMED   28492233
  REMARK    GeneRIF: The data suggest that cellular processes with different
            requirements for ISCA1, ISCA2 and ISCA1-ISCA2 complex seem to
            exist.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1915)
  AUTHORS   He,M., Lu,Y., Xu,S., Mao,L., Zhang,L., Duan,W., Liu,C., Pi,H.,
            Zhang,Y., Zhong,M., Yu,Z. and Zhou,Z.
  TITLE     MiRNA-210 modulates a nickel-induced cellular energy metabolism
            shift by repressing the iron-sulfur cluster assembly proteins
            ISCU1/2 in Neuro-2a cells
  JOURNAL   Cell Death Dis 5 (2), e1090 (2014)
   PUBMED   24577088
  REMARK    GeneRIF: Data suggest that miR-210 induced repression of ISCU1/2
            may contribute to HIF-1alpha-triggered alterations in neuronal
            energy metabolism after nickel exposure.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1915)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1915)
  AUTHORS   Nilsson,R., Schultz,I.J., Pierce,E.L., Soltis,K.A.,
            Naranuntarat,A., Ward,D.M., Baughman,J.M., Paradkar,P.N.,
            Kingsley,P.D., Culotta,V.C., Kaplan,J., Palis,J., Paw,B.H. and
            Mootha,V.K.
  TITLE     Discovery of genes essential for heme biosynthesis through
            large-scale gene expression analysis
  JOURNAL   Cell Metab 10 (2), 119-130 (2009)
   PUBMED   19656490
  REMARK    GeneRIF: Required for functional heme biosynthesis in erythroid
            cells.
REFERENCE   7  (bases 1 to 1915)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC134869.4.
            
            On Apr 30, 2007 this sequence version replaced NM_026921.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK169028.1, AK045175.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN02415134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-166               AC134869.4         203795-203960       c
            167-220             AC134869.4         196913-196966       c
            221-326             AC134869.4         193061-193166       c
            327-1915            AC134869.4         189586-191174       c
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 31.88 cM"
     gene            1..1915
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
                     /note="iron-sulfur cluster assembly 1"
                     /db_xref="GeneID:69046"
                     /db_xref="MGI:MGI:1916296"
     exon            1..166
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..475
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
                     /EC_number="2.7.7.52"
                     /note="HESB like domain containing 2; iron-sulfur cluster
                     assembly 1 homolog, mitochondrial; HESB-like
                     domain-containing protein 2; iron-sulfur assembly protein
                     IscA"
                     /codon_start=1
                     /product="iron-sulfur cluster assembly 1 homolog,
                     mitochondrial precursor"
                     /protein_id="NP_081197.1"
                     /db_xref="CCDS:CCDS26578.1"
                     /db_xref="GeneID:69046"
                     /db_xref="MGI:MGI:1916296"
                     /translation="MSASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPE
                     HVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYV
                     EDKLSSEFVFNNPNIKGTCGCGESFHV"
     exon            167..220
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
                     /inference="alignment:Splign:2.1.0"
     exon            221..326
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
                     /inference="alignment:Splign:2.1.0"
     exon            327..1915
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      584..589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
                     /note="hexamer: AATAAA"
     polyA_site      609
                     /gene="Isca1"
                     /gene_synonym="1810010A06Rik; Hbld2"
ORIGIN      
        1 gtggcgtcaa agtgcccgcc gtgtccatgg agagaagttg agtccccacg ctccagccgg
       61 aggctgcggg tgccgcgacg tggacatgtc ggcgtcgttg gtccgcgcca ccgtgcgggc
      121 tgtgagcaag agaaaactgc agcccacgcg ggcggccctc acactgaccc cctctgctgt
      181 aaacaagata aaacaacttc ttaaagacaa acctgagcat gtgggtctga aagttggcgt
      241 gcgaaccagg ggctgtaatg gcctctctta cagcctggag tacacaaaga caaaaggaga
      301 ttctgatgaa gaagttattc aagatggagt ccgagtgttc atcgagaaga aagcacagct
      361 aaccctgtta ggaacagaga tggactatgt ggaagacaaa ctgtccagtg agtttgtgtt
      421 caataacccc aacatcaagg gaacctgtgg ctgcggtgag agctttcacg tgtgaaacct
      481 caggactgtg agctccaggt gagccgggtc tgcctcggcg cttcctgaag aaatcatgtg
      541 atggcacgtg ctcaggcgtg tggctgcctg cctcgtgggt gaaaataaag tgacgcattt
      601 gaaaaccaag ccaatgtggg aagagttcca aaagcaatgt ctgtactctc tgtaagacac
      661 aaactgagaa aacatggcgt ctatactctc aaagacacaa actgagaaaa catggcgtct
      721 atactaagac acaactgaga agccgtagat ttccttgagt cattctgttc tctacaggaa
      781 actcctaccc tgcccttgtg ctgtagcctg ctttgtgcta gaaccagctt catggccatt
      841 gctttgctgg gaattgagga atgggataac aggcgtgacc tacatcacag catggcttga
      901 gtgtgtctat ccctttctca atctttgcca acccccgatc tcaggcagga caactgtggg
      961 tcacggtgta gtgggacctc atttccctca cctttggcat ggcccattac atttttacca
     1021 aaaatgtgat gatcacatgc ctcgtttgcc aacccttcaa cagttcctga gttctgagtt
     1081 ccagtatctg aacttgggca ccaccaggcc tgggttagca catctcggtg agcttcatga
     1141 tggtttgctg tagacctttc agttagaaac gcttgtgcta agcagccagc ttgcttgctc
     1201 agttcctgcc cagctctccc ttcctcgagg tcacagcagt gctgtctgag acattcggtg
     1261 aggagagtgg cgatggcttc ccgcctggcc agcgctctca ccagagtttc ttagtttata
     1321 gagacttgtt agttttcttt tgactagact cttgtgtaaa ccctttcgtg tgagttgcca
     1381 tcttgttcgt ttagaacttt gaaacagtag ttacagacat ctgcagcccg ggattcctct
     1441 cagcaccggg cctgtggagc ggaacatgcc attctgacac ttgccagtat gcatatgctt
     1501 tctggttcct aaacgaaatt gctaaatatt tttaaatatt tttatcacca gttttttgag
     1561 ccatggtttt gctgtatact acaggccagc cttgaacgca caatcctcct gcttcaacct
     1621 tctgaggcat gtgctaccat gcctggatcc caagtttttc cttaagtggt cttgattgac
     1681 ttgggttgga catcttaagt aacctgtgaa ttcttttgtg gaaggctgag tctcatgcag
     1741 ctgagtttaa tatttgtgct atttactaaa atacctacca ccaaaatata ccaactcgta
     1801 gttttatatg aatgttgatg agtctgtatc ataaatagaa ttgttgatac atccttaatt
     1861 tgtgcaatat tgtatgaaga gattgagttg tcgattaaaa acatgcatct ttatg
//
