LOCUS       NM_010574               2623 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus Iroquois homeobox 2 (Irx2), mRNA.
ACCESSION   NM_010574 XM_127412
VERSION     NM_010574.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Jasmine, Baraiya,D.H., Kavya,T.T., Mandal,A., Chakraborty,S.,
            Sathish,N., Francis,C.M.R. and Binoy Joseph,D.
  TITLE     Epithelial and mesenchymal compartments of the developing bladder
            and urethra display spatially distinct gene expression patterns
  JOURNAL   Dev Biol 520, 155-170 (2025)
   PUBMED   39798644
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Xu,J., Zhou,X., Zhang,T., Zhang,B. and Xu,P.X.
  TITLE     Smarca4 deficiency induces Pttg1 oncogene upregulation and
            hyperproliferation of tubular and interstitial cells during kidney
            development
  JOURNAL   Front Cell Dev Biol 11, 1233317 (2023)
   PUBMED   37727504
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2623)
  AUTHORS   Ma,Z.G., Yuan,Y.P., Fan,D., Zhang,X., Teng,T., Song,P., Kong,C.Y.,
            Hu,C., Wei,W.Y. and Tang,Q.Z.
  TITLE     IRX2 regulates angiotensin II-induced cardiac fibrosis by
            transcriptionally activating EGR1 in male mice
  JOURNAL   Nat Commun 14 (1), 4967 (2023)
   PUBMED   37587150
  REMARK    GeneRIF: IRX2 regulates angiotensin II-induced cardiac fibrosis by
            transcriptionally activating EGR1 in male mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2623)
  AUTHORS   Huang,R., Zhang,C., Zheng,Y., Zhang,W., Huang,H., Qiu,M., Li,J. and
            Li,F.
  TITLE     ISL1 regulates lung branching morphogenesis via Shh signaling
            pathway
  JOURNAL   J Biol Chem 299 (8), 105034 (2023)
   PUBMED   37442233
REFERENCE   5  (bases 1 to 2623)
  AUTHORS   Puelles,L. and Hidalgo-Sanchez,M.
  TITLE     The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic
            Organizer
  JOURNAL   Int J Mol Sci 24 (11), 9769 (2023)
   PUBMED   37298722
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2623)
  AUTHORS   Peters,T., Dildrop,R., Ausmeier,K. and Ruther,U.
  TITLE     Organization of mouse Iroquois homeobox genes in two clusters
            suggests a conserved regulation and function in vertebrate
            development
  JOURNAL   Genome Res 10 (10), 1453-1462 (2000)
   PUBMED   11042145
REFERENCE   7  (bases 1 to 2623)
  AUTHORS   Christoffels,V.M., Keijser,A.G., Houweling,A.C., Clout,D.E. and
            Moorman,A.F.
  TITLE     Patterning the embryonic heart: identification of five mouse
            Iroquois homeobox genes in the developing heart
  JOURNAL   Dev Biol 224 (2), 263-274 (2000)
   PUBMED   10926765
REFERENCE   8  (bases 1 to 2623)
  AUTHORS   Bosse,A., Stoykova,A., Nieselt-Struwe,K., Chowdhury,K.,
            Copeland,N.G., Jenkins,N.A. and Gruss,P.
  TITLE     Identification of a novel mouse Iroquois homeobox gene, Irx5, and
            chromosomal localisation of all members of the mouse Iroquois gene
            family
  JOURNAL   Dev Dyn 218 (1), 160-174 (2000)
   PUBMED   10822268
REFERENCE   9  (bases 1 to 2623)
  AUTHORS   Cohen,D.R., Cheng,C.W., Cheng,S.H. and Hui,C.C.
  TITLE     Expression of two novel mouse Iroquois homeobox genes during
            neurogenesis
  JOURNAL   Mech Dev 91 (1-2), 317-321 (2000)
   PUBMED   10704856
REFERENCE   10 (bases 1 to 2623)
  AUTHORS   Bosse,A., Zulch,A., Becker,M.B., Torres,M., Gomez-Skarmeta,J.L.,
            Modolell,J. and Gruss,P.
  TITLE     Identification of the vertebrate Iroquois homeobox gene family with
            overlapping expression during early development of the nervous
            system
  JOURNAL   Mech Dev 69 (1-2), 169-181 (1997)
   PUBMED   9486539
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT030229.19, AK148520.1 and AK018266.1.
            
            On Jan 22, 2019 this sequence version replaced NM_010574.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148520.1, BC029750.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-77                CT030229.19        125419-125495
            78-700              AK148520.1         79-701
            701-1300            AK018266.1         105-704
            1301-2623           AK148520.1         1302-2624
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 39.23 cM"
     gene            1..2623
                     /gene="Irx2"
                     /note="Iroquois homeobox 2"
                     /db_xref="GeneID:16372"
                     /db_xref="MGI:MGI:1197526"
     exon            1..494
                     /gene="Irx2"
                     /inference="alignment:Splign:2.1.0"
     CDS             243..1667
                     /gene="Irx2"
                     /note="homeodomain protein IRXA2; iroquois homeobox
                     protein 2; Iroquois related homeobox 2"
                     /codon_start=1
                     /product="iroquois-class homeodomain protein IRX-2"
                     /protein_id="NP_034704.1"
                     /db_xref="CCDS:CCDS26628.1"
                     /db_xref="GeneID:16372"
                     /db_xref="MGI:MGI:1197526"
                     /translation="MSYPQGYLYQAPGSLALYSCPAYGASALAAPRSEELARSASGSA
                     FSPYPGSAAFTAQAATGFGSPLQYSADAAAAAAAGFPSYVGSPYDTHTTGMTGAISYH
                     PYGSAAYPYQLNDPAYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLT
                     QVSTWFANARRRLKKENKMTWAPRNKSEDEDEDEGDASRSKEESSDKAQDGTETSAED
                     EGISLHVDSLTDHSCSAESDGEKLPCRAGDALCESGSECKDKFEDLEDEEDEEDECER
                     DLAPPKPVTSSPLTGVEAPLLSPAPEAAPRGGSGGKTPLGSRTSPGAPPPASKPKLWS
                     LAEIATSDLKQPSLGPGCGPPGLPAAAAPASTGAPPGGSPYSASPLLGRHLYYTSPFY
                     GNYTNYGNLNAALQGQGLLRYNTAASSPGETLHAMPKAASDTGKAGSHSLESHYRPPG
                     GGYEPKKDTSEGCAVVGAGVQTYL"
     exon            495..900
                     /gene="Irx2"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1614
                     /gene="Irx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1690
                     /gene="Irx2"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..2623
                     /gene="Irx2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2269..2274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irx2"
                     /note="hexamer: AATAAA"
     polyA_site      2296
                     /gene="Irx2"
                     /note="major polyA site"
     regulatory      2593..2598
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irx2"
                     /note="hexamer: AAGAAA"
     regulatory      2603..2608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irx2"
                     /note="hexamer: AATAAA"
     polyA_site      2609
                     /gene="Irx2"
     polyA_site      2623
                     /gene="Irx2"
ORIGIN      
        1 actcctgact gcagactccc ccaaatcccg accgccggcc tggcgcgcgg ctgcgcccac
       61 cggctccgcg ctgccgctac cgccgccccg ccgatcctcc agccccgcgc ccagcctagc
      121 gcccaccagc ccagcctgct ccggcccgcg gcctcggtgc gccccggccg ggcgtggatg
      181 gagggcgccg cgcgcgctgc ccgctgctcc gtgtgacgcg ggccccgcgc cccgcgccca
      241 ccatgtccta cccgcagggc tacctgtacc aagcgcccgg ctcgctggcg ctctactcgt
      301 gccccgcgta cggcgcgtcc gcgctggcgg cgccgcgcag cgaggagctg gcgcgttcgg
      361 cgtcgggctc cgcgttcagc ccgtaccccg gctcggcggc cttcacagcg caggcggcaa
      421 ccggcttcgg cagcccgctg cagtactcgg cggacgccgc cgccgccgcg gctgccggct
      481 tcccgtccta cgtgggctcg ccatacgaca cgcacaccac cggaatgact ggcgccatca
      541 gctaccatcc atatggcagt gcggcctatc cataccagct caacgacccc gcgtaccgta
      601 agaacgctac gcgggacgcc actgccacac tgaaagcctg gctcaacgaa caccgaaaga
      661 acccgtaccc caccaaggga gagaagatca tgctggccat tatcaccaag atgaccctca
      721 cgcaggtctc cacctggttc gccaacgcgc gccgacgtct caagaaggag aacaaaatga
      781 cctgggcccc aaggaacaaa agcgaggatg aggacgagga cgagggagat gcttccagaa
      841 gcaaggagga gagttcagac aaggcacagg atggcacaga gacctctgcg gaggacgaag
      901 ggatcagtct acacgtcgac tcgctcacgg accactcgtg ctcagcggag tcagatgggg
      961 aaaaattgcc ctgccgcgcc ggggatgcct tgtgcgaatc aggctcagag tgtaaggaca
     1021 agtttgagga cctggaggac gaggaggacg aggaagacga gtgcgagcgg gacctggcgc
     1081 cacccaagcc cgtaacctcc tcgcctctca ccggcgtgga ggcacccctg ctgagcccgg
     1141 cgcccgaagc cgcgccgcgc ggtggcagcg gtggcaagac gcccctaggc agccggacgt
     1201 cgccaggagc gccgccgccc gccagcaaac ccaagctgtg gtcgctggcc gagatagcca
     1261 cgtcggacct caagcaacca agcttgggcc cgggttgcgg gcctccgggg ctgcctgctg
     1321 ccgccgcgcc tgcctcgact ggggcccctc cgggaggctc gccctactct gcttcgcccc
     1381 tgctgggccg ccacctctat tacacgtccc ctttctatgg caactacaca aactacggga
     1441 acttaaacgc agcgctgcaa ggccagggcc ttctgcggta caataccgcg gcctcctcac
     1501 caggcgagac actgcacgcc atgcccaagg cggccagcga cacgggcaag gcgggctcgc
     1561 attcactgga gtcccactac aggcctccgg gcggcggcta cgagcccaag aaagatacca
     1621 gtgagggctg tgcagttgtt ggtgcaggcg tccagaccta cctataggag ggccaaggtc
     1681 agcagtgcaa gtaggtgtca caattgcttt ggaaaaaaaa gtcaggaggc tattctccct
     1741 tcccaagttc ataaatacac agatacaaac cccagatgtg gaaacccgga ccacattttg
     1801 tgccactgta taagaggatg cacactgcac tgccaaccca cagactctaa ggactggaca
     1861 cacgttgttt gggcatatga gaacttgttg tcatagtata gcttccctat gtttgtgtga
     1921 cttttggaaa caatctgttt tttctcagga tatcctcatc acttcattgc cacggtatat
     1981 attctatagg tgtgatagga cttgctgtaa aatttatttg taaaaaaaaa tgtacacggt
     2041 agaactaacg tgattgaaga acttgagtcc ttcagaagtg gaaacaaatt tgatatttat
     2101 ttttataatg atataaagct tctagtaatt tatgcaagtt gtattgcaat gaaatctaaa
     2161 acttttgtaa ataaattctg cctggttttt atttgaacat tgctggttat acaatctctg
     2221 ttggttgttt aacatcagtt ctcctattaa tttatatcta aaactgtaaa taaaaacaga
     2281 cgcgcctaca agaaaatggt gtttggggct catttttact ggagacccat ttgggtagaa
     2341 gctccagcgg tagccctggt tcctgcccca aaggtggtcc tacctcacct tttaagtgaa
     2401 gagccagtga aataaacgtc cttcttgagc ttatttgaaa aactgcagac ctgagagaaa
     2461 ttaatgtaca acagggagat acctacggtt ccagtttatt cacatagaaa tgtgtttcca
     2521 gaaagtaatt caggtttttt tttgttctct gaatatgcaa atccctactt agtgtaaatt
     2581 tctaaaacat ggaagaaaac aaaataaaag gatatgccac ctc
//
