LOCUS       NM_010570               9163 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus insulin receptor substrate 1 (Irs1), mRNA.
ACCESSION   NM_010570
VERSION     NM_010570.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Basu,S., Mautner,L., Whiting,K., Hasenpusch-Theil,K., Borkowska,M.
            and Theil,T.
  TITLE     Identification of Pappa and Sall3 as Gli3 direct target genes
            acting downstream of cilia signaling in corticogenesis
  JOURNAL   Cereb Cortex 34 (12) (2024)
   PUBMED   39716738
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Yuan,W., Lin,H., Sun,Y., Liu,L., Yan,M., Song,Y., Zhang,X., Lu,X.,
            Xu,Y., He,Q., Ouyang,K., Zhang,C., Pan,Y., Huang,Y., Li,Y., Lu,X.
            and Liu,J.
  TITLE     Myocardin reverses insulin resistance and ameliorates
            cardiomyopathy by increasing IRS-1 expression in a murine model of
            lipodystrophy caused by adipose deficiency of vacuolar H+-ATPase
            V0d1 subunit
  JOURNAL   Theranostics 14 (5), 2246-2264 (2024)
   PUBMED   38505620
  REMARK    GeneRIF: Myocardin reverses insulin resistance and ameliorates
            cardiomyopathy by increasing IRS-1 expression in a murine model of
            lipodystrophy caused by adipose deficiency of vacuolar H[+]-ATPase
            V0d1 subunit.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9163)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 9163)
  AUTHORS   Qu,Z., Shi,L., Wu,Z., Lin,P., Zhang,G., Cong,X., Zhao,X., Ge,H.,
            Yan,S., Jiang,L. and Wu,H.
  TITLE     Kinesin light chain 1 stabilizes insulin receptor substrate 1 to
            regulate the IGF-1-AKT signaling pathway during myoblast
            differentiation
  JOURNAL   FASEB J 38 (3), e23432 (2024)
   PUBMED   38300173
  REMARK    GeneRIF: Kinesin light chain 1 stabilizes insulin receptor
            substrate 1 to regulate the IGF-1-AKT signaling pathway during
            myoblast differentiation.
REFERENCE   5  (bases 1 to 9163)
  AUTHORS   Wei,X., Wang,J., Sun,Y., Zhao,T., Luo,X., Lu,J., Hou,W., Yu,X.,
            Xue,L., Yan,Y. and Wang,H.
  TITLE     MiR-222-3p suppresses C2C12 myoblast proliferation and
            differentiation via the inhibition of IRS-1/PI3K/Akt pathway
  JOURNAL   J Cell Biochem 124 (9), 1379-1390 (2023)
   PUBMED   37565526
  REMARK    GeneRIF: MiR-222-3p suppresses C2C12 myoblast proliferation and
            differentiation via the inhibition of IRS-1/PI3K/Akt pathway.
REFERENCE   6  (bases 1 to 9163)
  AUTHORS   Furusaka,A., Nishiyama,M., Nishimaki,H., Ogasawara,Y., Tamemoto,H.,
            Yamauchi,T., Tobe,K., Kadowaki,T. and Tanaka,T.
  TITLE     Ornithine decarboxylase induction during liver regeneration in
            IRS-1-deficient mice
  JOURNAL   Biochem Biophys Res Commun 216 (1), 284-290 (1995)
   PUBMED   7488102
REFERENCE   7  (bases 1 to 9163)
  AUTHORS   Yin,T., Keller,S.R., Quelle,F.W., Witthuhn,B.A., Tsang,M.L.,
            Lienhard,G.E., Ihle,J.N. and Yang,Y.C.
  TITLE     Interleukin-9 induces tyrosine phosphorylation of insulin receptor
            substrate-1 via JAK tyrosine kinases
  JOURNAL   J Biol Chem 270 (35), 20497-20502 (1995)
   PUBMED   7544789
REFERENCE   8  (bases 1 to 9163)
  AUTHORS   Pons,S., Asano,T., Glasheen,E., Miralpeix,M., Zhang,Y.,
            Fisher,T.L., Myers,M.G. Jr., Sun,X.J. and White,M.F.
  TITLE     The structure and function of p55PIK reveal a new regulatory
            subunit for phosphatidylinositol 3-kinase
  JOURNAL   Mol Cell Biol 15 (8), 4453-4465 (1995)
   PUBMED   7542745
REFERENCE   9  (bases 1 to 9163)
  AUTHORS   Tobe,K., Tamemoto,H., Yamauchi,T., Aizawa,S., Yazaki,Y. and
            Kadowaki,T.
  TITLE     Identification of a 190-kDa protein as a novel substrate for the
            insulin receptor kinase functionally similar to insulin receptor
            substrate-1
  JOURNAL   J Biol Chem 270 (11), 5698-5701 (1995)
   PUBMED   7534300
REFERENCE   10 (bases 1 to 9163)
  AUTHORS   Araki,E., Lipes,M.A., Patti,M.E., Bruning,J.C., Haag,B. 3rd,
            Johnson,R.S. and Kahn,C.R.
  TITLE     Alternative pathway of insulin signalling in mice with targeted
            disruption of the IRS-1 gene
  JOURNAL   Nature 372 (6502), 186-190 (1994)
   PUBMED   7526222
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC137845.9 and AC123690.19.
            
            On Nov 27, 2007 this sequence version replaced NM_010570.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045317.1, X69722.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4663              AC137845.9         102444-107106
            4664-9163           AC123690.19        7046-11545
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 42.0 cM"
     gene            1..9163
                     /gene="Irs1"
                     /gene_synonym="G972R; IRS-1"
                     /note="insulin receptor substrate 1"
                     /db_xref="GeneID:16367"
                     /db_xref="MGI:MGI:99454"
     exon            1..4663
                     /gene="Irs1"
                     /gene_synonym="G972R; IRS-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             947..4642
                     /gene="Irs1"
                     /gene_synonym="G972R; IRS-1"
                     /codon_start=1
                     /product="insulin receptor substrate 1"
                     /protein_id="NP_034700.2"
                     /db_xref="CCDS:CCDS15096.1"
                     /db_xref="GeneID:16367"
                     /db_xref="MGI:MGI:99454"
                     /translation="MASPPDTDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARL
                     EYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAE
                     QDSWYQALLQLHNRAKAHHDGAGGGCGGSCSGSSGVGEAGEDLSYDTGPGPAFKEVWQ
                     VILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFI
                     EVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSSCSNPIS
                     VPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRP
                     ASVDGSPVSPSTNRTHAHRHRGSSRLHPPLNHSRSIPMPSSRCSPSATSPVSLSSSST
                     SGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPP
                     ARGEEELSNYICMGGKGASTLAAPNGHYILSRGGNGHRYIPGANLGTSPALPGDEAAG
                     AADLDNRFRKRTHSAGTSPTISHQKTPSQSSVASIEEYTEMMPAAYPPGGGSGGRLPG
                     YRHSAFVPTHSYPEEGLEMHHLERRGGHHRPDTSNLHTDDGYMPMSPGVAPVPSNRKG
                     NGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGSCSPDIGGGSSSSSSIS
                     AAPSGSSYGKPWTNGVGGHHTHALPHAKPPVESGGGKLLPCTGDYMNMSPVGDSNTSS
                     PSECYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSSSSGRLRYTAT
                     AEDSSSSTSSDSLGGGYCGARPESSLTHPHHHVLQPHLPRKVDTAAQTNSRLARPTRL
                     SLGDPKASTLPRVREQQQQQQSSLHPPEPKSPGEYVNIEFGSGQPGYLAGPATSRSSP
                     SVRCPPQLHPAPREETGSEEYMNMDLGPGRRATWQESGGVELGRIGPAPPGSATVCRP
                     TRSVPNSRGDYMTMQIGCPRQSYVDTSPVAPVSYADMRTGIAAEKASLPRPTGAAPPP
                     SSTASSSVTPQGATAEQATHSSLLGGPQGPGGMSAFTRVNLSPNHNQSAKVIRADTQG
                     CRRRHSSETFSAPTRAGNTVPFGAGAAVGGSGGGGGGGSEDVKRHSSASFENVWLRPG
                     DLGGVSKESAPVCGAAGGLEKSLNYIDLDLAKERSQDCPSQQQSLPPPPPHQPLGSNE
                     GNSPRRSSEDLSNYASISFQKQPEDRQ"
     exon            4664..9163
                     /gene="Irs1"
                     /gene_synonym="G972R; IRS-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9144..9149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irs1"
                     /gene_synonym="G972R; IRS-1"
                     /note="hexamer: AAGAAA"
     polyA_site      9163
                     /gene="Irs1"
                     /gene_synonym="G972R; IRS-1"
                     /note="major polyA site"
ORIGIN      
        1 ggaggaggaa ggaggaggga ggaggaggag ggaggagaag gagggaggag gagagaagta
       61 actgcagcgg ctgcgcctcg ggaggaccgg gcgtcttccc cgatctcttc ccaaacctcc
      121 cccgctcccc gcctccctcc ctcgtcccct cccctcctcc cttgcggact ggagctaggg
      181 gcagggatga gcctgtccct ctggaccgtc ccctgctccc ccagctgccc agtagcgttg
      241 cgcgtggaaa agccactttc cccgcccgcc gagatgggct cgagtgcggg ctgcagagga
      301 cgcgtcctcg ggcggcagca gcagcagcag cagcagcaac agccgcagcg ccgcggtctc
      361 tgcgattgag ctggtatttg ggcggctggt ggcgacgggg acggttgaag ggtgggagga
      421 ggcagaggag gaggaggaga aggaggaggg agaacccggt gcaacgttgg gacttggcag
      481 cccgcctccc cctgcccaag gatatttaat ttgcctcggg aatcgctact tccagagggg
      541 aactcgggag ggaaggcgcg cgcgcctaga gagccaagcg gggactcctc gggccatcgc
      601 tgcctcccta catcggactc gaccaggggc ggcaagggat gcaccatagc tccttctctg
      661 ctgcagggac tgggggagac atagtcctcg gaagattgcg gctgcactca ccctagaccc
      721 actgcccttc cctctgggca tgaaacgccc ttaaactcgg atcaggctat cttccttggc
      781 gcagttacct cgtccttcgg ctgcccctcc ccagctccag gaacagcgtg aattttggag
      841 tcagaatttc tgcgagcttc ctccagcccg gagtgcatgt gcggggccgc actgagaagc
      901 cacccctcac ccagtttttc aacacctccc tctgctccgc agcagcatgg cgagccctcc
      961 ggataccgat ggcttctcag acgtgcgcaa ggtgggctac ctgcgcaagc ccaagagtat
     1021 gcataagcgc tttttcgtgc tgcgggcggc cagcgaggcc gggggcccag cgcgcctgga
     1081 gtattatgag aacgagaaga agtggcggca caagtcgagc gcccccaaac gctcgatccc
     1141 cctcgagagc tgtttcaaca tcaacaagcg ggctgactcc aagaacaagc acctggtggc
     1201 tctctacacc cgagacgaac actttgccat tgccgcggat agcgaggctg agcaagacag
     1261 ctggtaccag gctcttctgc agctgcataa tcgggcaaag gcccaccatg acggggctgg
     1321 aggaggctgc ggcggtagct gcagcggcag ctctggcgtt ggagaggcag gggaggactt
     1381 gagctatgac acgggtccag gacccgcgtt caaggaggtc tggcaggtta tcctgaaacc
     1441 caagggcctg ggtcagacaa agaacctgat tggcatctac cgcctctgcc tgaccagcaa
     1501 gaccatcagc tttgtgaagc tgaactccga ggcagccgct gtggtgctgc agctgatgaa
     1561 tatcagacgc tgtggccact cagagaactt cttcttcatc gaggtggggc gttccgctgt
     1621 gacagggcct ggcgagttct ggatgcaagt ggatgactcc gtggtggccc agaacatgca
     1681 tgagaccatt ctagaggcaa tgagggccat gagcgatgag tttcgccctc gcagcaaaag
     1741 ccagtcttca tccagttgct ccaaccccat cagcgttccc ctgcgcaggc accatctcaa
     1801 caaccctcca cccagccagg tgggactgac tcggagatct cgaactgaga gcatcactgc
     1861 cacctcccct gccagtatgg tgggtgggaa accaggttcc ttccgggtgc gtgcctccag
     1921 cgatggcgaa ggcaccatgt cccgtccagc atcagtggat ggcagtcctg tgagccctag
     1981 caccaacagg acccacgccc atcggcatcg aggcagctcc aggctgcacc ccccactcaa
     2041 ccacagccgc tccatcccca tgccttcttc tagatgctca ccttcagcca ccagcccagt
     2101 gagtctgtca tctagtagta ccagtggcca tggctccact tcagactgtc tcttcccgag
     2161 gcgctctagt gcttccgtgt ccggttcgcc tagcgatggc ggtttcatct cttctgatga
     2221 gtatggttct agtccctgcg atttccgaag ttccttccgc agtgtcaccc cagattccct
     2281 ggggcacacc ccaccagcca ggggtgagga ggagctgagc aattatatct gcatgggtgg
     2341 caagggagcc tccaccttgg ctgctcccaa tggccactac attttgtcta ggggtggcaa
     2401 cggccatcgc tacatcccag gtgctaactt ggggacaagc ccagcgctgc ctggagatga
     2461 agccgcgggt gcagcagatc tggataaccg gtttcgaaag agaactcact ccgcaggcac
     2521 atctcctacc atttcccatc agaagacccc ctcacagtct tcagtggctt ctattgagga
     2581 atatacagag atgatgcccg ctgcctaccc accaggaggt ggcagtggag gccgactgcc
     2641 cggctaccgg cattccgcct tcgtgcccac ccactcctat cccgaagagg gtctagagat
     2701 gcaccacttg gaacgtcgtg gaggccacca ccgtccagac acctccaacc tccacactga
     2761 tgatggctat atgcccatgt ctcctggggt ggctccagtg cccagcaacc gcaaaggaaa
     2821 tggggactat atgcccatga gccccaagag tgtatctgcc ccacagcaga tcattaaccc
     2881 catcagacgc cacccacaga gagtggaccc caatggctac atgatgatgt cacccagtgg
     2941 tagttgctcc cctgacattg gaggtgggtc cagcagcagt agcagcatca gcgcagcccc
     3001 ttctgggagc agctatggga agccatggac aaatggagta ggggggcacc atactcatgc
     3061 ccttcctcat gccaaacctc ctgttgagag tggtggcggt aagctcttgc cttgcacagg
     3121 tgactacatg aacatgtccc cagtgggaga ttccaacacc agcagcccct cagaatgcta
     3181 ctatggccca gaagatcccc agcacaagcc ggtcctctct tactactcat tgccaaggtc
     3241 ctttaagcac acccagcgcc ctggagagcc agaggagggt gccaggcacc agcatcttcg
     3301 tctctcttct agctctggac gccttcgcta taccgcaact gccgaagatt cctcctcttc
     3361 taccagcagc gacagtctgg gtgggggtta ctgtggggct aggccagagt caagcctcac
     3421 acatccccac caccatgtct tgcagcccca cctgcctcga aaggtagaca cagctgcaca
     3481 gaccaacagc cgcctggctc gacccacaag gctgtccttg ggggatccca aggcaagcac
     3541 cttaccccgg gttcgagagc agcaacagca gcagcagtct tccctgcacc ctcccgagcc
     3601 caaaagccca ggagaatatg tgaatattga attcgggagc ggccagcctg gctatttagc
     3661 tggccctgca acttcccgta gctctccttc agttcgatgt ccaccccagc tccacccagc
     3721 tcctagagaa gagactggct cggaagagta catgaacatg gacttggggc caggccggag
     3781 ggcaacctgg caggagagtg gtggagttga gttgggcaga ataggccctg cacctccggg
     3841 gtctgctacg gtttgcaggc caacccgttc ggtgccaaat agccgtggtg actacatgac
     3901 catgcagata ggttgtcctc gtcaaagcta tgtggatacc tcaccagtgg ccccagtcag
     3961 ctatgctgac atgcggacag gcattgctgc agagaaggcg agcctgccta gacccacagg
     4021 agctgctcct cctccatcct ccacagcctc ttcttctgtt acacctcaag gagccaccgc
     4081 tgagcaagct actcactctt ccttgctggg aggccctcag ggacctgggg gcatgagtgc
     4141 attcaccagg gtgaacctca gtcccaacca taaccagagt gccaaagtga ttcgcgcaga
     4201 cactcaaggg tgccggagga ggcatagctc tgagaccttc tcagcaccta ctcgggctgg
     4261 caatacggtg ccctttggag caggggctgc agtagggggc agcggcggtg gtggtggtgg
     4321 cggcagtgag gatgtaaaac gccacagctc tgcatccttt gagaatgtgt ggctgagacc
     4381 tggggatcta gggggagtct ccaaggagtc ggctccagtg tgtggggctg ctgggggttt
     4441 ggagaagagt cttaactaca tagacctgga tttggccaag gagcgctctc aggactgccc
     4501 ctctcaacag cagtccctac caccccctcc ccctcaccag cccttaggca gcaatgaggg
     4561 caactcccca agacgctcca gtgaggattt aagcaactat gccagcatca gcttccagaa
     4621 gcagccagag gatcgtcaat agcgtaactg gacatcacag cagaatgaag acctaaatga
     4681 cctcagcaaa tcctcctttt aactcatggg tacccagact cgaactattt cacaattcac
     4741 aaccaggacc tcacgtcttc ctcttcagta gatggtacga tgcatccgtt acagttttgt
     4801 ttaccttgta caatcctcag gagttcattg actgaactgc acgttctaca ttgtgccaag
     4861 caacaagaaa gcactgtgac accggaacaa ttagtgtgca taaacttcat cttgaacttt
     4921 aaggacagct ggtcacgaag agctggtgca cttcctacca ccaagagaat gggtttactc
     4981 tcatcaaatt tgcaaccgac gtttcctcta ctctgaaacc gtgttccatg atgcgcctct
     5041 gtaaattact ccatatggaa ctgttcatgt tgggtggaga gagtattaaa tatttaacat
     5101 aggttttgat ttatatatgt aattttttta atgaaaatgt agctttcatt acagcacatt
     5161 tttttttttt tttttttgat gtggaactga ggtgttcaat gttctgtttt aaagagtgga
     5221 atgaatgctt aaaacaaggc taaaaagagt agactagggg atgatccttg ttttcaggtt
     5281 ctaattcaga aaaataatat aataagaatc atagtgccat agaaggttct ggactgtata
     5341 gttgtacttg ctgatattgt ctcttgtaat ataaacttga tattgagctg agttcctttt
     5401 aagaattaag cttagttttg tatgttgttt ttttgttttg tttttttttt tcccaaacag
     5461 aaggaggatg tattctactg gggtgttttc aagtgtcggc ttagaattgg aagttgaatg
     5521 gaagcgaact ataacaaaga gaggaaacca cagacttcca ttgtaaatac tgtagagaga
     5581 aggagacatg agcaatccct tcaagtcaaa agctctcctc ggaatgaaga atgtgggtgt
     5641 ttataaattc tgaaaatgtc tttctgttca taatactaga cactgttgga ccctcccccc
     5701 acttctataa gcttttaccc ccacccaccc tcatcctttt tttctgtgta catatcatgt
     5761 ttcatttcat acattgcctc cgagaaggct caagagacaa gtgcattctt gctaactgga
     5821 aattaaaatt ccagattttt tttttttttt gttttgtttt ttaagtcatg accagggttc
     5881 tgtgttttgt tccctaagac acctaccttg tgtgttctgg gaactttccg tgttatgtgt
     5941 gtttgaaatg aatgcaattg gcccatttgc cctggtgttt gctggccttt tagtggttag
     6001 ctaataaaac acagtttaca gagagcacat aaaaacttag tgagctgctg ttgaaacatc
     6061 tagttaggag tgaaaatgtg aatcctttcc ttgtttgaaa ggtcgataga tcttggaact
     6121 ttttacttta tccagcagaa atggcaatga ctatacctga aggtgtttcc tctggaactg
     6181 taaattgtct ttgccttgaa actccctgaa atgacagtga agattcaatg atcaggctcc
     6241 ctttgagtcc ctgtgaactc agccctgcag cagggaggag caacactcta cttctgggaa
     6301 gggttgagcc tcctgggtgg ggctgcaagg aaggagcaag ggttgggagg cactgcagtt
     6361 gcccagttgc cctgatcaac tggttgggga cccctggctc tattcaaaga gataaataga
     6421 cccccaactg ctgaggttcg ggttagtgtt cagtcaacca gatgtacaat gttcagtgtt
     6481 gcagatctta atcatttgca tgctcatcaa tttctaagat aaataggtct ctagtcataa
     6541 gaattcattg ttttcaagga actcgccaaa tgacatcatt tgttgtcagt tagaaaggct
     6601 ttgaaaatag gaagtgaaag ttggtttttt tacaaatgta tgagaaaaga ctctcattgt
     6661 taaagtgact ttttaatttt tttaagcatt ctgactcaaa ttcttgctaa ggtaagaaga
     6721 aagcaagccg cttgctctct gccctcaagg atttgagtta ctaaaaagag accagtgaac
     6781 aatgtcagcc ccaaccattt gcctgtaaag ctggctggag tttttcaagg ggcctaggct
     6841 ttgaatattt atgactaacg cacctctgca tgtggggtta ttgacacttg aggtgagaac
     6901 aggaacaaac agactggaca atcgtctcct ggtttctatc tcccttgctt tgtattgagt
     6961 cagtagtgta aaggtagcct ttcttgttca gcttcttcta gactttaccc gagctggggt
     7021 tgatgaaagc atcaaacttg gctgacttag aactggaagt tgctggtacc caggctgaaa
     7081 tctcttgcgc tggcttcttg aagggtttcc tttaagcaca tcagctgcta agtttttgtg
     7141 ttcaattcct attaaagggg gttggccttt atctatttcc aagaatttgc aggccaacca
     7201 agggcttatg ttaatggggc ttctagtggc ttctagctaa cctggaagtt agtggctttt
     7261 tttctttatg agaccaacct ttcacagcat tcttttttaa agacttgtta agacagggtt
     7321 tttaaattct tctttgtgaa aagaacaggt atgctccctt ggctatgatg atagctttct
     7381 tttgggcgaa agcatagccc tagaaccagc tggaaatgaa gggactccat taatgatcaa
     7441 ccgtctggat caataatgac aaatcagtac tgatgctaca tgtgtggcta tttcttgcta
     7501 actttcaaag gtcagggaca ctcttgacta acttaccaac tacaaacgta gatctactca
     7561 agaatcatca agccagaagt agagagcttt atcctgtctt tccttggcat ttgtctttta
     7621 gctctcattt atatgtgaga caaagtgtta agaaacaatc ttactgtact ataggaaaca
     7681 atattttata gtgtggaaac cctatttgat acagtggaac atcctgaaag caatatacca
     7741 tgtatttgga tacctttaaa atgggtgact taaagatagg ggaagatggc ttaccacatg
     7801 gtgacagtaa gagccatgac agatagtaca gttgtgaagt gctgtggcta ggggtgataa
     7861 tgatcccata tttacctcat gctgccttgg ctgagctcgc taactgagat agtcatacaa
     7921 agaggtccta taccaggaaa ctgacattgg ggagaaaagg attttttgga aggcatatgg
     7981 aggacaactc ggggaaccaa gtaagaacta gtttcttggt agtgacagga caggggggct
     8041 agtgctgtca tgacaaggtg gatgttagca ggaggtgatg ctctcctcac ctgctctggc
     8101 tgcttgaaag tcttgaaaga agcagggtgt ccttccaggt gtgtgagcag gcacaacctt
     8161 gtcagtagta acaaaaagac ttccatgctg ggctggggtt cttagctatg gagagtaaag
     8221 acagccttcc atatagttag tagaaccaag cattaaacac tgggcctctg gtcatccctg
     8281 agtcaagcct gagatgatga tggttcagtc tcttcctcat gcctggagag agtatggcac
     8341 agctagtgtg ttgggtatct atcactcact atttgcagct agaacaccaa atagatgctg
     8401 gaaggagact gagaggttct ggacgtgttt ggccctactc ttaattaaag agcctataat
     8461 tagcaaatat ggtttttaag catctgactt gggtaaggct ttatttccct tccctagctt
     8521 atccatgatg gcttggcatt tgggatgtcc acaccaagag atgggtatgt tatccctttg
     8581 ccatccacct gctatgtaaa cctctctttg tccacgctgt ccctttctcc agcactaacc
     8641 attatgccat accgtagaac aaattcacta cccaggaaaa tgtgtctgtt tgcatactca
     8701 tctgtagtgc tagcattttt attttagtcc attttagaac tttgccactg gcctaagagt
     8761 atagttttca gaataatttt tcttatgtag ttgttttcct tatacctctt ggctacaggg
     8821 tgggccaaac gaatgctatc tctctttcca tttgctgtgt gtggtttggt ttatcatctg
     8881 aagatggtgg tgctatcgac ggtgctactt catctgtgta caaaagaccg acgcatggtc
     8941 tatgttgcta ccaaaacatt tactgtatat atgtggataa catattatgt atatatgtaa
     9001 tgggtaccag gccaggttta tatttttttc tttagacgtg tttcactttt ccaagctttc
     9061 tttatagtgt tatgctcgtg ttaaattctt ttacccccaa ttctgtctgg tacttagaat
     9121 tgtagtgtct tcatcgttaa ttaaagaaaa ccatctaaat gaa
//
