LOCUS       NM_008320               2855 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus interferon regulatory factor 8 (Irf8), transcript
            variant 2, mRNA.
ACCESSION   NM_008320
VERSION     NM_008320.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Saeki,K., Pan,R., Lee,E., Kurotaki,D. and Ozato,K.
  TITLE     IRF8 defines the epigenetic landscape in postnatal microglia,
            thereby directing their transcriptome programs
  JOURNAL   Nat Immunol 25 (10), 1928-1942 (2024)
   PUBMED   39313544
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Yamasaki,A., Imanishi,I., Tanaka,K., Ohkawa,Y., Tsuda,M. and
            Masuda,T.
  TITLE     IRF8 and MAFB drive distinct transcriptional machineries in
            different resident macrophages of the central nervous system
  JOURNAL   Commun Biol 7 (1), 896 (2024)
   PUBMED   39043941
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2855)
  AUTHORS   Jorssen,J., Van Hulst,G., Mollers,K., Pujol,J., Petrellis,G.,
            Baptista,A.P., Schetters,S., Baron,F., Caers,J., Lambrecht,B.N.,
            Dewals,B.G., Bureau,F. and Desmet,C.J.
  TITLE     Single-cell proteomics and transcriptomics capture eosinophil
            development and identify the role of IL-5 in their lineage transit
            amplification
  JOURNAL   Immunity 57 (7), 1549-1566 (2024)
   PUBMED   38776917
REFERENCE   4  (bases 1 to 2855)
  AUTHORS   Gelineau,A., Marcelin,G., Ouhachi,M., Dussaud,S., Voland,L.,
            Manuel,R., Baba,I., Rouault,C., Yvan-Charvet,L., Clement,K.,
            Tussiwand,R., Huby,T. and Gautier,E.L.
  TITLE     Fructooligosaccharides benefits on glucose homeostasis upon
            high-fat diet feeding require type 2 conventional dendritic cells
  JOURNAL   Nat Commun 15 (1), 5413 (2024)
   PUBMED   38926424
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2855)
  AUTHORS   Xiang,M., Wang,L., Guo,S., Lu,Y.Y., Lei,H., Jiang,D.S., Zhang,Y.,
            Liu,Y., Zhou,Y., Zhang,X.D. and Li,H.
  TITLE     Interferon regulatory factor 8 protects against cerebral
            ischaemic-reperfusion injury
  JOURNAL   J Neurochem 129 (6), 988-1001 (2014)
   PUBMED   24528256
  REMARK    GeneRIF: we found that the transcriptional factor IRF8 plays a
            protective role in the cerebral ischaemic-reperfusion injury by
            attenuating neuronal apoptosis
REFERENCE   6  (bases 1 to 2855)
  AUTHORS   Scharton-Kersten,T., Contursi,C., Masumi,A., Sher,A. and Ozato,K.
  TITLE     Interferon consensus sequence binding protein-deficient mice
            display impaired resistance to intracellular infection due to a
            primary defect in interleukin 12 p40 induction
  JOURNAL   J Exp Med 186 (9), 1523-1534 (1997)
   PUBMED   9348310
REFERENCE   7  (bases 1 to 2855)
  AUTHORS   Fehr,T., Schoedon,G., Odermatt,B., Holtschke,T., Schneemann,M.,
            Bachmann,M.F., Mak,T.W., Horak,I. and Zinkernagel,R.M.
  TITLE     Crucial role of interferon consensus sequence binding protein, but
            neither of interferon regulatory factor 1 nor of nitric oxide
            synthesis for protection against murine listeriosis
  JOURNAL   J Exp Med 185 (5), 921-931 (1997)
   PUBMED   9120398
REFERENCE   8  (bases 1 to 2855)
  AUTHORS   Holtschke,T., Lohler,J., Kanno,Y., Fehr,T., Giese,N.,
            Rosenbauer,F., Lou,J., Knobeloch,K.P., Gabriele,L., Waring,J.F.,
            Bachmann,M.F., Zinkernagel,R.M., Morse,H.C. 3rd, Ozato,K. and
            Horak,I.
  TITLE     Immunodeficiency and chronic myelogenous leukemia-like syndrome in
            mice with a targeted mutation of the ICSBP gene
  JOURNAL   Cell 87 (2), 307-317 (1996)
   PUBMED   8861914
REFERENCE   9  (bases 1 to 2855)
  AUTHORS   Kanno,Y., Kozak,C.A., Schindler,C., Driggers,P.H., Ennist,D.L.,
            Gleason,S.L., Darnell,J.E. Jr. and Ozato,K.
  TITLE     The genomic structure of the murine ICSBP gene reveals the presence
            of the gamma interferon-responsive element, to which an ISGF3 alpha
            subunit (or similar) molecule binds
  JOURNAL   Mol Cell Biol 13 (7), 3951-3963 (1993)
   PUBMED   8321202
REFERENCE   10 (bases 1 to 2855)
  AUTHORS   Driggers,P.H., Ennist,D.L., Gleason,S.L., Mak,W.H., Marks,M.S.,
            Levi,B.Z., Flanagan,J.R., Appella,E. and Ozato,K.
  TITLE     An interferon gamma-regulated protein that binds the
            interferon-inducible enhancer element of major histocompatibility
            complex class I genes
  JOURNAL   Proc Natl Acad Sci U S A 87 (10), 3743-3747 (1990)
   PUBMED   2111015
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK150370.1, AK151324.1,
            AK018533.1 and BF662476.1.
            
            On Sep 17, 2014 this sequence version replaced NM_008320.3.
            
            Summary: The protein encoded by this gene is a transcription factor
            that belongs to the interferon regulatory factor family. Proteins
            belonging to this family have a DNA binding domain at the amino
            terminus that contains five well-conserved tryptophan-rich repeats.
            This domain recognizes DNA sequences similar to the
            interferon-stimulated response element. The protein encoded by this
            gene promotes or suppresses lineage-specific genes to regulate the
            differentation of lymphoid and myeloid lineage cells. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Sep 2014].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005450.1, AK036488.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-354               AK150370.1         2-355
            355-363             AK151324.1         356-364
            364-2452            AK150370.1         365-2453
            2453-2654           AK018533.1         2454-2655
            2655-2855           BF662476.1         1-201               c
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 70.05 cM"
     gene            1..2855
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /note="interferon regulatory factor 8"
                     /db_xref="GeneID:15900"
                     /db_xref="MGI:MGI:96395"
     exon            1..52
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     exon            53..227
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..1328
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /note="interferon consensus sequence binding protein 1;
                     interferon consensus sequence-binding protein"
                     /codon_start=1
                     /product="interferon regulatory factor 8"
                     /protein_id="NP_032346.1"
                     /db_xref="CCDS:CCDS22721.1"
                     /db_xref="GeneID:15900"
                     /db_xref="MGI:MGI:96395"
                     /translation="MCDRNGGRRLRQWLIEQIDSSMYPGLIWENDEKTMFRIPWKHAG
                     KQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTDRSQLD
                     ISEPYKVYRIVPEEEQKCKLGVAPAGCMSEVPEMECGRSEIEELIKEPSVDEYMGMTK
                     RSPSPPEACRSQILPDWWVQQPSAGLPLVTGYAAYDTHHSAFSQMVISFYYGGKLVGQ
                     ATTTCLEGCRLSLSQPGLPKLYGPDGLEPVCFPTADTIPSERQRQVTRKLFGHLERGV
                     LLHSNRKGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTNQFIRELQQFYA
                     TQSRLPDSRVVLCFGEEFPDTVPLRSKLILVQVEQLYARQLVEEAGKSCGAGSLMPAL
                     EEPQPDQAFRMFPDICTSHQRPFFRENQQITV"
     exon            228..411
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     exon            412..500
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     exon            501..606
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     exon            607..654
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     exon            655..1035
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1151
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..2847
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2821..2826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /note="hexamer: ATTAAA"
     polyA_site      2847
                     /gene="Irf8"
                     /gene_synonym="ICSBP; Icsbp1; IRF-8; Myls"
                     /note="major polyA site"
ORIGIN      
        1 aggagcccgc gggcggtccc ggaggcgcgg gcagcgtggg aaccggcggc aggatgtgtg
       61 accggaacgg cgggcggcgg ctgcggcagt ggctgatcga acagatcgac agcagcatgt
      121 acccggggct gatctgggaa aatgatgaga agaccatgtt ccgtatcccc tggaagcatg
      181 ccggcaagca ggattacaat caggaggtgg atgcttccat cttcaaggcc tgggcagttt
      241 ttaaagggaa gtttaaagag ggagacaaag ctgaaccagc cacgtggaag acgaggttac
      301 gctgtgctct gaacaagagc ccagattttg aagaagtgac tgaccggtcc cagctggaca
      361 tttctgagcc atataaagtt taccgaattg tccccgagga agaacaaaaa tgcaagctgg
      421 gcgtggcacc tgcaggctgc atgagcgaag ttcctgagat ggagtgtggc cgctcagaga
      481 ttgaggagct gatcaaggaa ccttctgtgg atgagtacat gggtatgacc aagaggagcc
      541 catccccacc agaggcctgc aggagccaga tcctccctga ctggtgggtc cagcagccca
      601 gtgcaggcct gccactggtg accggatatg ccgcctatga cacacaccat tcagctttct
      661 cccagatggt catcagcttc tactacgggg gcaagctggt gggccaggcc accaccacct
      721 gccttgaagg ctgccgtctc tccctgagcc agccggggct gcctaagttg tatgggccgg
      781 atggcctgga acccgtgtgc tttccgacgg ccgacaccat ccccagtgag cggcagaggc
      841 aggtgacccg gaagctgttt gggcacctgg aacgtggcgt gctactgcac agcaaccgca
      901 agggcgtgtt cgtgaagcgg ctgtgccagg gccgcgtgtt ctgcagcggc aacgcggtgg
      961 tgtgcaaggg caggcccaac aagctggagc gggacgaggt ggtgcaggtc tttgacacca
     1021 accagttcat ccgagagctg cagcaattct acgccaccca gagccgccta cctgacagca
     1081 gggtggtcct gtgcttcggg gaggagtttc cggacactgt gcccttgcgc tccaaactca
     1141 ttctggtgca ggtagagcag ctgtatgcca ggcagctggt ggaggaagcg ggcaagagct
     1201 gcggtgctgg ctccctgatg ccagccctgg aggagcccca gccggaccag gctttccgca
     1261 tgtttccgga tatctgtacc tcacaccaga gacccttttt tagagaaaat caacagatca
     1321 ccgtctaagc ctcagtccgg gcaccccacc tcgcctgagc tcaagcttca agagtctgtg
     1381 actaagagaa ttccgaaagg atgtggagcc ctctgactgg ggtgggcggg tgtcctccaa
     1441 ggggcctccg gaagcccaca gagggatgcg ctcctgctca ggcaggtgtc agaagcttgc
     1501 aggggctgtg gccgcaacct gtgattaaag cattcctttc ctgcgtttcc cccttcacca
     1561 ctaatggctg gcctttctgt gtgctgaggt ctttcgacag ttcaaatcat ctggtggcag
     1621 cagactcgcc tttgcccttc tgcggccgag ggcggagatt tatgactttc tctgcttggt
     1681 tggagaagaa gaatctttac tattcagctt cttttctttt tggccagaac tctgaaaaaa
     1741 aaaaaaactc tttttaagac aatatttgta ttctcacagg ctcagctgtc aatcacttga
     1801 gaccttccct gtaaagtggg gcagatttta aatatgggtg tagatactgc ttgcagcctt
     1861 cgcaggaatt ttggttgtgg ttcattgatt cacacagact ctgtgtcagc tgacagggct
     1921 gtgtggggca tcaaaggagg accaggcact gtggagaaga cccattcact ggcatctcac
     1981 ccttccttgt ccagctccat acccagtcct aagacccagt gaaaagccac gtccaaactg
     2041 tgctctgggc tcatcagtgc ccacccacgt accagggaaa ggcacacacc ctacccagtg
     2101 ggcacagagc ggaatgtccc cctaccgcac catttgcgcc cccaatctgg ctgtccaacc
     2161 tagtttgtaa gtaatctaaa tcagtgacta tagccccgcc taagggacac ttcccggagg
     2221 agggagccgc tgaaaaggag ttagtttgag ggtcagtaca caacaggggc agaaagccaa
     2281 gcagatgtgg gggcagggag agtcatcatc tgcttttgtc tgagagaagg agagcttctc
     2341 cgtttgttca actttgtaac aagctgggtt acatgctcca cgcagctaga gaagcctagg
     2401 tgctctgcat tccctgggga actgcaggaa agccttacct gctgactgtt gctctgggga
     2461 aaagcctgag ggtccagagc agctacaagc tacaggccat accttacaac ctgaaaagct
     2521 aaggaccacg gtgaccttcc cggctactgt gtgaaggtgc tgggtggggc ctgctcaaca
     2581 gacagggtcg acagagtgtg tgatacatgc aaacagaatc cttggagtgt gtgatacatg
     2641 caaacagaat cctgggcccc tgcttctccc cctcagtcaa agcaggagtg tcccttccga
     2701 agccaggaca acctgttcac aaggcccctt gtcacatgtc accttccacc tgcctcaagg
     2761 agtgctagtg tccaaatatt tatttttgta ttctcttaag aagtattgat ttcatccttt
     2821 attaaaaaaa gttgctcttt cacaaaaaaa aaaaa
//
