LOCUS       NM_172535               3651 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus IQ motif and ubiquitin domain containing (Iqub), mRNA.
ACCESSION   NM_172535 XM_912624
VERSION     NM_172535.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Zhang,Z., Zhou,H., Deng,X., Zhang,R., Qu,R., Mu,J., Liu,R.,
            Zeng,Y., Chen,B., Wang,L., Sang,Q. and Bao,S.
  TITLE     IQUB deficiency causes male infertility by affecting the activity
            of p-ERK1/2/RSPH3
  JOURNAL   Hum Reprod 38 (1), 168-179 (2023)
   PUBMED   36355624
REFERENCE   3  (bases 1 to 3651)
  AUTHORS   Zhang,X., Xiao,Z., Zhang,J., Xu,C., Liu,S., Cheng,L., Zhou,S.,
            Zhao,S., Zhang,Y., Wu,J., Wang,Y. and Liu,M.
  TITLE     Differential requirements of IQUB for the assembly of radial spoke
            1 and the motility of mouse cilia and flagella
  JOURNAL   Cell Rep 41 (8), 111683 (2022)
   PUBMED   36417862
REFERENCE   4  (bases 1 to 3651)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 3651)
  AUTHORS   West,D.B., Engelhard,E.K., Adkisson,M., Nava,A.J., Kirov,J.V.,
            Cipollone,A., Willis,B., Rapp,J., de Jong,P.J. and Lloyd,K.C.
  TITLE     Transcriptome Analysis of Targeted Mouse Mutations Reveals the
            Topography of Local Changes in Gene Expression
  JOURNAL   PLoS Genet 12 (2), e1005691 (2016)
   PUBMED   26839965
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3651)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 3651)
  AUTHORS   Lai,C.K., Gupta,N., Wen,X., Rangell,L., Chih,B., Peterson,A.S.,
            Bazan,J.F., Li,L. and Scales,S.J.
  TITLE     Functional characterization of putative cilia genes by high-content
            analysis
  JOURNAL   Mol Biol Cell 22 (7), 1104-1119 (2011)
   PUBMED   21289087
REFERENCE   8  (bases 1 to 3651)
  AUTHORS   Shi,Y.Q., Li,Y.C., Hu,X.Q., Liu,T., Liao,S.Y., Guo,J., Huang,L.,
            Hu,Z.Y., Tang,A.Y., Lee,K.F., Yeung,W.S., Han,C.S. and Liu,Y.X.
  TITLE     Male germ cell-specific protein Trs4 binds to multiple proteins
  JOURNAL   Biochem Biophys Res Commun 388 (3), 583-588 (2009)
   PUBMED   19706271
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB572652.1, AK133019.1 and AC155284.7.
            
            On Aug 8, 2009 this sequence version replaced NM_172535.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029931.1, BC137937.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                BB572652.1         2-21
            21-967              AK133019.1         2-948
            968-2533            AK133019.1         950-2515
            2534-3651           AC155284.7         77093-78210
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 11.28 cM"
     gene            1..3651
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /note="IQ motif and ubiquitin domain containing"
                     /db_xref="GeneID:214704"
                     /db_xref="MGI:MGI:3041159"
     exon            1..88
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            89..477
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..2459
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /note="IQ and ubiquitin-like domain-containing protein"
                     /codon_start=1
                     /product="IQ motif and ubiquitin-like domain-containing
                     protein"
                     /protein_id="NP_766123.2"
                     /db_xref="CCDS:CCDS19941.1"
                     /db_xref="GeneID:214704"
                     /db_xref="MGI:MGI:3041159"
                     /translation="MSDPEEERVADSTAHYEEAGKVISIPRPSDEAEGSDVMPEQDDE
                     VQELTTESEENVERHSEFSLSTPKSDDSKPREEVTSLGSASGSQDQDYRLLDYQKGED
                     DELLFLHKIKAIKETLQTSVRESIATVKIVLIPAGQEIIMPFRVDAPFRFLKEHFAHL
                     LHIPHYVLQITHEGIIVGNNESLIQYGIKPQEIVQVEVFSTLPDQYPVRRIEGLSEGS
                     QIITVTIQTSIDRYEEVAVEIIKSDFHKPFLGGFRHKITGLEYHNAGTQTVPRKIPEK
                     DNLFCRDTQTVFQKKKLQQTTNTTSTQMTKIGVYVSNMTDKLLKPGNYFSAAEYHARR
                     LHAVIVIQTSYRRWHAKRYVESLRKQKKLRLEWEEEQELLKIQEKEEWIRMDYYRRHN
                     PKTTEDFELLYNALELWRQEEVEQICHYSSEAERKAALCELLEKETQMIASIGRHRSA
                     ARMERQDAAIQAFLDKCSAPNVWRRGDGKTIEMDTQFTIRARELQSIYKCILLKDLSQ
                     DERLDILLTLKHTVKEHECKLTQEILELIDREVDLMMRGVKPHNLEGLRKRITTLFIH
                     YIKTPLFNPEVAKYLKVPQDPLKFYDTIYFCHSCQNYLPSVEFSVSPTSHRVYRCRHC
                     INLENETQRRESFLKYKCLLQRLYYSEADYGDNSQIAFLMQLQDIKYLTENIWASQSA
                     LSAWDDLNDLVMVRWDKHVEWSPWNCILLTKDESTAHLRLPSIEKGYGHHFVHKIKHK
                     HILAKNYFSQIPTLASLILNDDEVEDIRSKHSTKSPPKIIITRRIQPH"
     exon            478..612
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            613..774
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            775..947
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1103
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1314
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1490
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1661
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1838
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            1839..2087
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2273
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..3651
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2512..2517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /note="hexamer: AATACA"
     polyA_site      2541
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
     regulatory      3051..3056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /note="hexamer: AATAAA"
     polyA_site      3074
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /note="major polyA site"
     regulatory      3627..3632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
                     /note="hexamer: AATAAA"
     polyA_site      3645
                     /gene="Iqub"
                     /gene_synonym="4932408B21Rik; Trs4"
ORIGIN      
        1 acgccagcaa cagaacatct aagcagaaag gagctgcttc cgaggactgg tgaagcctct
       61 tggtctgtga tagctatttc ttgaacagga aaatgtctga tcccgaggaa gaacgtgtcg
      121 ctgactccac cgcccactat gaagaggctg gcaaagtcat cagtattcct cgtccttcag
      181 acgaagcaga agggtcagat gtgatgccag agcaagacga tgaagtgcag gaactcacca
      241 ccgagagcga ggagaacgtg gagaggcata gtgaatttag tctttcaacc cccaaatccg
      301 acgactccaa acccagggaa gaggtcacgt cccttggaag tgcctcagga tctcaggatc
      361 aagattacag acttttggat taccagaaag gagaagatga tgagctgtta tttctgcata
      421 agataaaagc tataaaggag actttgcaga cgtcagtgag agagtctata gcaacagtaa
      481 aaattgtact tattcctgca ggccaggaaa ttataatgcc ttttagagtt gatgctccat
      541 ttagattcct caaggagcac tttgcacact tattacatat cccacattat gtactgcaaa
      601 taacacatga aggcatcatt gttgggaata atgaatctct aatacaatat ggaatcaaac
      661 cacaagaaat tgttcaagtg gaggtgtttt ccacacttcc tgaccaatat ccagtcagaa
      721 gaatagaggg cttgagtgag ggctcccaga tcataacggt caccattcag accagcatcg
      781 atcgctatga ggaggtagca gttgagataa taaaatctga cttccacaag ccatttcttg
      841 gaggatttag acataaaata acaggactag aatatcacaa cgctggaaca caaactgtac
      901 ctagaaagat tcctgaaaaa gacaatctgt tctgtaggga cacccagaca gtttttcaga
      961 aaaaaaagct acaacaaact acaaatacca cctccactca gatgactaaa attggtgtgt
     1021 atgtatcaaa catgaccgat aaactgctca agccaggaaa ttacttttca gcagcagagt
     1081 accacgctcg acggttgcat gcggttatag tgatacagac ttcctacagg cggtggcacg
     1141 ctaagcgcta tgtagaaagc ttaagaaaac aaaagaaatt gaggttggag tgggaagaag
     1201 agcaagaact cctgaagatt caagagaagg aagaatggat acgaatggac tattaccgga
     1261 ggcacaaccc taaaacaact gaagattttg agctccttta caatgctctg gaactctggc
     1321 ggcaagaaga agttgaacaa atttgccatt attcaagtga agctgaaagg aaggctgctc
     1381 tgtgtgagct attggaaaaa gagacacaga tgattgcttc catcgggaga catagatccg
     1441 ctgctcgtat ggagaggcag gatgcagcta tccaagcttt tctggataag tgttcagctc
     1501 caaatgtctg gagaagaggt gatggcaaaa ccattgaaat ggatacacag ttcacgataa
     1561 gagccagaga gctgcaaagc atctacaagt gtatcctgct gaaagacctc tctcaagacg
     1621 agaggctgga tatactcctg acgcttaaac acactgtgaa ggaacatgag tgtaaactga
     1681 ctcaagaaat cctagaactg attgacagag aagttgacct tatgatgaga ggagtcaaac
     1741 ctcataatct cgaaggactc agaaagagaa tcacaacgct ctttattcac tacatcaaaa
     1801 ctcctctgtt taatcctgaa gttgcaaagt accttaaggt ccctcaagac ccactgaagt
     1861 tttatgatac gatttacttt tgccacagtt gccaaaatta tttgccttct gtggagtttt
     1921 ctgtctcacc cacctcccac cgtgtgtacc gctgccgcca ctgcataaac ctggagaatg
     1981 agactcagag gcgggaatcg tttttgaagt acaagtgttt acttcagcgg ctctactact
     2041 ctgaagctga ttatggggat aactcccaga ttgcttttct gatgcagctt caggatatta
     2101 aatacttgac agagaacatc tgggcatccc agtcggccct cagtgcctgg gatgatctca
     2161 atgacctggt catggtcagg tgggataaac acgtggagtg gtctccctgg aactgcattc
     2221 ttctcaccaa ggatgaaagc acagctcacc tcaggctacc gagtattgaa aaggggtatg
     2281 gacaccattt tgttcacaag atcaagcaca aacatatcct ggctaaaaac tatttttcac
     2341 agatcccaac gttggcgtca cttatactta atgatgatga agtagaagat atcaggagta
     2401 aacactccac aaagagtcca cccaaaatca taataacccg taggattcaa cctcattaga
     2461 ttcaagaata tcaatttgaa gatgagaggt tttgttctct acgatgagag taatacagag
     2521 gtcatgtgac atggaaaacc acctatatat ttctatttgt attatttgct ttactgagta
     2581 ctttgttata tgaatagaaa aaatctgtat acacaaaaaa ccacccttgt taggtggtta
     2641 gtaatctata aatttgatac taacattaat agttaaagag aacattttat ttttatctat
     2701 tttcatgtta atttaagaaa atgctttata gtcaaaatac atcagtgaaa caaaaccttc
     2761 agtgaaagta atatatttat ttaacatata ctttatgagc atatttactt tctgtacata
     2821 agcctgtatt ttgacataag tatttgaata tttgactttt attgtctact tttacctttt
     2881 aactgcataa tacaagaaaa gaaactatag caaacaaagc gaaacatctg cattgcatgc
     2941 catgaaacaa ggcaaacagt agaaacgtta tgttaaaagc ttcctacagg gtagtgttga
     3001 gattctgtaa gaagcataaa cttgatcaaa tcaactgtcc actgcttctc aataaactaa
     3061 tcaataacca ctgactccat atctgtctga aatatgtgat gatgatgtat ccaaacgtgg
     3121 cacaccccaa agagcatggt tcaagaagag cttatttaca aaaaaggccc tactctacga
     3181 catacgtgac acagggaaac agaaggaatc cagggtcatg tttcaggtat acaaaatctg
     3241 atggagaagc cagagaaccc agagagaatg gaaaggagat aagtagatca ctgcgaggac
     3301 tggtaaccag tctgcaaaag aacaaccaaa ctaagatgac ctcaagaact agataacaaa
     3361 cccccagtct cacaccctcc tttgctccag ttgccttctc catttggaaa cagaggaaaa
     3421 gcatacaagt ttccatggtt ttagagggaa gagaagggga gttaatggtt gaacagttat
     3481 ggagggctaa atagaaagca ttgggaaaga aaaacaacaa agtggggata taattcaaat
     3541 tagtaaaatc tcatgttata gaactataga aacacatttt actctaattc tatgtaaatt
     3601 atgttgttct ttcaagtcta gtgattaata aattcattat aaatatttgc t
//
