LOCUS       NM_199056               4429 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ippk),
            transcript variant 1, mRNA.
ACCESSION   NM_199056 XM_283126
VERSION     NM_199056.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Franco-Echevarria,E., Sanz-Aparicio,J., Troffer-Charlier,N.,
            Poterszman,A. and Gonzalez,B.
  TITLE     Crystallization and Preliminary X-Ray Diffraction Analysis of a
            Mammal Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
  JOURNAL   Protein J 36 (4), 240-248 (2017)
   PUBMED   28429156
  REMARK    GeneRIF: Crystallization and preliminary x-ray diffraction analysis
            of a mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase has been
            reported.
REFERENCE   3  (bases 1 to 4429)
  AUTHORS   Franco-Echevarria,E., Sanz-Aparicio,J., Brearley,C.A.,
            Gonzalez-Rubio,J.M. and Gonzalez,B.
  TITLE     The crystal structure of mammalian inositol
            1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding
            site and key features for protein function
  JOURNAL   J Biol Chem 292 (25), 10534-10548 (2017)
   PUBMED   28450399
  REMARK    GeneRIF: findings have uncovered distinct features of a mammalian
            IP5 2-K and set the stage for investigations into protein-protein
            or protein-RNA interactions important for IP5 2-K function and
            activity
REFERENCE   4  (bases 1 to 4429)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 4429)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   6  (bases 1 to 4429)
  AUTHORS   Verbsky,J., Lavine,K. and Majerus,P.W.
  TITLE     Disruption of the mouse inositol 1,3,4,5,6-pentakisphosphate
            2-kinase gene, associated lethality, and tissue distribution of
            2-kinase expression
  JOURNAL   Proc Natl Acad Sci U S A 102 (24), 8448-8453 (2005)
   PUBMED   15939868
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC123954.4.
            
            On Dec 13, 2019 this sequence version replaced NM_199056.3.
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the shorter isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK167467.1, AK160092.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-225               AC123954.4         133439-133663       c
            226-273             AC123954.4         123989-124036       c
            274-369             AC123954.4         122440-122535       c
            370-436             AC123954.4         121010-121076       c
            437-558             AC123954.4         119314-119435       c
            559-648             AC123954.4         118105-118194       c
            649-707             AC123954.4         112894-112952       c
            708-780             AC123954.4         111411-111483       c
            781-1054            AC123954.4         108329-108602       c
            1055-1205           AC123954.4         105518-105668       c
            1206-1308           AC123954.4         104822-104924       c
            1309-1388           AC123954.4         96213-96292         c
            1389-4429           AC123954.4         90331-93371         c
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 25.25 cM"
     gene            1..4429
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /note="inositol 1,3,4,5,6-pentakisphosphate 2-kinase"
                     /db_xref="GeneID:75678"
                     /db_xref="MGI:MGI:1922928"
     exon            1..225
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..1614
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /EC_number="2.7.1.158"
                     /note="isoform a is encoded by transcript variant 1;
                     inositol-pentakisphosphate 2-kinase; insP5 2-kinase;
                     ins(1,3,4,5,6)P5 2-kinase"
                     /codon_start=1
                     /product="inositol-pentakisphosphate 2-kinase isoform a"
                     /protein_id="NP_951011.1"
                     /db_xref="CCDS:CCDS26500.1"
                     /db_xref="GeneID:75678"
                     /db_xref="MGI:MGI:1922928"
                     /translation="MEEGKMDENEWSYHGEGNKSLVVAHAQRCVVLRFLKFPPNKKKT
                     SEEILQHLQNIVDFGKNVMKDFLGENYVHCGEVVQLPLEFVKQLCLKIQCERPESRCD
                     KDLDTFSGYAMCLPNLTRLQTFHFAEHRPILCVEIKPKCGFIPFSNDVTHEMKHKVCR
                     YCMHQHLKVATGKWKKISKYCPLDLYSGNKQRMHFALRSLLQETQNNLRIFKNGELIY
                     GCGDARSPVADLKELAHHLKPFFFPSNGLASGPHCTKAVIRELVHVITRVLLSSSEKA
                     RAGALRLGLQGPRVCEASPFSRSLHNQGKNTSEHSGLPKGCLLYKTLQVQMLDQLDIE
                     GLYPLYKRVEQYLEEFPEERKTLQIDGPYDEVFYQKLLDLSTEDDGTVAFALTKVQQY
                     RVAMTAKDCSIMIALSPCLQGTSSDQRPVIPSSRSRLAFSVSVLDLDLKPYESIPHQY
                     KLDSKIVNYYSKTVHAKDDTVRSTRFKEHEDCTLVLHKV"
     exon            226..273
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            274..369
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            370..436
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            437..558
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            559..648
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            649..707
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            708..780
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            781..1054
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1205
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1308
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1388
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..4429
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2820..2825
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /note="hexamer: ATTATA"
     polyA_site      2843
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /note="major polyA site"
     polyA_site      2917
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
     regulatory      4395..4400
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /note="hexamer: ATTAAA"
     regulatory      4405..4410
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
                     /note="hexamer: ATTAAA"
     polyA_site      4416
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
     polyA_site      4429
                     /gene="Ippk"
                     /gene_synonym="1810043M15Rik; InsP6"
ORIGIN      
        1 gcctgcaagt cgccgccgct gagagcgcag gcggcagctg acaggcgctt ttcttcagac
       61 ggctgcggcg ggagccctgc gaggcgggcg gcctccagct ctcgtcccca cgtcccggcc
      121 cgcgaagttc cagggcgccg gggcatggaa gaggggaaaa tggacgagaa tgaatggagt
      181 taccatggcg aaggcaacaa gagccttgtg gtggcccacg cgcagcgctg tgtagttctg
      241 cgttttctga agtttcctcc aaataagaag aagacctcag aagagatact tcagcatctg
      301 cagaacatag tagactttgg caaaaatgtc atgaaagact ttttgggaga gaactatgtt
      361 cattgtgggg aagttgttca gctgccttta gagtttgtga aacaactctg tttaaagata
      421 cagtgtgaaa gaccagagtc ccgttgtgac aaggacctgg acacattcag tggttatgct
      481 atgtgccttc ccaatttaac cagacttcag accttccact ttgcagagca ccggccgatt
      541 ctgtgtgtag agattaagcc aaaatgtggg tttattcctt tctcaaatga tgttacccac
      601 gagatgaagc acaaggtgtg ccgatactgc atgcaccagc acctcaaggt agcaactgga
      661 aagtggaaga aaatcagtaa atactgtccc ctcgacctct actcaggaaa taagcagcgc
      721 atgcactttg ccctcagaag tctgctgcag gagacacaga ataatcttag gatatttaag
      781 aatggagagc tgatttatgg ttgtggtgat gcccggagcc ctgtggctga cttgaaggag
      841 cttgcacacc acctgaagcc cttcttcttc ccttccaacg gcttggccag tgggcctcac
      901 tgcacgaagg ctgtgatccg tgagctggtg catgttatca ccagggtgct gctgagcagc
      961 tcagaaaaag cccgagctgg tgccctgagg cttgggctcc agggcccacg agtctgtgaa
     1021 gccagtcctt tcagcaggag cctacacaac caaggaaaaa acacctcaga gcactcgggg
     1081 ttaccgaagg gctgtcttct gtacaaaacc ctccaggtgc agatgttgga ccagctggac
     1141 atcgaaggcc tctaccctct gtacaaacgg gttgagcaat acctggagga gtttcctgag
     1201 gagaggaaaa cattacagat agatgggcct tatgatgaag tattttacca gaaactgctt
     1261 gatctgtcca ctgaggatga tgggacagtg gcctttgcat taacaaaggt gcagcaatac
     1321 agagtagcca tgactgccaa ggactgttcc atcatgatcg cactttcccc ttgtctacag
     1381 ggcaccagct ctgaccagag gcctgttatt ccttcgtcaa gatcccggct ggccttctct
     1441 gtgtctgtat tggacctcga cctcaagccc tatgagagca ttcctcatca gtataaacta
     1501 gacagcaaga tagtcaacta ttactcaaag actgtgcatg ccaaagatga cactgtgagg
     1561 tcgactcggt tcaaggaaca tgaagattgc acattagttc tccataaggt ctaacttttc
     1621 ccctgcggtg tctttgaaac ttgaatgtga aagttgaatg gcagagctat tttcggttgt
     1681 gttgggtgac ttggctgtga atgtctttgc ttttaacccg ggtctgctga ggttagactg
     1741 cctctccaag acaaggagtt gaaagcccta tagtcttcta ggacgaggaa tgtaggacgt
     1801 gaggctgtgt cccagcaagc catccgtttc cttagagtgc aaatgtccct tgagccccag
     1861 gcaaacatcc tgggtggttc ttccaagcag agctcacgtc cagttctcat acgaaagcct
     1921 tattattagg acccacgctt tggatctcct accaccagac cattgacaca gaaacttgaa
     1981 ttgttacgag tagcttacgg tttggatttt cacgaaaata tggatactac tggaactttc
     2041 ccagctgtta actggtcact tgttcaatgc aagtcacata tcaacacagg ctttgggtgg
     2101 tcccctgccg cagagcggtt agggcttagt atggacattg gttctgaggg gtttagactt
     2161 aggtccctgt ggaggtaccg aagcccatag ctttgtactg gggcagcagc agggaacaca
     2221 ggactcaggt caggatggac accactcaca tgagcatttt tttttttttt aaattcttta
     2281 tgattttaac tctagggttg ctgaggcgtt ttttcctacc tgacattgct gctgtagagg
     2341 ctggaggcag agctgtagtt cagagctgct aaggtctgct gcgtgggtgt cctgacagcc
     2401 cagagttttc ttggcattct ggtgtcttgg atggttgagg atcaaggttt cccttcctcc
     2461 taagccacat tcatccctgg gttagctctg aggcatcctg ggtatttggg tctagggcct
     2521 tactgttttc atcagggcta gatagaaggc tcctagctgg cactgagaat tctacggggt
     2581 caccagaagt gtgcctggct gctatgaaat cattgggagt ttttgtcttc atttgttttt
     2641 gaaatcctat ggtaggctgt acagattgat tatttatatc atccttttga gagactaatg
     2701 aaggcttatt gtaatatagt agtaaaacca tggaactgta tgaaaaatac tacatgaaaa
     2761 atgaagaaaa tattttgctg attgtaaact tttgtgggaa attttgtgat aatttgagaa
     2821 ttatacttgt ttgaatcaag caacatccta tagagtttgt taaattctgt cctaatattc
     2881 tagctcttga acactcaggt aaagacagac acttgtacat ttggatcgtt cgattttttt
     2941 taaactggtg tatgcaaaca agcatatgtg cctatatcct tcacctgagt cacatgcttg
     3001 aaggtcatgg ttttgttttg actcagggat gcctgctgtg gaggtggact aggggtatat
     3061 gtttgtgtgt gtctttggtt gtctgaggcc atagtaatga tctaagatat ggctgggtcc
     3121 caggctctca gagcagtttc acagaaacct gtatttctag aatgctggtc tgttttagag
     3181 aattataatt ttgcaagagg ccctgtcttg gatagatagg tagatggaaa caaacagtgt
     3241 ctcctggagt aaagaacaca ggtggagcac cccgccaaca tgtacagggc tctcctccaa
     3301 ccatactcct aattctgctt ggttcaggat acccacacca acccctcatc ttggcagcac
     3361 atcaggtaac tgaggcactc accaaacaat atagctctgt gtctgagata atgcggcctt
     3421 agcctgggta gttcttatgt ggtacggggg gattggactt aggggccttg cacattttag
     3481 gcaattgttc tactaagatg gaggtataat ctagcctttg ttttctgaga cagtggctca
     3541 ctatatagcc aaggctgctt taaactttgg ttgctcttgt gtgctggaat tatagttatg
     3601 taccaccctc aggctgtggg tgtgtcttca agtttccaag taaaggcaac agtgctactt
     3661 ctgtaggaga ccgagaggag tatcggggct tgctgtgaga tttctgctgg gcagaccaga
     3721 tggatagctt tgggtttttc tgtgtcccat gctgtcttgg gccaacctga tgtgaggttg
     3781 accgctgaac acagctcttt cacactcatt tgagaatgga caaccattct tggaacattt
     3841 tctcaaatac taaacttaac tgtacaaagc actatactat taacatatct taatagcttt
     3901 attttttttt atgccatggg agttcatatg taatatacca ctaatgcaca caaactcatg
     3961 tctgaaggat atggtttcta attggaaaag agaaatgctc atagtcaaca tattttgaat
     4021 gttcctcagc ccagcaatgt tcctgtgtgt ttgccagcgg gagactagtt gttatctcca
     4081 ctgaacaatc ttatcaggct gtctagagca gcttcaatac caagtacacc tagcaggctt
     4141 cggaaactga gaggagcagt ttctatgact ctgttcttgt ccagtcctag ggctgtgaag
     4201 aaaacaaacc tggtaagaaa catcagaatg tcttaagaca gcacttcccc ccaatagcca
     4261 cacagtggaa agcacagact gaagaccaag gggcaatgtc acctgtaacc caaggcagag
     4321 aaaatgccgc cttccacaca tcctgcaaag gcttcaggaa agtggcttct tcttgtgtgt
     4381 ttgggtggtt actgattaaa gtatattaaa agctgatcta caaagtgca
//
