LOCUS       NM_001025382            5371 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus inhibitory synaptic factor family member 2B (Insyn2b),
            mRNA.
ACCESSION   NM_001025382 XM_894881 XM_906417
VERSION     NM_001025382.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Feenstra,J.M., Kanaya,K., Pira,C.U., Hoffman,S.E., Eppey,R.J. and
            Oberg,K.C.
  TITLE     Detection of genes regulated by Lmx1b during limb dorsalization
  JOURNAL   Dev Growth Differ 54 (4), 451-462 (2012)
   PUBMED   22417325
REFERENCE   3  (bases 1 to 5371)
  AUTHORS   Chen,H., Palmer,J.S., Thiagarajan,R.D., Dinger,M.E., Lesieur,E.,
            Chiu,H., Schulz,A., Spiller,C., Grimmond,S.M., Little,M.H.,
            Koopman,P. and Wilhelm,D.
  TITLE     Identification of novel markers of mouse fetal ovary development
  JOURNAL   PLoS One 7 (7), e41683 (2012)
   PUBMED   22844512
REFERENCE   4  (bases 1 to 5371)
  AUTHORS   Bouma,G.J., Hudson,Q.J., Washburn,L.L. and Eicher,E.M.
  TITLE     New candidate genes identified for controlling mouse gonadal sex
            determination and the early stages of granulosa and Sertoli cell
            differentiation
  JOURNAL   Biol Reprod 82 (2), 380-389 (2010)
   PUBMED   19864314
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL671971.8 and AL669849.19.
            
            [WARNING] On May 10, 2024 this sequence was replaced by
            NM_001025382.3.
            
            On Apr 8, 2010 this sequence version replaced NM_001025382.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.166769.1,
                                           SRR1660821.126538.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               AL671971.8         131000-131123
            125-2374            AL669849.19        15977-18226
            2375-2449           AL669849.19        19199-19273
            2450-5371           AL669849.19        34640-37561
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 19.72 cM"
     gene            1..5371
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /note="inhibitory synaptic factor family member 2B"
                     /db_xref="GeneID:574403"
                     /db_xref="MGI:MGI:3643491"
     exon            1..124
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /inference="alignment:Splign:2.1.0"
     exon            125..2374
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /inference="alignment:Splign:2.1.0"
     CDS             1029..2636
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /note="protein FAM196B; family with sequence similarity
                     196, member B"
                     /codon_start=1
                     /product="INSYN2B protein"
                     /protein_id="NP_001020553.1"
                     /db_xref="CCDS:CCDS36127.1"
                     /db_xref="GeneID:574403"
                     /db_xref="MGI:MGI:3643491"
                     /translation="MAQQSMKVRPVLLKRSSLESVELVKQPHHRRSKSQQVRFKEDGN
                     IKNPTGVTEVNTQTPEDPGVMGKAQASRHHHPTTYSLSFPRSHKAGGFRSISIQTSPS
                     LRKHFPVFKRKKLTTSKSLVEMPTASPSAIQVNGNLSEQDIVSSDLAFLRLAQHLEDG
                     PRRLKIPHPFLPRMPKVQSNGPVSFCLESGTWMSSEKATAAIQVPDDICHSPTWEARE
                     SALSPESSAEESNSIPALISMCPGDGQRVMTSELERMPPCSNTNSSASNMPGTEKLTP
                     ELLLPKDNPDDKDLGLPSSQSKKMCVPSPPRTHSSPEPGSRSQPVHLGRSSDCPASGD
                     NHQDLESLRSSSASKSVPVCWEHVTKLPSQSDTPELQTGVGSEQLPASIPRQENRAQS
                     SREIGGSNHSHLAQGELCDLQGRLQSVEESLHSNQEKIKVLLNVIQDLEKAHALTEGR
                     NFYRTGQDLNNCSTCQNTACIIYSVEYDFRQQEGRFHEVLQSLEEAEPTEEAPSPPKS
                     PAEAPVPEKQDLRRKSKKVKKKCFWWI"
     exon            2375..2449
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..5371
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5349..5354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /note="hexamer: AATAAA"
     polyA_site      5371
                     /gene="Insyn2b"
                     /gene_synonym="EG574403; Fam196b; Gm6041"
                     /note="major polyA site"
ORIGIN      
        1 tgaaaattac aatataaagg gcttattttt tccttcgttt ggagttatct tgcttctggt
       61 caaaggctgg ctcgttgtac aaggaagggg cggcatcttc tgcaggtaat tccactgctt
      121 ggaggcactt tctcactgct cttaaaggct gtctggtctc caaagcttgc cccaccaatg
      181 cagtaactgt ggttctcagc tgaacacctc caaataaagg ctgtgggggt ggctcactcc
      241 tcatgtgtgg atttcagcca agcctgcaac acagagcctt gccctctgag tctcctccat
      301 gcctcgattc tgccctagtc atcctcttct gggttttatg aggctattta aggaattacg
      361 tttccctcac tgaaacacag aaaagcaaca tggacgagaa caatggcccc aacggagaaa
      421 tttctgattg acatgagtga agaagttccc ttgggaactt catgtgccat caaagtagga
      481 aagccccagg tcatctctta ctcttcgcat ctacgtgcca cggcaagatg tgtttaaggt
      541 tcccatagcc ttgcactgca atttgatgct ctggggaaag cagccatatg gtttagacat
      601 agcaggtttc tcttggagtt cacacattcc cagcgtggca ctatccacca aatggtacat
      661 ttattgtctt tttgcgcaag acagggaact tctaggatca gaactgctcc ttggaatcca
      721 ctgacagatg aacctatcgg ttctcttggt aggtcagatg tttcataatt agcctaactc
      781 actgatgatc tgttcagcgc ttatgattat tgcagtggca caatatttca actgcgggtg
      841 aagcactcca gacagctcct aagcagacca gtcaagctgg gaatggccgg cagggcttcc
      901 tgtattaaga ggagagaacc actacatcgt gcttggccat cctccttgtc tccatcatta
      961 tacactgctc taaagaccta agggagatgt gcccaggaca agcgtgcggt tcttctcagt
     1021 gaggctccat ggcccagcaa agtatgaaag tgaggcctgt gctcctaaag cgaagcagcc
     1081 ttgagtctgt ggagcttgtg aaacaaccgc accaccgcag gagcaaatcg cagcaagtgc
     1141 gattcaagga agatggcaac attaagaatc caactggcgt gaccgaggtc aacacccaaa
     1201 cccccgaaga cccaggtgta atggggaagg cacaggcctc caggcaccat catcctacca
     1261 cctactcgct gtctttcccc aggtcccaca aggcaggagg attccgcagc atctccatcc
     1321 agacctcccc cagtctcagg aagcatttcc ccgttttcaa aaggaagaaa ctcacgacta
     1381 gcaagtccct ggtggaaatg ccaacagcat ccccaagtgc catccaggtc aacggcaacc
     1441 tatctgaaca ggatattgtg tcctctgacc ttgccttcct gaggctggct cagcatcttg
     1501 aggatgggcc tcgcaggctc aaaatccccc acccatttct ccctaggatg cccaaggtgc
     1561 aaagcaatgg gcctgtcagc ttctgcttag aatctgggac ttggatgtcc tcagagaaag
     1621 ccacagctgc tattcaggtt ccagatgata tttgtcacag tccaacctgg gaagctagag
     1681 agtcggctct cagcccagaa agctcagctg aagagagcaa ctctatacct gctttgatct
     1741 ccatgtgtcc aggggatggg caaagagtga tgacgtcaga gctggaaaga atgcccccct
     1801 gctcaaatac caatagcagt gccagtaata tgccgggcac agaaaaactc acacccgaat
     1861 tgcttttgcc caaagacaac cctgatgaca aagaccttgg ccttccatca tctcagtcaa
     1921 agaagatgtg tgttccctca ccaccacgga ctcacagttc gcctgagcca ggctcccgta
     1981 gccagccagt ccacctagga agatcttctg attgcccagc ctcaggtgac aatcaccagg
     2041 acctggagtc actcagaagt agcagtgcat ccaagtctgt ccctgtgtgc tgggaacatg
     2101 tgacaaagct ccccagccag tcagacaccc cagagcttca gactggtgtt ggaagtgagc
     2161 aactccctgc ctcgattcca aggcaggaga acagagctca gagcagcagg gagataggtg
     2221 ggagtaatca cagtcacctg gcgcagggag agctctgtga cctccaaggc cggctgcaat
     2281 cagtagagga atcgctgcac tcaaaccaag agaaaattaa agtcctattg aatgtgattc
     2341 aggatctgga gaaagctcac gctctcactg aagggcggaa cttctatcgg actggccaag
     2401 atctcaacaa ctgcagcacc tgccagaaca cagcatgcat catatacagt gtagagtatg
     2461 atttccggca gcaggaaggc aggttccatg aggttctcca gagtctggag gaggcagaac
     2521 caactgagga agcaccttcg ccacccaaat ccccagcaga agccccggtc cccgagaaac
     2581 aggacttacg gcggaagagc aagaaggtga agaagaaatg tttctggtgg atatgagaag
     2641 attggggctt cctgcattcc ttcctgagat gggggatccc ctcaagacat ccccgatcct
     2701 ctgcaaaagc gcaggccgag atccccttgc cctgtgagac aggagtgcct cagagccggt
     2761 acacccctgg acacctgctc accaacctta cctgagtgtg tgccgcagct tgcagaggat
     2821 tttgtggaca cttgcgtggc cacttgttac cgagctgcct gtccctcaca gccaggcttc
     2881 gtcctcatct gctaagctca gattcccacc atatgctaat gctcactgca ctcccaccac
     2941 ttccctggca gcaagcattt ccttcctacc ctgagatcct cgaccccaaa ggtacagaaa
     3001 taagcctgtg ttgtgcagtg caggaagagg agggttgtga gcaagtatcc tagaaaacat
     3061 cacacaataa gctggcaggg ttctaggatt taccgtacca aaagcacaac agaaatgagc
     3121 agagcctaac aatgccaggt ccccctgatt gtattaagtt gttctctttt tcaactagct
     3181 gttagatttc aaatccagca gctgtgcagc tgtcttcttg taaagtatgg ggtgtggcag
     3241 agtaaggttg cccatgggta ttagaaacct gttgtgtgat ctatgttact ggaactcagg
     3301 tgactttcat agctgtagtc tttctggggc tgcttaatta tcatatgtct ctgtataccc
     3361 agtggggggg ggggtaattg agaggggctt tatcgttagg gtcatttacc tgttagcgtt
     3421 catttacctg taacattaag ggtcttaaga ctcagcaact tgtatgtcgg tataccttat
     3481 caagtgacgt taacaaggct tacctacaat atagcatttt ttcctcatcc atgcatcttg
     3541 tgacaaccat gtagataggc ccaaacatgc tactctacag ttacccttcc tggtgaccag
     3601 gtatcggtca cctgatctag gaagaaacac gaccgagaat gaggatggaa gcgccttctt
     3661 ccacaaagtt tgagcttctt aagatctagc tcgttgtgtt gacaagacct ccactcatgg
     3721 atgtccggtc aagcttgcat gccaagcaaa ctcccaaagc agtgtgatta agtaattgct
     3781 acctactggg gctgaagagg aggcaaggct tggatacctc tgcattcagt tctgtgttct
     3841 gccagcctgt gattggactg acactgccag aaatgtgagc cacacttaag caaagaaagc
     3901 cttttataac cgcccccttg ccaaagaccc ggtgtctgag tgagtctctg ctgtgtacgt
     3961 ggggtgtgcg taagcccact gccaattgag gctaattgag caaaccacct ccaacaattt
     4021 cacctccctt gtcacagcgc tgcggtgcaa aagctatttc agggagagtg aggaaaattt
     4081 ggcaaggaat ctgggcttgc actatttaag tcttgggctg tcatttgaga tgttctctct
     4141 ctgtttccag tgtaatgttt aaactcacaa agtgagacca gaatatagaa tgtcaagtta
     4201 gaattaagat aaatatactt gccatgctta agaaaccaca tttcaattag atatgttacc
     4261 actggccaaa agccacagat ctttctttct gtatccacac actcaactct cagttggccc
     4321 cagcagtgga aaaaaacact acagaggata atccaataaa gcaaactcat ttgggatctc
     4381 ttatataatt ttatcttgca gtggggcttc catcttaatt tgttctgctt acactaattt
     4441 caaatgaatt tatattccag aatatcagga tgggatgaaa atattcttgc acttagatct
     4501 cggagactcg gggaaataga atagcctttt tcactaacct ggagaagatg gcataattgt
     4561 gtcctttctt gactgggcag aagaatgact atgcctcctg gtttctaagc tctgtcaacc
     4621 tcctactagt gggggagcac agctgtgaga gctaactgca gcctttgtac tgggtagcct
     4681 ggagaaatga ggagatatct ggggtagctc ttatctttac acgtacatgg caagttaggg
     4741 gatcttccat aaactctgaa tgagattaaa ttaggactga aaactgttac cattccctga
     4801 aagaaaaatc aacccaggtc acgtgatttt ataccaaagc acaacaccac atcgcactgc
     4861 ttaggccaca ctaatctata cctggagaga aggttccttc tggtaattga atcctgagca
     4921 tcagtattgg ggggaaagga ttgataaaca ccagccttct gaacagaaag ggtcatcact
     4981 acattaaata aaaaacatac tcatatatac caaaactcta tagtctgtta ctcgtgttga
     5041 gtagcaatgc ttctttgtaa aatagtatgt aatatgatat tctacagaaa tgttgtactc
     5101 gaggttatat ggttttatgg aaagagattc atttagcaaa aattactttt atgaaatgga
     5161 aggagtaagg actgatgggt ctgtttaaat tgcaggggtt tattttatct gctccctcta
     5221 tagttaaaac actcagtatc cctggaactt aatgccagct catctgtaga gcgccttctt
     5281 gaagacaatg tatcactgta aggttttgta aatttctaaa ctgttttaaa tgggatatta
     5341 tcttttgcaa taaacttaag tatttttcct a
//
