LOCUS       NM_001143802            4637 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus inhibitory synaptic factor 2A (Insyn2a), mRNA.
ACCESSION   NM_001143802 XM_001480332 XM_894743
VERSION     NM_001143802.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Uezu,A., Kanak,D.J., Bradshaw,T.W., Soderblom,E.J., Catavero,C.M.,
            Burette,A.C., Weinberg,R.J. and Soderling,S.H.
  TITLE     Identification of an elaborate complex mediating postsynaptic
            inhibition
  JOURNAL   Science 353 (6304), 1123-1129 (2016)
   PUBMED   27609886
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127574.3 and AC100208.16.
            
            On or before Jan 8, 2009 this sequence version replaced
            XM_001480332.1, XM_894743.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164131,
                              SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-719               AC127574.3         98127-98845         c
            720-1881            AC127574.3         78058-79219         c
            1882-1953           AC127574.3         59561-59632         c
            1954-4637           AC100208.16        271289-273972       c
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 80.86 cM"
     gene            1..4637
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /note="inhibitory synaptic factor 2A"
                     /db_xref="GeneID:627214"
                     /db_xref="MGI:MGI:3605068"
     exon            1..719
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /inference="alignment:Splign:2.1.0"
     exon            720..1881
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /inference="alignment:Splign:2.1.0"
     CDS             725..2137
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /note="protein FAM196A; family with sequence similarity
                     196, member A"
                     /codon_start=1
                     /product="inhibitory synaptic factor 2A"
                     /protein_id="NP_001137274.1"
                     /db_xref="CCDS:CCDS52419.1"
                     /db_xref="GeneID:627214"
                     /db_xref="MGI:MGI:3605068"
                     /translation="MVSKDSGRCILTTPEREVEPAACLALEMRYALDPNRQIKKRNKA
                     LQVRFKDICEAQNEQRDTQLSSGPLGEKREAKAVSCRVAYRKYMTVPARRSIPNVTKS
                     TGVQTSPDLRKCYQTFPLDRKKGSLKGLPAADAFKSQNNGFLADSKEKSEAGPMEEPR
                     PCSAGRIHKTTALVFHSNEHVNALGQPSGVNCAELCKSPDVLGYPEAVLQNSRPPSEE
                     PIHQLQGRAKLDRGTLDSEEPAPLTHGRVFKTEVATVYPPAISTRAPEPGLSNSAAAS
                     QWSLCPADEEQRRAAHLNGLQASTGSAVACSTPVQYLSPECSEQPLQNQPSPTAGIGD
                     EEHQQIVPHTEVVDLKAQLQVMENLISSSQETIKVLLGVIQELEKGEAHREGLSYRTG
                     QDTANCDTCRNSACIIYSVELDFKQQEDKLQPVLRKLHPVEETQVIPSPYPQETHSST
                     PKQKSKTESKKHGRWKLWFL"
     exon            1882..1953
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..4637
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2371..2376
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /note="hexamer: AATAAA"
     polyA_site      2391
                     /gene="Insyn2a"
                     /gene_synonym="B830028B13Rik; Fam196a; InSyn2"
                     /note="major polyA site"
ORIGIN      
        1 ccttctcctc ctcctcctct tgcccgcgat ccgtctctgg ctggaggggc tccttcacgc
       61 gcccggcccc cgccccacgc acgcggcctg gcccgccccc gccccacccc cgccccacgg
      121 gccccggggc tccgcccgcc tcggtggcag gcgagcgcac aggcgactgg cgcgcagctc
      181 ccggtgccct ctcttcagtc ctctggccgc tcggctgcat agagtagccg ggacctggtg
      241 cctcggccag gactcccctc ccgctccctc cccgtctccg ccccctcctc ccggccccgg
      301 ctcccggatc tcgccgaaga caattgcagt aaatgaggac atctgaggag cacggagccg
      361 ggtggctgtg gctggctccg ccgcgccgcg atcgggcgag cgcgcgggcg tccccggagc
      421 cggccgaacc actgcctccc cgtgcgcgtc gctgagcagg agccgaaggg actcgagctg
      481 cggcgcgggg gccggccggg ccgggcgggc cgggcccgtg gcagtggagg gggcgggcgg
      541 cgggcacgca gctggccaag tctccggagc cgagcctcct tcagcgcgag cccggagatc
      601 cttgccgtgg atgctctgcc ccgagtgctc cgtgccgcgc caggcttgcc agggtccgcg
      661 gcaagacatg cccgagccag gcgctgcacc gacctccact tatcagtaag cagccaaagg
      721 caccatggtc agcaaggact ctggcagatg catactcacg acaccagagc gggaagtgga
      781 gcccgccgcc tgcctggccc tggagatgag atatgccctc gaccctaacc ggcagatcaa
      841 aaagcgaaac aaagctctgc aggtgcgttt taaggatatc tgtgaggccc agaatgagca
      901 gagagacaca cagctgtctt cgggacctct gggggagaag cgagaggcca aggctgtgtc
      961 ctgcagggtg gcctatcgta agtatatgac agtgcctgca cgccgatcca ttcccaatgt
     1021 caccaagagc accggtgtgc agacctcacc agatcttcgc aagtgttacc agacattccc
     1081 gctggaccgc aagaaaggca gtctcaaagg cctgccggct gcagatgcct tcaaaagcca
     1141 gaacaatggg tttctagcag attcaaaaga gaagagcgag gcgggaccca tggaggagcc
     1201 tcggccttgc agtgcaggaa ggattcacaa gaccaccgca ttggttttcc attccaatga
     1261 gcacgtgaac gcactgggac agccttctgg ggtcaactgt gcagagctct gtaagtctcc
     1321 agatgtgctt ggctacccag aggctgtttt gcaaaactct cggcctccct ctgaggagcc
     1381 catccaccag ctgcagggga gagctaagct tgacaggggc accctggact cagaggagcc
     1441 ggctccactc acccacggaa gggtgtttaa aaccgaggtc gccactgtgt atccgcctgc
     1501 catcagtacc agggcccctg agcctggctt gtcaaactct gctgctgcca gccagtggtc
     1561 tctctgccct gcagatgagg agcagaggcg agccgcccac ctcaatgggc tacaggcatc
     1621 cacaggctct gctgtggcct gctcaacccc ggtgcagtac ctgtccccag aatgtagcga
     1681 acagcccctg cagaaccagc ctagccccac agctgggata ggggatgaag aacaccaaca
     1741 aattgtgcct cacacagagg tggtagacct caaggcccag ctgcaggtga tggagaactt
     1801 aatcagctct agccaggaga ccatcaaagt gctgctggga gttattcagg agctggagaa
     1861 aggagaagcc catcgggaag ggctttcata tcggacgggg caagacacag ctaattgtga
     1921 cacgtgcagg aacagtgcat gtattatcta tagtgtggag ctggatttta agcaacaaga
     1981 agacaaactg cagcctgtgc tgagaaaact gcaccctgtc gaggaaactc aggtcatccc
     2041 ttcgccttac cctcaggaga ctcactcctc cacgcccaag caaaaatcca aaactgagtc
     2101 caaaaagcat ggcaggtgga aactctggtt tctttaagac tcgtgggctc gggaagaccc
     2161 agaccgtctt ttaaacccac tgggatttaa gtgagtcctt ttccgaatcg gataggatca
     2221 aggaaactgc ggtgccaacg cgtgctccct gcttctcgcc ctgcgtacca aaaaggcctt
     2281 cgcaccaaca gataatcagc agcaagggag tgactgtcgc cccttgccag ctgtcctttg
     2341 cagttcactg gtatggggta taaagtctga aataaaactt acgtagtcaa agataaggaa
     2401 aaactctaac ccccgacagc gcccccttac ctaagaattt gtatcgagat gccagtgttc
     2461 catacggcag gctgtctttg tagacagtag aacttctcat tatttttggc tctttattat
     2521 ttttttttaa atgagaagag aaagcaagcc ttgtgttggc aaaggacttc atttttacct
     2581 ttcattatga aataagcagg ggcgagtgca gttttcagca cattcaattc cggagaaagc
     2641 acaatttttt ttcaagtggt cggagaccat gaataatctc gaaaaatgtg actctatgta
     2701 tatctgcctt tgtgtgctgt agcgctaagc caagtgacca catctcagtg tcaaattgac
     2761 accacagatt tagacccctc ttcatctcca gatggacgac atgtttactg ttcatttccc
     2821 aaatggccag cagccaggaa ccactcccac ccccaccccg agtcatgata tgccttcaat
     2881 cattgcaagc tgacagggtc agagctcctg gacactaagc tttcttagaa cttgttttta
     2941 ttctttgggt acccaaaggc caagtgatga atctggcaag aattgggaaa cagacagaga
     3001 tacacaatgg gtaatatagt tacctataaa tcacgggtgc ctaggctgcg gcagaaagcc
     3061 ctggttcttt tttttttttt ttttttcttt ttgtagctca atccccaggg atgttcttta
     3121 tccccaagcg agtgcagcca ggtgttttat atagttcttt atgctttgtc actactgtgt
     3181 gagatctgac catcgcagag agaaactgcc tgtttccctt agctgctacc aatggccaaa
     3241 cttaccattg cctccattta gcctatcaga aaattgtatt aaatggcata cttagggaga
     3301 agctgtgaat caggtggttt taggcatttt taaaaatctg ccgtataatc aagagttctc
     3361 tcttgatttc acgtgacaac gcgaacctcc accccccgtt tttccgctca tttctttgca
     3421 cctatacaag taccttttta acagaaacca aactcctcat gtgcattaat gacgtctctc
     3481 tgtgttctgc ttttcacgta ggaagtaggg gtcttccttt gccttttttc cccccttttc
     3541 gtataactta acgttttgaa tagtttgtaa gcataccaga aaaacctagt gttggtccag
     3601 cactttcagc tggaactgat gctgatggca gatcagactg caaacctgca gctgctcaat
     3661 ttcgggagag agtcctgtcc ttctgatggg taagagctaa ggctcaccac atgcttgtcc
     3721 tctggactga agagtttcat ttagttctaa acatttgagt tacacaacaa gctttaacag
     3781 aaaaaaagct aaaagaatat tttgtgtgaa gactctttgg gaactgctca caccacacta
     3841 gaggcgtgct taatttgaaa tgcaagggca ggtgcgtgcg tgcatgcacg cacaagacac
     3901 acatacacac acacatgcaa aacacacttg cgcacacaca cacacatgca cacaaacgtg
     3961 gaacatgcct atgtggaagt accctacagg aatacgaatc tgtagaagag cagtaaacag
     4021 gcctgtggga gaagagccct gaccaagctg atccggtacc ttgaaggaag tgcaattgtt
     4081 tcatgcatac taaactttaa aacacgtgtt tcatgttttt atactgtttt taactgatgt
     4141 tataaaaatg ttcacagttt tctcttcctg tgtttgaaga atcccatgtt ctcaacaagt
     4201 gaatggaagg gttcagcagc tttctgtcca gtgctacaca tctgtgctgc acatctgtct
     4261 ccatgggagc atatgtgatt acctgtgacg tcaatgtttc caaccaggct agggccccaa
     4321 ataaaaagat tggacatgtg aggaccagaa atgaaactct gaagatgaag caattcttcc
     4381 tcaaaagaaa agcatctccc caaactggaa acaaaatata ttggcaagct catttcatag
     4441 ctgaatcacg acctgccagt gacagacaga ggatgaccct tctcctggaa agattgagct
     4501 caaaagtttc atacaaaact aagtaaaaaa ttcagtgtcc cctcctatgt agatgtacac
     4561 atggcattac gtatagagag taaattatta tacttgtcat taaatattta ttaattttta
     4621 aataaaaaaa acaaagg
//
