LOCUS       NM_001290648            1109 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus insulin-like 5 (Insl5), transcript variant 1, mRNA.
ACCESSION   NM_001290648 XM_006503068
VERSION     NM_001290648.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Vahkal,B., Yegorov,S., Onyilagha,C., Donner,J., Reddick,D.,
            Shrivastav,A., Uzonna,J. and Good,S.V.
  TITLE     Immune System Effects of Insulin-Like Peptide 5 in a Mouse Model
  JOURNAL   Front Endocrinol (Lausanne) 11, 610672 (2021)
   PUBMED   33519716
  REMARK    GeneRIF: Immune System Effects of Insulin-Like Peptide 5 in a Mouse
            Model.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Kay,R.G., Galvin,S., Larraufie,P., Reimann,F. and Gribble,F.M.
  TITLE     Liquid chromatography/mass spectrometry based detection and
            semi-quantitative analysis of INSL5 in human and murine tissues
  JOURNAL   Rapid Commun Mass Spectrom 31 (23), 1963-1973 (2017)
   PUBMED   28857318
  REMARK    GeneRIF: Mass spectral analysis of purified enteroendocrine cells
            and tissue homogenates identified the exact sequence of A and B
            chains of INSL5 endogenously expressed in L-cells.
REFERENCE   3  (bases 1 to 1109)
  AUTHORS   Lee,Y.S., De Vadder,F., Tremaroli,V., Wichmann,A., Mithieux,G. and
            Backhed,F.
  TITLE     Insulin-like peptide 5 is a microbially regulated peptide that
            promotes hepatic glucose production
  JOURNAL   Mol Metab 5 (4), 263-270 (2016)
   PUBMED   27069866
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1109)
  AUTHORS   Grosse,J., Heffron,H., Burling,K., Akhter Hossain,M., Habib,A.M.,
            Rogers,G.J., Richards,P., Larder,R., Rimmington,D.,
            Adriaenssens,A.A., Parton,L., Powell,J., Binda,M., Colledge,W.H.,
            Doran,J., Toyoda,Y., Wade,J.D., Aparicio,S., Carlton,M.B.,
            Coll,A.P., Reimann,F., O'Rahilly,S. and Gribble,F.M.
  TITLE     Insulin-like peptide 5 is an orexigenic gastrointestinal hormone
  JOURNAL   Proc Natl Acad Sci U S A 111 (30), 11133-11138 (2014)
   PUBMED   25028498
  REMARK    GeneRIF: Insl5 is an orexigenic hormone released from colonic
            L-cells, which promotes appetite during conditions of energy
            deprivation.
REFERENCE   5  (bases 1 to 1109)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   6  (bases 1 to 1109)
  AUTHORS   Dun,S.L., Brailoiu,E., Wang,Y., Brailoiu,G.C., Liu-Chen,L.Y.,
            Yang,J., Chang,J.K. and Dun,N.J.
  TITLE     Insulin-like peptide 5: expression in the mouse brain and
            mobilization of calcium
  JOURNAL   Endocrinology 147 (7), 3243-3248 (2006)
   PUBMED   16601133
  REMARK    GeneRIF: Insulin-like peptide 5 is expressed in mouse brain and has
            a role in mobilization of calcium
REFERENCE   7  (bases 1 to 1109)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   8  (bases 1 to 1109)
  AUTHORS   Hsu,S.Y.
  TITLE     Cloning of two novel mammalian paralogs of relaxin/insulin family
            proteins and their expression in testis and kidney
  JOURNAL   Mol Endocrinol 13 (12), 2163-2174 (1999)
   PUBMED   10598589
REFERENCE   9  (bases 1 to 1109)
  AUTHORS   Berger,J., Kurahashi,H., Takihara,Y., Shimada,K., Brock,H.W. and
            Randazzo,F.
  TITLE     The human homolog of Sex comb on midleg (SCMH1) maps to chromosome
            1p34
  JOURNAL   Gene 237 (1), 185-191 (1999)
   PUBMED   10524249
REFERENCE   10 (bases 1 to 1109)
  AUTHORS   Conklin,D., Lofton-Day,C.E., Haldeman,B.A., Ching,A.,
            Whitmore,T.E., Lok,S. and Jaspers,S.
  TITLE     Identification of INSL5, a new member of the insulin superfamily
  JOURNAL   Genomics 60 (1), 50-56 (1999)
   PUBMED   10458910
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX004765.6 and AL732543.8.
            
            On Jun 19, 2019 this sequence version replaced NM_001290648.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.320092.1,
                                           SRR6116558.117650.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-458               BX004765.6         3323-3780           c
            459-634             BX004765.6         3027-3202           c
            635-1109            AL732543.8         187653-188127       c
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 47.27 cM"
     gene            1..1109
                     /gene="Insl5"
                     /gene_synonym="RIF2"
                     /note="insulin-like 5"
                     /db_xref="GeneID:23919"
                     /db_xref="MGI:MGI:1346085"
     exon            1..458
                     /gene="Insl5"
                     /gene_synonym="RIF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             430..867
                     /gene="Insl5"
                     /gene_synonym="RIF2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; insulin-like peptide INSL5;
                     relaxin/insulin-like protein; relaxin/insulin-like factor
                     2"
                     /codon_start=1
                     /product="insulin-like peptide INSL5 isoform 1 precursor"
                     /protein_id="NP_001277577.1"
                     /db_xref="CCDS:CCDS71431.1"
                     /db_xref="GeneID:23919"
                     /db_xref="MGI:MGI:1346085"
                     /translation="MCLVNTVKIRMKGPTLALFLLLVLLAVVEVRSRQTVKLCGLDYV
                     RTVIYICASSRWRRHLEGHFHSQQAETRNYLQLLDRHEPSKKTLEHSLPKTDLSGQEL
                     VRDPQAPKEGLWELKKHSVVSRRDLQALCCREGCSMKELSTLC"
     exon            459..634
                     /gene="Insl5"
                     /gene_synonym="RIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            635..1109
                     /gene="Insl5"
                     /gene_synonym="RIF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1084..1089
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Insl5"
                     /gene_synonym="RIF2"
                     /note="hexamer: AATAAA"
     polyA_site      1109
                     /gene="Insl5"
                     /gene_synonym="RIF2"
                     /note="major polyA site"
ORIGIN      
        1 ctttcgggat agcatttgaa atgtaaataa agaaaatatc taataaaaaa gaataaaaaa
       61 atagagtact taaacaaatt gctttagatt ctagatcttg tgggaggaaa gccaggaatc
      121 aaatctgagt ccgacagacc tcatcactgt gtgacttccc actgcattga catagaagct
      181 gcttctgctc ccgaagcctc ccatgctaga gagataccac tattgacgtt cagagtttaa
      241 agcttgttct aggtcagtca gcaaatgtaa agtgggcatt gaagcttatg catccctgct
      301 tccaaaagcc ttgtatttcc acagcgactc ctgagaagat gcagcaatgc taagtgagat
      361 gtgttcagat gaccgagtga gcagcaataa tcataaaacg cacatggctg gtcctccaca
      421 ctgtcaccta tgtgcttagt aaacactgtt aagataagga tgaagggccc cactcttgct
      481 ctgtttctcc tcttagttct gttggctgtg gtggaagtaa gaagcaggca gactgtgaag
      541 ctctgtggcc tggactacgt gagaacagtt atctacatct gtgccagctc acggtggagg
      601 agacatctgg aggggcattt ccactctcaa caagctgaga caagaaacta cctccagctc
      661 ctagacaggc acgagccatc caagaaaact ctggagcaca gccttcccaa gacggatctc
      721 tcaggacagg agcttgttcg agatccacag gcacccaagg aaggtctttg ggaactgaag
      781 aagcactcag tggtatccag acgagatctg caagctctgt gctgcaggga aggctgctcc
      841 atgaaggaac tcagcaccct ctgttaggat gcgcccaacc ccttggcagg cttcagcatg
      901 catctcaatg ttctaccatc gagttccctg ttcagcttct atcactacaa ccacggcttt
      961 tgatcctttc cttaaaggtc tattatggct taaagccact cttctccctg tgctgaggtc
     1021 aatctacttt tctttctaaa ttctaactac tgctttgaag tttcgagtgc tgtgcaaaat
     1081 tgcaataaaa aaaaatgcct gaatccaaa
//
