LOCUS       NM_013564                587 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus insulin-like 3 (Insl3), mRNA.
ACCESSION   NM_013564
VERSION     NM_013564.7
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Robert,N.M., Ferrier-Tarin,S. and Tremblay,J.J.
  TITLE     A New Leydig Cell-Exclusive Cre Line Allows Lineage Tracing of
            Fetal and Adult Leydig Cell Populations in the Mouse
  JOURNAL   Endocrinology 166 (2) (2025)
   PUBMED   39823408
REFERENCE   2  (bases 1 to 587)
  AUTHORS   Gu,X., Heinrich,A., Li,S.Y. and DeFalco,T.
  TITLE     Testicular macrophages are recruited during a narrow fetal time
            window and promote organ-specific developmental functions
  JOURNAL   Nat Commun 14 (1), 1439 (2023)
   PUBMED   36922518
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 587)
  AUTHORS   Giner,X.C., Pierre,K.J., Robert,N.M. and Tremblay,J.J.
  TITLE     A 35-bp Conserved Region Is Crucial for Insl3 Promoter Activity in
            Mouse MA-10 Leydig Cells
  JOURNAL   Int J Mol Sci 23 (23), 15060 (2022)
   PUBMED   36499388
  REMARK    GeneRIF: A 35-bp Conserved Region Is Crucial for Insl3 Promoter
            Activity in Mouse MA-10 Leydig Cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 587)
  AUTHORS   Prokopuk,L., Jarred,E.G., Blucher,R.O., McLaughlin,E.A.,
            Stringer,J.M. and Western,P.S.
  TITLE     An essential role for Polycomb Repressive Complex 2 in the mouse
            ovary
  JOURNAL   Reproduction 163 (3), 167-182 (2022)
   PUBMED   35084365
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 587)
  AUTHORS   Kothandapani,A., Lewis,S.R., Noel,J.L., Zacharski,A., Krellwitz,K.,
            Baines,A., Winske,S., Vezina,C.M., Kaftanovskaya,E.M.,
            Agoulnik,A.I., Merton,E.M., Cohn,M.J. and Jorgensen,J.S.
  TITLE     GLI3 resides at the intersection of hedgehog and androgen action to
            promote male sex differentiation
  JOURNAL   PLoS Genet 16 (6), e1008810 (2020)
   PUBMED   32497091
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 587)
  AUTHORS   Zimmermann,S., Steding,G., Emmen,J.M., Brinkmann,A.O., Nayernia,K.,
            Holstein,A.F., Engel,W. and Adham,I.M.
  TITLE     Targeted disruption of the Insl3 gene causes bilateral
            cryptorchidism
  JOURNAL   Mol Endocrinol 13 (5), 681-691 (1999)
   PUBMED   10319319
REFERENCE   7  (bases 1 to 587)
  AUTHORS   Koskimies,P., Spiess,A.N., Lahti,P., Huhtaniemi,I. and Ivell,R.
  TITLE     The mouse relaxin-like factor gene and its promoter are located
            within the 3' region of the JAK3 genomic sequence
  JOURNAL   FEBS Lett 419 (2-3), 186-190 (1997)
   PUBMED   9428631
REFERENCE   8  (bases 1 to 587)
  AUTHORS   Taketo,M.M., Araki,Y., Matsunaga,A., Yokoi,A., Tsuchida,J.,
            Nishina,Y., Nozaki,M., Tanaka,H., Koga,M., Uchida,K., Matsumiya,K.,
            Okuyama,A., Rochelle,J.M., Nishimune,Y., Matsui,M. and Seldin,M.F.
  TITLE     Mapping of eight testis-specific genes to mouse chromosomes
  JOURNAL   Genomics 46 (1), 138-142 (1997)
   PUBMED   9403069
REFERENCE   9  (bases 1 to 587)
  AUTHORS   Zimmermann,S., Schottler,P., Engel,W. and Adham,I.M.
  TITLE     Mouse Leydig insulin-like (Ley I-L) gene: structure and expression
            during testis and ovary development
  JOURNAL   Mol Reprod Dev 47 (1), 30-38 (1997)
   PUBMED   9110312
REFERENCE   10 (bases 1 to 587)
  AUTHORS   Pusch,W., Balvers,M. and Ivell,R.
  TITLE     Molecular cloning and expression of the relaxin-like factor from
            the mouse testis
  JOURNAL   Endocrinology 137 (7), 3009-3013 (1996)
   PUBMED   8770925
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132857.1 and AC162033.11.
            
            On Dec 22, 2011 this sequence version replaced NM_013564.6.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049540.1, CN842510.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-584               AK132857.1         2-585
            585-587             AC162033.11        146178-146180
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.43 cM"
     gene            1..587
                     /gene="Insl3"
                     /gene_synonym="Rlf; Rlnl"
                     /note="insulin-like 3"
                     /db_xref="GeneID:16336"
                     /db_xref="MGI:MGI:108427"
     exon            1..181
                     /gene="Insl3"
                     /gene_synonym="Rlf; Rlnl"
                     /inference="alignment:Splign:2.1.0"
     CDS             7..375
                     /gene="Insl3"
                     /gene_synonym="Rlf; Rlnl"
                     /note="ley-I-L; relaxin-like factor; leydig insulin-like
                     peptide; Ley I-L"
                     /codon_start=1
                     /product="insulin-like 3 precursor"
                     /protein_id="NP_038592.3"
                     /db_xref="CCDS:CCDS22404.1"
                     /db_xref="GeneID:16336"
                     /db_xref="MGI:MGI:108427"
                     /translation="MRAPLLLMLLALGSALRSPQPPEARAKLCGHHLVRTLVRVCGGP
                     RWSPEATQPVETRDRELLQWLEQRHLLHALVADVDPALDPQLPLQASQRQRRSAATNA
                     VHRCCLTGCTQQDLLGLCPH"
     exon            182..587
                     /gene="Insl3"
                     /gene_synonym="Rlf; Rlnl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      568..573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Insl3"
                     /gene_synonym="Rlf; Rlnl"
                     /note="hexamer: AATAAA"
     polyA_site      585
                     /gene="Insl3"
                     /gene_synonym="Rlf; Rlnl"
                     /note="major polyA site"
ORIGIN      
        1 gccaccatgc gcgcgccgct gctactgatg ctcctggctc tggggtccgc gctgcgctcc
       61 ccgcagcccc ccgaggcacg cgccaagctc tgcggccacc acctggtgcg cacgctggtg
      121 cgggtgtgcg gcggcccacg ctggtcgccc gaggccacgc agcctgtgga gacccgggac
      181 cgggagctgc tgcagtggct agagcagaga catctcctgc acgcgctggt ggccgacgtg
      241 gacccagcgc tagacccgca gcttcctctc caggcttctc agcgccagcg ccgcagtgcc
      301 gccaccaacg ctgtgcaccg ctgctgtctt actggctgca cccagcaaga cctgttgggt
      361 ctgtgtcccc actgaagggg cacagggctc aggcggtgcg tccttaattg ctccccacct
      421 cctggctatg tcattgcaac aatccagttt gccttggagg atccctagtt tattcataga
      481 tcgcagtaag caaggaccac aggccctgtc cccaagaagc cccatcatga cctgtgccca
      541 cctcctccct gtgtcccaaa aagtctaaat aaaaaagcaa cttcata
//
