LOCUS       NM_133748               2711 bp    mRNA    linear   ROD 12-JUL-2024
DEFINITION  Mus musculus insulin induced gene 2 (Insig2), transcript variant 1,
            mRNA.
ACCESSION   NM_133748
VERSION     NM_133748.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wu,Y., Li,C., Khan,A.A., Chen,K., Su,R., Xu,S., Sun,Y., Gao,F.,
            Wang,K., Wang,X., Lian,Z., Wang,S., Yu,M., Hu,X., Yang,F.,
            Zheng,S., Qiu,N., Liu,Z. and Xu,X.
  TITLE     Insulin-induced gene 2 protects against hepatic
            ischemia-reperfusion injury via metabolic remodeling
  JOURNAL   J Transl Med 21 (1), 739 (2023)
   PUBMED   37858181
  REMARK    GeneRIF: Insulin-induced gene 2 protects against hepatic
            ischemia-reperfusion injury via metabolic remodeling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2711)
  AUTHORS   Zeng,H., Qin,H., Liao,M., Zheng,E., Luo,X., Xiao,A., Li,Y.,
            Chen,L., Wei,L., Zhao,L., Ruan,X.Z., Yang,P. and Chen,Y.
  TITLE     CD36 promotes de novo lipogenesis in hepatocytes through
            INSIG2-dependent SREBP1 processing
  JOURNAL   Mol Metab 57, 101428 (2022)
   PUBMED   34974159
  REMARK    GeneRIF: CD36 promotes de novo lipogenesis in hepatocytes through
            INSIG2-dependent SREBP1 processing.
REFERENCE   4  (bases 1 to 2711)
  AUTHORS   Zhou,Z.S., Li,M.X., Liu,J., Jiao,H., Xia,J.M., Shi,X.J., Zhao,H.,
            Chu,L., Liu,J., Qi,W., Luo,J. and Song,B.L.
  TITLE     Competitive oxidation and ubiquitylation on the evolutionarily
            conserved cysteine confer tissue-specific stabilization of Insig-2
  JOURNAL   Nat Commun 11 (1), 379 (2020)
   PUBMED   31953408
  REMARK    GeneRIF: Insig-2 is an ER membrane protein negatively controlling
            lipid biosynthesis. The stabilized Insig-2 downregulates
            lipogenesis through inhibiting the SREBP pathway, helping to
            channel the carbon flux to ATP generation and protecting myotubes
            from lipid over-accumulation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2711)
  AUTHORS   Suzuki,A., Shim,J., Ogata,K., Yoshioka,H. and Iwata,J.
  TITLE     Cholesterol metabolism plays a crucial role in the regulation of
            autophagy for cell differentiation of granular convoluted tubules
            in male mouse submandibular glands
  JOURNAL   Development 146 (20) (2019)
   PUBMED   31558435
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2711)
  AUTHORS   Engelking,L.J., Liang,G., Hammer,R.E., Takaishi,K., Kuriyama,H.,
            Evers,B.M., Li,W.P., Horton,J.D., Goldstein,J.L. and Brown,M.S.
  TITLE     Schoenheimer effect explained--feedback regulation of cholesterol
            synthesis in mice mediated by Insig proteins
  JOURNAL   J Clin Invest 115 (9), 2489-2498 (2005)
   PUBMED   16100574
  REMARK    GeneRIF: Results define Insig proteins 1 and 2 as essential
            elements of the feedback inhibition system of cholesterol synthesis
            in mouse liver.
REFERENCE   7  (bases 1 to 2711)
  AUTHORS   Lee,S., Lee,D.K., Choi,E. and Lee,J.W.
  TITLE     Identification of a functional vitamin D response element in the
            murine Insig-2 promoter and its potential role in the
            differentiation of 3T3-L1 preadipocytes
  JOURNAL   Mol Endocrinol 19 (2), 399-408 (2005)
   PUBMED   15528275
  REMARK    GeneRIF: a vitamin D response element in the murine Insig-2
            promoter may have a role in the differentiation of 3T3-L1
            preadipocytes
REFERENCE   8  (bases 1 to 2711)
  AUTHORS   Cheverud,J.M., Ehrich,T.H., Hrbek,T., Kenney,J.P., Pletscher,L.S.
            and Semenkovich,C.F.
  TITLE     Quantitative trait loci for obesity- and diabetes-related traits
            and their dietary responses to high-fat feeding in LGXSM
            recombinant inbred mouse strains
  JOURNAL   Diabetes 53 (12), 3328-3336 (2004)
   PUBMED   15561968
REFERENCE   9  (bases 1 to 2711)
  AUTHORS   Yabe,D., Komuro,R., Liang,G., Goldstein,J.L. and Brown,M.S.
  TITLE     Liver-specific mRNA for Insig-2 down-regulated by insulin:
            implications for fatty acid synthesis
  JOURNAL   Proc Natl Acad Sci U S A 100 (6), 3155-3160 (2003)
   PUBMED   12624180
  REMARK    GeneRIF: Liver-specific mRNA for Insig-2 down-regulated by insulin:
            implications for fatty acid synthesis.
REFERENCE   10 (bases 1 to 2711)
  AUTHORS   Yabe,D., Brown,M.S. and Goldstein,J.L.
  TITLE     Insig-2, a second endoplasmic reticulum protein that binds SCAP and
            blocks export of sterol regulatory element-binding proteins
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 12753-12758 (2002)
   PUBMED   12242332
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163333.6 and BC023067.1.
            
            On Oct 12, 2012 this sequence version replaced NM_133748.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1). Variants 1, 2 and 3
            encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC023067.1, AK152076.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-262               AC163333.6         98917-99178         c
            263-2711            BC023067.1         1-2449
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 52.91 cM"
     gene            1..2711
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /note="insulin induced gene 2"
                     /db_xref="GeneID:72999"
                     /db_xref="MGI:MGI:1920249"
     exon            1..334
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /inference="alignment:Splign:2.1.0"
     exon            335..710
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             467..1144
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     INSIG-2 membrane protein; insulin-induced gene 2 protein"
                     /codon_start=1
                     /product="insulin-induced gene 2 protein isoform 1"
                     /protein_id="NP_598509.1"
                     /db_xref="CCDS:CCDS15236.1"
                     /db_xref="GeneID:72999"
                     /db_xref="MGI:MGI:1920249"
                     /translation="MAEGETESPRPKKCGPYISSVTSQSVNVVIRGVVLFFIGVFLAL
                     VLNLLQIQRNVTLFPPDVITSIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFK
                     REWSSVMRCVAVFVGINHASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVG
                     IAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAMYECKVI
                     AEKSHQE"
     exon            711..835
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /inference="alignment:Splign:2.1.0"
     exon            836..1002
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1102
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..2697
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1337..1342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /note="hexamer: AATAAA"
     polyA_site      1362
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
     regulatory      2676..2681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2697
                     /gene="Insig2"
                     /gene_synonym="2900053I11Rik; C730043J18Rik; Insig-2"
                     /note="major polyA site"
ORIGIN      
        1 acagagtcca gatgcccatc cagaacctct gactcccgag ggcaccaaca acagctgatt
       61 tcgcagcctc tcctcctccc agacagggtc cccacctcct tccagcccgg agtgcccggc
      121 acaccggaag tccttttgcc ccgtctccgc agagcggagg cagtgcgcgc agaggcaacc
      181 ggaagctcct ctgcccctac cgtcagcgtg aagccgccgg gcggcgcagg cgcagtgcgg
      241 ctcaggcgga gctgtgggag gggagtaggt cgggggccgg tccctgaaga tggcggaggc
      301 gggggtgaac tgggggtctc cgggcagagc tcaggatttc tggtaggtcc cacgttcaga
      361 caagatgtac cattgaaaca ttggtctgct tcacttcaca gttcaccttt tccactggga
      421 agccacattt tgttttacgg cttaggccta ctgaacttat gaaaccatgg cggaaggaga
      481 gacggagtca cctcggccta aaaagtgtgg cccatacatt tcctctgtca ccagccagag
      541 tgtgaacgtg gtgatccgcg gggtggtact cttcttcatt ggagtgttcc tggcattagt
      601 gttgaacttg cttcagattc agagaaacgt gacacttttt ccaccagatg tgatcacgag
      661 catcttttca tctgcctggt gggtaccacc atgctgcggc acagcctcag ctgtgattgg
      721 gctgttgtac ccctgcattg acaggcatct aggagaacct cataaattta aaagagagtg
      781 gtccagtgtc atgcgctgcg tggcggtgtt cgtgggtata aatcacgcca gtgctaaagt
      841 agacttcgac aacaacttcc agttttccct cacactggct gcactgtcag taggactgtg
      901 gtggactttt gacagatcta gaagtggttt tggccttggt gttggaattg ctttcttagc
      961 aaccgttgtc acccaactgt tagtctacaa tggtgtttat caatacacat ctccagattt
     1021 tctctatgtc cgttcttggt tgccatgtat attttttgct ggaggcataa cgatgggaaa
     1081 cattggccgt caactggcaa tgtatgaatg caaagttatt gctgaaaaat ctcatcaaga
     1141 atgaagaggg cagacgtctt atctgttctt tgggagcctt gggaaagcac cgtgggtatg
     1201 agaggttcac caacggagga cccagttttc ccttgattgt gtatgtgtgt ggtgtgtgtg
     1261 tgtgtgtgac acacatgttt ttgcagtctg tgattgcttc tgtaaatcag ttaccaacct
     1321 ttcggtattt gatttcaata aatgtaagat atctgtgcgc tacatgaaag atatgtttat
     1381 gagtacatga aatttctaag ttttttgaac acagcataca ttgtatcaca atgtcaatgt
     1441 gccaaacgtg ttactgacac agagaataag aatgctttga acaatttaga aatgtaatga
     1501 aagagaaaaa gaaagctaaa aacaaagcaa atagaaagca aattatagct ggccgttccc
     1561 tctcaccggc tggtgtgcct tttcccgttt ctaatcagct gtgttcgtca cagcacccac
     1621 atgtgttgtc tctgttgcta tcgtttcaga gtttgagtct tatatggtac tcatgaactt
     1681 ccatagaatg gttttaagtc tgcattgttg tagggaccca aagaatactt tcccgatttc
     1741 ttcagaaggc tgtgtgatgg gcagaggtgg ttatcaacat caccccactt tgagtctaca
     1801 tttggaaaaa acaaatgact aaaatcaggg gctttgttta gatgaatgtg cttgggaata
     1861 ctgtgtagcc ctctctctgc cacagaggcc aaagtgatgc taattgtcac tggctactga
     1921 agctcacagc tccagtgcat ggatgacagg aacacaggca gatcaaggtg acaggtcata
     1981 cagaacctat tggactctag aaagcaggcc atgtgactga gcagctatag agggcctgag
     2041 tgtgtggcct tcatcagtgc agtcggaaga tgctggaacc tgactccctg tgtgctaatg
     2101 tctattacaa ggtctgtcct tggagagcgt atggagagca cacttcacac atcccgttaa
     2161 caacgcctgc catgtattta tgggttaagt cgatcagctg actctccgct cgctttgcat
     2221 ttctgaaatt gttaattgac tggcacacaa gggtaaattc ttctgactac tgtggtgtgc
     2281 gtgtgcttct gttttgagtg tgagagaaaa tgtattcatg tgtctgtgtg tgcatgtgcc
     2341 catgctttcc tatagtgtca ggtagacatt ttaatttctt ttaaagcagt gctgtcagac
     2401 tgaaatcttg tacactggag tgtaatgtgc tttgtgttct caggcgcaga gtgtgaacat
     2461 cccaagagta ctccatactg aatatgtata cacaagccta atggtagtag tggtgaccac
     2521 actactctat ttattattct gtctttcaga tgttattcat ttagaacaaa taaggtatat
     2581 ttttagaatc aaatttgtaa acactataaa atctttaata agttataagg tctatgatat
     2641 gtttactttg aaaattgctg ttgaaagcaa gatgtattaa atatgtaatt atcaccaaaa
     2701 aaaaaaaaaa a
//
