LOCUS       NM_001405688            2326 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus INSC spindle orientation adaptor protein (Insc),
            transcript variant 1, mRNA.
ACCESSION   NM_001405688 XM_006507659
VERSION     NM_001405688.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Lau,E.O., Damiani,D., Chehade,G., Ruiz-Reig,N., Saade,R.,
            Jossin,Y., Aittaleb,M., Schakman,O., Tajeddine,N., Gailly,P. and
            Tissir,F.
  TITLE     DIAPH3 deficiency links microtubules to mitotic errors, defective
            neurogenesis, and brain dysfunction
  JOURNAL   Elife 10, e61974 (2021)
   PUBMED   33899739
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Yorgan,T.A., Peters,S., Amling,M. and Schinke,T.
  TITLE     Osteoblast-specific expression of Panx3 is dispensable for
            postnatal bone remodeling
  JOURNAL   Bone 127, 155-163 (2019)
   PUBMED   31202927
REFERENCE   3  (bases 1 to 2326)
  AUTHORS   Harris,L., Zalucki,O., Gobius,I., McDonald,H., Osinki,J.,
            Harvey,T.J., Essebier,A., Vidovic,D., Gladwyn-Ng,I., Burne,T.H.,
            Heng,J.I., Richards,L.J., Gronostajski,R.M. and Piper,M.
  TITLE     Transcriptional regulation of intermediate progenitor cell
            generation during hippocampal development
  JOURNAL   Development 143 (24), 4620-4630 (2016)
   PUBMED   27965439
  REMARK    Erratum:[Development. 2018 Jul 24;145(14):dev169631. doi:
            10.1242/dev.169631.. PMID: 30042194]
REFERENCE   4  (bases 1 to 2326)
  AUTHORS   Tarchini,B., Tadenev,A.L., Devanney,N. and Cayouette,M.
  TITLE     A link between planar polarity and staircase-like bundle
            architecture in hair cells
  JOURNAL   Development 143 (21), 3926-3932 (2016)
   PUBMED   27660326
REFERENCE   5  (bases 1 to 2326)
  AUTHORS   Ishibashi,R., Kozuki,S., Kamakura,S., Sumimoto,H. and Toyoshima,F.
  TITLE     c-Rel Regulates Inscuteable Gene Expression during Mouse Embryonic
            Stem Cell Differentiation
  JOURNAL   J Biol Chem 291 (7), 3333-3345 (2016)
   PUBMED   26694615
  REMARK    GeneRIF: Data (including data from studies using embryonic stem
            cell line) suggest that expression of Insc (inscuteable cell cycle
            protein) is up-regulated by c-Rel in cell differentiation; minimum
            transcriptional regulatory element (354 bases) was identified.
REFERENCE   6  (bases 1 to 2326)
  AUTHORS   El-Hashash,A.H. and Warburton,D.
  TITLE     Cell polarity and spindle orientation in the distal epithelium of
            embryonic lung
  JOURNAL   Dev Dyn 240 (2), 441-445 (2011)
   PUBMED   21246661
REFERENCE   7  (bases 1 to 2326)
  AUTHORS   Poulson,N.D. and Lechler,T.
  TITLE     Robust control of mitotic spindle orientation in the developing
            epidermis
  JOURNAL   J Cell Biol 191 (5), 915-922 (2010)
   PUBMED   21098114
REFERENCE   8  (bases 1 to 2326)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   9  (bases 1 to 2326)
  AUTHORS   Zigman,M., Cayouette,M., Charalambous,C., Schleiffer,A.,
            Hoeller,O., Dunican,D., McCudden,C.R., Firnberg,N., Barres,B.A.,
            Siderovski,D.P. and Knoblich,J.A.
  TITLE     Mammalian inscuteable regulates spindle orientation and cell fate
            in the developing retina
  JOURNAL   Neuron 48 (4), 539-545 (2005)
   PUBMED   16301171
REFERENCE   10 (bases 1 to 2326)
  AUTHORS   Lechler,T. and Fuchs,E.
  TITLE     Asymmetric cell divisions promote stratification and
            differentiation of mammalian skin
  JOURNAL   Nature 437 (7056), 275-280 (2005)
   PUBMED   16094321
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110186.10 and AC102792.7.
            
            On Apr 29, 2022 this sequence version replaced XM_006507659.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849382 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-250               AC110186.10        35975-36224         c
            251-351             AC110186.10        10919-11019         c
            352-697             AC102792.7         31615-31960
            698-750             AC102792.7         32909-32961
            751-874             AC102792.7         34633-34756
            875-988             AC102792.7         45142-45255
            989-1114            AC102792.7         52266-52391
            1115-1286           AC102792.7         69697-69868
            1287-1465           AC102792.7         75416-75594
            1466-1532           AC102792.7         82786-82852
            1533-1688           AC102792.7         85680-85835
            1689-1765           AC102792.7         87115-87191
            1766-2326           AC102792.7         90479-91039
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 60.23 cM"
     gene            1..2326
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /note="INSC spindle orientation adaptor protein"
                     /db_xref="GeneID:233752"
                     /db_xref="MGI:MGI:1917942"
     exon            1..250
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..1894
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein inscuteable homolog; minsc"
                     /codon_start=1
                     /product="protein inscuteable homolog isoform 1"
                     /protein_id="NP_001392617.1"
                     /db_xref="GeneID:233752"
                     /db_xref="MGI:MGI:1917942"
                     /translation="MRRPPGDGDSTGEGPGNWGLWGAQESRRLCCVGPDRCGQALLQI
                     GINMMALPGGRHLDSVPLQEQRLHFMQVDSVQRWMEDLKLMTECECMCVLQAKPISLE
                     EDTQGDLILAGGPGPGDPLQLLLKRGWVISTELRRIGQKLAQDRWARVHSMSVRLTCH
                     ARSMVSEYSTISRTASQEMGQAEKLLMEKCSELSAVTERCLQVENEHVLKSMKACVSE
                     TLSLLGEHFGQLLELALTREVQALVRKIDTSDNIYITESTTGNLFGLTQEGAPLCRII
                     AKEGGVVALFKVCRQDSFRCLYPQALRTLASICCVEEGVHQLEKVDGILCLADILTDE
                     SHSEATRAEAAAVVAQVTSPHLSFTQHLTSFLENMEEIVTALIKLCQEASSGEVFLLA
                     SAALANITFFDKMACEMLLQLNAIRVLLEACGDKQRVDTPYTRDQIVTILANMSVLEQ
                     CGSDIIQENGVQLIMGMLSEKPRSGTPAEVAACERVQQKAAVTLARLCRDPDVAQEAV
                     RLSCMSRLIELCRSPSERNSSDAVLVACLAALRRLAGVCPEGLQDSDFQQLVQPRLVD
                     SFLLCSNMEESFV"
     exon            251..351
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            352..697
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            698..750
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            751..874
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            875..988
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1114
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1286
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1465
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1532
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1688
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1765
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..2326
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2307..2312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /note="hexamer: AATAAA"
     polyA_site      2326
                     /gene="Insc"
                     /gene_synonym="3830422K02Rik; C730020D23"
                     /note="major polyA site"
ORIGIN      
        1 agttctgagc agccttgaag ttctgctcat tagcctttgc tttgaatctc aggccttctc
       61 aggtccatgg cccagaagga tgggtggaga gaatgaagat gctcacaggg gactctgtaa
      121 gaatacctga atcattacag cccagactat aaccatgagg cggcccccag gggatggaga
      181 ctccaccgga gagggcccag gcaactgggg cttgtgggga gcacaggagt ccaggaggct
      241 gtgctgtgtt ggtccggacc gctgtgggca ggcattgctg cagattggga ttaacatgat
      301 ggcactgcct ggaggccgcc acctggattc cgttcctctg caagagcaga ggctacactt
      361 catgcaggta gactcggttc agcgctggat ggaggatctg aagctcatga ccgagtgtga
      421 gtgcatgtgc gtcttgcagg caaagcccat cagcctggag gaagacacgc aaggtgacct
      481 tatcctagca ggtggtcctg gtccaggaga tcctctacag ctgctcttga aacggggctg
      541 ggtcatcagc accgagcttc gaaggatcgg gcagaagctg gcccaggacc gctgggcccg
      601 tgtgcacagc atgagtgtgc gtctgacgtg ccatgcacgc tccatggtca gcgagtacag
      661 caccatcagc aggacagcct cacaggaaat gggccaggcg gagaagctgc tcatggagaa
      721 atgctctgag ctctctgcgg tcacggagag gtgcctccag gtggaaaatg agcatgttct
      781 gaagtcaatg aaggcctgcg tgagtgagac cctgagcctg ctgggcgagc actttggtca
      841 gctactggag ctggccctca cacgggaagt gcaggcactt gtgagaaaaa tcgatacctc
      901 agacaatatc tacatcacgg agtctaccac agggaatctg ttcggcctga cccaggaggg
      961 ggctcctctg tgccgcatca tagccaagga aggtggggtt gtagccctct tcaaggtctg
     1021 ccggcaggac agcttccggt gcttgtaccc ccaggcactc cgcacactgg cctctatctg
     1081 ctgtgtagaa gagggtgtcc atcagctgga gaaggtggac ggtatcctgt gcttggctga
     1141 catcttgact gatgagagcc actcggaggc cactagagct gaggctgcag ccgtggtagc
     1201 ccaggttacc tccccacatc tgtccttcac ccagcacctc actagcttcc tggagaacat
     1261 ggaggagatt gtgacagccc ttatcaaact gtgccaagag gcctcgtcag gggaagtctt
     1321 cctgctggcc tctgcggccc tcgccaatat caccttcttc gacaaaatgg cctgtgagat
     1381 gcttctacag ttgaatgcaa tccgtgtcct cctggaagcc tgtggtgaca agcagagagt
     1441 ggacacccct tatactcggg accagattgt gaccatcttg gcaaacatgt ctgtgctgga
     1501 gcagtgtggc tctgacatca ttcaggagaa cggtgtccag ctcatcatgg gcatgctgtc
     1561 tgagaagcca aggtctggga cacctgctga agtggcagcc tgtgaaagag tacagcagaa
     1621 agctgcagtg accctggccc gcctctgccg agacccagat gtggcccagg aggccgttcg
     1681 gctcagctgt atgtctcgcc tgattgagct ctgccggtcc ccctctgaga ggaacagtag
     1741 tgatgctgtc ctggtggcct gcctggctgc cctgcgtcgg ttggccgggg tctgcccgga
     1801 aggccttcag gactctgact tccagcagct ggtccagcct cggctggtgg attccttctt
     1861 actgtgtagc aatatggagg agagttttgt gtagtgagca tgagagagaa ggcacactgt
     1921 gtgcacttac cacgctagct ctcttcagct ccttatttat atgtaattta ttttttacat
     1981 gaaatagaaa gggaatcttt atcagtgagt agctggtcct cggggacccc taccccccca
     2041 cttctgtgac taggtgcaga agttgaattg tgtcaaggct aacatgacag gttgtcacag
     2101 gtgactcccc ttcatactgc tccctctctt ctcagcatat attcttgcct gtttaaaaaa
     2161 caagacaaaa caaaacaaaa aagcccaact ttgaaccagg gcattttaac tgcccagcat
     2221 tttttaaaag ttagttttac tcttttgaga aatttaaatt gaattttgca gtttgaaaga
     2281 tatactgtaa gaaaatgttt ttctttaata aatggaatca tggaaa
//
