LOCUS       NM_001185083             473 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus insulin II (Ins2), transcript variant 1, mRNA.
ACCESSION   NM_001185083
VERSION     NM_001185083.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Scavuzzo,M.A., Szlachcic,W.J., Hill,M.C., Ziojla,N.M., Teaw,J.,
            Carlson,J.C., Tiessen,J., Chmielowiec,J., Martin,J.F. and
            Borowiak,M.
  TITLE     Pancreatic organogenesis mapped through space and time
  JOURNAL   Exp Mol Med 57 (1), 204-220 (2025)
   PUBMED   39779976
REFERENCE   2  (bases 1 to 473)
  AUTHORS   Lin,H.H., Yu,I.S., Cheng,M.S., Chang,T.J., Lin,H.Y., Chang,Y.C.,
            Ko,C.J., Chen,P.H., Lin,S.W., Huang,T.C., Huang,S.Y., Chen,T.Y.,
            Kan,K.W., Huang,H.P. and Lee,M.S.
  TITLE     Spint1 disruption in mouse pancreas leads to glucose intolerance
            and impaired insulin production involving HEPSIN/MAFA
  JOURNAL   Nat Commun 15 (1), 10537 (2024)
   PUBMED   39627229
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 473)
  AUTHORS   Li,Y., Zheng,R., Jiang,L., Yan,C., Liu,R., Chen,L., Jin,W., Luo,Y.,
            Zhang,X., Tang,J., Dai,Z. and Jiang,W.
  TITLE     A noncoding variant confers pancreatic differentiation defect and
            contributes to diabetes susceptibility by recruiting RXRA
  JOURNAL   Nat Commun 15 (1), 9771 (2024)
   PUBMED   39532884
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 473)
  AUTHORS   Panda,A.C., Kulkarni,S.D., Muralidharan,B., Bakthavachalu,B. and
            Seshadri,V.
  TITLE     Novel splice variant of mouse insulin2 mRNA: implications for
            insulin expression
  JOURNAL   FEBS Lett 584 (6), 1169-1173 (2010)
   PUBMED   20153322
  REMARK    GeneRIF: Alternative splicing of insulin mRNA in mice could result
            in an additional level of regulation in insulin biosynthesis.
REFERENCE   5  (bases 1 to 473)
  AUTHORS   Minn,A.H., Lan,H., Rabaglia,M.E., Harlan,D.M., Peculis,B.A.,
            Attie,A.D. and Shalev,A.
  TITLE     Increased insulin translation from an insulin splice-variant
            overexpressed in diabetes, obesity, and insulin resistance
  JOURNAL   Mol Endocrinol 19 (3), 794-803 (2005)
   PUBMED   15550470
REFERENCE   6  (bases 1 to 473)
  AUTHORS   Zemel,S., Bartolomei,M.S. and Tilghman,S.M.
  TITLE     Physical linkage of two mammalian imprinted genes, H19 and
            insulin-like growth factor 2
  JOURNAL   Nat Genet 2 (1), 61-65 (1992)
   PUBMED   1303252
REFERENCE   7  (bases 1 to 473)
  AUTHORS   Rappolee,D.A., Sturm,K.S., Behrendtsen,O., Schultz,G.A.,
            Pedersen,R.A. and Werb,Z.
  TITLE     Insulin-like growth factor II acts through an endogenous growth
            pathway regulated by imprinting in early mouse embryos
  JOURNAL   Genes Dev 6 (6), 939-952 (1992)
   PUBMED   1317321
REFERENCE   8  (bases 1 to 473)
  AUTHORS   Sawa,T., Ohgaku,S., Morioka,H. and Yano,S.
  TITLE     Molecular cloning and DNA sequence analysis of preproinsulin genes
            in the NON mouse, an animal model of human non-obese,
            non-insulin-dependent diabetes mellitus
  JOURNAL   J Mol Endocrinol 5 (1), 61-67 (1990)
   PUBMED   2397023
REFERENCE   9  (bases 1 to 473)
  AUTHORS   Wentworth,B.M., Schaefer,I.M., Villa-Komaroff,L. and Chirgwin,J.M.
  TITLE     Characterization of the two nonallelic genes encoding mouse
            preproinsulin
  JOURNAL   J Mol Evol 23 (4), 305-312 (1986)
   PUBMED   3104603
REFERENCE   10 (bases 1 to 473)
  AUTHORS   Bunzli,H.F., Glatthaar,B., Kunz,P., Mulhaupt,E. and Humbel,R.E.
  TITLE     Amino acid sequence of the two insulins from mouse (Maus musculus)
  JOURNAL   Hoppe Seylers Z Physiol Chem 353 (3), 451-458 (1972)
   PUBMED   5063718
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP775562.1 and BC099934.1.
            
            On Aug 13, 2015 this sequence version replaced NM_001185083.1.
            
            Summary: This gene encodes insulin, a peptide hormone that plays a
            vital role in the regulation of carbohydrate and lipid metabolism.
            The encoded precursor protein undergoes proteolytic cleavage to
            produce a disulfide-linked heterodimeric functional protein that is
            stored in secretory granules. An increase in blood glucose levels,
            among others, induces the release of insulin from the secretory
            granules. Mice deficient in the functional hormone encoded by this
            gene develop diabetes mellitus. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript. Variants 1-3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP775727.1, BG310959.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                BP775562.1         484-516             c
            34-473              BC099934.1         1-440
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 88.0 cM"
     gene            1..473
                     /gene="Ins2"
                     /gene_synonym="Ins-2; InsII; Mody; Mody4"
                     /note="insulin II"
                     /db_xref="GeneID:16334"
                     /db_xref="MGI:MGI:96573"
     exon            1..65
                     /gene="Ins2"
                     /gene_synonym="Ins-2; InsII; Mody; Mody4"
                     /inference="alignment:Splign:2.1.0"
     exon            66..266
                     /gene="Ins2"
                     /gene_synonym="Ins-2; InsII; Mody; Mody4"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..412
                     /gene="Ins2"
                     /gene_synonym="Ins-2; InsII; Mody; Mody4"
                     /note="insulin-2; proinsulin"
                     /codon_start=1
                     /product="insulin-2 preproprotein"
                     /protein_id="NP_001172012.1"
                     /db_xref="CCDS:CCDS22034.1"
                     /db_xref="GeneID:16334"
                     /db_xref="MGI:MGI:96573"
                     /translation="MALWMRFLPLLALLFLWESHPTQAFVKQHLCGSHLVEALYLVCG
                     ERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVAQQKRGIVDQCCTSICSL
                     YQLENYCN"
     exon            267..469
                     /gene="Ins2"
                     /gene_synonym="Ins-2; InsII; Mody; Mody4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      446..451
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ins2"
                     /gene_synonym="Ins-2; InsII; Mody; Mody4"
                     /note="hexamer: AATAAA"
     polyA_site      469
                     /gene="Ins2"
                     /gene_synonym="Ins-2; InsII; Mody; Mody4"
                     /note="major polyA site"
ORIGIN      
        1 ggggacccag taaccaccag ccctaagtga tccgctacaa tcaaaaacca tcagcaagca
       61 ggaaggttat tgtttcaaca tggccctgtg gatgcgcttc ctgcccctgc tggccctgct
      121 cttcctctgg gagtcccacc ccacccaggc ttttgtcaag cagcaccttt gtggttccca
      181 cctggtggag gctctctacc tggtgtgtgg ggagcgtggc ttcttctaca cacccatgtc
      241 ccgccgtgaa gtggaggacc cacaagtggc acaactggag ctgggtggag gcccgggagc
      301 aggtgacctt cagaccttgg cactggaggt ggcccagcag aagcgtggca ttgtagatca
      361 gtgctgcacc agcatctgct ccctctacca gctggagaac tactgcaact agacccacca
      421 ctacccagcc tacccctctg caatgaataa aacctttgaa tgagcacaaa aaa
//
