LOCUS       NM_008386                611 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus insulin I (Ins1), mRNA.
ACCESSION   NM_008386
VERSION     NM_008386.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Scavuzzo,M.A., Szlachcic,W.J., Hill,M.C., Ziojla,N.M., Teaw,J.,
            Carlson,J.C., Tiessen,J., Chmielowiec,J., Martin,J.F. and
            Borowiak,M.
  TITLE     Pancreatic organogenesis mapped through space and time
  JOURNAL   Exp Mol Med 57 (1), 204-220 (2025)
   PUBMED   39779976
REFERENCE   2  (bases 1 to 611)
  AUTHORS   Austin,M.C., Muralidharan,C., Roy,S., Crowder,J.J., Piganelli,J.D.
            and Linnemann,A.K.
  TITLE     Dysfunctional beta-cell autophagy induces beta-cell stress and
            enhances islet immunogenicity
  JOURNAL   Front Immunol 16, 1504583 (2025)
   PUBMED   39944686
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 611)
  AUTHORS   Lin,H.H., Yu,I.S., Cheng,M.S., Chang,T.J., Lin,H.Y., Chang,Y.C.,
            Ko,C.J., Chen,P.H., Lin,S.W., Huang,T.C., Huang,S.Y., Chen,T.Y.,
            Kan,K.W., Huang,H.P. and Lee,M.S.
  TITLE     Spint1 disruption in mouse pancreas leads to glucose intolerance
            and impaired insulin production involving HEPSIN/MAFA
  JOURNAL   Nat Commun 15 (1), 10537 (2024)
   PUBMED   39627229
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 611)
  AUTHORS   Kitamura,R.A., Hummel,D., Ustione,A., Piston,D.W. and Urano,F.
  TITLE     Dual role of neuroplastin in pancreatic beta cells: Regulating
            insulin secretion and promoting islet inflammation
  JOURNAL   Proc Natl Acad Sci U S A 121 (33), e2411234121 (2024)
   PUBMED   39666939
REFERENCE   5  (bases 1 to 611)
  AUTHORS   Rochelle,J.M., Watson,M.L., Oakey,R.J. and Seldin,M.F.
  TITLE     A linkage map of mouse chromosome 19: definition of comparative
            mapping relationships with human chromosomes 10 and 11 including
            the MEN1 locus
  JOURNAL   Genomics 14 (1), 26-31 (1992)
   PUBMED   1358795
REFERENCE   6  (bases 1 to 611)
  AUTHORS   Gittes,G.K. and Rutter,W.J.
  TITLE     Onset of cell-specific gene expression in the developing mouse
            pancreas
  JOURNAL   Proc Natl Acad Sci U S A 89 (3), 1128-1132 (1992)
   PUBMED   1371010
REFERENCE   7  (bases 1 to 611)
  AUTHORS   Mori,M., Ishizaki,K., Serikawa,T. and Yamada,J.
  TITLE     Localization of the rat insulin I gene (INS1) to chromosome 1q55 by
            fluorescence in situ hybridization
  JOURNAL   Cytogenet Cell Genet 59 (1), 31-33 (1992)
   PUBMED   1346376
REFERENCE   8  (bases 1 to 611)
  AUTHORS   Sawa,T., Ohgaku,S., Morioka,H. and Yano,S.
  TITLE     Molecular cloning and DNA sequence analysis of preproinsulin genes
            in the NON mouse, an animal model of human non-obese,
            non-insulin-dependent diabetes mellitus
  JOURNAL   J Mol Endocrinol 5 (1), 61-67 (1990)
   PUBMED   2397023
REFERENCE   9  (bases 1 to 611)
  AUTHORS   Wentworth,B.M., Schaefer,I.M., Villa-Komaroff,L. and Chirgwin,J.M.
  TITLE     Characterization of the two nonallelic genes encoding mouse
            preproinsulin
  JOURNAL   J Mol Evol 23 (4), 305-312 (1986)
   PUBMED   3104603
REFERENCE   10 (bases 1 to 611)
  AUTHORS   Bunzli,H.F., Glatthaar,B., Kunz,P., Mulhaupt,E. and Humbel,R.E.
  TITLE     Amino acid sequence of the two insulins from mouse (Maus musculus)
  JOURNAL   Hoppe Seylers Z Physiol Chem 353 (3), 451-458 (1972)
   PUBMED   5063718
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC163452.12, BP773571.1,
            AK008358.1 and BQ126282.1.
            
            On Aug 11, 2015 this sequence version replaced NM_008386.3.
            
            Summary: This gene encodes insulin, a peptide hormone that plays a
            vital role in the regulation of carbohydrate and lipid metabolism.
            The encoded precursor protein undergoes proteolytic cleavage to
            produce a disulfide-linked heterodimeric functional protein that is
            stored in secretory granules. An increase in blood glucose levels,
            among others, induces the release of insulin from the secretory
            granules. Mice deficient in the functional hormone encoded by this
            gene develop diabetes mellitus. [provided by RefSeq, Aug 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BP773571.1, BQ287625.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AC163452.12        161145-161167
            24-152              BP773571.1         499-627             c
            153-593             AK008358.1         2-442
            594-611             BQ126282.1         427-444
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 46.76 cM"
     gene            1..611
                     /gene="Ins1"
                     /gene_synonym="Ins-1; Ins2-rs1"
                     /note="insulin I"
                     /db_xref="GeneID:16333"
                     /db_xref="MGI:MGI:96572"
     exon            1..195
                     /gene="Ins1"
                     /gene_synonym="Ins-1; Ins2-rs1"
                     /inference="alignment:Splign:2.1.0"
     exon            196..601
                     /gene="Ins1"
                     /gene_synonym="Ins-1; Ins2-rs1"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..536
                     /gene="Ins1"
                     /gene_synonym="Ins-1; Ins2-rs1"
                     /note="insulin-1"
                     /codon_start=1
                     /product="insulin-1 preproprotein"
                     /protein_id="NP_032412.3"
                     /db_xref="CCDS:CCDS29898.1"
                     /db_xref="GeneID:16333"
                     /db_xref="MGI:MGI:96572"
                     /translation="MALLVHFLPLLALLALWEPKPTQAFVKQHLCGPHLVEALYLVCG
                     ERGFFYTPKSRREVEDPQVEQLELGGSPGDLQTLALEVARQKRGIVDQCCTSICSLYQ
                     LENYCN"
ORIGIN      
        1 accaggcaag tgtttggaaa ctgcagcttc agcccctctg gccatctgcc tacccacccc
       61 acctggagac cttaatgggc caaacagcaa agtccagggg gcagagagga ggtactttgg
      121 actataaagc tggtgggcat ccagtaaccc ccagccctta gtgaccagct ataatcagag
      181 accatcagca agcaggtcat tgtttcaaca tggccctgtt ggtgcacttc ctacccctgc
      241 tggccctgct tgccctctgg gagcccaaac ccacccaggc ttttgtcaaa cagcatcttt
      301 gtggtcccca cctggtagag gctctctacc tggtgtgtgg ggagcgtggc ttcttctaca
      361 cacccaagtc ccgccgtgaa gtggaggacc cacaagtgga acaactggag ctgggaggaa
      421 gccccgggga ccttcagacc ttggcgttgg aggtggcccg gcagaagcgt ggcattgtgg
      481 atcagtgctg caccagcatc tgctccctct accagctgga gaactactgc aactaaggcc
      541 cacctcgacc cgccccaccc ctctgcaatg aataaaactt ttgaataagc accaaaaaaa
      601 aaaaaaaaaa a
//
