LOCUS       NM_174876               6992 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus interphotoreceptor matrix proteoglycan 2 (Impg2),
            mRNA.
ACCESSION   NM_174876 XM_358856
VERSION     NM_174876.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Williams,B.N., Draper,A., Lang,P.F., Lewis,T.R., Smith,A.L.,
            Mayerl,S.J., Rougie,M., Simon,J.M., Arshavsky,V.Y., Greenwald,S.H.,
            Gamm,D.M., Pinilla,I. and Philpot,B.D.
  TITLE     Heterogeneity in the progression of retinal pathologies in mice
            harboring patient mimicking Impg2 mutations
  JOURNAL   Hum Mol Genet 33 (5), 448-464 (2024)
   PUBMED   37975905
  REMARK    GeneRIF: Heterogeneity in the progression of retinal pathologies in
            mice harboring patient mimicking Impg2 mutations.
            Erratum:[Hum Mol Genet. 2024 Feb 18;33(5):475-476. doi:
            10.1093/hmg/ddae004.. PMID: 38217426]
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Xu,R., Wang,Y., Du,J. and Salido,E.M.
  TITLE     Retinal Metabolic Profile on IMPG2 Deficiency Mice with Subretinal
            Lesions
  JOURNAL   Adv Exp Med Biol 1415, 457-463 (2023)
   PUBMED   37440072
  REMARK    GeneRIF: Retinal Metabolic Profile on IMPG2 Deficiency Mice with
            Subretinal Lesions.
REFERENCE   3  (bases 1 to 6992)
  AUTHORS   Mitchell,B., Coulter,C., Geldenhuys,W.J., Rhodes,S. and Salido,E.M.
  TITLE     Interphotoreceptor matrix proteoglycans IMPG1 and IMPG2 proteolyze
            in the SEA domain and reveal localization mutual dependency
  JOURNAL   Sci Rep 12 (1), 15535 (2022)
   PUBMED   36109576
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6992)
  AUTHORS   Zamfirescu,R.C., Day,M.L. and Morris,M.B.
  TITLE     mTORC1/2 signaling is downregulated by amino acid-free culture of
            mouse preimplantation embryos and is only partially restored by
            amino acid readdition
  JOURNAL   Am J Physiol Cell Physiol 320 (1), C30-C44 (2021)
   PUBMED   33052068
REFERENCE   5  (bases 1 to 6992)
  AUTHORS   Xu,H., Qu,C., Gan,L., Sun,K., Tan,J., Liu,X., Jiang,Z., Tian,W.,
            Liu,W., Zhang,S., Yang,Y., Jiang,L., Zhu,X. and Zhang,L.
  TITLE     Deletion of the Impg2 gene causes the degeneration of rod and cone
            cells in mice
  JOURNAL   Hum Mol Genet 29 (10), 1624-1634 (2020)
   PUBMED   32242237
  REMARK    GeneRIF: Deletion of the Impg2 gene causes the degeneration of rod
            and cone cells in mice.
REFERENCE   6  (bases 1 to 6992)
  AUTHORS   Salido,E.M. and Ramamurthy,V.
  TITLE     Proteoglycan IMPG2 Shapes the Interphotoreceptor Matrix and
            Modulates Vision
  JOURNAL   J Neurosci 40 (20), 4059-4072 (2020)
   PUBMED   32265257
  REMARK    GeneRIF: Proteoglycan IMPG2 Shapes the Interphotoreceptor Matrix
            and Modulates Vision.
            Erratum:[J Neurosci. 2020 Aug 19;40(34):6660. doi:
            10.1523/JNEUROSCI.1846-20.2020.. PMID: 32737169]
REFERENCE   7  (bases 1 to 6992)
  AUTHORS   Ghai,R., Bugarcic,A., Liu,H., Norwood,S.J., Skeldal,S.,
            Coulson,E.J., Li,S.S., Teasdale,R.D. and Collins,B.M.
  TITLE     Structural basis for endosomal trafficking of diverse transmembrane
            cargos by PX-FERM proteins
  JOURNAL   Proc Natl Acad Sci U S A 110 (8), E643-E652 (2013)
   PUBMED   23382219
REFERENCE   8  (bases 1 to 6992)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 6992)
  AUTHORS   Chen,Q., Cai,S., Shadrach,K.G., Prestwich,G.D. and Hollyfield,J.G.
  TITLE     Spacrcan binding to hyaluronan and other glycosaminoglycans.
            Molecular and biochemical studies
  JOURNAL   J Biol Chem 279 (22), 23142-23150 (2004)
   PUBMED   15044457
REFERENCE   10 (bases 1 to 6992)
  AUTHORS   Chen,Q., Lee,J.W., Nishiyama,K., Shadrach,K.G., Rayborn,M.E. and
            Hollyfield,J.G.
  TITLE     SPACRCAN in the interphotoreceptor matrix of the mouse retina:
            molecular, developmental and promoter analysis
  JOURNAL   Exp Eye Res 76 (1), 1-14 (2003)
   PUBMED   12589770
  REMARK    GeneRIF: May play an important role in photoreceptor development
            and maintenance.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY174061.1 and BC048863.1.
            
            On Apr 23, 2004 this sequence version replaced NM_174876.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC048863.1, SRR9219381.236170.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                AY174061.1         1-32
            33-135              BC048863.1         1-103
            136-1355            BC048863.1         105-1324
            1356-1360           AY174061.1         1356-1360
            1361-6992           BC048863.1         1330-6961
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 33.91 cM"
     gene            1..6992
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /note="interphotoreceptor matrix proteoglycan 2"
                     /db_xref="GeneID:224224"
                     /db_xref="MGI:MGI:3044955"
     exon            1..304
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..3951
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /note="sialoprotein associated with cones and rods
                     proteoglycan"
                     /codon_start=1
                     /product="interphotoreceptor matrix proteoglycan 2
                     precursor"
                     /protein_id="NP_777365.2"
                     /db_xref="CCDS:CCDS37358.1"
                     /db_xref="GeneID:224224"
                     /db_xref="MGI:MGI:3044955"
                     /translation="MIMFLPVGRMSLGILILFLTGGNLVSASEERQEPMHAVSVLSPE
                     KSTDLSLPTRKRQLLDATETGRRWLLRRRRSILFPNGVKICSSETVAEAVANHVKYFK
                     ARVCQEAIWEAFRTFWDRLPGRDEYRHWMNLCEDGVTSVFEMGAHFSQSVEHRNLIMK
                     KLAYTREAESSSCKDQSCGPELSFPVPIGETSTLTGAVSSASYPGLASESSAASPQES
                     ISNEIENVTEEPTQPAAEQIAEFSIQLLGKRYSEELRDPSSALYRLLVEEFISEVEKA
                     FTGLPGYKGIRVLEFRAPEENDSGIDVHYAVTFNGEAISNTTWDLISLHSNKVENHGL
                     VEMDDKPTAVYTISNFRDYIAETLHQNFLMGNSSLNPDPKSLQLINVRGVLLPQTEDI
                     VWNTQSSSLQVTTSSILDNTLQAEWLSADTTTTTTTTISPFGFSSSSPSATGRELQSQ
                     SALRDVVSTSKLASPTKVVLSSLPEILGGSSLTLHSVTPAVLQPDLPVAPEGRTSGSF
                     ILEDGLASTEELEDTSIDGLPSSPLIQPVPKETVPPMEDSDTALLSTPHLTSSAIEDL
                     TKDIGTPSGLESLASNISDQLEVIPWFPDTSVEKDFIFESGLGSGSGKDVDVIDWPWS
                     ETSLEKTTKPLSKSWSEEQDALLPTEGREKLHIDGRVDSTEQIIESSEHRYGDRPIHF
                     IEEESHVRSTIPIFVESATPPTSPIFSKHTSDVPDIDSYSLTKPPFLPVTIAIPASTK
                     KTDEVLKEDMVHTESSSHKELDSEVPVSRPDMQPVWTMLPESDTVWTRTSSLGKLSRD
                     TLASTPESTDRLWLKASMTQSTELPSTTHSTQLEEEVIMAVQDISLELDQVGTDYYQS
                     ELTEEQHGKADSYVEMSTSVHYTEMPIVALPTKGGVLSHTQTAGALVVFFSLRVTNML
                     FSEDLFNKNSLEYKALEQRFLELLVPYLQSNLSGFQNLEILSFRNGSIVVNSRVRFAE
                     SAPPNVNKAMYRILEDFCTTAYQTMNLDIDKYSLDVESGDEANPCKFQACNEFSECLV
                     NPWSGEAKCKCYPGYLSVDELPCQSLCDLQPDFCLNDGKCDIMPGHGAICRCRVGSNW
                     WYRGQHCEEFVSEPFVIGITIASVVSFLLVASAVVFFLVKMLQAQNVRRERQRPTSSS
                     RHPDSLSSVENAMKYNPAYESHLAGCELYEKSYSQHPFYSSASEEVIGGLSREEIRQM
                     YESSDLSKEEIQERMRILELYANDPEFAAFVREHQMEEL"
     exon            305..532
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            533..699
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            700..731
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            732..755
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            756..805
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            806..873
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1035
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1094
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1115
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1360
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1446
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1756
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..3030
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            3031..3250
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            3251..3461
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            3462..3650
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3858
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            3859..3938
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     exon            3939..6912
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4178..4183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /note="hexamer: ATTAAA"
     polyA_site      4178
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /note="major polyA site"
     polyA_site      4199
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /note="major polyA site"
     regulatory      4227..4232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
                     /note="hexamer: AATAAA"
     polyA_site      4249
                     /gene="Impg2"
                     /gene_synonym="IPM200; PG10.2; Rsbp; Spacrcan"
ORIGIN      
        1 atagtgtccc tattaagtca gaaaagaaac cagagcttct cggaaaggat gacattttat
       61 ttacgctaac tgacaatggt tttagagtgg cttcccactc ccaggcataa acttttatct
      121 gcagggactt tttttgggaa aagtttgtta gggagcattt tagaactccg aatgctccct
      181 tgaggatttt gtcatctgtt ttctttggtt ttggcccaga tgattatgtt tctccctgtg
      241 gggaggatgt ctctgggcat tttgatactt ttcctgacag gaggaaacct tgtatcagcc
      301 tcagaagaga gacaagaacc catgcatgcc gtctctgtcc tctcacctga gaagtcaaca
      361 gacctttccc tgcctaccag gaagaggcag cttttggatg ccacggagac tgggaggagg
      421 tggctgctta gaaggcggcg atctattctg ttcccaaatg gagtaaaaat ttgctccagt
      481 gaaaccgttg cagaggctgt ggcaaaccat gtgaagtatt ttaaagcccg agtgtgccag
      541 gaagccatct gggaagcctt caggacgttt tgggatcgac ttcctgggcg tgatgaatat
      601 cgtcactgga tgaatttatg tgaggatgga gtcacaagtg tatttgaaat gggcgcccat
      661 tttagtcagt ctgtggaaca tagaaaccta atcatgaaga aactggctta cacaagggaa
      721 gctgagagca gctcctgcaa ggatcagtcc tgtgggcctg agttgtcctt tccagttcct
      781 attggtgaga cctcaacact gacaggtgct gtctccagtg cttcctatcc agggttggct
      841 tcggagagca gcgcagcgtc accgcaggag agtatcagca atgaaattga gaatgtgaca
      901 gaggagccca cacaaccagc tgctgaacag attgcggaat tcagcatcca acttctgggg
      961 aagcgataca gtgaagaact gcgggatccc tccagcgccc tctaccggct cctcgtggaa
     1021 gagtttattt cagaggttga aaaagcattc acagggttac ctggctacaa gggcatccgt
     1081 gttctggaat tcagggcccc ggaggaaaat gacagtggga tagatgttca ctatgcagtt
     1141 accttcaatg gcgaagccat cagcaatacc acctgggacc tcataagcct tcactccaac
     1201 aaggtagaaa accatggcct tgtagagatg gatgataaac ccactgctgt ctatacaatt
     1261 agtaacttca gagattatat cgctgagacg ctgcaccaga actttttgat gggaaattcc
     1321 tctttgaatc cagatcccaa gtctctccag ctcatcaatg tgagaggagt tttgctcccc
     1381 caaacagaag acatagtttg gaacacccaa agttcaagtc ttcaggtgac aacatcctct
     1441 attttggata atacccttca agctgaatgg ctctcagcag ataccaccac caccaccacc
     1501 accaccattt caccatttgg tttcagctcc agttctccct cagccactgg cagggaactc
     1561 cagtcacaaa gtgctttgcg tgacgtagtg tctacctcca agttagcttc tcccacgaag
     1621 gtggtcctca gttctctccc agagatttta gggggtagca gcttgactct tcattctgtt
     1681 accccagcag tgcttcagcc tgacctgcct gtggctcctg agggaaggac ttctggatcg
     1741 ttcatattag aagatgggtt agccagcact gaagaattag aagatacttc tattgatgga
     1801 ttgccttcaa gcccattaat tcaacctgtg ccaaaagaaa cagtaccacc tatggaagac
     1861 tctgacacgg ctctcttgtc cacaccacat ctgacctctt ctgctataga agaccttact
     1921 aaagacatag ggacaccttc tggcttggag tccttggctt ccaacatctc agaccagttg
     1981 gaagtgatcc catggtttcc agacacctct gtggaaaaag acttcatttt tgaaagtggc
     2041 ttgggttctg ggtctgggaa agatgtagat gtgattgatt ggccatggag tgagacttca
     2101 ttagagaaga ccactaaacc actgtcaaag tcatggtctg aagaacagga tgcactatta
     2161 ccaactgagg gtagagaaaa attacatata gatggcagag tagattccac agaacaaatt
     2221 attgaatcat cagaacatag atatggagat aggcccatac attttataga ggaagaatcc
     2281 catgttagat ctactatacc catctttgta gagtccgcaa ctccacctac atctccaatc
     2341 ttttcaaaac acacttcaga tgtaccagac attgattctt actcacttac caaaccaccc
     2401 ttcttaccgg taactatagc aatccctgct tccactaaga aaacagatga ggtactcaag
     2461 gaagatatgg tacatacaga atcatccagt cacaaagaac ttgacagtga ggttccagtg
     2521 tcaaggccag atatgcagcc tgtgtggacc atgttgccag aatcagatac agtttggaca
     2581 agaacttctt ccttagggaa attgtccaga gacacattgg caagtacacc agagagcact
     2641 gacagactct ggttgaaagc ttccatgaca cagtccactg aattgccttc aaccacccac
     2701 tccacccagc tagaggagga agtaataatg gcggtccagg atatttcatt agaactagat
     2761 caggtaggca cagattatta tcagtccgag ctaactgaag aacaacatgg caaggctgac
     2821 agctatgtgg aaatgtctac cagtgttcac tacacagaga tgcctattgt ggctctgccc
     2881 acaaaaggag gtgtcttgag tcacacccag actgcaggag cattggtggt tttcttcagc
     2941 ctccgcgtga caaacatgtt gttttcagaa gacttgttta acaaaaactc tttggaatat
     3001 aaagccctgg aacaaagatt cttagaactg ctggttccct atctccagtc aaatctgtca
     3061 gggttccaga acctagaaat cctgagtttc agaaacggca gcattgtggt gaacagccga
     3121 gtgaggttcg ccgagtctgc ccctcctaat gtcaacaagg ccatgtatag gattctggaa
     3181 gacttttgta ccactgccta ccaaaccatg aacttggata tcgataagta ctccctggac
     3241 gtggaatcag gtgatgaggc caacccttgc aagtttcagg cctgtaatga attttctgag
     3301 tgtttggtaa atccatggag tggagaagca aagtgcaaat gctaccctgg gtacctgagt
     3361 gtggatgaac tgccttgtca aagtctctgt gatctacagc ctgacttctg cttgaacgat
     3421 ggaaagtgtg acattatgcc tgggcatgga gccatttgta gatgccgggt tggttcaaac
     3481 tggtggtatc gaggccaaca ctgtgaggag tttgtgtctg agccctttgt cataggcatc
     3541 actatagcct ctgtggttag ctttctcctt gttgcttctg ctgtcgtctt cttccttgtg
     3601 aagatgcttc aagctcagaa tgtcaggaga gaaaggcaga ggcccaccag ctccagcagg
     3661 caccctgaca gtctgtcatc tgttgagaat gctatgaagt ataaccctgc atatgagagc
     3721 cacttggctg gatgtgaact gtatgagaaa tcctatagcc aacatccctt ctatagctct
     3781 gctagtgaag aggtgattgg tggtctgagc agagaagaaa tcagacagat gtatgaaagt
     3841 agcgaccttt ccaaagagga aattcaagag agaatgagga ttttggaact ctatgctaat
     3901 gatcctgagt ttgcagcttt tgtgagagag catcaaatgg aggagcttta acttaaatgc
     3961 ctgattcttg acaccaatca gaagcttgga ggagatggag aaggcttgtt ctctctgctg
     4021 tttaactaat ccagaagaag agtttgtatt gaagaataaa taaggaaaca tgggacgcac
     4081 ttctcattcc aacactgcag cttaattttt tggaatggag caaaaaaaaa ataagtgatg
     4141 tattttattt ctacattaag agatgtgtca aaagaaaatt aaagtggtgt gaactctgat
     4201 tttgtaacat attctaaaag caaacaaata aaacagaacc aaaccaaaag cttaaagcca
     4261 gaccttggag ttggggctgc agtgcctctg actctgactt tttgagagca tctctaagaa
     4321 ctatggccca ggctttctag taagaacata aagtgagact aatgagtaaa gcttagaatg
     4381 cgactgtttt gtgacatact tgttaaagtc gaatgagata gaggaagctt tgaagtaatt
     4441 ttaatatagt ttaaactcaa acactcatct aaataaaaat taggcttttg gaacagattg
     4501 ctgagtcagg caatctttag gtgcagtata ttttgtttat gtttgatgct tgcttctatc
     4561 tgttcttgag cttcttgagc ccatagatca agactacaat gctcttaaat tagttatgtc
     4621 aatatttgcc acagtttggt cctcaattag gcacccttaa gaggaagcaa attgaggaat
     4681 tattttcatc agcttggctt gtggacatac cagtgggcct ttttcttgat tattaattga
     4741 tgtagaaagg cccagctcac tatgggtggt actatcctta ggcaggggtt tggggagtta
     4801 agttgcaaaa gaaaggtaaa gccagctaca agaagccagc caataagcac tttcctttgt
     4861 ggtttcttct tcaaactcct gtcttggctt ctctctatgg tagactataa cctataagcc
     4921 aaataaactc tttctttctg tcctagttag ggtttccatt gttgtgacca aggcaactct
     4981 tttaaggaca acatttaatc gaggctggct tgtagattga ggttcagtcc attatcatca
     5041 aagctggaag catggcagca tccaggcagt caaggcactg gaggagctga gagttcttca
     5101 tcttcatcta aaggaagcca ggagcagact agctttcagg cagctaggat gagggtctta
     5161 aaacacatgc ccacagtgac acacctactc taacaaggcc acacctactc caacaaggcc
     5221 atactttcaa atagtgccac tccctgggcc aaacatattt acaccaccat accttctcaa
     5281 aggtgctttt gcttagtgat aacagagaaa gcaaatgatt ataataccct attctttaag
     5341 ctttcaggaa tagtacccat gcgtgaagtt ttcatggttt ctatttttct tatgcaaaag
     5401 taattatggg gctgtaaggg tctgagtgtt aggtaaatac caggacagac cctctggttt
     5461 ttagatgggc tgacaggcaa agttattact tagatatgtt ccctgactgc ttctgaggta
     5521 ggacagtgta gtggacaatt ctaggaccta ctgtagttcc tggtgtagga attttgaaaa
     5581 cagcacatac ctacctcaca taatgtcagt gatatcattt gttgcttctt tgtctaaagc
     5641 ttgttgtcta ggtgaaatcc cacctaccat aacaggacat ttttaaaatt gtgaatctat
     5701 actttataaa tagaagttaa aaatggaagt ttttctcttt ttacctatgg ttattttgaa
     5761 tggataattt gggtgatagg ctgtaaaggc gaattaatca tgatgaatag tttaaagata
     5821 cataggcact agatgattgt taattcaagc tatttctgag gacaccttca tgtttgcaac
     5881 aaatctcttg aagttcatta gttatttcag accaagtata aaatggcagc aggataaaat
     5941 acctggttca tttatttttc ttaaatcagg gactgtaagt ttcatagtta tgaggtcagc
     6001 aaaaactgga gttggaagaa tagacaactt acttagaaga aaacagtgat ttaggagact
     6061 aacttacatt tattaaattg ggaagtttta gccaagaaaa atgtgggtca gtttcccccc
     6121 accctctcaa atgtttccaa tgattccaag taaagatagg tggggccagc cttcattagt
     6181 aacccagctg cagtgaatca ggattctcga tcattctatt ccctagtgtg tgatcaatga
     6241 agaaaaatga tgtaaaggtt acagtcctta aactgagagc attgtgtacg caaagtaccc
     6301 tgcccagagg tggggagatg ggaggatatt ataacatagt tggtgatgta aaaaaaaaat
     6361 tacctggaaa tttctttgat tcatgcattt tgtaatgtgt agactgttct aataagtcaa
     6421 caagagacaa gtgaagccac tccccactgg atactaagcc actgactaaa acacgtctct
     6481 caactacata gttactatta cagctcaggc caaatgatta gtaatcctga ttttttctct
     6541 tgtatttaat taattaaggg cttaattggg ggaaaaatca tggtataaga gactacatgt
     6601 aaaaaaaaag agactacatg tataaatata tgtctggact acttgatgat tcctaagcag
     6661 taatgtatat atcctaatat atacttaata tggagtatgg ttaatggcat gctattcttt
     6721 tttatttaat ttatggcagt ttatccaatg tggggtcaaa caaagtcaat ttactatact
     6781 actctcttga acatttctag tcataatatt agttgattag aaatacaaat ttaagtcttt
     6841 atgtatgtat aaggatcctt cgattcacat gcatactgtg atgtatccat tagtaataaa
     6901 catgtctttc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     6961 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//
