LOCUS       NM_021782               3090 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus interleukin 21 (Il21), transcript variant 2, mRNA.
ACCESSION   NM_021782 XM_001479075 XM_622579
VERSION     NM_021782.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Li,X., Sun,W., Huang,M., Gong,L., Zhang,X., Zhong,L., Calderon,V.,
            Bian,Z., He,Y., Suh,W.K., Li,Y., Song,T., Zou,Y., Lian,Z.X. and
            Gu,H.
  TITLE     Deficiency of CBL and CBLB ubiquitin ligases leads to hyper T
            follicular helper cell responses and lupus by reducing BCL6
            degradation
  JOURNAL   Immunity 57 (7), 1603-1617 (2024)
   PUBMED   38761804
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Seyedsadr,M., Bang,M.F., McCarthy,E.C., Zhang,S., Chen,H.C.,
            Mohebbi,M., Hugo,W., Whitmire,J.K., Lechner,M.G. and Su,M.A.
  TITLE     A pathologically expanded, clonal lineage of IL-21-producing CD4+ T
            cells drives inflammatory neuropathy
  JOURNAL   J Clin Invest 134 (15), e178602 (2024)
   PUBMED   39087473
  REMARK    GeneRIF: A pathologically expanded, clonal lineage of
            IL-21-producing CD4+ T cells drives inflammatory neuropathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3090)
  AUTHORS   Johnson,J.T., Surette,F.A., Ausdal,G.R., Shah,M., Minns,A.M.,
            Lindner,S.E., Zander,R.A. and Butler,N.S.
  TITLE     CD4 T Cell-Derived IL-21 Is Critical for Sustaining Plasmodium
            Infection-Induced Germinal Center Responses and Promoting the
            Selection of Memory B Cells with Recall Potential
  JOURNAL   J Immunol 212 (9), 1467-1478 (2024)
   PUBMED   38477614
  REMARK    GeneRIF: CD4 T Cell-Derived IL-21 Is Critical for Sustaining
            Plasmodium Infection-Induced Germinal Center Responses and
            Promoting the Selection of Memory B Cells with Recall Potential.
REFERENCE   4  (bases 1 to 3090)
  AUTHORS   Chan,C.W., Chen,H.W., Wang,Y.W., Lin,C.I. and Chuang,Y.H.
  TITLE     IL-21, not IL-17A, exacerbates murine primary biliary cholangitis
  JOURNAL   Clin Exp Immunol 215 (2), 137-147 (2024)
   PUBMED   37708215
  REMARK    GeneRIF: IL-21, not IL-17A, exacerbates murine primary biliary
            cholangitis.
REFERENCE   5  (bases 1 to 3090)
  AUTHORS   Tang,L., Li,Q., Chen,L., Li,X., Gu,S., He,W., Pan,Q., Wang,L.,
            Sun,J., Yi,X. and Li,Y.
  TITLE     IL-21 collaborates with anti-TIGIT to restore NK cell function in
            chronic HBV infection
  JOURNAL   J Med Virol 95 (10), e29142 (2023)
   PUBMED   37815034
  REMARK    GeneRIF: IL-21 collaborates with anti-TIGIT to restore NK cell
            function in chronic HBV infection.
REFERENCE   6  (bases 1 to 3090)
  AUTHORS   Suto,A., Nakajima,H., Hirose,K., Suzuki,K., Kagami,S., Seto,Y.,
            Hoshimoto,A., Saito,Y., Foster,D.C. and Iwamoto,I.
  TITLE     Interleukin 21 prevents antigen-induced IgE production by
            inhibiting germ line C(epsilon) transcription of IL-4-stimulated B
            cells
  JOURNAL   Blood 100 (13), 4565-4573 (2002)
   PUBMED   12393685
  REMARK    GeneRIF: IL-21 down-regulates IgE production from IL-4-stimulated B
            cells through the inhibition of germ line C(epsilon) transcription
            and thus suggest that IL-21 may be useful for the treatment of
            IgE-dependent allergic diseases.
REFERENCE   7  (bases 1 to 3090)
  AUTHORS   Ozaki,K., Spolski,R., Feng,C.G., Qi,C.F., Cheng,J., Sher,A.,
            Morse,H.C. 3rd, Liu,C., Schwartzberg,P.L. and Leonard,W.J.
  TITLE     A critical role for IL-21 in regulating immunoglobulin production
  JOURNAL   Science 298 (5598), 1630-1634 (2002)
   PUBMED   12446913
  REMARK    GeneRIF: has a significant influence on the regulation of B cell
            function in vivo and cooperates with IL-4
REFERENCE   8  (bases 1 to 3090)
  AUTHORS   Wurster,A.L., Rodgers,V.L., Satoskar,A.R., Whitters,M.J.,
            Young,D.A., Collins,M. and Grusby,M.J.
  TITLE     Interleukin 21 is a T helper (Th) cell 2 cytokine that specifically
            inhibits the differentiation of naive Th cells into interferon
            gamma-producing Th1 cells
  JOURNAL   J Exp Med 196 (7), 969-977 (2002)
   PUBMED   12370258
  REMARK    GeneRIF: a T helper (Th) cell 2 cytokine that specifically inhibits
            the differentiation of naive Th cells into interferon
            gamma-producing Th1 cells
REFERENCE   9  (bases 1 to 3090)
  AUTHORS   Kasaian,M.T., Whitters,M.J., Carter,L.L., Lowe,L.D., Jussif,J.M.,
            Deng,B., Johnson,K.A., Witek,J.S., Senices,M., Konz,R.F.,
            Wurster,A.L., Donaldson,D.D., Collins,M., Young,D.A. and
            Grusby,M.J.
  TITLE     IL-21 limits NK cell responses and promotes antigen-specific T cell
            activation: a mediator of the transition from innate to adaptive
            immunity
  JOURNAL   Immunity 16 (4), 559-569 (2002)
   PUBMED   11970879
  REMARK    GeneRIF: IL-21 promotes the transition between innate and adaptive
            immunity.
REFERENCE   10 (bases 1 to 3090)
  AUTHORS   Parrish-Novak,J., Dillon,S.R., Nelson,A., Hammond,A., Sprecher,C.,
            Gross,J.A., Johnston,J., Madden,K., Xu,W., West,J., Schrader,S.,
            Burkhead,S., Heipel,M., Brandt,C., Kuijper,J.L., Kramer,J.,
            Conklin,D., Presnell,S.R., Berry,J., Shiota,F., Bort,S., Hambly,K.,
            Mudri,S., Clegg,C., Moore,M., Grant,F.J., Lofton-Day,C.,
            Gilbert,T., Rayond,F., Ching,A., Yao,L., Smith,D., Webster,P.,
            Whitmore,T., Maurer,M., Kaushansky,K., Holly,R.D. and Foster,D.
  TITLE     Interleukin 21 and its receptor are involved in NK cell expansion
            and regulation of lymphocyte function
  JOURNAL   Nature 408 (6808), 57-63 (2000)
   PUBMED   11081504
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL662823.12.
            
            On Mar 22, 2014 this sequence version replaced NM_021782.2.
            
            Transcript Variant: This variant (2) lacks a segment in the 3'
            coding region which results in a frameshift compared to variant 1.
            The encoded isoform (2) is shorter and has a distinct C-terminus
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF254070.1, BC125416.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849380,
                                           SAMN00849383 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-218               AL662823.12        203786-204003       c
            219-254             AL662823.12        203634-203669       c
            255-410             AL662823.12        199022-199177       c
            411-488             AL662823.12        196816-196893       c
            489-3090            AL662823.12        194126-196727       c
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 18.36 cM"
     gene            1..3090
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /note="interleukin 21"
                     /db_xref="GeneID:60505"
                     /db_xref="MGI:MGI:1890474"
     exon            1..218
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..512
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="interleukin-21 isoform 2 precursor"
                     /protein_id="NP_068554.1"
                     /db_xref="CCDS:CCDS17317.1"
                     /db_xref="GeneID:60505"
                     /db_xref="MGI:MGI:1890474"
                     /translation="MERTLVCLVVIFLGTVAHKSSPQGPDRLLIRLRHLIDIVEQLKI
                     YENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPA
                     RRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS"
     exon            219..254
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /inference="alignment:Splign:2.1.0"
     exon            255..410
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /inference="alignment:Splign:2.1.0"
     exon            411..488
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /inference="alignment:Splign:2.1.0"
     exon            489..3090
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      958..963
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /note="hexamer: AATAAG"
     polyA_site      982
                     /gene="Il21"
                     /gene_synonym="IL-21"
                     /note="major polyA site"
ORIGIN      
        1 gcacagtcag gttgaagtga gaaccagacc aaggccctgt catcagctcc tggagactca
       61 gttctggtgg catggagagg acccttgtct gtctggtagt catcttcttg gggacagtgg
      121 cccataaatc aagcccccaa gggccagatc gcctcctgat tagacttcgt caccttattg
      181 acattgttga acagctgaaa atctatgaaa atgacttgga tcctgaactt ctatcagctc
      241 cacaagatgt aaaggggcac tgtgagcatg cagcttttgc ctgttttcag aaggccaaac
      301 tcaagccatc aaaccctgga aacaataaga cattcatcat tgacctcgtg gcccagctca
      361 ggaggaggct gcctgccagg aggggaggaa agaaacagaa gcacatagct aaatgccctt
      421 cctgtgattc gtatgagaaa aggacaccca aagaattcct agaaagacta aaatggctcc
      481 ttcaaaagat gattcatcag catctctcct agaacacata ggacccgaag attcctgagg
      541 atccgagaag attcccgagg actgaggaga cgccggacac tatagacgct cacgaatgca
      601 ggagtacatc ttgcctcttg ggattgcaag tggagaagta cgatacgtta tgataagaac
      661 aactcagaaa agctataggt taagatcctt tcgcccatta actaagcaga cattgtggtt
      721 ccctgcacag actccatgct gtcaacatgg aaaatctcaa ctcaacaaga gcccagcttc
      781 ccgtgtcagg gatttctggt gcttctcaag ctgtggcttc atcttattgc ccaactgtga
      841 cattctttga ttggaagggg aaaactaaag cttttagcaa aaatacagct agggaatttg
      901 tcgatctgcg agagtaagac ctcttatgat cctaacggaa tgatgtaagc tggaaataat
      961 aagcataaga tgaaattgaa aattgaagtc tttattcttt aagaaaaact ttgtacttga
     1021 aagcatgtct gaagagttta ctcattacca caaacatcta gcatattgat aactaacatc
     1081 tttatactct acaagagagg ctttccagat aggtacagtt tttcttctct attaggtcta
     1141 tcaaaattta acctattatg agggtcaccc ctggctttca ctgtttttct aaagaggcaa
     1201 gggtgtagta agaagcaggc ttaagttgcc ttcctcccaa tgtcaagttc ctttataagc
     1261 taatagttta atcttgtgaa gatggcaatg aaagcctgtg gaagtgcaaa cctcactatc
     1321 ttctggagcc aagtagaatt ttcaagtttg tagctctcac ctcaagtggt tatgggtgtc
     1381 ctgtgatgaa tctgctagct ccagcctcag tctcctctcc cacatccttt cctttctttc
     1441 ctctttgaaa cttctaagaa aaagcaatcc aaacaagttc agcacttaag acacattgca
     1501 tgcacacttt tgataagtta aatccaacca tctatttaaa atcaaaatca ggagatgagc
     1561 caagagacca gaggttctgt tccagtttta aacagacttt tactgaacat cccaatcttt
     1621 taaccacaga ggctaaattg agcaaatagt tttgccattt gatataattt ccaacagtat
     1681 gtttcaatgt caagttaaaa agtctacaaa gctattttcc ctggagtggt atcatcgctt
     1741 tgagaatttc ttatggttaa aatggatctg agatccaagc atggcctggg ggatggtttt
     1801 gatctaagga aaaaggtgtc tgtacctcac agtgccttta aaacaagcag agatcccgtg
     1861 taccgcccta agatagcaca gactagtgtt aactgattcc cagaaaagtg tcacaatcag
     1921 aaccaacgca ttctcttaaa ctttaaaaat atgtattgca aagaacttgt gtaactgtaa
     1981 atgtgtgact gttgatgaca ttatacacac atagcccacg taagtgtcca atggtgctag
     2041 cattggttgc tgagtttgct gctcgaaagc tgaagcagag atgcagtcct tcacaaagca
     2101 atgatggaca gagaggggag tctccatgtt ttattctttt gttgtttctg gctgtgtaac
     2161 tgttgacttc ttgacattgt gatttttata tttaagacaa tgtatttatt ttggtgtgtt
     2221 tattgttcta gccttttaaa tcactgacaa tttctaatca agaagtacaa ataattcaat
     2281 gcagcacagg ctaagagctt gtatcgtttg gaaaagccag tgaaggcttc tccactagcc
     2341 atgggaaagc tacgctttag agtaaactag acaaaattgc acagcagtct tgaacctctc
     2401 tgtgctcaag actcagccag tcctttgaca ttattgttca ctgtgggtgg gaacacattg
     2461 gacctgacac actgttgtgt gtccatgaag gttgccactg gtgtaagctt tttttggttt
     2521 tcattctctt atctgtagaa caagaatgtg gggctttcct aagtctattc tgtattttat
     2581 tctgaacttc gtatgtctga gttttaatgt tttgagtact cttacaggaa cacctgacca
     2641 cacttttgag ttaaatttta tcccaagtgt gatatttagt tgttcaaaaa gggaagggat
     2701 atacatacat acatacatac atacatacat atatatatat atatatacat atatatatat
     2761 atatatatgt atatatatat atatatagag agagagagag agagagagag agaaagagag
     2821 agaggttgtt gtaggtcata ggagttcaga ggaaatcagt tatggccgtt aatactgtag
     2881 ctgaaagtgt tttctttgtg aataaattca tagcattatt gatctatgtt attgctctgt
     2941 tttatttaca gtcacacctg agaatttagt tttaatatga atgatgtact ttataactta
     3001 atgattattt attatgtatt tggttttgaa tgtttgtgtt catggcttct tatttaagac
     3061 ctgatcatat taaatgctac ccagtcagaa
//
