LOCUS       NM_008351               1345 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus interleukin 12a (Il12a), transcript variant 2, mRNA.
ACCESSION   NM_008351 XM_980710
VERSION     NM_008351.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Mittereder,L.R., Swoboda,J., De Pascalis,R. and Elkins,K.L.
  TITLE     IL-12p40 is essential but not sufficient for Francisella tularensis
            LVS clearance in chronically infected mice
  JOURNAL   PLoS One 18 (3), e0283161 (2023)
   PUBMED   36972230
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1345)
  AUTHORS   Ahmadi,F., Junghus,F., Ashworth,C., Lappalainen,A., Morbe,U.,
            Kotarsky,K. and Agace,W.W.
  TITLE     cDC1-derived IL-27 regulates small intestinal CD4+ T cell
            homeostasis in mice
  JOURNAL   J Exp Med 220 (3) (2023)
   PUBMED   36515659
REFERENCE   4  (bases 1 to 1345)
  AUTHORS   Bhattarai,U., He,X., Xu,R., Liu,X., Pan,L., Sun,Y., Chen,J.X. and
            Chen,Y.
  TITLE     IL-12alpha deficiency attenuates pressure overload-induced cardiac
            inflammation, hypertrophy, dysfunction, and heart failure
            progression
  JOURNAL   Front Immunol 14, 1105664 (2023)
   PUBMED   36860846
  REMARK    GeneRIF: IL-12alpha deficiency attenuates pressure overload-induced
            cardiac inflammation, hypertrophy, dysfunction, and heart failure
            progression.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1345)
  AUTHORS   Li,S., Mirlekar,B., Johnson,B.M., Brickey,W.J., Wrobel,J.A.,
            Yang,N., Song,D., Entwistle,S., Tan,X., Deng,M., Cui,Y., Li,W.,
            Vincent,B.G., Gale,M. Jr., Pylayeva-Gupta,Y. and Ting,J.P.
  TITLE     STING-induced regulatory B cells compromise NK function in cancer
            immunity
  JOURNAL   Nature 610 (7931), 373-380 (2022)
   PUBMED   36198789
REFERENCE   6  (bases 1 to 1345)
  AUTHORS   Murphy,T.L., Cleveland,M.G., Kulesza,P., Magram,J. and Murphy,K.M.
  TITLE     Regulation of interleukin 12 p40 expression through an NF-kappa B
            half-site
  JOURNAL   Mol Cell Biol 15 (10), 5258-5267 (1995)
   PUBMED   7565674
REFERENCE   7  (bases 1 to 1345)
  AUTHORS   Heinzel,F.P., Rerko,R.M., Ahmed,F. and Pearlman,E.
  TITLE     Endogenous IL-12 is required for control of Th2 cytokine responses
            capable of exacerbating leishmaniasis in normally resistant mice
  JOURNAL   J Immunol 155 (2), 730-739 (1995)
   PUBMED   7608551
REFERENCE   8  (bases 1 to 1345)
  AUTHORS   Liu,W. and Kurlander,R.J.
  TITLE     Analysis of the interrelationship between IL-12, TNF-alpha, and
            IFN-gamma production during murine listeriosis
  JOURNAL   Cell Immunol 163 (2), 260-267 (1995)
   PUBMED   7606797
REFERENCE   9  (bases 1 to 1345)
  AUTHORS   Smith,T.J., Ducharme,L.A. and Weis,J.H.
  TITLE     Preferential expression of interleukin-12 or interleukin-4 by
            murine bone marrow mast cells derived in mast cell growth factor or
            interleukin-3
  JOURNAL   Eur J Immunol 24 (4), 822-826 (1994)
   PUBMED   7512032
REFERENCE   10 (bases 1 to 1345)
  AUTHORS   Schoenhaut,D.S., Chua,A.O., Wolitzky,A.G., Quinn,P.M., Dwyer,C.M.,
            McComas,W., Familletti,P.C., Gately,M.K. and Gubler,U.
  TITLE     Cloning and expression of murine IL-12
  JOURNAL   J Immunol 148 (11), 3433-3440 (1992)
   PUBMED   1350290
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121306.12.
            
            On Aug 8, 2022 this sequence version replaced NM_008351.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M86672.1, BC116855.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-215               AC121306.12        37644-37858         c
            216-349             AC121306.12        37054-37187         c
            350-463             AC121306.12        34936-35049         c
            464-505             AC121306.12        33979-34020         c
            506-547             AC121306.12        33864-33905         c
            548-691             AC121306.12        33630-33773         c
            692-1345            AC121306.12        30662-31315         c
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 31.92 cM"
     gene            1..1345
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="interleukin 12a"
                     /db_xref="GeneID:16159"
                     /db_xref="MGI:MGI:96539"
     exon            1..215
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..847
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; interleukin-12 subunit alpha; interleukin 12a
                     p35 subunit; CLMF p35; IL-12 subunit p35; cytotoxic
                     lymphocyte maturation factor 35 kDa subunit; IL-12 p35
                     subunit; interleukin-12 p35 subunit"
                     /codon_start=1
                     /product="interleukin-12 subunit alpha isoform 2
                     precursor"
                     /protein_id="NP_032377.1"
                     /db_xref="CCDS:CCDS17400.1"
                     /db_xref="GeneID:16159"
                     /db_xref="MGI:MGI:96539"
                     /translation="MCQSRYLLFLATLALLNHLSLARVIPVSGPARCLSQSRNLLKTT
                     DDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTT
                     RGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAID
                     ELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA"
     exon            216..349
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            350..463
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            464..505
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            506..547
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            548..691
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            692..1345
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1323..1328
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="hexamer: AATAAA"
     polyA_site      1345
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="major polyA site"
ORIGIN      
        1 aattcgaaag cgccaccagc cttgggggcg atcgacgcac ttgtccttga gatgtagaga
       61 cagagagaga aagcaagaga cacagtcctg ggaaagtcct gccggctatc cagacaatta
      121 taaaaatgtg tctcccaagg tcagcgttcc aacagcctca ccctcggcat ccagcagctc
      181 ctctcagtgc cggtccagca tgtgtcaatc acgctacctc ctctttttgg ccacccttgc
      241 cctcctaaac cacctcagtt tggccagggt cattccagtc tctggacctg ccaggtgtct
      301 tagccagtcc cgaaacctgc tgaagaccac agatgacatg gtgaagacgg ccagagaaaa
      361 actgaaacat tattcctgca ctgctgaaga catcgatcat gaagacatca cacgggacca
      421 aaccagcaca ttgaagacct gtttaccact ggaactacac aagaacgaga gttgcctggc
      481 tactagagag acttcttcca caacaagagg gagctgcctg cccccacaga agacgtcttt
      541 gatgatgacc ctgtgccttg gtagcatcta tgaggacttg aagatgtacc agacagagtt
      601 ccaggccatc aacgcagcac ttcagaatca caaccatcag cagatcattc tagacaaggg
      661 catgctggtg gccatcgatg agctgatgca gtctctgaat cataatggcg agactctgcg
      721 ccagaaacct cctgtgggag aagcagaccc ttacagagtg aaaatgaagc tctgcatcct
      781 gcttcacgcc ttcagcaccc gcgtcgtgac catcaacagg gtgatgggct atctgagctc
      841 cgcctgaaag gctcaaggcc ctctgccaca gcgccctcct cacacagata ggaaacaaag
      901 aaagattcat aagagtcagg tggtcttggc ctggtgggcc ttaagctcct tcaggaatct
      961 gttctcccat cacatctcat ctccccaaag gtggcacagc tacctcagca tggtcccctc
     1021 catcgcttct ctcatattca ctatacaagt tgtttgtaag ttttcatcaa aatattgtta
     1081 aggggcgaag acgtcctccc ctcaatgtgt tagcagaaga gcaagaactg ataagctatt
     1141 gtttttgtgc caaagtgttt atgaaaacac tcagtcaccc cttatttaaa aatatttatt
     1201 gctatatttt atactcatga aagtacatga gcctatttat atttatttat tttctattta
     1261 ttataatatt tcttatcaga tgaatttgaa acattttgaa acatacctta ttttgtggtt
     1321 ctaataaagt aatgttatca cttca
//
