LOCUS       NM_001410419            1118 bp    mRNA    linear   ROD 21-AUG-2024
DEFINITION  Mus musculus interleukin 12a (Il12a), transcript variant 5, mRNA.
ACCESSION   NM_001410419
VERSION     NM_001410419.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Mittereder,L.R., Swoboda,J., De Pascalis,R. and Elkins,K.L.
  TITLE     IL-12p40 is essential but not sufficient for Francisella tularensis
            LVS clearance in chronically infected mice
  JOURNAL   PLoS One 18 (3), e0283161 (2023)
   PUBMED   36972230
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1118)
  AUTHORS   Ahmadi,F., Junghus,F., Ashworth,C., Lappalainen,A., Morbe,U.,
            Kotarsky,K. and Agace,W.W.
  TITLE     cDC1-derived IL-27 regulates small intestinal CD4+ T cell
            homeostasis in mice
  JOURNAL   J Exp Med 220 (3) (2023)
   PUBMED   36515659
REFERENCE   4  (bases 1 to 1118)
  AUTHORS   Bhattarai,U., He,X., Xu,R., Liu,X., Pan,L., Sun,Y., Chen,J.X. and
            Chen,Y.
  TITLE     IL-12alpha deficiency attenuates pressure overload-induced cardiac
            inflammation, hypertrophy, dysfunction, and heart failure
            progression
  JOURNAL   Front Immunol 14, 1105664 (2023)
   PUBMED   36860846
  REMARK    GeneRIF: IL-12alpha deficiency attenuates pressure overload-induced
            cardiac inflammation, hypertrophy, dysfunction, and heart failure
            progression.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1118)
  AUTHORS   Li,S., Mirlekar,B., Johnson,B.M., Brickey,W.J., Wrobel,J.A.,
            Yang,N., Song,D., Entwistle,S., Tan,X., Deng,M., Cui,Y., Li,W.,
            Vincent,B.G., Gale,M. Jr., Pylayeva-Gupta,Y. and Ting,J.P.
  TITLE     STING-induced regulatory B cells compromise NK function in cancer
            immunity
  JOURNAL   Nature 610 (7931), 373-380 (2022)
   PUBMED   36198789
REFERENCE   6  (bases 1 to 1118)
  AUTHORS   Murphy,T.L., Cleveland,M.G., Kulesza,P., Magram,J. and Murphy,K.M.
  TITLE     Regulation of interleukin 12 p40 expression through an NF-kappa B
            half-site
  JOURNAL   Mol Cell Biol 15 (10), 5258-5267 (1995)
   PUBMED   7565674
REFERENCE   7  (bases 1 to 1118)
  AUTHORS   Heinzel,F.P., Rerko,R.M., Ahmed,F. and Pearlman,E.
  TITLE     Endogenous IL-12 is required for control of Th2 cytokine responses
            capable of exacerbating leishmaniasis in normally resistant mice
  JOURNAL   J Immunol 155 (2), 730-739 (1995)
   PUBMED   7608551
REFERENCE   8  (bases 1 to 1118)
  AUTHORS   Liu,W. and Kurlander,R.J.
  TITLE     Analysis of the interrelationship between IL-12, TNF-alpha, and
            IFN-gamma production during murine listeriosis
  JOURNAL   Cell Immunol 163 (2), 260-267 (1995)
   PUBMED   7606797
REFERENCE   9  (bases 1 to 1118)
  AUTHORS   Smith,T.J., Ducharme,L.A. and Weis,J.H.
  TITLE     Preferential expression of interleukin-12 or interleukin-4 by
            murine bone marrow mast cells derived in mast cell growth factor or
            interleukin-3
  JOURNAL   Eur J Immunol 24 (4), 822-826 (1994)
   PUBMED   7512032
REFERENCE   10 (bases 1 to 1118)
  AUTHORS   Schoenhaut,D.S., Chua,A.O., Wolitzky,A.G., Quinn,P.M., Dwyer,C.M.,
            McComas,W., Familletti,P.C., Gately,M.K. and Gubler,U.
  TITLE     Cloning and expression of murine IL-12
  JOURNAL   J Immunol 148 (11), 3433-3440 (1992)
   PUBMED   1350290
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121306.12.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13862062.148346.1,
                                           SRR13422589.327713.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-236               AC121306.12        34936-35171         c
            237-278             AC121306.12        33979-34020         c
            279-320             AC121306.12        33864-33905         c
            321-464             AC121306.12        33630-33773         c
            465-1118            AC121306.12        30662-31315         c
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 31.92 cM"
     gene            1..1118
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="interleukin 12a"
                     /db_xref="GeneID:16159"
                     /db_xref="MGI:MGI:96539"
     exon            1..236
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            237..278
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            279..320
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     CDS             315..620
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="isoform 5 is encoded by transcript variant 5;
                     interleukin-12 subunit alpha; interleukin 12a p35 subunit;
                     CLMF p35; IL-12 subunit p35; cytotoxic lymphocyte
                     maturation factor 35 kDa subunit; IL-12 p35 subunit;
                     interleukin-12 p35 subunit"
                     /codon_start=1
                     /product="interleukin-12 subunit alpha isoform 5"
                     /protein_id="NP_001397348.1"
                     /db_xref="GeneID:16159"
                     /db_xref="MGI:MGI:96539"
                     /translation="MMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVA
                     IDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA"
     exon            321..464
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     exon            465..1118
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1096..1101
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="hexamer: AATAAA"
     polyA_site      1118
                     /gene="Il12a"
                     /gene_synonym="Il-12a; IL-12p35; Ll12a; p35"
                     /note="major polyA site"
ORIGIN      
        1 aaagagatga cggttcagag tggccagcct aggctctgct gatccactgc gatgcaggtc
       61 tcagagggaa ccagctgtct tgctgagaga ttcacctcag cttctcctta tgtttttccc
      121 aggccagaga aaaactgaaa cattattcct gcactgctga agacatcgat catgaagaca
      181 tcacacggga ccaaaccagc acattgaaga cctgtttacc actggaacta cacaagaacg
      241 agagttgcct ggctactaga gagacttctt ccacaacaag agggagctgc ctgcccccac
      301 agaagacgtc tttgatgatg accctgtgcc ttggtagcat ctatgaggac ttgaagatgt
      361 accagacaga gttccaggcc atcaacgcag cacttcagaa tcacaaccat cagcagatca
      421 ttctagacaa gggcatgctg gtggccatcg atgagctgat gcagtctctg aatcataatg
      481 gcgagactct gcgccagaaa cctcctgtgg gagaagcaga cccttacaga gtgaaaatga
      541 agctctgcat cctgcttcac gccttcagca cccgcgtcgt gaccatcaac agggtgatgg
      601 gctatctgag ctccgcctga aaggctcaag gccctctgcc acagcgccct cctcacacag
      661 ataggaaaca aagaaagatt cataagagtc aggtggtctt ggcctggtgg gccttaagct
      721 ccttcaggaa tctgttctcc catcacatct catctcccca aaggtggcac agctacctca
      781 gcatggtccc ctccatcgct tctctcatat tcactataca agttgtttgt aagttttcat
      841 caaaatattg ttaaggggcg aagacgtcct cccctcaatg tgttagcaga agagcaagaa
      901 ctgataagct attgtttttg tgccaaagtg tttatgaaaa cactcagtca ccccttattt
      961 aaaaatattt attgctatat tttatactca tgaaagtaca tgagcctatt tatatttatt
     1021 tattttctat ttattataat atttcttatc agatgaattt gaaacatttt gaaacatacc
     1081 ttattttgtg gttctaataa agtaatgtta tcacttca
//
