LOCUS       NM_010544               2476 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus Indian hedgehog (Ihh), transcript variant 1, mRNA.
ACCESSION   NM_010544
VERSION     NM_010544.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Song,C., Sawall,J.K., Ji,X., Song,F., Liao,X., Peng,R., Ren,H.,
            Koyama,E., Pacifici,M. and Long,F.
  TITLE     Tgfbeta signaling stimulates glycolysis to promote the genesis of
            synovial joint interzone in developing mouse embryonic limbs
  JOURNAL   Sci Adv 11 (2), eadq4991 (2025)
   PUBMED   39772668
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Moldovan,G.E., Massri,N., Vegter,E.L., Pauneto-Delgado,I.N.,
            Burns,G.W., Joshi,N., Gu,B., Arora,R. and Fazleabas,A.T.
  TITLE     YAP1 and WWTR1 are required for murine pregnancy initiation
  JOURNAL   Reproduction 169 (1), e240355 (2025)
   PUBMED   39503541
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2476)
  AUTHORS   Hata,K., Wakamori,K., Hirakawa-Yamamura,A., Ichiyama-Kobayashi,S.,
            Yamaguchi,M., Okuzaki,D., Takahata,Y., Murakami,T., Uzawa,N.,
            Yamashiro,T. and Nishimura,R.
  TITLE     Serinc5 Regulates Sequential Chondrocyte Differentiation by
            Inhibiting Sox9 Function in Pre-Hypertrophic Chondrocytes
  JOURNAL   J Cell Physiol 240 (1), e31490 (2025)
   PUBMED   39568258
REFERENCE   4  (bases 1 to 2476)
  AUTHORS   Almubarak,A., Zhang,Q., Zhang,C.H., Abdelwahab,N., Kume,T.,
            Lassar,A.B. and Berry,F.B.
  TITLE     FOXC1 and FOXC2 regulate growth plate chondrocyte maturation
            towards hypertrophy in the embryonic mouse limb skeleton
  JOURNAL   Development 151 (16) (2024)
   PUBMED   39012257
REFERENCE   5  (bases 1 to 2476)
  AUTHORS   Yoshida,S., Kawamura,A., Aoki,K., Wiriyasermkul,P., Sugimoto,S.,
            Tomiyoshi,J., Tajima,A., Ishida,Y., Katoh,Y., Tsukada,T.,
            Tsuneoka,Y., Yamada,K., Nagamori,S., Nakayama,K. and Yoshida,K.
  TITLE     Positive regulation of Hedgehog signaling via phosphorylation of
            GLI2/GLI3 by DYRK2 kinase
  JOURNAL   Proc Natl Acad Sci U S A 121 (28), e2320070121 (2024)
   PUBMED   38968120
REFERENCE   6  (bases 1 to 2476)
  AUTHORS   Valentini,R.P., Brookhiser,W.T., Park,J., Yang,T., Briggs,J.,
            Dressler,G. and Holzman,L.B.
  TITLE     Post-translational processing and renal expression of mouse Indian
            hedgehog
  JOURNAL   J Biol Chem 272 (13), 8466-8473 (1997)
   PUBMED   9079674
REFERENCE   7  (bases 1 to 2476)
  AUTHORS   Bitgood,M.J. and McMahon,A.P.
  TITLE     Hedgehog and Bmp genes are coexpressed at many diverse sites of
            cell-cell interaction in the mouse embryo
  JOURNAL   Dev Biol 172 (1), 126-138 (1995)
   PUBMED   7589793
REFERENCE   8  (bases 1 to 2476)
  AUTHORS   Marigo,V., Roberts,D.J., Lee,S.M., Tsukurov,O., Levi,T.,
            Gastier,J.M., Epstein,D.J., Gilbert,D.J., Copeland,N.G.,
            Seidman,C.E. et al.
  TITLE     Cloning, expression, and chromosomal location of SHH and IHH: two
            human homologues of the Drosophila segment polarity gene hedgehog
  JOURNAL   Genomics 28 (1), 44-51 (1995)
   PUBMED   7590746
REFERENCE   9  (bases 1 to 2476)
  AUTHORS   Chang,D.T., Lopez,A., von Kessler,D.P., Chiang,C., Simandl,B.K.,
            Zhao,R., Seldin,M.F., Fallon,J.F. and Beachy,P.A.
  TITLE     Products, genetic linkage and limb patterning activity of a murine
            hedgehog gene
  JOURNAL   Development 120 (11), 3339-3353 (1994)
   PUBMED   7720571
REFERENCE   10 (bases 1 to 2476)
  AUTHORS   Echelard,Y., Epstein,D.J., St-Jacques,B., Shen,L., Mohler,J.,
            McMahon,J.A. and McMahon,A.P.
  TITLE     Sonic hedgehog, a member of a family of putative signaling
            molecules, is implicated in the regulation of CNS polarity
  JOURNAL   Cell 75 (7), 1417-1430 (1993)
   PUBMED   7916661
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139023.15, AK144322.1 and AI323361.1.
            
            On Aug 25, 2015 this sequence version replaced NM_010544.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK144322.1, BC046984.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849377,
                                           SAMN00849380 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: experimental evidence
                                                (PMID:9079674)
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AC139023.15        142890-142971       c
            83-2466             AK144322.1         85-2468
            2467-2476           AI323361.1         1-10                c
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 38.55 cM"
     gene            1..2476
                     /gene="Ihh"
                     /gene_synonym="HHG-2"
                     /note="Indian hedgehog"
                     /db_xref="GeneID:16147"
                     /db_xref="MGI:MGI:96533"
     exon            1..773
                     /gene="Ihh"
                     /gene_synonym="HHG-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             459..1694
                     /gene="Ihh"
                     /gene_synonym="HHG-2"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; indian hedgehog protein"
                     /codon_start=1
                     /product="indian hedgehog protein isoform 1 preproprotein"
                     /protein_id="NP_034674.2"
                     /db_xref="CCDS:CCDS15061.2"
                     /db_xref="GeneID:16147"
                     /db_xref="MGI:MGI:96533"
                     /translation="MSPAWLRPRLRFCLFLLLLLLVPAARGCGPGRVVGSRRRPPRKL
                     VPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRL
                     MTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDR
                     NKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQVRLENGERV
                     ALSAVKPGDRVLAMGEDGTPTFSDVLIFLDREPNRLRAFQVIETQDPPRRLALTPAHL
                     LFIADNHTEPAAHFRATFASHVQPGQYVLVSGVPGLQPARVAAVSTHVALGSYAPLTR
                     HGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFPSLAWGSWTPSEGVHWYPQMLYRLG
                     RLLLEESTFHPLGMSGAGS"
     exon            774..1035
                     /gene="Ihh"
                     /gene_synonym="HHG-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..2468
                     /gene="Ihh"
                     /gene_synonym="HHG-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2443..2448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ihh"
                     /gene_synonym="HHG-2"
                     /note="hexamer: AATAAA"
     polyA_site      2468
                     /gene="Ihh"
                     /gene_synonym="HHG-2"
                     /note="major polyA site"
ORIGIN      
        1 ctcactcgac cccgggctgc gccgcagacg gcagcagctc ccgctctgcc cgagccgcct
       61 gaccgccggg ccggggtgct aaccgcgggt cccttcagtc cgccggccgg gccagcccag
      121 cccagcccgc cggccctcag ccccgccgcc cgccgccccc cgccgtcgcc gcgttgccaa
      181 aacaaacggg ccggcctatt tattggcggc cggcgagccg ggcagctcag agtcgaggcg
      241 ccgaggggga cagcacgccg ccaccagcca gggccccggg cccccgcccc gcacctgagt
      301 cccgtcggcc ttgagccgcg tcgcgctgcc catggcgccc ccgcatggag tccccaagag
      361 ccacccagac gcctgagtcc ccgaagctgt cccagccacg cgcccaccta tcagcccacc
      421 aggcgccctc gcccgctgct ctcccgggct acccggccat gtctcccgcc tggctccggc
      481 cccgactgcg gttctgtctg ttcctgctgc tgctgcttct ggtgccggcg gcgcggggct
      541 gcgggccggg ccgggtggtg ggcagccgcc ggaggccgcc tcgcaagctc gtgcctcttg
      601 cctacaagca gttcagcccc aacgtgccgg agaagaccct gggcgccagc gggcgctacg
      661 aaggcaagat cgcgcgcagc tctgagcgct tcaaagagct cacccccaac tacaatcccg
      721 acatcatctt caaggacgag gagaacacgg gtgccgaccg cctcatgacc cagcgctgca
      781 aggaccgtct gaactcactg gccatctctg tcatgaacca gtggcctggt gtgaaactgc
      841 gggtgaccga aggctgggat gaagatggcc atcactcaga ggagtcttta cactatgagg
      901 gccgcgcggt ggatatcacc acctcagacc gtgaccgaaa taagtatgga ctgctggcgc
      961 gcttagcagt ggaggccggc ttcgactggg tgtattacga gtccaaggcc cacgtgcatt
     1021 gctctgtcaa gtctgagcat tcggccgctg ccaagacagg tggctgcttt cctgccggag
     1081 cccaggtgcg cctagagaac ggggagcgtg tggccctgtc agctgtaaag ccaggagacc
     1141 gggtgctggc catgggggag gatgggaccc ccaccttcag tgatgtgctt attttcctgg
     1201 accgcgagcc aaaccggctg agagctttcc aggtcatcga gactcaggat cctccgcgtc
     1261 ggctggcgct cacgcctgcc cacctgctct tcattgcgga caatcataca gaaccagcag
     1321 cccacttccg ggccacattt gccagccatg tgcaaccagg ccaatatgtg ctggtatcag
     1381 gggtaccagg cctccagcct gctcgggtgg cagctgtctc cacccacgtg gcccttgggt
     1441 cctatgctcc tctcacaagg catgggacac ttgtggtgga ggatgtggtg gcctcctgct
     1501 ttgcagctgt ggctgaccac catctggctc agttggcctt ctggcccctg cgactgtttc
     1561 ccagtttggc atggggcagc tggaccccaa gtgagggtgt tcactggtac cctcagatgc
     1621 tctaccgcct ggggcgtctc ttgctagaag agagcacctt ccatccactg ggcatgtctg
     1681 gggcaggaag ctgaagggac tctaaccact gccctcctgg aactgctgtg ctggatccaa
     1741 aggcctcctc accaggaagg ctctggccct ggaaggcacc tggcctgagg ttgtctccgt
     1801 cctctgtgcc agagtggaga caccattgag acttgaccag gtttgctggg ccccgaacct
     1861 tcatcttggt gtagagctgt gaactgagct gacaagcgtg tggtaggctc tcttttccta
     1921 gagaccgtaa gacccagcta gctctggctg cgattcttca cacgcattcc atctgtcttt
     1981 ggactgctta ctccaatgtt tctcggggcc tgggattgtg actttactgt tggcaactga
     2041 tcacagtatg aagagaggct gcccgtagat gggcttgcac ctcagtcgat gctgctagat
     2101 tcccttgaag ccagcaggga gctggctgga ctcattgcct cccagaactg aaagaccaca
     2161 gccagcctgg acatcccgaa acatgacctt ccctgcaggc cacactcctc cagcctcctg
     2221 agcctttgct gtcaatgggc ggagtctctg atccgggcaa tgtgacccta ctacctggga
     2281 ctggggaagg ggccttgcca cccctgccca ggctaagctc cttcccctgc taaaccacac
     2341 ccccacctcc tcctcctcca gtctgtctcc ttcaccctat ttatttgcat ggagggggaa
     2401 atccatggga gaattttggg aatgttttgg tctttctttt gtaataaaaa ttatttaagt
     2461 tgttagaaaa aaaaaa
//
