LOCUS       NM_001122737            3706 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus insulin-like growth factor 2 (Igf2), transcript
            variant 3, mRNA.
ACCESSION   NM_001122737
VERSION     NM_001122737.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Basu,S., Mautner,L., Whiting,K., Hasenpusch-Theil,K., Borkowska,M.
            and Theil,T.
  TITLE     Identification of Pappa and Sall3 as Gli3 direct target genes
            acting downstream of cilia signaling in corticogenesis
  JOURNAL   Cereb Cortex 34 (12) (2024)
   PUBMED   39716738
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Sandovici,I., Fernandez-Twinn,D.S., Campbell,N., Cooper,W.N.,
            Sekita,Y., Zvetkova,I., Ferland-McCollough,D., Prosser,H.M.,
            Oyama,L.M., Pantaleao,L.C., Cimadomo,D., Barbosa de Queiroz,K.,
            Cheuk,C.S.K., Smith,N.M., Kay,R.G., Antrobus,R., Hoelle,K.,
            Ma,M.K.L., Smith,N.H., Geyer,S.H., Reissig,L.F., Weninger,W.J.,
            Siddle,K., Willis,A.E., Lam,B.Y.H., Bushell,M., Ozanne,S.E. and
            Constancia,M.
  TITLE     Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and
            leads to developmental growth restriction and metabolic dysfunction
            in mice
  JOURNAL   Cell Rep 43 (9), 114750 (2024)
   PUBMED   39283743
REFERENCE   3  (bases 1 to 3706)
  AUTHORS   Sandovici,I., Knee,O., Lopez-Tello,J., Shreeve,N., Fowden,A.L.,
            Sferruzzi-Perri,A.N. and Constancia,M.
  TITLE     A genetically small fetus impairs placental adaptations near term
  JOURNAL   Dis Model Mech 17 (8) (2024)
   PUBMED   39207227
REFERENCE   4  (bases 1 to 3706)
  AUTHORS   Flaherty,S.E. 3rd, Bezy,O., Paulhus,B.L., Song,L., Piper,M.,
            Pang,J., Park,Y., Asano,S., Lien,Y.C., Griffin,J.D., Robertson,A.,
            Opsahl,A., Shanthappa,D.H., Ahn,Y., Pashos,E., Simmons,R.A.,
            Birnbaum,M.J. and Wu,Z.
  TITLE     SPAG7 deletion causes intrauterine growth restriction, resulting in
            adulthood obesity and metabolic dysfunction
  JOURNAL   Elife 12, 91114 (2024)
   PUBMED   39056292
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3706)
  AUTHORS   Matsuzaki,H., Kimura,M., Morihashi,M. and Tanimoto,K.
  TITLE     Imprinted DNA methylation of the H19 ICR is established and
            maintained in vivo in the absence of Kaiso
  JOURNAL   Epigenetics Chromatin 17 (1), 20 (2024)
   PUBMED   38840164
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3706)
  AUTHORS   Ferguson,M.W., Sharpe,P.M., Thomas,B.L. and Beck,F.
  TITLE     Differential expression of insulin-like growth factors I and II
            (IGF I and II), mRNA, peptide and binding protein 1 during mouse
            palate development: comparison with TGF beta peptide distribution
  JOURNAL   J Anat 181 (Pt 2) (Pt 2), 219-238 (1992)
   PUBMED   1284245
REFERENCE   7  (bases 1 to 3706)
  AUTHORS   Zemel,S., Bartolomei,M.S. and Tilghman,S.M.
  TITLE     Physical linkage of two mammalian imprinted genes, H19 and
            insulin-like growth factor 2
  JOURNAL   Nat Genet 2 (1), 61-65 (1992)
   PUBMED   1303252
REFERENCE   8  (bases 1 to 3706)
  AUTHORS   Rappolee,D.A., Sturm,K.S., Behrendtsen,O., Schultz,G.A.,
            Pedersen,R.A. and Werb,Z.
  TITLE     Insulin-like growth factor II acts through an endogenous growth
            pathway regulated by imprinting in early mouse embryos
  JOURNAL   Genes Dev 6 (6), 939-952 (1992)
   PUBMED   1317321
REFERENCE   9  (bases 1 to 3706)
  AUTHORS   Hannon,K., Smith,C.K. 2nd, Bales,K.R. and Santerre,R.F.
  TITLE     Temporal and quantitative analysis of myogenic regulatory and
            growth factor gene expression in the developing mouse embryo
  JOURNAL   Dev Biol 151 (1), 137-144 (1992)
   PUBMED   1374351
REFERENCE   10 (bases 1 to 3706)
  AUTHORS   Brown,E.J., Hsiao,D. and Rosenthal,S.M.
  TITLE     Induction and peak gene expression of insulin-like growth factor II
            follow that of myogenin during differentiation of BC3H-1 muscle
            cells
  JOURNAL   Biochem Biophys Res Commun 183 (3), 1084-1089 (1992)
   PUBMED   1314574
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC013548.13 and BC058615.1.
            
            On Oct 7, 2015 this sequence version replaced NM_001122737.1.
            
            Summary: This gene encodes a member of the insulin-like growth
            factor (IGF) family of proteins that promote growth and development
            during fetal and postnatal life. It is an imprinted gene that is
            expressed only from the paternal allele. The encoded protein
            undergoes proteolytic processing to generate a mature peptide. The
            transgenic overexpression of this gene in mice results in prenatal
            overgrowth, polyhydramnios, fetal and neonatal lethality,
            disproportionate organ overgrowth including tongue enlargement, and
            skeletal abnormalities. Mice lacking the encoded protein exhibit
            growth deficiency. Alternative splicing results in multiple
            transcript variants encoding different isoforms that may undergo
            similar processing to generate mature protein. [provided by RefSeq,
            Oct 2015].
            
            Transcript Variant: This variant (3) uses an alternate exon at its
            5' terminus and uses a downstream in-frame start codon, compared to
            variant 1. This results in a protein (isoform 2) with a shorter
            N-terminus, compared to isoform 1. Variants 2, 3, 4 and 5 encode
            the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M14951.1, CF736555.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 14645508
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC013548.13        57926-57955
            31-907              BC058615.1         1-877
            908-3706            AC013548.13        61856-64654
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 87.99 cM"
     gene            1..3706
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /note="insulin-like growth factor 2"
                     /db_xref="GeneID:16002"
                     /db_xref="MGI:MGI:96434"
     exon            1..109
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /inference="alignment:Splign:2.1.0"
     exon            110..272
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..658
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /note="isoform 2 preproprotein is encoded by transcript
                     variant 3; insulin-like growth factor II;
                     multiplication-stimulating polypeptide"
                     /codon_start=1
                     /product="insulin-like growth factor 2 isoform 2
                     preproprotein"
                     /protein_id="NP_001116209.1"
                     /db_xref="CCDS:CCDS52458.1"
                     /db_xref="GeneID:16002"
                     /db_xref="MGI:MGI:96434"
                     /translation="MGIPVGKSMLVLLISLAFALCCIAAYGPGETLCGGELVDTLQFV
                     CSDRGFYFSRPSSRANRRSRGIVEECCFRSCDLALLETYCATPAKSERDVSTSQAVLP
                     DDFPRYPVGKFFQYDTWRQSAGRLRRGLPALLRARRGRMLAKELKEFREAKRHRPLIV
                     LPPKDPAHGGASSEMSSNHQ"
     exon            273..421
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /inference="alignment:Splign:2.1.0"
     exon            422..3706
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3686..3691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /note="hexamer: AATAAA"
     polyA_site      3706
                     /gene="Igf2"
                     /gene_synonym="Igf-2; Igf-II; M6pr; Mpr; Peg2"
                     /note="major polyA site"
ORIGIN      
        1 ggcactgacc agttcgcaaa ctggacatta gcttctcctg tgagaacctt ccagcctttt
       61 cctgtcttca tcctcttcca gccccagcgg cctccttacc caacttcagg taccaatggg
      121 gatcccagtg gggaagtcga tgttggtgct tctcatctct ttggccttcg ccttgtgctg
      181 catcgctgct tacggccccg gagagactct gtgcggaggg gagcttgttg acacgcttca
      241 gtttgtctgt tcggaccgcg gcttctactt cagcaggcct tcaagccgtg ccaaccgtcg
      301 cagccgtggc atcgtggaag agtgctgctt ccgcagctgc gacctggccc tcctggagac
      361 atactgtgcc acccccgcca agtccgagag ggacgtgtct acctctcagg ccgtacttcc
      421 ggacgacttc cccagatacc ccgtgggcaa gttcttccaa tatgacacct ggagacagtc
      481 cgcgggacgc ctgcgcagag gcctgcctgc cctcctgcgt gcccgccggg gtcgcatgct
      541 tgccaaagag ctcaaagagt tcagagaggc caaacgtcat cgtcccctga tcgtgttacc
      601 acccaaagac cccgcccacg ggggagcctc ttcggagatg tccagcaacc atcagtgaat
      661 caaattatgt ggtaattctg caatgtagta ccatcaatct gtgacctcct cttgagcagg
      721 gacagttcca tcacgtccca cactaagatc tctctgctcc actccctccc caggtttctc
      781 cttacccacc cccatgcccc gcctccccac atcaggctgt tccccttgcc ccacaccatc
      841 gggcaagggg atctcagcag ttctaaaaaa ccaaatttga ttggctctaa acaacccaat
      901 tgacaccccc caaattatat attcacatta aaaaaaactt taaaaactac agtcccttta
      961 aaaacaaatt ggcttttaag aaattccata actgataatc taaaaattaa ataactaaag
     1021 aaattaattg gctaaaaaca tactaaaaat taattggctt aaaaacaatt ggcaaaatca
     1081 aataattcgg cgcccccccc ccttcatctt ctttccattt ggatctttag tcaaattggc
     1141 taagacttgg atcccagaac ccaagaagaa aggaagggga cccaaaattt tgcaggtggc
     1201 atgtcattgc ttcagtgccc tctccttatc ccaagcacct ttagtgtaaa gctggctgag
     1261 aacaacaata gccgcccaaa ctctttcttc actggtcatt ccatcacaaa tgtcccccat
     1321 gtcaccaagg gggctgggtg aaagaaccca aggagaagaa cagaacatga aaactggaaa
     1381 tagaacttaa ttggcacaag cccccagtcc caaaatctca cttttcccta cctaccctaa
     1441 aaagcacatg attatactca cacccacagg cattaacaca cacatacaca taacacatat
     1501 gcacacacac acacacacac acacacacac acacacagag gtatgcacac ccacataatt
     1561 ggatgaaaac attgaaatgg ctgaacaact tcgatttgaa ccacattccc caaaacaagg
     1621 cccatcccaa atttcctgag cagtttgcat ggtttgagct ctcctcccaa tccatctagt
     1681 ttctgctgct gcttattggc tctatgtgtg accaggctgc tagttctgtc tttctcagtg
     1741 agggaggtgt tctagcaagg ctcaattcca tctaagcacc tgtccacatg gtgagcccgg
     1801 tgctccccaa ccacccccgc catgaggacc aatgactagc cagtgtagaa taagtttggc
     1861 cagataagga gatggcactg ccaagtgata catgctgccc aagtaacctg acccctagtt
     1921 gtgctcctgg gaggaaagat ctgggggaca acccctaccc tgagcacacc tatgggccat
     1981 ctttgtcagt ctcctgggga gcccccgctt tttaggggct ccccaggaga ctcacactga
     2041 tgtggggagt gtgggaagtc tggcggttgg aggggtgggt ggggggcagt gggggctggg
     2101 tggggggagc tatgggtagg aagtggtccc agagaggttt taggtggaag aatcaggagg
     2161 agtcacaggt caacttgcag aattactgaa gaattaggac cccaaatttt atgccaattg
     2221 atctattccc ctctttttat ttctggggcc ggttttttcc tttttttttt aatccctcct
     2281 tagcttttta tgcgctcata atcaattgta cccattccct acataacggg agcagtgatc
     2341 aggtaatgaa tgcatcgagc catcaacacc agctagagcc atcaacaccg gctaccacaa
     2401 tgtcctgctc tccacaacct tgattttttt ttttatctct ctctatcgct tggcctgagt
     2461 tgggagtgga gtctctgtgg ggtgctggcc acgcacccac agagaaataa aaggaattga
     2521 gaaggtcgct acctggcctg acttctgggg acagtggctg gtccccagaa gttctgagga
     2581 gtggaggggg cgtggggcag tgtcccctca ggtgttagga aggtgctcgg aggccacaaa
     2641 agatggggct cccagctggc cactgccagt tgggggggaa ggggatagag atgtgagagt
     2701 agacaggtta catcaggcgg gagcaagtgg ctgccttctg agcatttggg ggagaacctc
     2761 tcccgtccct cagtgtcatc ttgcccactc ctcagcaccc caccttgccc ccaggaggtc
     2821 tggagctcta cagacctcct gggggcaagg tggggtgagg cccggagttg gggaagctag
     2881 gaggcttaaa gcctacagag ccaggagaac tgtatacacg gggtcatctg ggccctgggg
     2941 cctgagggtc tggcgagccg tagcagccac tccacggtgc ctaggactgc ggcggggaac
     3001 agggcggctg gaggtttacc tcacccccac ttctgcttcc agtgcagtcc ccctgcccaa
     3061 cagtccaact agcaatctag aggcctgagg cttctgggcc caggtgacag gactggcacc
     3121 accctggggg cggtgtgtgt cagccaagca tggcacagag ggtccctcag caagtgccta
     3181 aagaatgggc catttggaac attggacaga aactcaaaga gtaaattgtt ataattggag
     3241 actatgaatt ggcctggtat ccaaaacatc tcgaggcacc ctaaattacc tgcccatttg
     3301 gctggacacc cacccagtgt taatatgcct cgtgggatgg gtgctttcag gggcatttgc
     3361 tgaccaccct ctgtgtcccc acatttgcag ttctccccat cataggtcac cttgatgcag
     3421 gcacctccct ggcctcctat gcctagtgtg gccctccatc ttgtctcttc cctactgtct
     3481 tcagtgggat cccctcttgg gtccctccct ttgtcatcat gtgaagactt cccacgcgtc
     3541 gaacgccata tgtcacctgt gccactgtcc atgtcatcca gcagtggccc caggtgtttg
     3601 cctcaactca gtccctctaa catgcatttt ctggcaaaat ccaaagcttg ggttttgttt
     3661 ttaacctgtt aacgctcgca aacctaataa agcatttaaa atacta
//
