LOCUS       NM_026592               2332 bp    mRNA    linear   ROD 09-FEB-2024
DEFINITION  Mus musculus intraflagellar transport associated protein (Iftap),
            transcript variant 1, mRNA.
ACCESSION   NM_026592
VERSION     NM_026592.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Takahara,M., Kunii,M., Nakamura,K., Harada,A., Hirano,T., Katoh,Y.
            and Nakayama,K.
  TITLE     C11ORF74 interacts with the IFT-A complex and participates in
            ciliary BBSome localization
  JOURNAL   J Biochem 165 (3), 257-267 (2019)
   PUBMED   30476139
REFERENCE   3  (bases 1 to 2332)
  AUTHORS   Majkowski,M., Laszkiewicz,A., Sniezewski,L., Grzmil,P.,
            Pawlicka,B., Tomczyk,I., Michniewicz,M., Kapusniak,V., Janik,S.,
            Chodaczek,G. and Cebrat,M.
  TITLE     Lack of NWC protein (c11orf74 homolog) in murine spermatogenesis
            results in reduced sperm competitiveness and impaired ability to
            fertilize egg cells in vitro
  JOURNAL   PLoS One 13 (12), e0208649 (2018)
   PUBMED   30521625
  REMARK    GeneRIF: NWC-devoid spermatogenesis produces functionally impaired
            sperm. Sperm of knockout males fail to successfully compete with
            control sperm. NWC knockout sperm had a reduced ability to
            fertilize cumulus-intact eggs.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2332)
  AUTHORS   Kasztura,M., Sniezewski,L., Laszkiewicz,A., Majkowski,M., Kobak,K.,
            Peczek,K., Janik,S., Kapusniak,V., Miazek,A., Cebrat,M. and
            Kisielow,P.
  TITLE     Search for the Function of NWC, Third Gene Within RAG Locus:
            Generation and Characterization of NWC-Deficient Mice
  JOURNAL   Arch Immunol Ther Exp (Warsz) 64 (4), 311-319 (2016)
   PUBMED   26703212
  REMARK    GeneRIF: functional inactivation of NWC does not cause any gross
            developmental, physiological or reproductive abnormalities, and
            that under physiological conditions NWC may be involved in
            assembling and functioning of cilia
REFERENCE   5  (bases 1 to 2332)
  AUTHORS   Laszkiewicz,A., Bzdzion,L., Kasztura,M., Sniezewski,L., Janik,S.,
            Kisielow,P. and Cebrat,M.
  TITLE     Ikaros and RAG-2-mediated antisense transcription are responsible
            for lymphocyte-specific inactivation of NWC promoter
  JOURNAL   PLoS One 9 (9), e106927 (2014)
   PUBMED   25198102
  REMARK    GeneRIF: Ikaros, a lymphocyte-specific transcription factor, acts
            as a repressor of NWC promoter--thus identifying a new Ikaros
            target--but is insufficient for inducing methylation which depends
            on antisense transcription driven by RAG-2 promoter
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2332)
  AUTHORS   Laszkiewicz,A., Sniezewski,L., Kasztura,M., Bzdzion,L., Cebrat,M.
            and Kisielow,P.
  TITLE     Bidirectional activity of the NWC promoter is responsible for RAG-2
            transcription in non-lymphoid cells
  JOURNAL   PLoS One 7 (9), e44807 (2012)
   PUBMED   22984564
  REMARK    GeneRIF: Bidirectional activity of the NWC promoter is responsible
            for RAG-2 transcription in non-lymphoid cells
REFERENCE   7  (bases 1 to 2332)
  AUTHORS   Laszkiewicz,A., Cebrat,M., Miazek,A. and Kisielow,P.
  TITLE     Complexity of transcriptional regulation within the Rag locus:
            identification of a second Nwc promoter region within the Rag2
            intron
  JOURNAL   Immunogenetics 63 (3), 183-187 (2011)
   PUBMED   21243488
  REMARK    GeneRIF: Careful analysis of the Rag2 intergenic region uncovered a
            second evolutionarily conserved Nwc promoter region from which a
            previously unknown Nwc transcript can be generated in
            nonlymphocytes of Nwctmpro1 and normal mice.
REFERENCE   8  (bases 1 to 2332)
  AUTHORS   Cebrat,M., Cebula,A., Laszkiewicz,A., Kasztura,M., Miazek,A. and
            Kisielow,P.
  TITLE     Mechanism of lymphocyte-specific inactivation of RAG-2 intragenic
            promoter of NWC: implications for epigenetic control of RAG locus
  JOURNAL   Mol Immunol 45 (8), 2297-2306 (2008)
   PUBMED   18166226
  REMARK    GeneRIF: These results indicate that accessibility of NWC promoter
            and RAG promoters to trans-acting factors is regulated by different
            epigenetic mechanisms.
REFERENCE   9  (bases 1 to 2332)
  AUTHORS   Cebrat,M., Miazek,A. and Kisielow,P.
  TITLE     Identification of a third evolutionarily conserved gene within the
            RAG locus and its RAG1-dependent and -independent regulation
  JOURNAL   Eur J Immunol 35 (7), 2230-2238 (2005)
   PUBMED   15971274
  REMARK    GeneRIF: NWC is regulated in lymphocytes by RAG1 promoter, in all
            other cells by RAG2 intragenic promoter
REFERENCE   10 (bases 1 to 2332)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY777225.1, AK051679.1 and BE946529.1.
            
            [WARNING] On Apr 11, 2024 this sequence was replaced by
            NM_026592.5.
            
            On Apr 25, 2018 this sequence version replaced NM_026592.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051679.1, CB233850.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                BY777225.1         2-46
            46-2314             AK051679.1         2-2270
            2315-2332           BE946529.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 53.83 cM"
     gene            1..2332
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /note="intraflagellar transport associated protein"
                     /db_xref="GeneID:68170"
                     /db_xref="MGI:MGI:1915420"
     exon            1..151
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /inference="alignment:Splign:2.1.0"
     exon            152..303
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..905
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /note="isoform 1 is encoded by transcript variant 1;
                     uncharacterized protein C11orf74 homolog"
                     /codon_start=1
                     /product="intraflagellar transport-associated protein
                     isoform 1"
                     /protein_id="NP_080868.1"
                     /db_xref="CCDS:CCDS16461.1"
                     /db_xref="GeneID:68170"
                     /db_xref="MGI:MGI:1915420"
                     /translation="MLAQIPRPEIMDEDQLMKEVLDKFVNCHEQTYEEFLSTFTHLSK
                     EDNVAKWGAHGTDSSENIFSTVKWNHKTESNGRHFRNTSIFLHTSSEHSEEDQIVIDE
                     GHKVGSSFQGDMTRAGKVKVDNFLDLEDVDMEEESKPQMNKDCLLLPGEVEQDISTSV
                     PSYDPSVDLPLTSEVRPKPAVGRTQKQTEEILGDEVQPFFLDEEFDYDNVTLTPKFTA
                     GEIATIQELSKQKRVGSGTNVCELHD"
     exon            304..461
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /inference="alignment:Splign:2.1.0"
     exon            462..533
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /inference="alignment:Splign:2.1.0"
     exon            534..600
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /inference="alignment:Splign:2.1.0"
     exon            601..740
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /inference="alignment:Splign:2.1.0"
     exon            741..2316
                     /gene="Iftap"
                     /gene_synonym="B230118H07Rik; NWC"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggaatccgt gttctatggg ctgtgtagtt ggataaattg tcttcgctgc ttcatgacag
       61 aaaccttata ggctgtttga ctggataagg actccaaacg tgcgaaggga ctagatggac
      121 gccatggtga cggacagcta gggaacggga gagtggcctt gtgaattgaa atgcttgctc
      181 aaataccgag gccagagata atggatgaag atcagttaat gaaagaagtc ttggataagt
      241 ttgtcaactg ccatgagcaa acctatgaag aatttctgag cactttcact catctttcaa
      301 aagaggataa tgtggccaaa tggggagcac atggaactga ttcatcagaa aatatatttt
      361 ctactgtaaa atggaatcat aaaactgaat caaatggtcg tcattttaga aatacatcca
      421 tctttcttca cacttcatcg gaacactcag aagaggacca gattgtgatt gatgaaggcc
      481 acaaagttgg gagttccttt caaggtgata tgacccgggc tggaaaggtg aaggtagaca
      541 acttcctaga cttagaagat gtggacatgg aggaagagag taagccccag atgaacaagg
      601 attgcctgct acttccaggt gaggtggaac aggatataag caccagcgtt ccatcttatg
      661 atccttctgt ggacctaccg ctcacctctg aggtgaggcc aaagcctgct gtgggaagga
      721 ctcagaaaca aaccgaagag atacttggag atgaagttca gcccttcttt ctcgatgaag
      781 aatttgatta tgacaatgtg actctcactc ctaagtttac tgctggagag atagcaacca
      841 tccaagagct atctaagcaa aagagagtgg gttccggtac aaacgtgtgc gaactccatg
      901 actgatgtct ctgggtttag ttttattttg ttcacactca agcgacatta gaataaagca
      961 tatgtcaact gattccctct gtggaaatgt acgcggctgt attcatttgt cactgttgtt
     1021 tatcaccagg acacagatta atcaagacca aagagatcgc tttctcttct gtcccatggc
     1081 catgtcctgt gactgcttgg gacgatggaa gagggaaatg gggcaggaga taagggatgc
     1141 tgaatctgca tgtgattaag gcaccataca aggacttgag tgtgttgtgc tgaagactgg
     1201 gaatgtatgt gcatttttta gaggtgaagg acaatcacac gctccaaaca ttaccaggac
     1261 taacagaatc acatatgtat gtagggcttt ggtcctgtgt gtccggagat ggtggattca
     1321 gtgtggttgt ggagagactg agcttcactg aaaaggaagg cactgcagag ctgattttga
     1381 tgtctcacat ggaattctgg aacttaaaaa agttatcagt aaatcatatc tgacagttac
     1441 aaagtagagt gagtaggctc tgtctccaga agtttaagaa cagctagaga atgtaaaatg
     1501 gtattatggg gctggtgaga gggctctgta gaggtgcttg ctaggcaagc ctgttgacct
     1561 tagttagtcc ctggaactac gtaaaggggg acagagagaa atgactctat aaagctgtcc
     1621 tctgacctcc acaggcgcaa aggcaagcgc accccccttc tcctactaat aataaaagat
     1681 aaaaatttta aaatgtacca tggagtagta actctgcaga tgactcataa ggtcaccgag
     1741 ggaacgagta gagagggata caggcactga agaaggataa ggtgggcaat ctgtatgaaa
     1801 taatatttat tattgaggaa atcagcaagt gtgcttggta ttctttcttc ttttaagtag
     1861 cctttacatt ttatcatcgt cttaggaagc atagttgccg tccgttccct ttctctgatc
     1921 ccctacactt aagccacaca ccacagagta tgcttgttgc tgcttggttt tgagtctttc
     1981 ctatttctgt tccttttcgt ctcttgtggt aagaagacat gagagccacc atcttcaatg
     2041 cttgggcaca cagttcaata ttgcaaacca tgtacacaat attgtggcgt atctctagga
     2101 cttacccacc tgctaccgaa accacactca gttctccatg gcttctgttt ctgagttcga
     2161 ctgctggaga caccccatgg aaatggacat gcattgtctg ccctttggtg tctcgcttat
     2221 ttcacttatg acaatattct ctgggttcct tcacatggtc acacgtggca ggatttgttc
     2281 cttctgaatg ctgaataaag agtgtcacat catgtaaaaa aaaaaaaaaa aa
//
