LOCUS       NM_025931               1003 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus intraflagellar transport 27 (Ift27), mRNA.
ACCESSION   NM_025931
VERSION     NM_025931.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Huang,Q., Li,W., Zhou,Q., Awasthi,P., Cazin,C., Yap,Y.,
            Mladenovic-Lucas,L., Hu,B., Jeyasuria,P., Zhang,L., Granneman,J.G.,
            Hess,R.A., Ray,P.F., Kherraf,Z.E., Natarajan,V. and Zhang,Z.
  TITLE     Leucine zipper transcription factor-like 1 (LZTFL1), an
            intraflagellar transporter protein 27 (IFT27) associated protein,
            is required for normal sperm function and male fertility
  JOURNAL   Dev Biol 477, 164-176 (2021)
   PUBMED   34023333
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Desai,P.B., San Agustin,J.T., Stuck,M.W., Jonassen,J.A., Bates,C.M.
            and Pazour,G.J.
  TITLE     Ift25 is not a cystic kidney disease gene but is required for early
            steps of kidney development
  JOURNAL   Mech Dev 151, 10-17 (2018)
   PUBMED   29626631
  REMARK    GeneRIF: This work indicates that IFT25 and IFT27 are important
            players in the early development of the kidney and suggest that
            duplex kidney is part of the ciliopathy spectrum.
REFERENCE   3  (bases 1 to 1003)
  AUTHORS   Zhang,Y., Liu,H., Li,W., Zhang,Z., Shang,X., Zhang,D., Li,Y.,
            Zhang,S., Liu,J., Hess,R.A., Pazour,G.J. and Zhang,Z.
  TITLE     Intraflagellar transporter protein (IFT27), an IFT25 binding
            partner, is essential for male fertility and spermiogenesis in mice
  JOURNAL   Dev Biol 432 (1), 125-139 (2017)
   PUBMED   28964737
  REMARK    GeneRIF: Findings suggest that like IFT25, IFT27, even though not
            required for ciliogenesis in somatic cells, is essential for sperm
            flagella formation, sperm function, and male fertility in mice.
            IFT25 and IFT27 control sperm formation/function through many
            common mechanisms, but IFT25 has additional roles beyond IFT27.
REFERENCE   4  (bases 1 to 1003)
  AUTHORS   Liu,H., Li,W., Zhang,Y., Zhang,Z., Shang,X., Zhang,L., Zhang,S.,
            Li,Y., Somoza,A.V., Delpi,B., Gerton,G.L., Foster,J.A., Hess,R.A.,
            Pazour,G.J. and Zhang,Z.
  TITLE     IFT25, an intraflagellar transporter protein dispensable for
            ciliogenesis in somatic cells, is essential for sperm flagella
            formation
  JOURNAL   Biol Reprod 96 (5), 993-1006 (2017)
   PUBMED   28430876
REFERENCE   5  (bases 1 to 1003)
  AUTHORS   Xin,D., Christopher,K.J., Zeng,L., Kong,Y. and Weatherbee,S.D.
  TITLE     IFT56 regulates vertebrate developmental patterning by maintaining
            IFTB complex integrity and ciliary microtubule architecture
  JOURNAL   Development 144 (8), 1544-1553 (2017)
   PUBMED   28264835
REFERENCE   6  (bases 1 to 1003)
  AUTHORS   Follit,J.A., San Agustin,J.T., Jonassen,J.A., Huang,T.,
            Rivera-Perez,J.A., Tremblay,K.D. and Pazour,G.J.
  TITLE     Arf4 is required for Mammalian development but dispensable for
            ciliary assembly
  JOURNAL   PLoS Genet 10 (2), e1004170 (2014)
   PUBMED   24586199
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1003)
  AUTHORS   Ishikawa,H., Ide,T., Yagi,T., Jiang,X., Hirono,M., Sasaki,H.,
            Yanagisawa,H., Wemmer,K.A., Stainier,D.Y., Qin,H., Kamiya,R. and
            Marshall,W.F.
  TITLE     TTC26/DYF13 is an intraflagellar transport protein required for
            transport of motility-related proteins into flagella
  JOURNAL   Elife 3, e01566 (2014)
   PUBMED   24596149
  REMARK    Erratum:[Elife. 2014 Apr 01;3:e02897. doi: 10.7554/eLife.02897..
            PMID: 24692455]
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1003)
  AUTHORS   Howard,P.W., Jue,S.F. and Maurer,R.A.
  TITLE     Interaction of mouse TTC30/DYF-1 with multiple intraflagellar
            transport complex B proteins and KIF17
  JOURNAL   Exp Cell Res 319 (14), 2275-2281 (2013)
   PUBMED   23810713
REFERENCE   9  (bases 1 to 1003)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   10 (bases 1 to 1003)
  AUTHORS   Follit,J.A., Xu,F., Keady,B.T. and Pazour,G.J.
  TITLE     Characterization of mouse IFT complex B
  JOURNAL   Cell Motil Cytoskeleton 66 (8), 457-468 (2009)
   PUBMED   19253336
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC017514.1 and CO039411.1.
            
            On Jul 12, 2018 this sequence version replaced NM_025931.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BI412289.1, BC017514.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-987               BC017514.1         28-1014
            988-1003            CO039411.1         1-16                c
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 36.92 cM"
     gene            1..1003
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /note="intraflagellar transport 27"
                     /db_xref="GeneID:67042"
                     /db_xref="MGI:MGI:1914292"
     exon            1..313
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /inference="alignment:Splign:2.1.0"
     CDS             280..840
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /EC_number="3.6.5.2"
                     /note="RAB, member of RAS oncogene family-like 4; rab-like
                     protein 4"
                     /codon_start=1
                     /product="intraflagellar transport protein 27 homolog"
                     /protein_id="NP_080207.1"
                     /db_xref="CCDS:CCDS37131.1"
                     /db_xref="GeneID:67042"
                     /db_xref="MGI:MGI:1914292"
                     /translation="MVKLAAKCILAGDPAVGKTALVQMFRSDGTHFQKNYTLTTGVDL
                     VVKTVPVLDTNDSVELFIFDSAGKELFSEMLDKLWENPNVLCLVYDVTNEQSFISCTK
                     WLEKVRSQTSGISLPGVLVGTKTDLAGRQTVDSAQAQVWALSQGLEFFETSVKEMDNY
                     EAPFHCLAKQFYQLYREKVDIFHTLV"
     exon            314..393
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /inference="alignment:Splign:2.1.0"
     exon            394..453
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /inference="alignment:Splign:2.1.0"
     exon            454..513
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /inference="alignment:Splign:2.1.0"
     exon            514..631
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /inference="alignment:Splign:2.1.0"
     exon            632..741
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /inference="alignment:Splign:2.1.0"
     exon            742..990
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      969..974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /note="hexamer: AATAAA"
     polyA_site      987
                     /gene="Ift27"
                     /gene_synonym="2600013G09Rik; Rabl4"
                     /note="major polyA site"
ORIGIN      
        1 agcctgtttg gcgcgactct tgggaccaga ggactagtgg ttgtgtctca actcttcttt
       61 gggccgggtc taaggctgga cttggaacgg gttctgctcc gccccaccgg aacctttgca
      121 ggctgcctgg ggcctgaagc cctccgtcca cccaacccta gcacccgggc catcatccat
      181 ccctccatca cggcctcagg ccctggtgtc agctgtcccc gaatcctaac cccctttcag
      241 cgtcccccac ccccacgtct ccgctagtac ctggttacta tggtgaagct agctgccaaa
      301 tgcatcctgg caggggaccc agccgtaggc aagacggccc tggtgcagat gttccgcagc
      361 gacgggaccc atttccagaa gaactacacc ctgacaactg gagtggattt ggtggtgaag
      421 acagtgccag ttcttgacac aaatgacagt gtggaactct tcatttttga ctcagccggc
      481 aaggagctgt tctctgaaat gctggataag ttgtgggaga atcccaacgt cctgtgcctt
      541 gtctatgatg tgaccaatga acagtccttc atcagctgca ccaagtggtt ggagaaggtc
      601 cggtctcaga cttcaggcat ctccctccca ggtgttttag tgggaactaa gacagacctg
      661 gctggccgac aaacagtgga ttcagctcag gcccaggtat gggcactgag tcaaggcctg
      721 gaattctttg agacatctgt gaaggagatg gataattacg aggccccgtt ccactgtctg
      781 gccaagcagt tctaccagct gtaccgggaa aaagtggaca tattccatac cctggtgtga
      841 gggtacagtg gactgtcctg aagggctgcc cactctgccg ctgctgctgt gagcccagca
      901 gaccattgtc ttttcaggag acagaagacg ggatcacctg ggatttctca gtatccacca
      961 tggttttcaa taaaatgaga agagcaacaa aaaaaaaaaa aaa
//
