LOCUS       NM_028394                640 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus intraflagellar transport 25 (Ift25), transcript
            variant 1, mRNA.
ACCESSION   NM_028394 XM_204090 XM_915730 XM_979355
VERSION     NM_028394.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 640)
  AUTHORS   Wang,J.J., Ge,W., Zhai,Q.Y., Liu,J.C., Sun,X.W., Liu,W.X., Li,L.,
            Lei,C.Z., Dyce,P.W., De Felici,M. and Shen,W.
  TITLE     Single-cell transcriptome landscape of ovarian cells during
            primordial follicle assembly in mice
  JOURNAL   PLoS Biol 18 (12), e3001025 (2020)
   PUBMED   33351795
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 640)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 640)
  AUTHORS   Desai,P.B., San Agustin,J.T., Stuck,M.W., Jonassen,J.A., Bates,C.M.
            and Pazour,G.J.
  TITLE     Ift25 is not a cystic kidney disease gene but is required for early
            steps of kidney development
  JOURNAL   Mech Dev 151, 10-17 (2018)
   PUBMED   29626631
  REMARK    GeneRIF: This work indicates that IFT25 and IFT27 are important
            players in the early development of the kidney and suggest that
            duplex kidney is part of the ciliopathy spectrum.
REFERENCE   5  (bases 1 to 640)
  AUTHORS   Zhang,Y., Liu,H., Li,W., Zhang,Z., Shang,X., Zhang,D., Li,Y.,
            Zhang,S., Liu,J., Hess,R.A., Pazour,G.J. and Zhang,Z.
  TITLE     Intraflagellar transporter protein (IFT27), an IFT25 binding
            partner, is essential for male fertility and spermiogenesis in mice
  JOURNAL   Dev Biol 432 (1), 125-139 (2017)
   PUBMED   28964737
  REMARK    GeneRIF: fFndings suggest that like IFT25, IFT27, even though not
            required for ciliogenesis in somatic cells, is essential for sperm
            flagella formation, sperm function, and male fertility in mice.
            IFT25 and IFT27 control sperm formation/function through many
            common mechanisms, but IFT25 has additional roles beyond IFT27.
REFERENCE   6  (bases 1 to 640)
  AUTHORS   Yang,N., Li,L., Eguether,T., Sundberg,J.P., Pazour,G.J. and Chen,J.
  TITLE     Intraflagellar transport 27 is essential for hedgehog signaling but
            dispensable for ciliogenesis during hair follicle morphogenesis
  JOURNAL   Development 142 (12), 2194-2202 (2015)
   PUBMED   26023097
  REMARK    Erratum:[Development. 2015 Aug 15;142(16):2860. doi:
            10.1242/dev.128751.. PMID: 26286943]
REFERENCE   7  (bases 1 to 640)
  AUTHORS   Eguether,T., San Agustin,J.T., Keady,B.T., Jonassen,J.A., Liang,Y.,
            Francis,R., Tobita,K., Johnson,C.A., Abdelhamed,Z.A., Lo,C.W. and
            Pazour,G.J.
  TITLE     IFT27 links the BBSome to IFT for maintenance of the ciliary
            signaling compartment
  JOURNAL   Dev Cell 31 (3), 279-290 (2014)
   PUBMED   25446516
REFERENCE   8  (bases 1 to 640)
  AUTHORS   Ishikawa,H., Ide,T., Yagi,T., Jiang,X., Hirono,M., Sasaki,H.,
            Yanagisawa,H., Wemmer,K.A., Stainier,D.Y., Qin,H., Kamiya,R. and
            Marshall,W.F.
  TITLE     TTC26/DYF13 is an intraflagellar transport protein required for
            transport of motility-related proteins into flagella
  JOURNAL   Elife 3, e01566 (2014)
   PUBMED   24596149
  REMARK    Erratum:[Elife. 2014 Apr 01;3:e02897. doi: 10.7554/eLife.02897..
            PMID: 24692455]
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 640)
  AUTHORS   Keady,B.T., Samtani,R., Tobita,K., Tsuchya,M., San Agustin,J.T.,
            Follit,J.A., Jonassen,J.A., Subramanian,R., Lo,C.W. and Pazour,G.J.
  TITLE     IFT25 links the signal-dependent movement of Hedgehog components to
            intraflagellar transport
  JOURNAL   Dev Cell 22 (5), 940-951 (2012)
   PUBMED   22595669
  REMARK    GeneRIF: IFT25 links the signal-dependent movement of Hedgehog
            components to intraflagellar transport.
REFERENCE   10 (bases 1 to 640)
  AUTHORS   Follit,J.A., Xu,F., Keady,B.T. and Pazour,G.J.
  TITLE     Characterization of mouse IFT complex B
  JOURNAL   Cell Motil Cytoskeleton 66 (8), 457-468 (2009)
   PUBMED   19253336
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA000737.1, BY309412.1, AA939607.1 and BB759869.1.
            
            On Sep 16, 2017 this sequence version replaced NM_028394.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CK331982.1, AK157651.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                AA000737.1         1-36
            37-93               BY309412.1         15-71
            94-586              AA939607.1         48-540
            587-640             BB759869.1         406-459
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 50.18 cM"
     gene            1..640
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /note="intraflagellar transport 25"
                     /db_xref="GeneID:72938"
                     /db_xref="MGI:MGI:1920188"
     exon            1..93
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /inference="alignment:Splign:2.1.0"
     exon            94..206
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..540
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /note="heat shock protein beta-11; placental protein 25
                     homolog; heat shock protein family B (small), member 11"
                     /codon_start=1
                     /product="intraflagellar transport protein 25 homolog"
                     /protein_id="NP_082670.1"
                     /db_xref="CCDS:CCDS18435.1"
                     /db_xref="GeneID:72938"
                     /db_xref="MGI:MGI:1920188"
                     /translation="MRKVDLCSVTEGTEVILATSSDEKHPPENIIDGNPETFWTTTGM
                     FPQEFIICFHKHVKIEKLVIQSYLVRTLRIEKTTSKEPLDFEQWVEKDLVHTEGQLQN
                     EEIVARDGYATFLRFIIVSAFDHFASVHSISAEGLTVSSLP"
     exon            207..313
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /inference="alignment:Splign:2.1.0"
     exon            314..382
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /inference="alignment:Splign:2.1.0"
     exon            383..426
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /inference="alignment:Splign:2.1.0"
     exon            427..640
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      574..579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
                     /note="hexamer: AATATA"
     polyA_site      595
                     /gene="Ift25"
                     /gene_synonym="2900042B11Rik; Hspb11; PP25"
ORIGIN      
        1 ggcggggatc gcgagcggac ccggtccttt cgcggagcta cccaaggccc aggcgagcgg
       61 gtccgtgcag cacggtgggc gggcaccgaa gaggctaacc attttgaaat gaggaaagtg
      121 gatctctgct cagtcactga agggacagaa gtgattctgg ccacatcgag tgatgaaaag
      181 cacccacctg aaaacatcat tgatgggaat ccggaaacct tttggaccac cacaggcatg
      241 tttccccagg agttcattat ttgttttcac aaacacgtaa agattgaaaa gcttgtgata
      301 caaagttact tggtgcggac tttgaggatt gaaaagacca catctaaaga gccattggat
      361 tttgagcagt gggttgaaaa agatttagta cacacagagg ggcagcttca aaatgaagaa
      421 attgtggcac gagatggcta cgccactttc ttgagattca ttattgtctc agcatttgat
      481 cattttgcat ctgtgcacag catttctgca gagggactaa cagtctcaag tcttccttag
      541 taattacaac aaaatgctct gaactacttt taaaatatat ttattgaaat acaaagctat
      601 ctttgatatg tttactatta aatagatttg tatcactctg
//
