LOCUS       NM_018854               1136 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus intraflagellar transport 20 (Ift20), mRNA.
ACCESSION   NM_018854
VERSION     NM_018854.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ye,J.W., Abbas,T., Zhou,J.T., Chen,J., Yang,M.L., Huang,X.H.,
            Zhang,H., Ma,H., Ma,A., Xu,B., Murtaza,G., Shi,Q.H. and Shi,B.L.
  TITLE     Homozygous CCDC146 mutation causes oligoasthenoteratozoospermia in
            humans and mice
  JOURNAL   Zool Res 45 (5), 1073-1087 (2024)
   PUBMED   39245651
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1136)
  AUTHORS   Kretschmer,V., Schneider,S., Matthiessen,P.A., Reichert,D.,
            Hotaling,N., Glassser,G., Lieberwirth,I., Bharti,K., De Cegli,R.,
            Conte,I., Nandrot,E.F. and May-Simera,H.L.
  TITLE     Deletion of IFT20 exclusively in the RPE ablates primary cilia and
            leads to retinal degeneration
  JOURNAL   PLoS Biol 21 (12), e3002402 (2023)
   PUBMED   38048369
  REMARK    GeneRIF: Deletion of IFT20 exclusively in the RPE ablates primary
            cilia and leads to retinal degeneration.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1136)
  AUTHORS   Ma,Y., Wu,B., Chen,Y., Ma,S., Wang,L., Han,T., Lin,X., Yang,F.,
            Liu,C., Zhao,J. and Li,W.
  TITLE     CCDC146 is required for sperm flagellum biogenesis and male
            fertility in mice
  JOURNAL   Cell Mol Life Sci 81 (1), 1 (2023)
   PUBMED   38038747
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1136)
  AUTHORS   Jeong,J., Kang,I., Kim,Y., Ku,K.B., Park,J.H., Kim,H.J., Kim,C.W.,
            La,J., Jung,H.E., Kim,H.C., Choi,Y.J., Kim,J., Kim,J. and Lee,H.K.
  TITLE     Regulation of c-SMAC formation and AKT-mTOR signaling by the
            TSG101-IFT20 axis in CD4+ T cells
  JOURNAL   Cell Mol Immunol 20 (5), 525-539 (2023)
   PUBMED   37029318
  REMARK    GeneRIF: Regulation of c-SMAC formation and AKT-mTOR signaling by
            the TSG101-IFT20 axis in CD4[+] T cells.
REFERENCE   6  (bases 1 to 1136)
  AUTHORS   Follit,J.A., Tuft,R.A., Fogarty,K.E. and Pazour,G.J.
  TITLE     The intraflagellar transport protein IFT20 is associated with the
            Golgi complex and is required for cilia assembly
  JOURNAL   Mol Biol Cell 17 (9), 3781-3792 (2006)
   PUBMED   16775004
  REMARK    GeneRIF: This work suggests that IFT20 functions in the delivery of
            ciliary membrane proteins from the Golgi complex to the cilium.
REFERENCE   7  (bases 1 to 1136)
  AUTHORS   Jurczyk,A., Gromley,A., Redick,S., San Agustin,J., Witman,G.,
            Pazour,G.J., Peters,D.J. and Doxsey,S.
  TITLE     Pericentrin forms a complex with intraflagellar transport proteins
            and polycystin-2 and is required for primary cilia assembly
  JOURNAL   J Cell Biol 166 (5), 637-643 (2004)
   PUBMED   15337773
REFERENCE   8  (bases 1 to 1136)
  AUTHORS   Baker,S.A., Freeman,K., Luby-Phelps,K., Pazour,G.J. and
            Besharse,J.C.
  TITLE     IFT20 links kinesin II with a mammalian intraflagellar transport
            complex that is conserved in motile flagella and sensory cilia
  JOURNAL   J Biol Chem 278 (36), 34211-34218 (2003)
   PUBMED   12821668
REFERENCE   9  (bases 1 to 1136)
  AUTHORS   Pazour,G.J., Baker,S.A., Deane,J.A., Cole,D.G., Dickert,B.L.,
            Rosenbaum,J.L., Witman,G.B. and Besharse,J.C.
  TITLE     The intraflagellar transport protein, IFT88, is essential for
            vertebrate photoreceptor assembly and maintenance
  JOURNAL   J Cell Biol 157 (1), 103-113 (2002)
   PUBMED   11916979
REFERENCE   10 (bases 1 to 1136)
  AUTHORS   Mink,M., Fogelgren,B., Olszewski,K., Maroy,P. and Csiszar,K.
  TITLE     A novel human gene (SARM) at chromosome 17q11 encodes a protein
            with a SAM motif and structural similarity to
            Armadillo/beta-catenin that is conserved in mouse, Drosophila, and
            Caenorhabditis elegans
  JOURNAL   Genomics 74 (2), 234-244 (2001)
   PUBMED   11386760
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591177.14.
            
            On Mar 1, 2018 this sequence version replaced NM_018854.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK139942.1, AK002397.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               AL591177.14        75494-75709
            217-445             AL591177.14        77643-77871
            446-574             AL591177.14        77967-78095
            575-660             AL591177.14        79093-79178
            661-764             AL591177.14        80028-80131
            765-1136            AL591177.14        80494-80865
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 46.74 cM"
     gene            1..1136
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /note="intraflagellar transport 20"
                     /db_xref="GeneID:55978"
                     /db_xref="MGI:MGI:1915585"
     exon            1..216
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /inference="alignment:Splign:2.1.0"
     exon            217..445
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /inference="alignment:Splign:2.1.0"
     exon            446..574
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /inference="alignment:Splign:2.1.0"
     CDS             448..846
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /note="uterine protein; intraflagellar transport 20
                     homolog"
                     /codon_start=1
                     /product="intraflagellar transport protein 20 homolog"
                     /protein_id="NP_061342.1"
                     /db_xref="CCDS:CCDS25111.1"
                     /db_xref="GeneID:55978"
                     /db_xref="MGI:MGI:1915585"
                     /translation="MAKDILGEAGLHFDELNKLRVLDPEVTQQTVELKEECKDFVDKI
                     GQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLKSIAKQREAQQQQLQALIAEKKT
                     QLERYRVEYEALCKVEAEQNEFIDQFIFQK"
     exon            575..660
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /inference="alignment:Splign:2.1.0"
     exon            661..764
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /inference="alignment:Splign:2.1.0"
     exon            765..1136
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      923
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /note="major polyA site"
     regulatory      1104..1109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /note="hexamer: AATAAA"
     polyA_site      1136
                     /gene="Ift20"
                     /gene_synonym="0610009H04Rik; mIFT20"
                     /note="major polyA site"
ORIGIN      
        1 gagcaaaggc ttctgggcct agtgggcagt tatcgcgtgt tgcatctatt tcccagcaag
       61 caccgcaggg tgggcggatg ctggtgcttc tggactcggg ggtgacgtgt ggctcctaga
      121 tcttgtctcc gtggcagcag ccggttgcag ttgccggaga gaagcttcta ccgacagtcg
      181 tcagggccgc agagcgctgc cgaggcggct cgtcagctaa gctgtgctgc tccagggcag
      241 gcaccatgtt gcattcatct ttacgcccag catctagcag tgtcggcatg tagtaggcac
      301 tcaagaaatg tatgttgaat gaacgatgtc tgtgacaagc aactggactt tcctctttcc
      361 tgacccttgc tcctatgaca tacctcctga ttgccactgt caccccttca aagcagacct
      421 ctagggcttc ctggaaggga aacagctatg gccaaggaca tcttgggcga ggcagggctg
      481 cattttgatg aactgaacaa gctccgagtg ttggacccag aggtcaccca gcagactgtg
      541 gagctcaagg aggagtgcaa ggactttgtg gacaaaattg gccagtttca gaaaattgtt
      601 ggtggtctaa ttgagcttgt tgatcagctt gccaaagaag cagagaacga gaagatgaag
      661 gccattggtg ctcggaactt gctgaagtcc atagcgaagc agagagaagc ccagcagcag
      721 cagctgcagg ccctgatagc agaaaagaag acgcagctag aaaggtatcg ggttgaatat
      781 gaagctttgt gtaaagtaga agcagaacaa aatgaattta ttgaccaatt tatttttcag
      841 aaatgaactg aaaatttcag ttttatagca ggaaggcaaa aaaaaaaaaa aaaaaaaaaa
      901 aaaaagcacc ccaaccaaaa aaacaaacct ctgtaccatt ccagtgatgt cactgatgtg
      961 cgcatgcagt gttacggggg cagctgataa acagtgcaca gcctctgcta gtggccaggc
     1021 agcacagggc ttcagctcga cttctcagca caaactcagt gtctgtaaac tttggtacat
     1081 tcacaaagct ctcaaggaaa agcaataaat accatttgca agtggcttgg tctttc
//
