LOCUS       NM_031156               5105 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus insulin degrading enzyme (Ide), mRNA.
ACCESSION   NM_031156 XM_974712
VERSION     NM_031156.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Zhu,S., Waeckel-Enee,E., Oshima,M., Moser,A., Bessard,M.A.,
            Gdoura,A., Roger,K., Mode,N., Lipecka,J., Yilmaz,A., Bertocci,B.,
            Diana,J., Saintpierre,B., Guerrera,I.C., Scharfmann,R.,
            Francesconi,S., Mauvais,F.X. and van Endert,P.
  TITLE     Islet cell stress induced by insulin-degrading enzyme deficiency
            promotes regeneration and protection from autoimmune diabetes
  JOURNAL   iScience 27 (6), 109929 (2024)
   PUBMED   38799566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5105)
  AUTHORS   Corraliza-Gomez,M., Bermejo,T., Lilue,J., Rodriguez-Iglesias,N.,
            Valero,J., Cozar-Castellano,I., Arranz,E., Sanchez,D. and
            Ganfornina,M.D.
  TITLE     Insulin-degrading enzyme (IDE) as a modulator of microglial
            phenotypes in the context of Alzheimer's disease and brain aging
  JOURNAL   J Neuroinflammation 20 (1), 233 (2023)
   PUBMED   37817156
  REMARK    GeneRIF: Insulin-degrading enzyme (IDE) as a modulator of
            microglial phenotypes in the context of Alzheimer's disease and
            brain aging.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5105)
  AUTHORS   Merino,B., Casanueva-Alvarez,E., Quesada,I.,
            Gonzalez-Casimiro,C.M., Fernandez-Diaz,C.M., Postigo-Casado,T.,
            Leissring,M.A., Kaestner,K.H., Perdomo,G. and Cozar-Castellano,I.
  TITLE     Insulin-degrading enzyme ablation in mouse pancreatic alpha cells
            triggers cell proliferation, hyperplasia and glucagon secretion
            dysregulation
  JOURNAL   Diabetologia 65 (8), 1375-1389 (2022)
   PUBMED   35652923
  REMARK    GeneRIF: Insulin-degrading enzyme ablation in mouse pancreatic
            alpha cells triggers cell proliferation, hyperplasia and glucagon
            secretion dysregulation.
            Erratum:[Diabetologia. 2024 Jan;67(1):218-219. doi:
            10.1007/s00125-023-06037-9. PMID: 37966551]
REFERENCE   5  (bases 1 to 5105)
  AUTHORS   Fursht,O., Liran,M., Nash,Y., Medala,V.K., Ini,D., Royal,T.G.,
            Goldsmith,G., Nahary,L., Benhar,I. and Frenkel,D.
  TITLE     Antibody-Mediated Inhibition of Insulin-Degrading Enzyme Improves
            Insulin Activity in a Diabetic Mouse Model
  JOURNAL   Front Immunol 13, 835774 (2022)
   PUBMED   35350789
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5105)
  AUTHORS   Miller,B.C., Eckman,E.A., Sambamurti,K., Dobbs,N., Chow,K.M.,
            Eckman,C.B., Hersh,L.B. and Thiele,D.L.
  TITLE     Amyloid-beta peptide levels in brain are inversely correlated with
            insulysin activity levels in vivo
  JOURNAL   Proc Natl Acad Sci U S A 100 (10), 6221-6226 (2003)
   PUBMED   12732730
  REMARK    GeneRIF: findings indicate that there is an inverse correlation
            between in vivo insulysin activity levels and brain Abeta peptide
            levels and suggest that modulation of insulysin activity may alter
            the risk for Alzheimer's disease
REFERENCE   7  (bases 1 to 5105)
  AUTHORS   Farris,W., Mansourian,S., Chang,Y., Lindsley,L., Eckman,E.A.,
            Frosch,M.P., Eckman,C.B., Tanzi,R.E., Selkoe,D.J. and Guenette,S.
  TITLE     Insulin-degrading enzyme regulates the levels of insulin, amyloid
            beta-protein, and the beta-amyloid precursor protein intracellular
            domain in vivo
  JOURNAL   Proc Natl Acad Sci U S A 100 (7), 4162-4167 (2003)
   PUBMED   12634421
  REMARK    GeneRIF: Knockout mice show abnormal levels of insulin, amyloid
            beta protein and ABPP in vivo.
REFERENCE   8  (bases 1 to 5105)
  AUTHORS   Phillips,R.L., Ernst,R.E., Brunk,B., Ivanova,N., Mahan,M.A.,
            Deanehan,J.K., Moore,K.A., Overton,G.C. and Lemischka,I.R.
  TITLE     The genetic program of hematopoietic stem cells
  JOURNAL   Science 288 (5471), 1635-1640 (2000)
   PUBMED   10834841
REFERENCE   9  (bases 1 to 5105)
  AUTHORS   Watson,M.L., Rao,J.K., Gilkeson,G.S., Ruiz,P., Eicher,E.M.,
            Pisetsky,D.S., Matsuzawa,A., Rochelle,J.M. and Seldin,M.F.
  TITLE     Genetic analysis of MRL-lpr mice: relationship of the Fas apoptosis
            gene to disease manifestations and renal disease-modifying loci
  JOURNAL   J Exp Med 176 (6), 1645-1656 (1992)
   PUBMED   1460423
REFERENCE   10 (bases 1 to 5105)
  AUTHORS   Affholter,J.A., Hsieh,C.L., Francke,U. and Roth,R.A.
  TITLE     Insulin-degrading enzyme: stable expression of the human
            complementary DNA, characterization of its protein product, and
            chromosomal mapping of the human and mouse genes
  JOURNAL   Mol Endocrinol 4 (8), 1125-1135 (1990)
   PUBMED   2293021
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AJ278422.1, AK004972.2, BU698019.1, AK078930.1 and AA163502.1.
            
            On Mar 4, 2020 this sequence version replaced NM_031156.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ278422.1, BC041675.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AJ278422.1         1-21
            22-1213             AK004972.2         2-1193
            1214-1791           AK004972.2         1195-1772
            1792-1917           BU698019.1         206-331
            1918-3265           AK004972.2         1900-3247
            3266-3432           AK078930.1         129-295
            3433-4874           AK078930.1         297-1738
            4875-5105           AA163502.1         156-386
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 32.24 cM"
     gene            1..5105
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /note="insulin degrading enzyme"
                     /db_xref="GeneID:15925"
                     /db_xref="MGI:MGI:96412"
     exon            1..144
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..3106
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /EC_number="3.4.24.56"
                     /note="insulysin; insulinase; insulin protease"
                     /codon_start=1
                     /product="insulin-degrading enzyme"
                     /protein_id="NP_112419.4"
                     /db_xref="GeneID:15925"
                     /db_xref="MGI:MGI:96412"
                     /translation="MRNGLVWLLHPALPGTLRSILGARPPPAKRLCGFPKQTYSTMSN
                     PAIQRIEDQIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPN
                     IPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEG
                     ALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKF
                     GTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFS
                     EVENKNVPLPEFPEHPFQEEHLRQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYL
                     GHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIIL
                     HMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVL
                     TAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPED
                     IIQKWQNADLNGKFKLPTKNEFIPTNFEILSLEKDATPYPALIKDTAMSKLWFKQDDK
                     FFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTI
                     YGMYLSVKGYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHA
                     MYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALG
                     VMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTD
                     MQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPP
                     HYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQ
                     QYNYDRDNIEVAYLKTLTKDDIIRFYQEMLAVDAPRRHKVSVHVLAREMDSCPVVGEF
                     PSQNDINLSEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL"
     exon            145..329
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            330..537
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            538..707
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            708..830
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            831..943
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1106
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1199
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1291
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1372
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1476
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1579
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1702
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1785
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1930
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2041
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2162
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2254
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2366
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..2534
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2807
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2869
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..2942
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2943..3010
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3011..5105
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3247..3252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3266
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /note="major polyA site"
     regulatory      5083..5088
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5105
                     /gene="Ide"
                     /gene_synonym="1300012G03Rik; 4833415K22Rik"
                     /note="major polyA site"
ORIGIN      
        1 cgccaaaagg aagcgttcgc cgagatcgca gcggctgcgc cggggtatgc ggaacgggct
       61 cgtgtggctg ctgcaccccg cgctgcccgg caccttgcgc tccatcctcg gcgcccgccc
      121 gccgcccgcg aagcgactgt gtggattccc aaaacagact tacagcacaa tgagtaatcc
      181 ggccatccag agaatagaag accaaattgt caagtctcct gaagacaaac gggaataccg
      241 tggactagag ctggccaatg gcatcaaagt gcttctcatc agcgatccca ccacagacaa
      301 gtcctcagcg gccctcgatg tgcacatagg ttcactgtca gaccctccaa atattcctgg
      361 cttaagtcat ttttgtgaac atatgctgtt tttgggaacc aagaaatatc ctaaagaaaa
      421 tgaatatagc cagtttctca gtgaacatgc tggaagttca aatgcattca ccagtggaga
      481 acacaccaat tattatttcg atgtttccca tgaacacttg gaaggagccc tggacaggtt
      541 tgcgcagttt ttcctgtgcc ccttgtttga tgcaagttgt aaagacagag aggtgaacgc
      601 tgtcgattca gaacatgaga agaatgtgat gaacgatgcc tggagactct tccagctgga
      661 aaaggctacg gggaacccca aacacccctt cagcaaattt gggacaggaa acaaatatac
      721 tctagagact cggcccaacc aagaaggcat cgacgtaagg gaagagctct tgaaatttca
      781 ctctacgtat tattcgtcca atctgatggc gatttgtgtt ttaggtcgag aatccttaga
      841 cgacctcact aacctggtgg tgaagctgtt ctccgaggtg gagaacaaga acgttcctct
      901 gcctgagttc cctgagcacc ctttccagga agagcatctc aggcaacttt ataaaatagt
      961 acccattaag gatattagaa atctttatgt gacatttccc ataccagacc ttcagcagta
     1021 ctacaaatcc aatcctggtc attatcttgg tcatctaatt gggcacgaag gtcctggaag
     1081 cctgttgtca gaactcaaat caaagggctg ggtgaatacc ctggttggtg gacagaagga
     1141 aggagcccga ggttttatgt tttttatcat taatgtggac ttaactgaag aagggttatt
     1201 gcacgttgaa gatataattt tgcacatgtt tcaatacatt cagaagctac gtgcagaagg
     1261 acctcaagaa tgggttttcc aagagtgcaa ggacttgaat gctgttgctt tcagatttaa
     1321 agataaagag agaccacgag gctatacgtc caagattgca ggaaagttgc actattatcc
     1381 cctaaatgga gtgctcacag ctgaatatct attggaggaa tttagacccg acttgataga
     1441 catggttctt gataaactca gaccagaaaa tgtgcgggtg gcaatagttt ccaaatcatt
     1501 tgaagggaaa actgaccgca cagagcagtg gtatggaaca cagtataagc aagaagctat
     1561 cccagaggac atcattcaga aatggcaaaa tgctgacctt aatggaaaat ttaagcttcc
     1621 aacaaagaat gaatttattc ccacaaattt tgagattttg tcattagaaa aagatgcaac
     1681 accataccct gctcttatta aggatacagc catgagtaag ctgtggttca aacaagatga
     1741 taaatttttc ttgccgaaag cttgtctcaa ctttgaattt ttcagcccgt ttgcttatgt
     1801 ggaccccttg cactgtaaca tggcctattt gtaccttgag ctcctcaaag actcactcaa
     1861 cgagtatgca tatgcagcag agctagcagg cctgagctat gacctccaaa ataccatcta
     1921 tgggatgtat ctctcagtga aaggttacaa tgacaaacag ccaattttgc tgaagaagat
     1981 caccgagaaa atggctactt ttgagattga taagaagaga tttgaaatta tcaaagaggc
     2041 ctatatgcga tctcttaata acttccgggc tgagcagcct caccagcatg ccatgtacta
     2101 cctccgcttg ctgatgactg aagtggcctg gactaaagat gagctaaaag aagcccttga
     2161 cgatgttacc ctcccccgac ttaaggcctt catacctcag ctgctgtcac ggctgcatat
     2221 tgaagcactt ctccatggaa acataaccaa gcaggctgcc ttaggagtta tgcagatggt
     2281 agaagacact cttatcgagc atgctcacac caaacctctc cttccaagtc agctagtccg
     2341 gtacagagaa gtccagctcc ctgacagagg atggtttgtt tatcagcaga ggaatgaagt
     2401 tcacaataac tgcggcatcg agatctacta ccagacagac atgcagagca cctcggagaa
     2461 catgttcctg gagctgtttt gccagattat ctcggagcct tgcttcaata ccctgcgcac
     2521 caaggagcag cttggctata ttgtcttcag tggaccccgt cgggccaacg gtatccaagg
     2581 cttgcgcttc atcatccagt cagaaaaacc accccactac ctggagagca gagtagaagc
     2641 cttcttgatt accatggaaa aggccataga ggacatgaca gaggaggcgt tccaaaaaca
     2701 cattcaagca ctagcgattc ggcgactcga caaaccaaag aaactctctg cggagtgtgc
     2761 taaatactgg ggggaaatca tctcgcagca gtacaattac gacagagata acatagaagt
     2821 tgcatattta aagacactta ccaaggacga tatcatcaga ttctaccagg aaatgttggc
     2881 tgtcgacgca ccgaggagac ataaagtgtc tgtccatgtt cttgccaggg aaatggattc
     2941 ttgtcctgtt gttggagagt tcccctctca gaatgatata aacttgtccg aagcgccacc
     3001 cttgccacaa cctgaagtga ttcacaacat gactgagttc aagcgtggcc tgccactgtt
     3061 tcccctcgtg aaaccacata ttaacttcat ggctgcaaaa ctctgaagaa agtagacatg
     3121 ttcctgtgtc ttccagggcc tgggaaaaca gtctcagctt tgagtagttt ctggcttgct
     3181 attagagaga caaacagaaa agagttaatc aggcattatt atgtagaaat gttaaaaacc
     3241 caaagtaata aacttataaa atcttataga tgtagaatat ttttaaaatc tcttaaatct
     3301 cttaattttt ttccttttta ttcctaagag aaattccctt atattaactg cttaatctga
     3361 agaaagatat cccaatacag tctttcttcc ttattctgta aaatagtcac ttgtcttgaa
     3421 aaaaaaaaaa aagagctttt tttcttaaag gcttcaaaac atttagagag gattacattt
     3481 acctttttaa aaaccgatct tcacatctgc tttctatttg atggtttcct gtaaaccaac
     3541 agaaaacatt acatttgaca gatgatgcac aaagcaatgc catgttcctt tattagtgaa
     3601 cttgctgatt ttataaaatt tgggtatgtt ttttaatctt cttagtcaaa tgctggaagc
     3661 cccagaattc ccagtgctag aaacgcagac tgaccttaca cccagttcag aatttctcag
     3721 gattcctgtg ggctctcgct catcagattc tgttgacatt tctgtttctc gtagttggtt
     3781 tgctgtgttc tgtcagcaga cgtgctgtgc taagatcagc tgttgtaact ccccacaact
     3841 cctagggtga aattgtgatc cgtgtgtgaa ctaaaacatt tgcccctcta gggacttaaa
     3901 aggaagcaaa tacagagcca cctccttgga gatacaaaac ggttgcgttc tgaaatagcc
     3961 agtctccgta agactctaaa ctccccgctg cttccggtct catcttgcct taagtgttat
     4021 ttttttgaat atatgaatat aaacatacag atgatgactg gagtggactt ttaaaaaact
     4081 atttttttca caagatacta ttttaggtga aaatgttcct gtagatttaa cagctgtttt
     4141 aaagtatttg ctattaaaac ttgcttcaag aacaagcgtg gctatgtttc cacacacagg
     4201 caatagcaac agaaagcaaa gcgctcctgt tcgtccacca gctgaggccg agagtggcgt
     4261 ttccaggtga catcttcctg ccccatgtta cttgattggc caggattttc tgctgtagaa
     4321 tctctcggct tttcacaggt taagtttcac attttccaaa tacggtgaac ttgggcggct
     4381 gtggaggccg ctctccctgt ggtgctgcgc ccgtcttcta tccacatgtt ctaagagtct
     4441 cccggtgaga agcctgtctc tgtccatccc ttcagaagac acgagtcaac actgccttct
     4501 cctctgcgca actggcccag tagccaaggt cgactttgaa ctccttgatc ctcctgcctc
     4561 cacctctcaa gtgccgggat taggcttgcg ccaccccgcc cagtgtgact atgcagcttt
     4621 tcatcgaact gcaaaatcta tacaaagatg aatctaaact tagtcctgag tgttcgaatc
     4681 catcgttgta aacacaaaca acagtctgct accttttctg taaatgctca cctgccgttg
     4741 agtaacttag ctcttaaaaa aaaaatgggt acagttaaaa gtttgtcatt tgtatgagaa
     4801 aagacacctg aagagattgg aaacaatccc atgtcgtctt ccatgaacct gcatgtggtg
     4861 ttcagtgtgt tcccaagctc ctgtgtgtca cttggcatcc caaccggttc atctcagtgg
     4921 gtccaggttg tggtccatga acaagaacat actgtccaga gaccgcatgt acgtactcag
     4981 gcttccgcaa gtgagatgtg tgaaaaagtc acgagttgcc agagacttaa aaagtctttt
     5041 tagtcagtca gtgtggcggg gtcctgaggt tatcctgttt acaataaatc tgacctggaa
     5101 ctcgg
//
