LOCUS       NM_198653               3560 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus isoleucine-tRNA synthetase 2, mitochondrial (Iars2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_198653 XM_355261
VERSION     NM_198653.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK048653.1 and AK043590.1.
            
            On Apr 11, 2018 this sequence version replaced NM_198653.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048653.1, AK043590.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3540              AK048653.1         2-3541
            3541-3560           AK043590.1         3543-3562
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 89.48 cM"
     gene            1..3560
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /note="isoleucine-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:381314"
                     /db_xref="MGI:MGI:1919586"
     exon            1..381
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..3153
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /EC_number="6.1.1.5"
                     /note="isoleucyl-tRNA synthetase, mitochondrial; ileRS;
                     isoleucine--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="isoleucine--tRNA ligase, mitochondrial
                     precursor"
                     /protein_id="NP_941055.1"
                     /db_xref="CCDS:CCDS15596.1"
                     /db_xref="GeneID:381314"
                     /db_xref="MGI:MGI:1919586"
                     /translation="MHWGLCPRGPGAAAVAAAGSFWGPARLPSRLGCLGMTRRLVVRS
                     VAGADSPQSSSKGGRYRDTVLLPQTSFPMKLLGRQQSDMELEIQQKCGFSELYSWQRE
                     RKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMRGSKVHFVPGWDCHGLPI
                     ETKVLSELGVDAQSLSAMEIREKARSFAQAAIEKQKSAFVRWGVMADWNNCYYTFDPK
                     YEAKQLRVFYQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRSIYVRFPLL
                     RPPPKLESLTDASSPVSVLVWTTQPWTIPANQAICYMPEAKYAVVKCSASGHLYILAE
                     DKIAPVASALETTFDVVAAFSGVDLEGGTCSHPLTPDKVSPLLPATHVTMAKGTGLVH
                     TAPAHGMEDYSVASQHSLPMDCLVDEGGMFTDAAGPELQNKAVLKEGTDVVIKMLQAT
                     KNVLKEENIVHSYPCDWRTKTPVLIRASKQWFVNITDIKAAAKESLKTVKFIPGAALN
                     SMTDMLDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIIKLVEQHGSDVWW
                     TLPAEQLLPAEVLAQAGGPGALEYAPGQDILDIWFDSGTSWSCVLQDTQQRADLYLEG
                     KDQLGGWFQSSLLTSVATRSKAPFRTVMVHGFTLGEKGEKMSKSLGNVINPDTIISGG
                     KDHSKEPPYGADILRWWIAESNVFTEVTIGPSVLSAARDDISKLRNTLRFLLGNLTGF
                     NPETDSVPVKNMYVIDQYMLHLIQDFATKITDSYKQYDFGKVVRLLKAFYTRELSSFY
                     FSIVKDRLYCENEKDPKRRSCQTALAEILDVLVRAFAPILPHLAEEVFQHIPYVTEPK
                     SVFRTGWINTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYEVIIVIEPGLLFE
                     IMEMLQAEETSSTSQLNELMMASQTTLLAQEPRERTAGDIELTGTFVINLEGGDIREE
                     SSYKVIVVPTAREKCPRCWKHTSETADALCPRCAEVIGAK"
     exon            382..504
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            505..664
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            665..813
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            814..863
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            864..973
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1064
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1180
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1350
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1441
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1593
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1754
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1857
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..1951
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2060
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2163
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2289
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2290..2421
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2528
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2674
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2675..2865
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..3010
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3011..3560
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3479..3484
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3501
                     /gene="Iars2"
                     /gene_synonym="2010002H18Rik"
                     /note="major polyA site"
ORIGIN      
        1 gccccggagc gcgtgcgcag ttcctcgccg ccgagctctt caagggtctc tacctttagg
       61 taccgggcgg ctgccgtggg ccgtgtgcgg agggctccgc ccggctcgtc caccatgcac
      121 tgggggctgt gccctcgcgg gcccggggcg gcggcggtcg ccgcggctgg gagcttctgg
      181 gggccagccc gtttgcccag ccgcctaggg tgtctgggga tgacgaggag gctcgtggta
      241 cgatccgtgg ccggggcgga cagcccccag agcagctcga aaggcggcag ataccgtgac
      301 acggtgctgc tgccgcagac cagcttcccg atgaagctgc tgggccgcca gcagtccgac
      361 atggagctgg agatccagca gaaatgtggc ttttcagagc tttattcctg gcaaagagaa
      421 agaaaagtaa agacggaatt ttgccttcat gacgggcctc cttatgccaa cggtgaccct
      481 catgttgggc atgccctgaa caagattttg aaagacattg ccaatcgatt ccatatgatg
      541 agaggttcca aggtacactt tgtgcctggc tgggattgcc atggcttgcc cattgaaaca
      601 aaagtgttat cagagctcgg tgtagatgcc cagagtcttt cagccatgga gatcagggag
      661 aaagccagat catttgctca ggcagccatt gaaaagcaga aatcggcgtt tgtccgctgg
      721 ggagtgatgg cagactggaa taactgctat tacacatttg acccaaagta cgaagccaag
      781 cagctgagag tattctacca aatgtatgag aagggcttgg tttatcgatc ttacaaacca
      841 gtatattggt ctccctcatc aaggactgcg ttggctgaag cagagcttga atacaaccct
      901 gagcatgtca gtcggtcgat atacgtaaga ttccccctcc tgagacctcc tccgaagttg
      961 gagtctctta cagatgcctc atcccctgtt agcgttttag tttggaccac ccagccttgg
     1021 acaattcccg ccaaccaggc aatttgctat atgccagagg caaagtatgc tgtggtgaaa
     1081 tgttctgcct ctgggcacct ctacatcctg gctgaagata aaatagcacc tgtcgcttct
     1141 gccttggaaa caacatttga tgttgttgca gcattttcag gtgtggatct ggaaggtggc
     1201 acttgtagtc accctttaac tcccgataaa gtgtctcctc ttttacctgc tacccacgtg
     1261 actatggcca aaggaacagg gttggttcac acagccccag ctcacggtat ggaagattac
     1321 agcgtagcat ctcagcacag cctccctatg gactgtttag tggatgaagg cggaatgttc
     1381 accgatgccg caggccctga acttcagaac aaggctgtac ttaaagaagg aacagatgtg
     1441 gttataaaga tgcttcaagc taccaagaat gtgttgaaag aagagaacat tgtgcacagc
     1501 tatccctgtg actggagaac taagacaccc gtgctgattc gagccagcaa gcagtggttt
     1561 gtaaatatca cggacatcaa ggctgcagct aaggagtcgt tgaagacggt gaagttcatt
     1621 cccggagccg cactgaacag catgacggac atgctggaca gacggccgta ctggtgcata
     1681 tcccggcaga gagtctgggg ggttcccatt cctgtgtttc atcataagac aaaagatgaa
     1741 tatttgatca acagccaaac cactgagcat attattaaac tcgtcgagca gcacggcagt
     1801 gatgtgtggt ggaccctccc tgctgagcag cttctcccgg cagaagtcct agctcaggct
     1861 ggtggccctg gggctttgga atacgcgcca gggcaggaca ttttggacat ctggtttgac
     1921 agtggaactt catggtcttg tgttcttcaa gacacgcagc agagggcaga cttgtacctg
     1981 gagggaaagg accagctcgg gggctggttc cagtcttcct tactaacaag tgtggccacc
     2041 aggtcaaaag cacctttcag gacagtgatg gttcatggat ttacacttgg agaaaaagga
     2101 gaaaagatgt ccaagtccct cgggaatgtc attaatcctg acactatcat cagtggagga
     2161 aaagaccaca gcaaagaacc tccctacgga gctgacatcc tgcgctggtg gatagctgag
     2221 tccaatgtct tcacggaggt gaccattggc ccctctgtac tctctgctgc cagagatgac
     2281 atcagcaagc tcagaaacac actccgcttt ctcttgggaa atttgaccgg ttttaacccc
     2341 gagacagatt ctgttcccgt gaagaatatg tatgtcatag accagtatat gctgcactta
     2401 attcaggatt ttgcgacaaa gattactgac tcatacaaac agtatgactt tgggaaagtt
     2461 gtcaggctgt tgaaagcctt ctacaccaga gaactgtcca gcttttactt cagcatagtc
     2521 aaggataggc tctactgcga aaacgagaag gatcccaagc gccgctcgtg tcagacagca
     2581 ttggcagaaa ttttggatgt gttggtccgt gcttttgccc ccattcttcc tcacttggca
     2641 gaagaggtgt ttcagcacat tccctatgtt acagaaccca agagtgtttt tcgcactggc
     2701 tggattaata caagctctat ttggaagaag ccaggactgg aggaggcagt ggaaagtgcg
     2761 tgcgccatga gggactcctt tcttggcagc atccctggca aaaatgctgc tgagtacgag
     2821 gtcatcatag tgatcgagcc agggctgctg tttgagatca tggagatgct ccaagctgag
     2881 gagacctcta gcacgtctca gctgaatgag ctgatgatgg cttcccagac gacattgctg
     2941 gctcaggagc cacgggaaag aactgcaggt gacattgagc tcacggggac attcgtcatc
     3001 aatctagaag gtggtgacat tcgtgaggag tcttcatata aagtcattgt ggtaccgact
     3061 gccagagaga agtgccctcg atgttggaag catacctctg agactgcaga cgctctctgt
     3121 cctcggtgtg cagaggtcat tggtgcaaag tagcaacctg atgtcaacag acgacgacac
     3181 tccccacagt actgactggc tgaggagatg agctgttaga cagttcccta gaaagaaatc
     3241 caaggcttac acagaaagta gaggggcaga tagatggtag cagatggggt aagaatcaga
     3301 agattaaaag aatatttctt gtaactatag aaagaataat gtatgtatat atatatatat
     3361 ggagatatat atgcagatat acatatatac agggcacaca cacacacaca ctcatgactt
     3421 cttcagagac agacactgaa tatggttact ttcaatgtgg cagaatgttt tatattttat
     3481 taaaactttt ttgactcagt atttaagctc tatgatatct gaaaataaag gcattctggg
     3541 aaactcccag catatgatgg
//
