LOCUS       NM_172015               4396 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus isoleucyl-tRNA synthetase 1 (Iars1), mRNA.
ACCESSION   NM_172015
VERSION     NM_172015.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Watanabe,M., Shishido,K., Kanehira,N., Hiura,K., Nakano,K.,
            Okamura,T., Ando,R., Sasaki,H. and Sasaki,N.
  TITLE     Molecular and Pathological Analyses of IARS1-Deficient Mice: An
            IARS Disorder Model
  JOURNAL   Int J Mol Sci 24 (8), 6955 (2023)
   PUBMED   37108118
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Sansom,S.N., Griffiths,D.S., Faedo,A., Kleinjan,D.J., Ruan,Y.,
            Smith,J., van Heyningen,V., Rubenstein,J.L. and Livesey,F.J.
  TITLE     The level of the transcription factor Pax6 is essential for
            controlling the balance between neural stem cell self-renewal and
            neurogenesis
  JOURNAL   PLoS Genet 5 (6), e1000511 (2009)
   PUBMED   19521500
REFERENCE   3  (bases 1 to 4396)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   4  (bases 1 to 4396)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY139910.1, AK133856.1 and BB052629.1.
            
            On Jul 22, 2009 this sequence version replaced NM_172015.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC067029.1, SRR1660811.10450.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BY139910.1         9-22
            15-4386             AK133856.1         2-4373
            4387-4396           BB052629.1         261-270
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 25.38 cM"
     gene            1..4396
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /note="isoleucyl-tRNA synthetase 1"
                     /db_xref="GeneID:105148"
                     /db_xref="MGI:MGI:2145219"
     exon            1..74
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            75..200
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..3870
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /EC_number="6.1.1.5"
                     /note="isoleucyl-tRNA synthetase, cytoplasmic; IRS; ileRS;
                     isoleucine-tRNA synthetase"
                     /codon_start=1
                     /product="isoleucine--tRNA ligase, cytoplasmic"
                     /protein_id="NP_742012.2"
                     /db_xref="CCDS:CCDS36659.1"
                     /db_xref="GeneID:105148"
                     /db_xref="MGI:MGI:2145219"
                     /translation="MVQQVPENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFY
                     DGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGI
                     KGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVW
                     WVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTFPLEEDENT
                     SLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVARGKLFILTEARLSALYKQESDYEIL
                     ERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHR
                     VCMDFNIIQKDSVPVCPVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGT
                     FTHSYPFCWRSDTPLIYKSVPSWFVRVEPMVDQLLKNNDLCYWVPEFVREKRFGNWLK
                     EARDWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEELSGTKISDLHRESIDHLTI
                     PSRCGKAPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGIDQTRG
                     WFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALR
                     LYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFLYNE
                     HTVRESPNITDRWVLSFMQSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRR
                     RLKGESGVEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASLRDKDT
                     LSIHYLMLPRVREELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQD
                     PEALEDIRSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMM
                     AIKRLSNEELERFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVL
                     LDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLNNVIESHT
                     DFIFATIKAPLKPYPVPTSDNILIQEQTQLKGSELEITLTKGSCVPGPACAYVNLNIC
                     ANGTEQGGVLLLENPKGDNQLNLVKLKTVVTSVFGVKNAKLSVFHGETEIQNQTDLLS
                     LSGRTLCVTAGASPSPISSPSTLLCQYLNLQLLNAEPQECLTGTVGTLLLENPLGQNG
                     LTHQGLVHEAAKVFGLRSRRLRLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTADG
                     "
     exon            201..357
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            358..477
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            478..560
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            561..678
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            679..826
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            827..914
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            915..975
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1071
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1194
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1286
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1385
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1512
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..1586
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1781
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1868
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..1952
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2097
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2218
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2310
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2388
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2510
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2613
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2697
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2872
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..2985
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2986..3081
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3258
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3364
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3365..3490
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..3634
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3635..3787
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     exon            3788..4396
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4361..4366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /note="hexamer: ATTAAA"
     polyA_site      4389
                     /gene="Iars1"
                     /gene_synonym="2510016L12Rik; E430001P04Rik; Iars; ILRS"
                     /note="major polyA site"
ORIGIN      
        1 gtcagtattg catcacctcc agacgcggaa cgtggtcgca ggctgcaacc tgagcgagct
       61 ctccgggttc ccagcaacag aatggttcaa caagttccag aaaacatcag ttttcctgct
      121 gaagaagaaa aaatcttgga gttctggtcc aaacataact gtttccagga atgcctcaaa
      181 cagtcaaaac tcaggccaaa gtttaccttc tatgatgggc ctccctttgc tactggactg
      241 cctcactatg gacacatact tgctgggaca atcaaagata tcgttacgag atacgctcac
      301 cagagcgggt ttcacgttga cagaagattc gggtgggact gtcacggctt acctgtggag
      361 tacgaaatcg ataagacact gggcatcaaa ggcccggagg atgtggccaa aatggggatt
      421 gcagagtaca acaagcagtg ccgagcaatc gtgatgagat actccgctga gtggaagtct
      481 actgttacca gactcggccg atggatcgac tttgacaatg actacaaaac actataccca
      541 cagttcatgg aatcagtctg gtgggtcttc aagcagctgt atgacaaagg cctcgtgtac
      601 agaggcgtga aggtcatgcc cttctccacc gcgtgtggca ctccactctc caacttcgaa
      661 tctaaccaga attacaagga tgttcaggat ccctcagtat ttgtaacatt ccctttggag
      721 gaagatgaaa atacatcctt agttgcttgg acaaccactc cttggactct acccagtaac
      781 cttgcactat gcgtcaatcc agaaatacag tatgtgaaga tcaaagatgt ggccagggga
      841 aaattattca tcttaacgga agccagatta tcggccctct ataagcaaga gagcgactat
      901 gagatacttg aaaggtttcc tggtgcctct ctcaaaggca agaaatacaa gccgttgttt
      961 gactacttta ttaagtgtaa ggagaacggt gccttcaccg tgcttgtcga ccactatgtg
     1021 aaggatgaag aaggcacagg cgttgtgcac caggctcctt actttggtgc tgatgatcat
     1081 cgtgtctgta tggacttcaa cattattcag aaagactcag tccctgtttg tcctgtggat
     1141 gcttcaggct gcttcacaga ggaagtgaca cattttgtgg ggcagtatgt gaaggatgct
     1201 gacaaaaata tcattaggat gttgaaggag cagggccggc tgctggcggc gggcacgttc
     1261 actcacagct accctttctg ctggaggtca gacacgcccc tcatttacaa gtctgtgccc
     1321 agctggtttg tgcgggtgga gcccatggtg gaccagctac tgaagaacaa cgacctgtgc
     1381 tactgggtcc cagagtttgt acgagaaaaa cggtttggaa attggctgaa ggaggcccgt
     1441 gactgggcaa tttccagaaa cagatactgg ggcaccccca tcccactgtg ggtcagcgaa
     1501 gacttggagg aggtagtgtg cattggatca gtggcagagc ttgaagaact gtcgggaaca
     1561 aaaatctcag atctccacag agagagcatc gaccacctga ccatcccatc ccgctgcggg
     1621 aaagcaccat tgcggcgcgt ctctgaggtg tttgactgct ggttcgagag tggaagtatg
     1681 ccctatgccc aggtccatta tccgttcgag agcaagcgcg agtttgagga tgcctttcct
     1741 gcagacttca ttgctgaagg catcgatcaa accagaggat ggttttacac cctgctggtg
     1801 ctagccacag cgctctttgg acagccacct ttcaagaatg tgattgtgaa tggcctcatc
     1861 ttggcaagtg atggccaaaa aatgagcaag cggaaaaaga actacccaga tccagtttcc
     1921 atcatcgata agtatggtgc ggacgccctc aggttatatc tgatcaattc ccctgtggta
     1981 agagcagaaa acctgcgctt caaggaggaa ggtgtgcggg atgtcctcaa agatgtgctg
     2041 ctcccgtggt acaacgctta ccgcttcttc atccagaacg tctttaggct ccacaaggaa
     2101 gaagaagtga agttcctata caacgagcat acagtcagag aaagccctaa catcaccgac
     2161 cgctgggttc tgtccttcat gcagtcgctc cttggcttct ttgagaccga aatggcagct
     2221 tacaggctgt acactgtggt acctcgcctg gtcaagtttg tagatattct aaccaactgg
     2281 tatgtccgaa tgaacaggag gaggttaaag ggtgagagtg gggtggaaga ctgtgtcatg
     2341 gctctggaga cactgttcag tgtcctgctt tctctgtgca gactgatggc cccttacacg
     2401 ccctttctga ctgagctgat gtaccagaat ctaaagctgc tgattgatcc tgcttctctc
     2461 cgggacaagg acacactcag catccactac ctcatgctgc ctcgagttcg agaggagctg
     2521 attgacaaga aaactgagaa cgccgtgtct aggatgcagt ctgtgatcga gcttggaaga
     2581 gtgatacgag accgaaaaac tattccaata aagtatcctc tgaaggaaat tgtggtcatc
     2641 caccaagacc cagaagctct agaggatatc aggtccttgg agaagtatat cattgaggaa
     2701 ttaaatgttc gaaaagtcac gctgtctact gataaaaaca agtacggtat ccgactaagg
     2761 gctgaaccag atcacatggt ccttgggaag cgcctgaagg gggccttcaa agcggtgatg
     2821 atggccataa agcggctgag caatgaggag ctggagcggt ttcagaagag cgggagcatt
     2881 gttgtggaag gccacgaact gcatgaggaa gacattcgcc tcatgtacac ctttgaccag
     2941 gccacgggag ggaccgcgca gtttgaagca cactctgatg ctcaggctct agttctctta
     3001 gatgtcacac ctgaccagtc aatggtggat gagggcatgg ctcgggaagt catcaatcgc
     3061 atccagaagc ttcgaaagaa gtgcaatctg gttccaacgg atgaaataac agtttactat
     3121 aatgcaaaat ctgaaggaag atacttgaat aatgtcattg aaagccacac tgacttcata
     3181 tttgccacaa tcaaagctcc cttgaagcca tatccagttc caacatcaga taatatcctt
     3241 attcaagagc aaacacagtt aaaggggtcc gagctggaaa tcacgctcac caaaggatcg
     3301 tgtgtgcctg gccctgcttg tgcttatgtg aaccttaaca tttgtgcaaa tggcaccgag
     3361 caaggtggag tgttgctcct ggagaatccg aaaggtgata atcagctgaa cctggtgaaa
     3421 ctgaagactg ttgttaccag cgtatttggt gttaaaaatg caaagctgtc agtcttccac
     3481 ggtgaaacag aaatacagaa ccaaactgac ttgctgagcc tcagtgggag gacgctgtgt
     3541 gtgactgcag gggcatcccc gtctcccatc agcagcccca gcaccctgct gtgtcagtac
     3601 ctcaacctgc agctcctgaa cgcagagcca caagagtgtt taacaggaac tgtgggcacc
     3661 ctcctgcttg aaaacccact cggacagaac ggcctcaccc accaagggct tgtgcatgaa
     3721 gcagccaagg tgttcggact gaggagcagg aggctaaggc tgttcctgaa tgagactcaa
     3781 acacaagaaa ttacagaaga tatcccaatg aagactttga atatgaagac tgtatatgtt
     3841 tctgtcttgc caacaactgc agatggctaa cagctatcag tatgccttca gtcagtcttc
     3901 cttgaatcta cccatctcct tatacacaca ctcacacact cacactctct atgtctctct
     3961 ctctctctct ctctctctgt ctctctctca cacacacata cacacacaca catgggttca
     4021 gtgaagttat ctccttgggc acttgtcaaa tatgctgctt ttaaccggcg attctaatgg
     4081 gattgactga ctggagacct cacatcatgt tcacatttaa ccttaaacac ctatgcagtc
     4141 atgttggggg agtgtcccaa tgttacctca acaccacgaa gcttatttct atactggagt
     4201 tcttcagtat agaaagtgga agtggtttaa atggcatagt acatatacat atatatatgg
     4261 ccttgtaaaa cagatttgag accttgtgaa gaaatggata tgttatatat ttctgctacc
     4321 tggattttcc tgggtaattt gatggaatat tttacgtttg attaaatcaa aacagtaaac
     4381 actctcttag atactg
//
