LOCUS       NM_001411630            4372 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus hypoxia up-regulated 1 (Hyou1), transcript variant 2,
            mRNA.
ACCESSION   NM_001411630
VERSION     NM_001411630.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Mann,M.J., Melendez-Suchi,C., Vorndran,H.E., Sukhoplyasova,M.,
            Flory,A.R., Irvine,M.C., Iyer,A.R., Guerriero,C.J., Brodsky,J.L.,
            Hendershot,L.M. and Buck,T.M.
  TITLE     Loss of Grp170 results in catastrophic disruption of endoplasmic
            reticulum function
  JOURNAL   Mol Biol Cell 35 (4), ar59 (2024)
   PUBMED   38446639
  REMARK    GeneRIF: Loss of Grp170 results in catastrophic disruption of
            endoplasmic reticulum function.
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Wang,Z., Tan,C., Duan,C., Wu,J., Zhou,D., Hou,L., Qian,W., Han,C.
            and Hou,X.
  TITLE     FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells
            against inflammatory injury by regulating unfolded protein response
  JOURNAL   Redox Biol 60, 102618 (2023)
   PUBMED   36724577
  REMARK    GeneRIF: FUT2-dependent fucosylation of HYOU1 protects intestinal
            stem cells against inflammatory injury by regulating unfolded
            protein response.
REFERENCE   3  (bases 1 to 4372)
  AUTHORS   Sallais,J., Park,C., Alahari,S., Porter,T., Liu,R., Kurt,M.,
            Farrell,A., Post,M. and Caniggia,I.
  TITLE     HIF1 inhibitor acriflavine rescues early-onset preeclampsia
            phenotype in mice lacking placental prolyl hydroxylase domain
            protein 2
  JOURNAL   JCI Insight 7 (23), e158908 (2022)
   PUBMED   36227697
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4372)
  AUTHORS   Porter,A.W., Nguyen,D.N., Clayton,D.R., Ruiz,W.G., Mutchler,S.M.,
            Ray,E.C., Marciszyn,A.L., Nkashama,L.J., Subramanya,A.R.,
            Gingras,S., Kleyman,T.R., Apodaca,G., Hendershot,L.M., Brodsky,J.L.
            and Buck,T.M.
  TITLE     The molecular chaperone GRP170 protects against ER stress and acute
            kidney injury in mice
  JOURNAL   JCI Insight 7 (5), e151869 (2022)
   PUBMED   35104250
  REMARK    GeneRIF: The molecular chaperone GRP170 protects against ER stress
            and acute kidney injury in mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4372)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4372)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   7  (bases 1 to 4372)
  AUTHORS   Tamatani,M., Matsuyama,T., Yamaguchi,A., Mitsuda,N., Tsukamoto,Y.,
            Taniguchi,M., Che,Y.H., Ozawa,K., Hori,O., Nishimura,H.,
            Yamashita,A., Okabe,M., Yanagi,H., Stern,D.M., Ogawa,S. and
            Tohyama,M.
  TITLE     ORP150 protects against hypoxia/ischemia-induced neuronal death
  JOURNAL   Nat Med 7 (3), 317-323 (2001)
   PUBMED   11231630
REFERENCE   8  (bases 1 to 4372)
  AUTHORS   Matsushita,K., Matsuyama,T., Nishimura,H., Takaoka,T., Kuwabara,K.,
            Tsukamoto,Y., Sugita,M. and Ogawa,S.
  TITLE     Marked, sustained expression of a novel 150-kDa oxygen-regulated
            stress protein, in severely ischemic mouse neurons
  JOURNAL   Brain Res Mol Brain Res 60 (1), 98-106 (1998)
   PUBMED   9748521
REFERENCE   9  (bases 1 to 4372)
  AUTHORS   Ikeda,J., Kaneda,S., Kuwabara,K., Ogawa,S., Kobayashi,T.,
            Matsumoto,M., Yura,T. and Yanagi,H.
  TITLE     Cloning and expression of cDNA encoding the human 150 kDa
            oxygen-regulated protein, ORP150
  JOURNAL   Biochem Biophys Res Commun 230 (1), 94-99 (1997)
   PUBMED   9020069
REFERENCE   10 (bases 1 to 4372)
  AUTHORS   Naved,A.F., Ozawa,M., Yu,S., Miyauchi,T., Muramatsu,H. and
            Muramatsu,T.
  TITLE     CBP-140, a novel endoplasmic reticulum resident Ca(2+)-binding
            protein with a carboxy-terminal NDEL sequence showed partial
            homology with 70-kDa heat shock protein (hsp70)
  JOURNAL   Cell Struct Funct 20 (2), 133-141 (1995)
   PUBMED   7641295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122428.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253013.2957535.1,
                                           SRR17253011.6869195.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AC122428.4         105180-105232       c
            54-151              AC122428.4         104165-104262       c
            152-245             AC122428.4         103866-103959       c
            246-324             AC122428.4         103643-103721       c
            325-479             AC122428.4         103367-103521       c
            480-556             AC122428.4         103185-103261       c
            557-738             AC122428.4         102752-102933       c
            739-854             AC122428.4         102207-102322       c
            855-1047            AC122428.4         100474-100666       c
            1048-1182           AC122428.4         100204-100338       c
            1183-1265           AC122428.4         100031-100113       c
            1266-1398           AC122428.4         99813-99945         c
            1399-1586           AC122428.4         99470-99657         c
            1587-1725           AC122428.4         99070-99208         c
            1726-1786           AC122428.4         97683-97743         c
            1787-1866           AC122428.4         97446-97525         c
            1867-2052           AC122428.4         96902-97087         c
            2053-2222           AC122428.4         96597-96766         c
            2223-2310           AC122428.4         96410-96497         c
            2311-2433           AC122428.4         96171-96293         c
            2434-2567           AC122428.4         95850-95983         c
            2568-2652           AC122428.4         95653-95737         c
            2653-2893           AC122428.4         95304-95544         c
            2894-2944           AC122428.4         95148-95198         c
            2945-2995           AC122428.4         93889-93939         c
            2996-4372           AC122428.4         92410-93786         c
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 24.84 cM"
     gene            1..4372
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /note="hypoxia up-regulated 1"
                     /db_xref="GeneID:12282"
                     /db_xref="MGI:MGI:108030"
     exon            1..53
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            54..151
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..3057
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; calcium binding protein 140; 170 kDa glucose
                     regulated protein GRP170; hypoxia up-regulated protein 1;
                     GRP-170; 140 kDa Ca(2+)-binding protein; calcium binding
                     protein, 140 kDa; 150-kDa oxygen regulated protein"
                     /codon_start=1
                     /product="hypoxia up-regulated protein 1 isoform 2
                     precursor"
                     /protein_id="NP_001398559.1"
                     /db_xref="GeneID:12282"
                     /db_xref="MGI:MGI:108030"
                     /translation="MAATVRRQRPRRLLCWALVAVLLADLLALSDTLAVMSVDLGSES
                     MKVAIVKPGVPMEIVLNKESRRKTPVTVTLKENERFLGDSAAGMAIKNPKATLRYFQH
                     LLGKQADNPHVALYRSRFPEHELIVDPQRQTVRFQISPQLQFSPEEVLGMVLNYSRSL
                     AEDFAEQPIKDAVITVPAFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRR
                     KDINSTAQNVMFYDMGSGSTVCTIVTYQTVKTKEAGMQPQLQIRGVGFDRTLGGLEME
                     LRLREHLAKLFNEQRKGQKAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLM
                     DDVDFKAKVTRVEFEELCADLFDRVPGPVQQALQSAEMSLDQIEQVILVGGATRVPKV
                     QEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVIYPILVEFT
                     REVEEEPGLRSLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDL
                     RVFGSQNLTTVKLKGVGESFKKYPDYESKGIKAHFNLDESGVLSLDRVESVFETLVED
                     SPEEESTLTKLGNTISSLFGGGTSSDAKENGTDAVQEEEESPAEGSKDEPAEQGELKE
                     EAEPPAEETSQPPPSEPKGDAAREGEKPDEKESGDKPEAQPNEKGQAGPEGAAPAPEE
                     DKKPKPARKQKMVEEIGVELAVLDLPDLPEDELARSVQKLEELTLRDLEKQEREKAAN
                     SLEAFIFETQDKLYQPEYQEVSTEEQREEISGKLSATSTWLEDEGFGATTVMLKDKLA
                     ELRKLCQGLFFRVEERRKWPERLSALDNLLNHSSIFLKGARLIPEMDQVFTEVEMTTL
                     EKVINDTWAWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAKFTKPR
                     PRPKDKNGTRAEPPLNASAGDQEEKVIPPAGQTEEAKPILEPDKEETGTEPADSEPLE
                     LGGPGAGPEQEEQSAGQKRPSKNDEL"
     exon            152..245
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            246..324
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            325..479
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            480..556
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            557..738
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            739..854
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1047
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1182
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1265
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1398
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1586
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1725
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1786
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..1866
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..2052
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2222
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2310
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2433
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2434..2567
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2568..2652
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2653..2893
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..2944
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..2995
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..4372
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4349..4354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /note="hexamer: AATAAA"
     polyA_site      4372
                     /gene="Hyou1"
                     /gene_synonym="Cab140; CBP-140; Grp170; Orp150"
                     /note="major polyA site"
ORIGIN      
        1 aatgagcgtg cgccgcgtcc ggggccggct ggtgcgcgag acgccgctga gagggtcaca
       61 atggcagcca cagtaagaag gcagaggcca aggaggctac tctgttgggc cttggtggct
      121 gtcctcttgg cagacctgtt ggcactgagc gacacattgg ctgtgatgtc tgtagacctg
      181 ggcagtgaat ccatgaaggt ggccattgtc aagcctggag tgcccatgga gattgtattg
      241 aacaaggaat ctcggaggaa aactccagtg actgtgacct tgaaagaaaa tgaaaggttt
      301 ttaggtgata gtgcagccgg catggccatc aagaacccaa aggctacgct ccgttatttc
      361 cagcacctcc ttggaaaaca ggcggataac cctcatgtgg ccctttaccg gtcccgtttc
      421 ccagaacatg agctaattgt tgacccacag aggcagactg tgcgcttcca gatcagtccg
      481 cagctgcagt tctctcccga ggaggtactg ggcatggttc tgaactactc ccgttccttg
      541 gctgaagatt ttgctgaaca acccattaag gatgcagtga tcaccgtgcc agcctttttc
      601 aaccaggctg agcgccgagc tgtgctgcag gctgctcgga tggctggcct caaggtgctg
      661 cagctcatca atgacaacac tgccacagcc ctcagctacg gtgtcttccg ccggaaagat
      721 atcaattcca ctgcacagaa cgtcatgttc tatgacatgg gctcgggcag cactgtgtgc
      781 accatcgtca cctaccagac agtgaagact aaggaggctg ggatgcaacc acagctgcag
      841 atccggggcg tgggatttga ccgcaccctg ggtggcctgg agatggagct tcggcttcga
      901 gaacacctgg ctaagctctt caatgagcag cgcaagggcc agaaagccaa ggatgttcgg
      961 gaaaaccccc gggccatggc caaactgctt cgggaagcca accggcttaa aaccgtcctg
     1021 agtgccaacg ctgatcacat ggcacagatt gagggcttga tggatgatgt ggacttcaag
     1081 gccaaagtaa ctcgggtgga attcgaggag ctgtgtgcag atttgtttga ccgtgtgcct
     1141 ggacctgtgc agcaggcctt gcagagtgca gagatgagct tggatcaaat tgagcaggtg
     1201 atcctggtgg gcggggccac tcgtgttccc aaagttcaag aagtgctgct caaggccgtg
     1261 ggcaaggagg aactaggaaa gaacatcaat gcggacgaag ctgctgccat gggggctgtg
     1321 taccaggcag cggcgctcag caaggccttc aaagtgaagc catttgttgt gcgggatgct
     1381 gtcatttacc caatcctggt ggagttcaca agggaggtgg aggaggagcc tgggctccga
     1441 agcctgaaac acaataagcg tgtgctcttc tcccgaatgg ggccctaccc tcagcgcaaa
     1501 gtcatcacct ttaaccgcta cagccatgat ttcaacttcc acatcaacta cggtgacctg
     1561 ggcttcctgg ggcctgagga tcttcgggta tttggctccc agaatctgac cacagtaaaa
     1621 ctaaaaggcg tgggagagag cttcaagaaa tatcccgact atgagtccaa aggcatcaag
     1681 gcccacttta acctggatga gagtggcgtg ctcagtttag acagggtgga gtccgtattt
     1741 gagaccctgg tggaggatag cccagaggaa gaatctactc ttaccaaact tggcaacacc
     1801 atatccagcc tgtttggagg tggtacctca tcagatgcca aagagaatgg tactgatgct
     1861 gtacaggagg aagaggagag ccccgctgag gggagcaagg atgagcctgc agagcagggg
     1921 gaactcaagg aagaagctga acccccagca gaggagacct ctcagcctcc accctctgag
     1981 cctaaggggg atgcagcccg tgagggagag aaacctgatg aaaaagagag tggggacaag
     2041 cctgaggccc agcccaatga gaaggggcaa gcagggcctg agggtgctgc tccagctcct
     2101 gaggaggaca aaaagccgaa acctgcccgg aagcagaaaa tggtggagga gataggtgtg
     2161 gagctggctg tcttggacct gcctgacttg ccagaggatg agctggcccg ttctgtgcag
     2221 aaacttgaag aactgaccct gcgcgaccta gagaagcagg agagggagaa agctgccaac
     2281 agcttggagg ctttcatctt tgagacccag gacaagctgt accagcctga gtaccaggaa
     2341 gtgtccactg aggaacagcg ggaggagatc tcggggaaac tcagcgccac ttctacctgg
     2401 ctggaggatg agggatttgg agccaccact gtgatgctga aggacaagct ggctgagctg
     2461 agaaagctgt gccaagggct gttttttcgg gtggaagaac gcaggaaatg gccagagcgg
     2521 ctttcagctc tggataatct cctcaaccat tccagcattt tcctcaaggg tgcccggctc
     2581 atcccggaga tggaccaggt cttcactgaa gtggagatga cgacattaga gaaagtcatc
     2641 aatgacacct gggcctggaa gaatgcaact ctggccgaac aagccaagct tcctgccaca
     2701 gagaagcctg tgctgctttc aaaagacatt gaggccaaaa tgatggccct ggaccgggag
     2761 gtacagtatc tactcaataa ggccaagttt accaagccac ggccacggcc caaagacaag
     2821 aatggcaccc gggcagaacc tcccctcaat gccagtgctg gtgaccaaga ggagaaggtc
     2881 attccacctg caggccagac tgaagaggcg aaacccattt tagaacctga caaagaagag
     2941 actggtacgg aaccagcaga ctcggagcct ctggaattag gaggtcctgg agctggacct
     3001 gaacaggaag agcagtcagc aggacagaag cggccttcaa agaacgatga actataaccc
     3061 cagctcctgc cttccgactt ggctccagcc ccttctccta ccacctctat ttattataca
     3121 tcagggttgg agtgggggtt ggtccctcag gggctcaagt tccttctctc agctgggaca
     3181 ggaggtggct gctcagtcct agagtagctc tatgggtgaa accggctcta gttctgtccc
     3241 cagccccacc ctctggtctc ctactctctg gccctgtgtt tgggaaaaat cactattatg
     3301 tcttaattct ttgcctgtgg gtaagtgaga gaatggctgc cagtagttgt tggagacctg
     3361 gtagtgggaa ggatgttatg ggtcactgga aagtggccag tcttcacagc atcctttcat
     3421 cttttcctct tccacaaaat cagtgtcctg tttgggccag tgtctacaga gcctcagtcc
     3481 cacttggttt ctgagtgcct cttttctctt ctgtgtcttc tcttcccttg tccttcctcc
     3541 ttggctcttc tgtcctttcc ttccctgtgc agacaggact atggtcttct tgcccaaaga
     3601 ctttcatctg gactgtctat agagtaaata tgctctcatg gttcatgcga cccctggtgg
     3661 ctggagtaag cggggagcag gcaagaaggg aaagccctgt agccctgaat gggttgttgg
     3721 acccaggaca gtacaggtcc ggagagtggc tggcaaggat gcaagtatac tgcaggcact
     3781 cgacatcctt gtcagcccca gttcctgtga cacctacccc ttcatgctct ttcctccttc
     3841 cccctgggct gaccaaaaat gtgctatcta ctgtgagccc ctttcccaag accctggggg
     3901 acggagagac catggagtga atgtaaaaat gccacctcac tctcaggcaa gctttgtggg
     3961 taaagacgag gggtcagggt tcaacttgac agtgtacttc gctaggaagg agaggccctt
     4021 gcctgctata accatgccta gagtgcctgt cctccagtat tgcacctgta gccggagcta
     4081 gggccacccc agtgcagggg caaagcaaac ctgatgtcag tcgttttgtc ttttctaccc
     4141 caacctcacc ctctcaccct actttccacc ccccaccccg cctttttttt tttttttttt
     4201 ttttttttgt gccacattgg cagaggggcc tataggtcct tcactgttcc ctcctgtgtg
     4261 ttggagttct ttagttagca gttcaggctg gttggacagg tttgaatcaa attgtacttt
     4321 gttccattgt taattgagaa actgtttcaa taaaatattc ttttctatag gt
//
