LOCUS       NM_028920               3452 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus hyaluronoglucosaminidase 6 (Hyal6), mRNA.
ACCESSION   NM_028920 XM_133003
VERSION     NM_028920.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Lu,Y., Oura,S., Matsumura,T., Oji,A., Sakurai,N., Fujihara,Y.,
            Shimada,K., Miyata,H., Tobita,T., Noda,T., Castaneda,J.M.,
            Kiyozumi,D., Zhang,Q., Larasati,T., Young,S.A.M., Kodani,M.,
            Huddleston,C.A., Robertson,M.J., Coarfa,C., Isotani,A.,
            Aitken,R.J., Okabe,M., Matzuk,M.M., Garcia,T.X. and Ikawa,M.
  TITLE     CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched
            genes dispensable for male fertility in micedagger
  JOURNAL   Biol Reprod 101 (2), 501-511 (2019)
   PUBMED   31201419
REFERENCE   3  (bases 1 to 3452)
  AUTHORS   Reitinger,S., Laschober,G.T., Fehrer,C., Greiderer,B. and
            Lepperdinger,G.
  TITLE     Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but
            not HYALP1 degrade hyaluronan
  JOURNAL   Biochem J 401 (1), 79-85 (2007)
   PUBMED   16925524
  REMARK    GeneRIF: Mouse SPAM1 and HYAL5 but not HYALP1 proteins exhibit
            hyaluronidase activity at neutral pH.
REFERENCE   4  (bases 1 to 3452)
  AUTHORS   Miller,K.A., Shao,M. and Martin-DeLeon,P.A.
  TITLE     Hyalp1 in murine sperm function: evidence for unique and
            overlapping functions with other reproductive hyaluronidases
  JOURNAL   J Androl 28 (1), 67-76 (2007)
   PUBMED   16928892
  REMARK    GeneRIF: findings reveal that Hyalp1 likely has a unique function
            in the adult testis, and redundant overlapping ones with Spam1 and
            may compensate for it in Spam1 null mice
REFERENCE   5  (bases 1 to 3452)
  AUTHORS   Zhang,H., Shertok,S., Miller,K., Taylor,L. and Martin-Deleon,P.A.
  TITLE     Sperm dysfunction in the Rb(6.16)- and Rb(6.15)-bearing mice
            revisited: involvement of Hyalp1 and Hyal5
  JOURNAL   Mol Reprod Dev 72 (3), 404-410 (2005)
   PUBMED   16078272
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK016575.1 and AC130215.3.
            
            On Feb 14, 2007 this sequence version replaced NM_028920.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029715.1, AK016575.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2580              AK016575.1         2-2581
            2581-3452           AC130215.3         95218-96089
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 11.28 cM"
     gene            1..3452
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /note="hyaluronoglucosaminidase 6"
                     /db_xref="GeneID:74409"
                     /db_xref="MGI:MGI:1921659"
     exon            1..275
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /inference="alignment:Splign:2.1.0"
     exon            276..1230
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             307..1791
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /EC_number="3.2.1.35"
                     /note="hyaluronoglucosaminidase, pseudogene 1"
                     /codon_start=1
                     /product="hyaluronoglucosaminidase 6 precursor"
                     /protein_id="NP_083196.2"
                     /db_xref="CCDS:CCDS51728.1"
                     /db_xref="GeneID:74409"
                     /db_xref="MGI:MGI:1921659"
                     /translation="MFIQWVTQLGELVLFVLLVAPAALKPAMPPVIKDHPFNVFWAAP
                     TLFCKDNFNVNMNLQVFNIIPNPFETQSGSTITVFYPKELGYYPYFSEDGTSFYGGIP
                     QKVNLSEHLRKSAGDIADAVTLWRSEGLAVIDWEGWRPQWDRNWGSRMIYKNHSLAFT
                     RHHHPDWAETKVRTAAQKEFENAGRSFMNVTLTLALEMRPKRLWGFYLYPDCYNYDYR
                     INPEFYTGSCPDDEIFRNDQLMWLWEKSAALYPSIYLSKILKSNLNALKFVHFRVREA
                     LRVAEMARKDYVLPVFIFSRPFYLQSIEALSEEDLVHTIGESAALGAAGIILWGGYEY
                     SDTKETCLSVRQTVHGLLGPYVLNVTSAAKLCSQNLCNSHGRCVRKTPESSFYLHMPE
                     DSHKNYVSKKGFRFVIPSPSKLKTIMNMKNGFVCHCYYGWHGDSCRSHSPNLQKNKAP
                     ASGLNSAVIVGMALFVILMNYFPIPYYNGNFSLKPLKRREIIFL"
     exon            1231..1320
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1321..3452
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2656..2661
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /note="hexamer: AATAAT"
     polyA_site      2697
                     /gene="Hyal6"
                     /gene_synonym="4932701A20Rik; Hyal-ps1; Hyalp1"
                     /note="major polyA site"
ORIGIN      
        1 ggcagttttt ttgtcttaac tgactatgtg tgactaaaag gccgagtttt acctgcaacc
       61 cagcgtctga gaatatggcc ccatccatcc cttcatagaa gagtagtggt cttaaggaaa
      121 taatagtatt aaaatgttta tttctatgcc caagaaaaga cattagcaaa atctacagtc
      181 aacaacagat gattggcagc acctctattt cctgtgtcat gatattcccg tgggaaccct
      241 tctgtgtttt ggaaacaatc aaataaacct ttgagcacac ttccggcatg ctgaaaacag
      301 caatccatgt ttatccagtg ggtgacacag cttggtgaac tggtgctctt cgtccttctg
      361 gtcgctccgg cagctctgaa acccgcaatg cctcctgtga tcaaggacca tcctttcaac
      421 gttttctggg ccgcccctac tttgttttgt aaagataatt tcaatgtaaa catgaatctt
      481 caagtattta acatcattcc gaatcctttc gaaactcaga gtggatccac tattaccgta
      541 ttttatccaa aggaactcgg gtattaccct tatttctctg aagatggaac atccttctat
      601 ggtggaatac ctcagaaagt gaacctctct gagcacctca ggaaaagtgc cggcgacatt
      661 gcagatgctg tcactttgtg gagatcggaa ggacttgctg ttatagactg ggaaggctgg
      721 aggccccagt gggataggaa ttggggcagt agaatgatat ataaaaacca ctcgctagcc
      781 ttcactcgac accaccatcc tgactgggca gaaacaaaag tgagaacagc tgcccagaaa
      841 gaatttgaaa atgctggaag aagttttatg aatgttactc tcacgttggc tttagaaatg
      901 agaccaaagc gtttatgggg cttctatctc tatccagatt gctacaatta tgattaccgg
      961 ataaatccag agttctacac aggaagttgc ccagatgatg aaattttccg caatgaccaa
     1021 ctcatgtggc tatgggaaaa aagtgcagca ctttatcctt ccatatattt gagtaaaata
     1081 ttaaaatcaa acttaaatgc attgaaattt gttcatttcc gagtgagaga agccctgagg
     1141 gttgccgaaa tggctagaaa ggactatgtt ttgccagttt tcattttttc cagaccattt
     1201 tatttgcaaa gtattgaagc tctctcagag gaggatttag tgcacacaat tggcgaaagt
     1261 gctgcattgg gagcagcagg aataatattg tggggaggat atgaatattc ggatacaaag
     1321 gagacttgct tatctgtgag acaaactgtt catgggctat tgggtcccta tgttctcaat
     1381 gtaacatcgg cagcaaagct ctgtagtcaa aatctatgta acagtcatgg aagatgtgta
     1441 cggaagacac ctgaatcttc cttttatctg catatgcctg aggatagcca taagaattat
     1501 gtgtcaaaga agggtttcag atttgtcatt ccctccccaa gtaagctgaa gacaataatg
     1561 aatatgaaga atggatttgt gtgccactgt tactatggct ggcatggaga ctcctgcaga
     1621 tctcactctc caaatctcca aaagaacaaa gctcctgcaa gtggattgaa ttcagcagtt
     1681 attgttggca tggccttatt tgtgattctg atgaattatt ttcctattcc ctactacaat
     1741 ggcaattttt ccttgaaacc actgaagaga agagaaatta tctttctttg atttcaagca
     1801 ttttctaaag aatttttagt tttaagtttc atacaaattg ttggatgttc acttcatgct
     1861 gggcaatagg aatagacctg tcctcgtgga tcttagaaat taagaataaa taagcagtaa
     1921 atagacacat atgatagtga caatggtttg tagaataaag agaaaacagg tttaaggcat
     1981 taggaagtac tagagagcac tggaaattgt ataaaagatt gtcacataag gcctttatag
     2041 tgagaaaatc agataacaga tgaattaggt caacactatc agggagcgag atattagttt
     2101 cctcctataa tctgagcacc tggaaggcag agatccatta caaattcaaa gccagcttgg
     2161 cttacaaact gagctaaagt cagtcaagac aacatagtga aagttttgtc ttcaaaaaag
     2221 gaaaatagaa gcaaaatagc aaagatccaa aagaacgaat ctggggaaag agttcttgaa
     2281 ataaaaagga gcaaacccag aagagacaac tctgcatttc agggcttgta aacttttaaa
     2341 taacaagcaa cacaacaata gcaaaacaac agcagcagca gcagcaacaa tgatactcac
     2401 cgatggagat gaaaatgact cttccaatgg tttcatcaat gattagcttt atccagcgga
     2461 catttggttc atcaatacca aaagagaact ctaccagctt cctacacaat aggatttatg
     2521 tcagaaattc ttttgtttga tactctttgg tttattgaca tatactatgc ccaaacagtc
     2581 tgtcaattgt cactggatgg taattcattt gaaaattttc attatggtga cctgacttaa
     2641 cctagctgta ttccaaataa ttttatttca caaagacaca ttttcacaaa gactcaatcg
     2701 agtgagtgtg gtgtcctaaa tattattttc taaggcaagg aagggtattt ccctgtctag
     2761 tatgtcccaa actctgcagt attaggtaga tatcatcctg gagcacacac agaactttat
     2821 aaaacctcat attcagattc ttatactaac attgtttcat taattagtta ttccaccatg
     2881 tattctggga aaaagtaaat agtaaagaag cagtatcgga agctagattg atgggcatgt
     2941 agaagttctc ctgttttgtt ggtctccagt agcacaaaac ctataggtac aggaactaaa
     3001 gcctccggca aaagcatgca atgtttttcg taagtattca attgcctgct atgtcataaa
     3061 gaagcaatga ctcctcctaa tcttccagtg atgaaaactt acttaacaac tattatatca
     3121 gggctcagac actgaactac tcctagtaaa gcaggaaagg cagatattaa gtattaatac
     3181 aatcttgaag gtgaaggaaa cgaagcaggt aaagaataat acatgctcag ttgtgttcag
     3241 ccaccagtga ataatacagc aatggtagaa cccactgttt aaactgcccc tgaaataatt
     3301 aggaaagatg aaagtattgg tctattaggg agaaagggga aaataattag taaaagaaat
     3361 gctagtaacc ctagataggc atttgcaatt ttataaatac aaacactatt aagttttact
     3421 tcactagtat tataaaataa aaacaatacc ac
//
