LOCUS       NM_028957               2090 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus hyaluronoglucosaminidase 5 (Hyal5), transcript variant
            1, mRNA.
ACCESSION   NM_028957 XM_355747
VERSION     NM_028957.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Park,S., Kim,Y.H., Jeong,P.S., Park,C., Lee,J.W., Kim,J.S., Wee,G.,
            Song,B.S., Park,B.J., Kim,S.H., Sim,B.W., Kim,S.U.,
            Triggs-Raine,B., Baba,T., Lee,S.R. and Kim,E.
  TITLE     SPAM1/HYAL5 double deficiency in male mice leads to severe male
            subfertility caused by a cumulus-oocyte complex penetration defect
  JOURNAL   FASEB J 33 (12), 14440-14449 (2019)
   PUBMED   31670981
  REMARK    GeneRIF: A comparative analysis between WT and Spam1/Hyal5 dKO
            epididymal sperm revealed that the absence of these 2 sperm Hyals
            resulted in a marked accumulation of sperm on the outside of the
            cumulus-oocyte complex.
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Kang,W., Zhou,C., Koga,Y. and Baba,T.
  TITLE     Hyaluronan-degrading activity of mouse sperm hyaluronidase is not
            required for fertilization?
  JOURNAL   J Reprod Dev 56 (1), 140-144 (2010)
   PUBMED   19926939
  REMARK    GeneRIF: The data from this paper suggests that the
            hyaluronan-degrading activity of the sperm hyaluronidase HYAL5 may
            not be required for fertilization, at least in the mouse.
REFERENCE   3  (bases 1 to 2090)
  AUTHORS   Kimura,M., Kim,E., Kang,W., Yamashita,M., Saigo,M., Yamazaki,T.,
            Nakanishi,T., Kashiwabara,S. and Baba,T.
  TITLE     Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in
            fertilization
  JOURNAL   Biol Reprod 81 (5), 939-947 (2009)
   PUBMED   19605784
  REMARK    GeneRIF: The HYAL5-deficient female mice were fully fertile and
            yielded normal litter sizes. HYAL5-deficient epididymal sperm is
            functionally normal. Thus, HYAL5 may be dispensable for
            fertilization.
REFERENCE   4  (bases 1 to 2090)
  AUTHORS   Reitinger,S., Laschober,G.T., Fehrer,C., Greiderer,B. and
            Lepperdinger,G.
  TITLE     Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but
            not HYALP1 degrade hyaluronan
  JOURNAL   Biochem J 401 (1), 79-85 (2007)
   PUBMED   16925524
  REMARK    GeneRIF: Mouse SPAM1 and HYAL5 but not HYALP1 proteins exhibit
            hyaluronidase activity at neutral pH.
REFERENCE   5  (bases 1 to 2090)
  AUTHORS   Kim,E., Baba,D., Kimura,M., Yamashita,M., Kashiwabara,S. and
            Baba,T.
  TITLE     Identification of a hyaluronidase, Hyal5, involved in penetration
            of mouse sperm through cumulus mass
  JOURNAL   Proc Natl Acad Sci U S A 102 (50), 18028-18033 (2005)
   PUBMED   16330764
REFERENCE   6  (bases 1 to 2090)
  AUTHORS   Zhang,H., Shertok,S., Miller,K., Taylor,L. and Martin-Deleon,P.A.
  TITLE     Sperm dysfunction in the Rb(6.16)- and Rb(6.15)-bearing mice
            revisited: involvement of Hyalp1 and Hyal5
  JOURNAL   Mol Reprod Dev 72 (3), 404-410 (2005)
   PUBMED   16078272
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127559.6.
            
            On Mar 10, 2023 this sequence version replaced NM_028957.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017112.1, AK133387.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               AC127559.6         74324-74452         c
            130-1268            AC127559.6         55352-56490         c
            1269-1358           AC127559.6         54485-54574         c
            1359-2090           AC127559.6         40471-41202         c
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 11.28 cM"
     gene            1..2090
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /note="hyaluronoglucosaminidase 5"
                     /db_xref="GeneID:74468"
                     /db_xref="MGI:MGI:1921718"
     exon            1..129
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            130..1268
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             315..1895
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /EC_number="3.2.1.35"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; hyaluronidase Hyal-5; hyal-5; hyaluronidase
                     PH-20; hyaluronidase-5"
                     /codon_start=1
                     /product="hyaluronidase-5 isoform a precursor"
                     /protein_id="NP_083233.1"
                     /db_xref="CCDS:CCDS19948.1"
                     /db_xref="GeneID:74468"
                     /db_xref="MGI:MGI:1921718"
                     /translation="MRVLYFKHSFFRSLLKSNGLPQTLLVFLLIPCYLTVDFRAPPLI
                     PDVPFLWAWNAPTESCFTRFNQPLDLGLFSLVGSPRKSATGQPVTIFYSDRLGLYPYI
                     DDSQLIFNGGLPQLVSLKSHLEVAKTDILHYMPIDNVGLAVIDWEEWRPTWARNWKPK
                     DIYRNKSIELVQQQNILLNFTEAVKWAKEEFEEAARHFMEETLRLGKSLRPNHLWGFY
                     LFPDCYNNKFQVADYKGECPDIEKHRNDALFWIWEESTALYPSIYLKSSLKSSPQAAL
                     YVRNRVQEAIRVSKVKDPRNPLPIFVYFRIVFTDLTYQYLYEDDLVNTIGETIALGTS
                     GMVMWGTLSLSQTMKSCLDLHDYLKTILNPYIINVTLAAKMCSQTLCQNQGVCSRKDW
                     NSNDYLHLNPQNFQIHFVKHGKYEIRGNPTLENLLYFSQKFRCSCFAHLNCQERADIE
                     SVSTVSVCTLEDICINSLVISDKSELPKDWNRPYFVNSNQSDITSSATVSPCVPRKDV
                     SGYLVVLSLYSQHLKYSL"
     exon            1269..1358
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..2090
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1983..1988
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /note="hexamer: AATATA"
     polyA_site      2007
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
     regulatory      2069..2074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2090
                     /gene="Hyal5"
                     /gene_synonym="4933439A12Rik"
                     /note="major polyA site"
ORIGIN      
        1 attccatctg ttctgggtat gttggtgagt ctagctaggc catggttcac atgaacacag
       61 gaggagtatt ttttactata atgaaagtgc aggtggtgag gagttctgca taaggtcttt
      121 tccagcaagt catatattat acaacttgtc acaaaacatt cctgaagtat gaagcaagaa
      181 gaacaatagt tcaatgtaaa gcagtcatca aactttcacc tcccacagtt ttttctcacc
      241 tgttgcacca aatatttgag agaccaaagt gtgtaagaag aaataagcaa ttggatacta
      301 attaattctc tgcaatgaga gtgttgtatt ttaaacactc cttttttcgg agccttctta
      361 agtctaatgg actaccccag acactattag tcttccttct gattccgtgc tacttgactg
      421 tggattttag ggcaccccca cttatccctg atgtgccttt cctctgggcc tggaatgccc
      481 caactgagtc ttgtttcaca aggtttaatc agccactaga tcttggtctg ttctctttag
      541 taggaagccc cagaaaaagt gccacgggtc aacctgtcac aatattttat agtgatcgtc
      601 tgggtctcta tccttacata gatgactcac aattaatttt taatggaggg ctaccccagc
      661 tggtgtctct aaaatctcat ttggaagtag ctaagactga catcttgcat tacatgccaa
      721 tagacaatgt gggcctggct gtcattgatt gggaagaatg gcggcctacc tgggcaagaa
      781 attggaaacc caaggatatt tataggaata agtctattga gttggtccag caacaaaata
      841 tactacttaa tttcacagaa gctgtcaagt gggccaaaga agagtttgaa gaggcagcaa
      901 gacattttat ggaagagact ttaagattgg gaaagtcact tcggccaaat cacttatggg
      961 gcttttatct ttttcctgat tgttataaca ataaatttca agttgctgat tacaaaggag
     1021 aatgccctga tatagaaaag cacagaaatg atgctctctt ctggatatgg gaggaaagca
     1081 cagccctgta cccatccatt tatttgaaga gtagcttgaa gtcatcccca caagcagcac
     1141 tgtatgtcag aaatcgtgta caagaagcca ttcgagtgtc taaagtcaaa gaccctcgaa
     1201 atccacttcc tatttttgtg tattttcgca ttgtctttac tgacctaact taccaatatc
     1261 tatatgagga tgaccttgtg aatacaattg gtgaaacaat tgctctgggt acctctggaa
     1321 tggtaatgtg gggaactctt agtttatcac aaactatgaa atcttgtctg gacttacatg
     1381 attacttgaa gaccatcctg aatccttata tcatcaacgt cacccttgca gccaaaatgt
     1441 gtagccaaac cctttgccag aatcaaggcg tgtgttcgag gaaggattgg aattctaatg
     1501 actatctcca cttgaaccct cagaactttc agattcattt tgtgaaacat ggaaagtatg
     1561 aaatccgtgg caatcccacc cttgagaacc tgctatactt ttcccaaaaa ttccgttgca
     1621 gctgttttgc ccacctgaac tgtcaggaga gagctgacat agaaagtgtt agcactgtca
     1681 gtgtgtgtac tctggaagac atttgcatca attcccttgt aatttcagac aaaagcgaac
     1741 tacccaagga ctggaatcgc ccttactttg ttaatagcaa tcagtcagat attacatcat
     1801 ctgccacagt gagtccatgt gtgccaagaa aagatgtcag tgggtatctc gttgttctct
     1861 ctctctattc acagcatttg aaatattcgc tatagagttt taaccactca aaaactggac
     1921 accacctttc aaaaataatt ccatctaaat aatcaggatt atactttgaa tttcagaggc
     1981 aaaatatata ttcttatata taagaaattc tgattgtaag gctgtcatac tttcagggtt
     2041 aatggtctac tgagaagagt gaagattaaa taaaatcaag ttgctataaa
//
