LOCUS       NM_001303532            4653 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus HUS1 checkpoint clamp component (Hus1), transcript
            variant 2, mRNA.
ACCESSION   NM_001303532 XM_006514532
VERSION     NM_001303532.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Akagawa,R., Nabeshima,Y.I. and Kawauchi,T.
  TITLE     Alternative Functions of Cell Cycle-Related and DNA Repair Proteins
            in Post-mitotic Neurons
  JOURNAL   Front Cell Dev Biol 9, 753175 (2021)
   PUBMED   34746147
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Hong,H., Yao,S., Zhang,Y., Ye,Y., Li,C., Hu,L., Sun,Y., Huang,H.Y.
            and Ji,H.
  TITLE     In vivo miRNA knockout screening identifies miR-190b as a novel
            tumor suppressor
  JOURNAL   PLoS Genet 16 (11), e1009168 (2020)
   PUBMED   33137086
  REMARK    GeneRIF: In vivo miRNA knockout screening identifies miR-190b as a
            novel tumor suppressor.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4653)
  AUTHORS   Balmus,G., Lim,P.X., Oswald,A., Hume,K.R., Cassano,A., Pierre,J.,
            Hill,A., Huang,W., August,A., Stokol,T., Southard,T. and Weiss,R.S.
  TITLE     HUS1 regulates in vivo responses to genotoxic chemotherapies
  JOURNAL   Oncogene 35 (5), 662-669 (2016)
   PUBMED   25915840
  REMARK    GeneRIF: Whereas Hus1(neo/neo) and Atm(-/-) single mutant mice
            survived low-dose MMC similar to wild-type controls,
            Hus1(neo/neo)Atm(-/-) double mutants showed striking MMC
            hypersensitivity.
REFERENCE   4  (bases 1 to 4653)
  AUTHORS   Wallace,M.D., Southard,T.L., Schimenti,K.J. and Schimenti,J.C.
  TITLE     Role of DNA damage response pathways in preventing carcinogenesis
            caused by intrinsic replication stress
  JOURNAL   Oncogene 33 (28), 3688-3695 (2014)
   PUBMED   23975433
REFERENCE   5  (bases 1 to 4653)
  AUTHORS   Lyndaker,A.M., Lim,P.X., Mleczko,J.M., Diggins,C.E., Holloway,J.K.,
            Holmes,R.J., Kan,R., Schlafer,D.H., Freire,R., Cohen,P.E. and
            Weiss,R.S.
  TITLE     Conditional inactivation of the DNA damage response gene Hus1 in
            mouse testis reveals separable roles for components of the
            RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance
  JOURNAL   PLoS Genet 9 (2), e1003320 (2013)
   PUBMED   23468651
  REMARK    GeneRIF: HUS1 acts as a component of the canonical 9-1-1 complex
            during meiotic prophase I to promote DSB repair and further propose
            that RAD1 and TOPBP1 respond to unsynapsed chromatin through an
            alternative mechanism that does not require RAD9 or HUS1.
REFERENCE   6  (bases 1 to 4653)
  AUTHORS   Weiss,R.S., Enoch,T. and Leder,P.
  TITLE     Inactivation of mouse Hus1 results in genomic instability and
            impaired responses to genotoxic stress
  JOURNAL   Genes Dev 14 (15), 1886-1898 (2000)
   PUBMED   10921903
REFERENCE   7  (bases 1 to 4653)
  AUTHORS   Venclovas,C. and Thelen,M.P.
  TITLE     Structure-based predictions of Rad1, Rad9, Hus1 and Rad17
            participation in sliding clamp and clamp-loading complexes
  JOURNAL   Nucleic Acids Res 28 (13), 2481-2493 (2000)
   PUBMED   10871397
REFERENCE   8  (bases 1 to 4653)
  AUTHORS   Weiss,R.S., Kostrub,C.F., Enoch,T. and Leder,P.
  TITLE     Mouse Hus1, a homolog of the Schizosaccharomyces pombe hus1+ cell
            cycle checkpoint gene
  JOURNAL   Genomics 59 (1), 32-39 (1999)
   PUBMED   10395797
REFERENCE   9  (bases 1 to 4653)
  AUTHORS   Dean,F.B., Lian,L. and O'Donnell,M.
  TITLE     cDNA cloning and gene mapping of human homologs for
            Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of
            homologs from mouse, Caenorhabditis elegans, and Drosophila
            melanogaster
  JOURNAL   Genomics 54 (3), 424-436 (1998)
   PUBMED   9878245
REFERENCE   10 (bases 1 to 4653)
  AUTHORS   Kostrub,C.F., Knudsen,K., Subramani,S. and Enoch,T.
  TITLE     Hus1p, a conserved fission yeast checkpoint protein, interacts with
            Rad1p and is phosphorylated in response to DNA damage
  JOURNAL   EMBO J 17 (7), 2055-2066 (1998)
   PUBMED   9524127
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB611267.1, AK046152.1,
            AL669837.3 and DV650764.1.
            
            On Jan 13, 2015 this sequence version replaced XM_006514532.1.
            
            Summary: This gene encodes a component of a cell cycle checkpoint
            complex that causes cell cycle arrest in response to bulky DNA
            lesions and DNA replication blockage. Together with the proteins
            Rad9 and Rad1, the encoded protein forms a heterotrimeric complex
            known as the 9-1-1 complex. Mice lacking the encoded protein
            develop spontaneous chromosomal abnormalities resulting in
            embryonic lethality. Alternative splicing of this gene results in
            multiple transcript variants. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. It
            encodes isoform 2 which is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046152.1, ERR3363660.2776621.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                BB611267.1         4-57
            55-1559             AK046152.1         2-1506
            1560-1560           AL669837.3         10393-10393         c
            1561-2093           AK046152.1         1508-2040
            2094-4636           AL669837.3         7317-9859           c
            4637-4653           DV650764.1         629-645
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 5.74 cM"
     gene            1..4653
                     /gene="Hus1"
                     /note="HUS1 checkpoint clamp component"
                     /db_xref="GeneID:15574"
                     /db_xref="MGI:MGI:1277962"
     exon            1..133
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..924
                     /gene="Hus1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     checkpoint protein HUS1; Hus1 homolog"
                     /codon_start=1
                     /product="checkpoint protein HUS1 isoform 2"
                     /protein_id="NP_001290461.1"
                     /db_xref="GeneID:15574"
                     /db_xref="MGI:MGI:1277962"
                     /translation="MKFRAKIVDLACLNHFTRVSNMIAKLAKTCTLRISPEKLNFILC
                     DKLASGGVSMWCELEQENFFSEFQMEGVSEENNEIYLELTSENLSRALKTAQNSRALK
                     IKLTNKHFPCLTVSVELVSSSSSSRIVVHDIPIKVLPRRLWKDLQEPSIPDCDVSICL
                     PALKMMKSVVEKMRNISNQLVIEANLKGELNLKIETELVCVTTHFKDLENPLLPSDSV
                     SQNRHPEDMAKVHIDIKKLLQFLAGQQVTPTKAVCNIVNNRTVHFDLLLEDVSLQYFI
                     PALS"
     exon            134..261
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            262..438
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            439..546
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            547..621
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            622..721
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            722..841
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     exon            842..4637
                     /gene="Hus1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4446..4451
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hus1"
                     /note="hexamer: AATAAA"
     polyA_site      4473
                     /gene="Hus1"
     regulatory      4612..4617
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hus1"
                     /note="hexamer: AATAAA"
     polyA_site      4631
                     /gene="Hus1"
                     /note="major polyA site"
ORIGIN      
        1 gcccaggttt ccccgccggg acgctcaggg agggactttt gtccgccttg gaccaacgcc
       61 gccgtcggcc tggtatccgc catgaagttt cgcgccaaga tcgtggacct ggcttgtctg
      121 aatcatttca cacgagtcag taacatgata gccaagcttg ccaaaacctg caccctccgc
      181 atcagcccgg agaagctgaa cttcatcctg tgcgacaagc tggccagtgg aggcgtgagc
      241 atgtggtgtg agctggagca ggagaacttt tttagtgaat ttcaaatgga aggagtctct
      301 gaagaaaaca acgagattta tttagaatta acgtcggaaa acttatctcg agccttgaaa
      361 actgcccaga actccagagc cttgaaaatc aagctgacta acaaacactt tccctgtctt
      421 accgtgtctg tagagctggt gtcttcatcg agcagcagca gaatcgtggt gcatgatatc
      481 cccataaagg ttcttccgag aagactgtgg aaggacttac aagaaccctc catcccagac
      541 tgtgacgtca gtatttgctt accagccttg aagatgatga agagtgttgt ggaaaaaatg
      601 agaaacatca gcaatcagct tgtgattgaa gcaaacctaa agggagaatt aaacctaaag
      661 atagaaactg agttagtgtg tgtgactact cattttaagg atcttgaaaa ccctctatta
      721 ccctctgaca gtgtctctca aaacagacac ccagaagaca tggccaaggt gcacattgac
      781 ataaagaaac tcctccagtt tcttgccgga cagcaagtga ctcccaccaa ggcagtgtgc
      841 aatattgtga ataacagaac tgttcatttt gatttgctcc tggaagacgt ctcccttcag
      901 tatttcatcc cagccttgtc ctagcaccgt ctcagcagac tcaggagcac agagaccttt
      961 gtcactatga gagaagcact ggacgatatg gtcagtcagc tgcctatccg cacatctgta
     1021 cttggcttat ttcgctccct atgctgcttt aagcacaaat ggaattaatg aagcacagtt
     1081 gaatagtcac tccttcaaat tactcaatac caatcccctt atctctttta agacattaag
     1141 aagaaaagaa tgtatttttt atagagtttt tgttgttttg ttttcttctg gtgccggaga
     1201 tgagaccagg gcgtcatgca tactgagcac gcactctgcc actgagctgc tccccacccc
     1261 tatctaaaat ttgagtagca gtttgtcaat gacgttgcaa aaattatatg tgtagaattc
     1321 atgttgcagt cagtacaacc caggtagttt ccagttcgtt gcctggtata acacagctgt
     1381 ctgctttcag agtttagcat gctcatttct cccatccaca catctaggag atcagtgcga
     1441 aacggacagc ccctctgtcc ctgcagcatg ccaggctctc accacactgt tgagtataca
     1501 gctactgcat gtggagcctg tgtgatttac tgaagattcg agtccactca cttccttctc
     1561 gcggcctgca gagccagaga gcctgaggaa cctgcagagc tcccagcctg gagaaactgg
     1621 gtccctgaga gcctaaagga cccacaaacc tacccccagc ccacacagtt cccatcttga
     1681 acttgtcctt tttgtatttt ctggttttag gttataattt aattccctgt tttgtagcac
     1741 tcaggtcaag cccaggactg ggttcatgct aggcaggcac tgtgtgccaa actctatctc
     1801 cagtccttta tatcgtaatt ttgtataatg cttccacagt gtcagatcta cccttctgta
     1861 aggaacgttg ttttgtgtaa agcagtcgaa gctcggctgt ctgtcatatc tgtgacgtgc
     1921 tgagggtgtg tcctgctcct ggattggagc tgcctcaccc taaacagtga cttcccattc
     1981 tccaaaaaca ccattacccg gttttcagct ctaactaggt gtcatgaggt ggcatcataa
     2041 acagagtttt cacgtcacaa agtcaaggcc taagttgcct aggataagat cttaaaagaa
     2101 aaaagaaaat taaacaaaaa gaacatttca tttttggctt tttcaaatta ttaaggaaaa
     2161 gtagcatttc tcaatggctt ttaggattat tcattgtagt ttccattttt actagaaagg
     2221 ttcttgtgta agccaagctg aactcagatg agctgtacat agattgtcct ggaactccca
     2281 agctcctatc ctgttcctaa gtgttgttat gacagatatg agccatcaca ccactggctc
     2341 tgctgctatt taataataag acatatttaa ttaaactaat attttaaaat tagggattct
     2401 ttaatcgtta atacttgtaa caaaaataaa tcaaaatcat gatgcagatg gaatttcagg
     2461 agactcccta caccttgttc cttaggcttg tctttctgcc gaaagtacca gaacctcact
     2521 agtcaccaat ttcaccactg tgccgcatac ctgatgccag taactttaca gtgacctaac
     2581 gttgggggag aacaagcttc cattggcagt caagggatgt ggtctctctg gttcttgcca
     2641 caactttctg cgtagtgttc ctttcatagc ttatgtctca aggagctgta gtcaagggag
     2701 ggagctgaga aagccaggtt aggagaactg ggagctgctt tgtgaaaccc catcctgagg
     2761 tggagactga gaccagagac attttactgt gtgttgtctg cagtttggag atggactttc
     2821 acttactatg actgcctaaa actctgaggt ttggagagta cttccataca tatgagagcc
     2881 ggtaagctac cccctgacct tgcctgccag ggcttagaac ccacattcct ggtaacttgt
     2941 cttgaactga gattttaaga ctgtcctgag agagtcagga atgcagaaga attcctaatg
     3001 cagtcactta agccagtgtc caagtgggct tgttatcctg tggcctacct tggttcagca
     3061 ggcaccttcc tacccatcat caggtctcca ggctgagtct gtcctgctgt gcatcttgcc
     3121 acagagaatc gtgctcttgg aagtctgaaa agcagttcaa cattagttct ggggcgggaa
     3181 ttctggttct tccaccagga gcagtggctg atgacattct tggtaaacgt cacccaatta
     3241 gtggagtacc ctttgttttt aagagatgta attctgtact ttttaatttt aaagattttg
     3301 ctgggttatc agagcgtaca agctggagga ttgttagatg gttgagggtt ctatggctgt
     3361 cactccatcc acagttgttc ctcacctttc actctgtgcc ttccctcttg ggcacctggt
     3421 tatctctctc agtttctata cttacctgct ctacacagct ttttaacctc ctcccaagac
     3481 gcccaggcct ctgaaaccac cattccttac tacagctagg agggcctggt gctcttgact
     3541 ctggtaatca gaatctgcaa tctgcatgtc aaatgtatac tggtcccagc ctttgcacct
     3601 cggcttctgc ccatgtccat ccttgtatag taaatctgtc tagaaacgct gtcttcctgc
     3661 aggccctgca gccactgcaa agcttgacgg ccttgccatt tctacaaggg tgctctccca
     3721 gcagtcacac tttgattgct gtgaggcaca tcctggattg tatttaaatg gcctgggtag
     3781 gctagcgctg ctctgctcac actgtctcat gtgaacagac cagaacagac tcctatacaa
     3841 agtctcataa tgtggggaag ccaaatgcca gctcctgtct atttccaccg tggagtcaaa
     3901 tacagggaat ttctcagtgt gtggtctatg agctgcatag agcagctttg taattaccct
     3961 ggctgtctgt tcatggtttt gcatttctct gtgacccaat gggtttcaca gcttcactcc
     4021 aaaatttgga gaattcaggg tagtcgtctt gttgctggaa agttagtagt tggaattatg
     4081 tggtgacaca ctccagttct gtgcttttgt gtggattctg atggagagtc taaagggagc
     4141 atgagttggt catttttctt ctaaaaacag tgttaaacta agagatgata aattagaatc
     4201 atttggacat tgatggtagg aggggtgtta gtaccaatgt cctgaaggtc acttggctat
     4261 ttactatccg tgggaaagct tgaaactgct tcctttgact taatacttat gcttgtaaga
     4321 gtcatttaac acaggttata atcaaagctt agtactgtta taaataagaa atttcctaat
     4381 gtgaatgaac agggaattgg tagataatct gtgctgtgat gtaatatcat accctaatgc
     4441 aatacaataa agtgctgcta atatggtaac aaaccagtgg gatgttggtg tccatggtgt
     4501 gacattaaag accaggaagc ctgggtgttt ttgacttcag tctttttata tttgtgtatt
     4561 ttctaacaat ttcacaacca acattgtaat gacaatattt ttatagaatg aaataaatat
     4621 ttataattta actattaaaa aaaaaaaaaa aaa
//
