LOCUS       NM_001081187            2181 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus HtrA serine peptidase 4 (Htra4), mRNA.
ACCESSION   NM_001081187 XM_284398
VERSION     NM_001081187.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 2181)
  AUTHORS   Patterson,V.L., Thompson,B.S., Cherry,C., Wang,S.B., Chen,B. and
            Hoh,J.
  TITLE     A Phenotyping Regimen for Genetically Modified Mice Used to Study
            Genes Implicated in Human Diseases of Aging
  JOURNAL   J Vis Exp (2016)
   PUBMED   27500671
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2181)
  AUTHORS   Liu,J., Li,Y. and Hoh,J.
  TITLE     Generation and characterization of mice with a conditional null
            allele of the HtrA4 gene
  JOURNAL   Mol Med Rep 12 (5), 6768-6774 (2015)
   PUBMED   26353049
  REMARK    GeneRIF: mice, featuring a conditional null allele of HtrA4, may
            provide a valuable tool to investigate the role of HtrA4 in
            development and pathogenesis of coronary heart disease and
            preeclampsia.
REFERENCE   5  (bases 1 to 2181)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB619816.1, BC145842.1, AK046614.1 and AV315397.2.
            
            On May 1, 2009 this sequence version replaced NM_001081187.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC145842.1, AK046614.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                BB619816.1         1-40
            41-1618             BC145842.1         1-1578
            1619-1658           AK046614.1         1538-1577
            1659-2181           AV315397.2         129-651
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 13.7 cM"
     gene            1..2181
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /note="HtrA serine peptidase 4"
                     /db_xref="GeneID:330723"
                     /db_xref="MGI:MGI:3036260"
     exon            1..572
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1540
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /note="high-temperature requirement factor A4; serine
                     protease HTRA4"
                     /codon_start=1
                     /product="serine protease HTRA4 precursor"
                     /protein_id="NP_001074656.1"
                     /db_xref="CCDS:CCDS40301.1"
                     /db_xref="GeneID:330723"
                     /db_xref="MGI:MGI:3036260"
                     /translation="MSFQRLWAVRTQFLLLWLLLPAVPVPWAEARRSRVSLPCPDACD
                     PTRCPTLPTCSAGLAPVPDRCGCCRVCAAAEGQECGGARGRPCAPRLRCGAPFSRDPS
                     GGAWLGTCGCAEGAEDAVVCGSDGRTYPSLCALRKENRAARQRGALPAVPVQKGACEE
                     AGTTRAGRLRRKYNFIAAVVEKVAPSVVHLQLFRRSPLTNQEIPSSSGSGFIVSEDGL
                     IVTNAHVLTNQQKIQVELQSGARYEATVKDIDHKLDLALIKIEPDTELPVLLLGRSSD
                     LRAGEFVVALGSPFSLQNTVTAGIVSTTQRGGRELGLKNSDIDYIQTDAIINHGNSGG
                     PLVNLDGDVIGINTLKVTAGISFAIPSDRIRQFLEDYHERQLKGKAPLQKKYLGLRML
                     PLTLNLLQEMKRQDPEFPDVSSGVFVYEVIQGSAAASSGLRDHDVIVSINGQPVTTTT
                     DVIEAVKDNDFLSIIVLRGSQTLFLTVTPEIIN"
     exon            573..672
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            673..877
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1072
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1105
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1220
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1278
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1374
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..2181
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2157..2162
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2180
                     /gene="Htra4"
                     /gene_synonym="B430206E18Rik"
                     /note="major polyA site"
ORIGIN      
        1 agactctgac ctttataaag tccgaaggct ggctgaggca gcatccaggc caggaaaaac
       61 agtcgaatcg acgaggtcag ggagcaagat gagcttccag cggttgtggg ctgtgcgaac
      121 acagtttctc ctgctgtggt tgctgctacc agccgtgcct gttccgtggg ccgaggccag
      181 gaggtccaga gtctcgctgc cctgtcccga tgcctgcgat cccacgcgct gcccgactct
      241 gcctacctgc tcggccggct tggcgccggt tcccgatcgc tgcggctgct gccgagtgtg
      301 cgccgcggcc gagggccagg agtgtggcgg ggcgcggggc cggccgtgcg ccccaaggct
      361 gcgctgcggc gccccgttct cccgggatcc ctcggggggc gcgtggctgg gcacctgcgg
      421 ctgcgcggag ggtgctgagg atgcggtagt gtgcggcagc gatgggcgca cctaccccag
      481 cctatgtgcg ctccgcaagg agaaccgagc cgctcgccag cggggcgcgc tcccggctgt
      541 gccagtgcag aagggtgcct gcgaggaagc agggaccaca agagcagggc ggctccggag
      601 aaagtacaac ttcatcgccg cagtggtgga gaaggtggcg ccgtctgtgg tccacttgca
      661 gctgttccgc agatcacctc tcaccaacca ggaaatccct tcctccagcg gctctgggtt
      721 catagtgtct gaggatgggc tcattgtcac caatgcccac gtcctcacca accagcagaa
      781 gatccaggta gagctccaga gcggggcccg gtatgaagcc accgtcaaag acatcgacca
      841 taaactggac cttgcactga ttaagatcga gccagatact gagcttccag tactgctgct
      901 gggccgatcc tctgacctcc gggctggaga gtttgtggta gctttgggca gcccattttc
      961 tctgcagaac accgtgactg cagggattgt cagcaccaca cagagaggcg gcagagagct
     1021 gggactgaag aattcagaca tagactatat ccagacggat gccatcatta accatggaaa
     1081 ttctgggggt ccgctggtga acttggatgg cgacgtgatt ggtataaaca ctctgaaggt
     1141 gactgcaggg atctcttttg cgatcccctc tgatcggatc agacagttcc tggaagacta
     1201 tcatgagcgc cagttgaaag gcaaggcccc tttgcagaag aaatacctgg gtcttcgaat
     1261 gctgcctctc actctgaacc tccttcagga aatgaagagg caagatccag agttccctga
     1321 tgtgagttct ggagtttttg tatatgaagt gattcaggga tcggctgctg caagctcggg
     1381 gttgagagac catgatgtaa ttgtcagcat aaacgggcaa cctgtcacca ccacaactga
     1441 tgtcattgaa gctgttaagg acaatgactt tctctccatc attgtgcttc gaggaagtca
     1501 aaccttgttt ctgacagtca cacctgaaat aatcaattaa gtatcttact ttgagaaact
     1561 gcctagcaaa accagttata ttacctggtt ttgtatcgaa gaggtgccag agatggcagg
     1621 gtcttctgga gatcaagaaa aatggatgct ttaaatgcag aagttcatgt ttgtgtgcat
     1681 acatcaacac acacacacac acacacacac acactcatgg atcctgaggt tgagagtgct
     1741 cttctgccgc aaaaaccttc ctaactcaaa tggaaacagc tatggtgatc tgataaaact
     1801 tgatgacagt aagaactgga aagcaggcaa ttcctaacta aatcttgata ggaaacttta
     1861 gttacctcct atacagccac aaactggtat gtcacgcaca tgtacacata attacctacc
     1921 aaatattaag aacctgaatc tggagtaaag aggtaatcac attttaaata ataccccttt
     1981 gtatactgaa tttcccaggt tatatccact ctgggccagg gtttgtggat agaaaggtca
     2041 tcacctataa gacatcttgg agctgatgac atcatactac cacacaggag tgtgatcatt
     2101 ttggaggtag aaacaatttc ggacctttag agtttctgag aatgtcttct atttctatta
     2161 aaataatttt tcgaaccgtt t
//
