LOCUS       NP_032339                388 aa            linear   ROD 27-APR-2025
DEFINITION  5-hydroxytryptamine receptor 4 isoform 1 [Mus musculus].
ACCESSION   NP_032339
VERSION     NP_032339.2
DBSOURCE    REFSEQ: accession NM_008313.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 388)
  AUTHORS   Hu,S., Kou,Y., Liu,X., Rong,W., Han,H. and Zhang,G.
  TITLE     Activation of the 5-hydroxytryptamine 4 receptor ameliorates tight
            junction barrier dysfunction in the colon of type 1 diabetic mice
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 55 (12), 1874-1883 (2023)
   PUBMED   37766457
  REMARK    GeneRIF: Activation of the 5-hydroxytryptamine 4 receptor
            ameliorates tight junction barrier dysfunction in the colon of type
            1 diabetic mice.
REFERENCE   2  (residues 1 to 388)
  AUTHORS   Karayol,R., Medrihan,L., Warner-Schmidt,J.L., Fait,B.W., Rao,M.N.,
            Holzner,E.B., Greengard,P., Heintz,N. and Schmidt,E.F.
  TITLE     Serotonin receptor 4 in the hippocampus modulates mood and anxiety
  JOURNAL   Mol Psychiatry 26 (6), 2334-2349 (2021)
   PUBMED   33441982
REFERENCE   3  (residues 1 to 388)
  AUTHORS   Bhattarai,Y., Jie,S., Linden,D.R., Ghatak,S., Mars,R.A.T.,
            Williams,B.B., Pu,M., Sonnenburg,J.L., Fischbach,M.A., Farrugia,G.,
            Sha,L. and Kashyap,P.C.
  TITLE     Bacterially Derived Tryptamine Increases Mucus Release by
            Activating a Host Receptor in a Mouse Model of Inflammatory Bowel
            Disease
  JOURNAL   iScience 23 (12), 101798 (2020)
   PUBMED   33299969
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 388)
  AUTHORS   Okumura,M., Hamada,A., Ohsaka,F., Tsuruta,T., Hira,T. and
            Sonoyama,K.
  TITLE     Expression of serotonin receptor HTR4 in glucagon-like
            peptide-1-positive enteroendocrine cells of the murine intestine
  JOURNAL   Pflugers Arch 472 (10), 1521-1532 (2020)
   PUBMED   32870377
  REMARK    GeneRIF: Expression of serotonin receptor HTR4 in glucagon-like
            peptide-1-positive enteroendocrine cells of the murine intestine.
REFERENCE   5  (residues 1 to 388)
  AUTHORS   Kobayashi,K., Mikahara,Y., Murata,Y., Morita,D., Matsuura,S.,
            Segi-Nishida,E. and Suzuki,H.
  TITLE     Predominant Role of Serotonin at the Hippocampal Mossy Fiber
            Synapse with Redundant Monoaminergic Modulation
  JOURNAL   iScience 23 (4), 101025 (2020)
   PUBMED   32283526
REFERENCE   6  (residues 1 to 388)
  AUTHORS   Claeysen,S., Sebben,M., Becamel,C., Bockaert,J. and Dumuis,A.
  TITLE     Novel brain-specific 5-HT4 receptor splice variants show marked
            constitutive activity: role of the C-terminal intracellular domain
  JOURNAL   Mol Pharmacol 55 (5), 910-920 (1999)
   PUBMED   10220570
REFERENCE   7  (residues 1 to 388)
  AUTHORS   Claeysen,S., Faye,P., Sebben,M., Taviaux,S., Bockaert,J. and
            Dumuis,A.
  TITLE     5-HT4 receptors: cloning and expression of new splice variants
  JOURNAL   Ann N Y Acad Sci 861, 49-56 (1998)
   PUBMED   9928238
  REMARK    Review article
REFERENCE   8  (residues 1 to 388)
  AUTHORS   Claeysen,S., Sebben,M., Journot,L., Bockaert,J. and Dumuis,A.
  TITLE     Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor
  JOURNAL   FEBS Lett 398 (1), 19-25 (1996)
   PUBMED   8946946
REFERENCE   9  (residues 1 to 388)
  AUTHORS   Ullmer,C., Schmuck,K., Kalkman,H.O. and Lubbert,H.
  TITLE     Expression of serotonin receptor mRNAs in blood vessels
  JOURNAL   FEBS Lett 370 (3), 215-221 (1995)
   PUBMED   7656980
REFERENCE   10 (residues 1 to 388)
  AUTHORS   Gerald,C., Adham,N., Kao,H.T., Olsen,M.A., Laz,T.M.,
            Schechter,L.E., Bard,J.A., Vaysse,P.J., Hartig,P.R., Branchek,T.A.
            et al.
  TITLE     The 5-HT4 receptor: molecular cloning and pharmacological
            characterization of two splice variants
  JOURNAL   EMBO J 14 (12), 2806-2815 (1995)
   PUBMED   7796807
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC152454.5 and AC131714.4.
            
            On Jan 5, 2006 this sequence version replaced NP_032339.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK141625.1, AK141587.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849389, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 35.1 cM"
     Protein         1..388
                     /product="5-hydroxytryptamine receptor 4 isoform 1"
                     /note="5-hydroxytryptamine receptor 4"
                     /calculated_mol_wt=43831
     Site            7
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P97288.3)"
     Region          20..323
                     /region_name="7tmA_5-HT4"
                     /note="serotonin receptor subtype 4, member of the class A
                     family of seven-transmembrane G protein-coupled receptors;
                     cd15056"
                     /db_xref="CDD:320184"
     Site            20..44
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P97288.3)"
     Region          21..47
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320184"
     Region          55..81
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320184"
     Site            55..78
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P97288.3)"
     Region          93..123
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320184"
     Site            93..117
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P97288.3)"
     Site            order(96,100..101,104..105,186,193,196..197,200,272,
                     275..276,279,294,298,302)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320184"
     Site            134..157
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P97288.3)"
     Region          136..159
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320184"
     Region          189..218
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320184"
     Site            189..212
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P97288.3)"
     Region          252..282
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320184"
     Site            258..283
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P97288.3)"
     Region          291..316
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320184"
     Site            291..314
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P97288.3)"
     CDS             1..388
                     /gene="Htr4"
                     /gene_synonym="5-HT-4; 5-HT4; 5-HT<4L>; 5HTR4"
                     /coded_by="NM_008313.4:213..1379"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS29291.1"
                     /db_xref="GeneID:15562"
                     /db_xref="MGI:MGI:109246"
ORIGIN      
        1 mdkldanvss negfrsvekv vlltflavvi lmailgnllv mvavcrdrql rkiktnyfiv
       61 slafadllvs vlvmpfgaie lvqdiwayge mfclvrtsld vllttasifh lccisldryy
      121 aiccqplvyr nkmtplrial mlggcwvlpm fisflpimqg wnnigivdvi ekrkfshnsn
      181 stwcvfmvnk pyaitcsvva fyipfllmvl ayyriyvtak ehaqqiqmlq ragatsesrp
      241 qpadqhsthr mrtetkaakt lcvimgcfcf cwapffvtni vdpfidytvp eqvwtaflwl
      301 gyinsglnpf lyaflnksfr rafliilccd derykrppil gqtvpcsttt ingsthvlrd
      361 tvecggqwes rchltatspl vaaqpsdt
//
