LOCUS       NM_001099644            2071 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus 5-hydroxytryptamine (serotonin) receptor 3A (Htr3a),
            transcript variant 2, mRNA.
ACCESSION   NM_001099644
VERSION     NM_001099644.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Felt,K., Stauffer,M., Salas-Estrada,L., Guzzo,P.R., Xie,D.,
            Huang,J., Filizola,M. and Chakrapani,S.
  TITLE     Structural basis for partial agonism in 5-HT3A receptors
  JOURNAL   Nat Struct Mol Biol 31 (4), 598-609 (2024)
   PUBMED   38177669
  REMARK    Erratum:[Nat Struct Mol Biol. 2024 Apr;31(4):728. doi:
            10.1038/s41594-024-01306-6.. PMID: 38580848]
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2071)
  AUTHORS   Cheffer,A., Garcia-Miralles,M., Maier,E., Akol,I., Franz,H.,
            Srinivasan,V.S.V. and Vogel,T.
  TITLE     DOT1L deletion impairs the development of cortical
            parvalbumin-expressing interneurons
  JOURNAL   Cereb Cortex 33 (19), 10272-10285 (2023)
   PUBMED   37566909
REFERENCE   4  (bases 1 to 2071)
  AUTHORS   Li,J., Zheng,S., Dong,Y., Xu,H., Zhu,Y., Weng,J., Sun,D., Wang,S.,
            Xiao,L. and Jiang,Y.
  TITLE     Histone Methyltransferase SETDB1 Regulates the Development of
            Cortical Htr3a-Positive Interneurons and Mood Behaviors
  JOURNAL   Biol Psychiatry 93 (3), 279-290 (2023)
   PUBMED   36335068
REFERENCE   5  (bases 1 to 2071)
  AUTHORS   Kastriti,M.E., Faure,L., Von Ahsen,D., Bouderlique,T.G.,
            Bostrom,J., Solovieva,T., Jackson,C., Bronner,M., Meijer,D.,
            Hadjab,S., Lallemend,F., Erickson,A., Kaucka,M., Dyachuk,V.,
            Perlmann,T., Lahti,L., Krivanek,J., Brunet,J.F., Fried,K. and
            Adameyko,I.
  TITLE     Schwann cell precursors represent a neural crest-like state with
            biased multipotency
  JOURNAL   EMBO J 41 (17), e108780 (2022)
   PUBMED   35815410
REFERENCE   6  (bases 1 to 2071)
  AUTHORS   Emerit,M.B., Martres,M.P., Miquel,M.C., el Mestikawy,S. and
            Hamon,M.
  TITLE     Differentiation alters the expression of the two splice variants of
            the serotonin 5-HT3 receptor-A mRNA in NG108-15 cells
  JOURNAL   J Neurochem 65 (5), 1917-1925 (1995)
   PUBMED   7595474
REFERENCE   7  (bases 1 to 2071)
  AUTHORS   Miyake,A., Mochizuki,S., Takemoto,Y. and Akuzawa,S.
  TITLE     Molecular cloning of human 5-hydroxytryptamine3 receptor:
            heterogeneity in distribution and function among species
  JOURNAL   Mol Pharmacol 48 (3), 407-416 (1995)
   PUBMED   7565620
REFERENCE   8  (bases 1 to 2071)
  AUTHORS   Hargreaves,A.C., Lummis,S.C. and Taylor,C.W.
  TITLE     Ca2+ permeability of cloned and native 5-hydroxytryptamine type 3
            receptors
  JOURNAL   Mol Pharmacol 46 (6), 1120-1128 (1994)
   PUBMED   7808432
REFERENCE   9  (bases 1 to 2071)
  AUTHORS   Hope,A.G., Downie,D.L., Sutherland,L., Lambert,J.J., Peters,J.A.
            and Burchell,B.
  TITLE     Cloning and functional expression of an apparent splice variant of
            the murine 5-HT3 receptor A subunit
  JOURNAL   Eur J Pharmacol 245 (2), 187-192 (1993)
   PUBMED   7683998
REFERENCE   10 (bases 1 to 2071)
  AUTHORS   Maricq,A.V., Peterson,A.S., Brake,A.J., Myers,R.M. and Julius,D.
  TITLE     Primary structure and functional expression of the 5HT3 receptor, a
            serotonin-gated ion channel
  JOURNAL   Science 254 (5030), 432-437 (1991)
   PUBMED   1718042
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY275174.1, AF513221.1 and CJ237116.1.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 3' coding region compared to variant 1. The resulting
            protein (isoform 2) is shorter but has the same N- and C-termini
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.176626.1, BC144799.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               BY275174.1         2-124
            124-1610            AF513221.1         1-1487
            1611-2071           CJ237116.1         51-511
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 26.53 cM"
     gene            1..2071
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /note="5-hydroxytryptamine (serotonin) receptor 3A"
                     /db_xref="GeneID:15561"
                     /db_xref="MGI:MGI:96282"
     exon            1..201
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..1586
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; 5-hydroxytryptamine receptor 3A; 5HT3 serotonin
                     receptor; serotonin receptor 3A; serotonin-gated ion
                     channel receptor"
                     /codon_start=1
                     /product="5-hydroxytryptamine receptor 3A isoform 2
                     precursor"
                     /protein_id="NP_001093114.1"
                     /db_xref="CCDS:CCDS90559.1"
                     /db_xref="GeneID:15561"
                     /db_xref="MGI:MGI:96282"
                     /translation="MRLCIPQVLLALFLSMLTAPGEGSRRRATQARDTTQPALLRLSD
                     HLLANYKKGVRPVRDWRKPTTVSIDVIMYAILNVDEKNQVLTTYIWYRQYWTDEFLQW
                     TPEDFDNVTKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYVHHRGEVQNYKPLQLVT
                     ACSLDIYNFPFDVQNCSLTFTSWLHTIQDINITLWRSPEEVRSDKSIFINQGEWELLE
                     VFPQFKEFSIDISNSYAEMKFYVIIRRRPLFYAVSLLLPSIFLMVVDIVGFCLPPDSG
                     ERVSFKITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETIFIVRL
                     VHKQDLQRPVPDWLRHLVLDRIAWILCLGEQPMAHRPPATFQANKTDDCSAMGNHCSH
                     VGGPQDLEKTPRGRGSPLPPPREASLAVRGLLQELSSIRHFLEKRDEMREVARDWLRV
                     GYVLDRLLFRIYLLAVLAYSITLVTLWSIWHYS"
     exon            202..368
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     exon            369..413
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     exon            414..523
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     exon            524..693
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     exon            694..854
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1065
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1287
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..2065
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2044..2049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /note="hexamer: AATAAA"
     polyA_site      2065
                     /gene="Htr3a"
                     /gene_synonym="5-HT3; 5-HT3A; 5-HT3R"
                     /note="major polyA site"
ORIGIN      
        1 aggtgcaggc gtgcagttgg aaaagcagtc tgcctgggac atgaggttgg cagcgggtgg
       61 cgtgcaggct ggcagtctgg gggactcatc ctgagtggct gctctgaggc cctcccacat
      121 ctgggaagct tgccatgcgg ctctgcatcc cgcaggtgct gttggccttg ttcctttcca
      181 tgctgacagc cccgggagaa ggcagccgga ggagggccac ccaggcccga gataccaccc
      241 agcctgctct actaaggctg tcagaccacc tcctggctaa ctacaagaag ggggtgcggc
      301 ctgtgcggga ctggaggaag cctactactg tctccattga tgtcatcatg tatgccatcc
      361 tcaacgtgga tgagaagaac caggttctga ccacctacat atggtaccgg cagtactgga
      421 ctgatgagtt tctgcagtgg actcctgagg acttcgacaa tgtcaccaaa ttgtccatcc
      481 ccacagacag catctgggtc cctgacatcc tcatcaatga gtttgtggac gtggggaagt
      541 ctccgaacat tccttatgtg tacgtgcatc atcgaggtga agttcagaac tacaagccct
      601 tgcaattggt gaccgcctgt agccttgaca tctacaactt cccctttgat gtgcagaact
      661 gctctctgac tttcaccagc tggctgcaca ccatccagga catcaacatt accctgtggc
      721 gatcaccgga agaagtgagg tctgacaaga gcatcttcat aaatcagggc gagtgggagc
      781 tgctggaggt gttcccccag ttcaaggagt tcagcataga tatcagtaac agctatgcag
      841 aaatgaagtt ctacgtgatc atccgccgga ggcctttatt ctacgcagtc agcctcttgc
      901 tgcccagtat cttcctcatg gtcgtggaca ttgtgggctt ttgcctgccc ccggacagcg
      961 gtgagagagt ctctttcaag atcacactcc ttctgggata ctcagtcttc ctcatcatcg
     1021 tgtcagacac actgccagca acggccatcg gtacccccct cattggtgtc tattttgtgg
     1081 tgtgcatggc tctgctagtg ataagcctcg ctgagaccat cttcattgtg cggctggtgc
     1141 ataagcagga cctacagcgg ccagtacctg actggctgag gcacctggtc ctagacagaa
     1201 tagcctggat actctgccta ggggagcagc ctatggccca tagaccccca gccaccttcc
     1261 aagccaacaa gactgatgac tgctcagcca tgggaaacca ctgcagccat gttggaggac
     1321 ctcaggactt ggagaagacc ccaaggggca gaggtagccc tcttccacca ccaagggagg
     1381 cctcgctggc tgtgcgcggt ctcttgcaag agctatcctc catccgccac ttcctggaga
     1441 agcgggatga gatgcgggag gtggcaaggg actggctgcg ggtgggatac gtgctggaca
     1501 ggctgctgtt ccgcatctac ctgctggctg tgctcgctta cagcatcacc ctggtcactc
     1561 tctggtccat ttggcattat tcttgagtgg gcacagcctg acagggaggg gctatggaac
     1621 tggttaggat aagtatgggg gagttccctc ttaggtcctg catcctgttt ccaacaccaa
     1681 ttcatctgag caaccccagt ctctttgtcc cctaaactta gtgctgaaga cccagtctgt
     1741 ctctctgact tccctatcat ggctttaaag catgatatcc tagatcaaga gaaaccaagt
     1801 accctctaac cttacttatc agggcgtcaa gccctggttt ccttaccagt acttctgtga
     1861 ttacggcccc tggaactgct catttccaca tttttttttt ctgatcaaag aaaagcaaat
     1921 tgtcttgcat agatgcctga gacttctgca cttattttat ctagccccta atggctccct
     1981 cttcagctca cctgtgggta cttccctaac actcagcttc atcagccaat gggagcaggg
     2041 gataataaaa tgctatgata tcttaaaaaa a
//
